scholarly journals Extraction of Capsinoid and its Analogs From Pepper Waste of Different Genotypes

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986567
Author(s):  
Sibel Bayil Oğuzkan
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Value Added ◽  
Sweet Pepper ◽  
Bioactive Materials ◽  
Waste Products ◽  
Cultivated Plant ◽  
Solanaceae Family ◽  
Pepper Sample

Pepper, a member of the Capsicum genus of the Solanaceae family, is an annual, cultivated plant that grows in temperate climates. The main analogs of capsinoids, which are the secondary metabolites of peppers, are capsaicin and dihydrocapsaicin. During process, calyxes and peduncles are considered to be waste. Levels of capsinoids in these tissues in not well known. An optimized method was used to extract bioactive materials from the waste products from C. annum L. genotypes. Calyxes and peduncles were collected and dried in the shade. Extractions were in MeOH solutions and the extracts were analyzed using high-performance liquid chromatography (HPLC) to quantify the total amount of capsinoid, capsaicin, and dihydrocapsaicin contents. Capsinoid, and its analogs, were identified in all genotypes at varying concentrations, and their capsaicin and dihydrocapsaicin contents evidenced with liquid chromatography-mass spectrometry (LC-MS). In conclusion, the highest amount of capsinoids was found in chili Samandağ peppers, whereas the lowest amount of capsinoids was found in the red sweet pepper sample. All pepper genotypes, the capsaicin amount was higher comparison to dihydrocapsaicin. Capsanoids and anologues obtained from waste pepper can be used as raw materials in production of value added products.

Specific serum quinidine assay by high-performance liquid chromatography.

1977 ◽  
Vol 23 (11) ◽  
pp. 2030-2033 ◽  
Author(s):  
W G Crouthamel ◽  
B Kowarski ◽  
P K Narang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Therapeutic Index ◽  
Assay Method ◽  
Cardioactive Drug ◽  
Double Extraction ◽  
Serum Quinidine

Abstract The cardioactive drug quinidine has a narrow therapeutic index; consequently, determination of serum quinidine concentrations can be important. We describe a relatively rapid and specific assay for quinidine in serum by high-performance liquid chromatography. It is suitable for use in patient monitoring or pharmacodynamic studies. Alkalinized serum is extracted with benzene, which is evaporated under nitrogen and reconstituted with methanol; an aliquot is chromatographed. Quinidine is separated from its metabolites and dihydroquinidine (a contaminant in quinidine raw materials). The retention time for quinidine is 4 min 10 s, for dihydroquinidine it is 5.5 min. Results for patients' sera by this assay method and the double-extraction method of Cramer and Isaksson [Scand. J. Clin. Lab. Invest. 15, 553 (1963] correlate well (r = .975).

scholarly journals Single Laboratory Validation of a Method for Determination of Glucosamine in RawMaterials and Dietary Supplements Containing Glucosamine Sulfate and/or Glucosamine Hydrochloride by High-Performance Liquid Chromatography with FMOC-Su Derivatization

2004 ◽  
Vol 87 (5) ◽  
pp. 1083-1092 ◽  
Author(s):  
Joseph ZiQi Zhou ◽  
Ted Waszkuc ◽  
Felicia Mohammed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
High Performance ◽  
Raw Materials ◽  
Glucosamine Sulfate ◽  
Glucosamine Hydrochloride ◽  
Relative Standard ◽  
Single Laboratory

Abstract Single laboratory validation of a method for determination of glucosamine in raw materials and dietary supplements containing glucosamine sulfate and/or glucosamine hydrochloride by with high-performance liquid chromatography FMOC-Su derivatization. Tests with 2 blank matrixes containing SAMe, vitamin C, citric acid, chondroitin sulfates, methylsulfonylmethane, lemon juice concentrate, and other potential interferents showed the method to be selective and specific. Eight calibration curves prepared over 7 working days indicated excellent reproducibility with the linear range at least over 2.0–150 μg/mL, and determination coefficients >0.9999. Average spike recovery from the blank matrix (n = 8 over 2 days) was 93.5, 99.4, and 100.4% at respective spike levels of 15, 100, and 150%, and from the sample matrix containing glucosamine (n = 3) was 99.9 and 102.8% at respective levels of 10 and 40%, with relative standard deviations <0.9%. The method was also applied to 12 various glucosamine finished products and raw materials. The stability tests confirmed that glucosamine–FMOC-Su derivative once formed is stable at room temperature for at least 5 days. Limit of quantitation was 1 μg/mL and limit of detection was 0.3 μg/mL. The method is ready to proceed for the collaborative study.

scholarly journals Evalution of Substitution of Bark With Small Branches of Stereospermum Chelonoides DC and Quantitative Estimation of Lapachol By HPLC

2021 ◽  
Author(s):  
Ajay Kumar Meena ◽  
Ilavarasan R. ◽  
Om Prakash ◽  
Ayyam Perumal ◽  
Ravindra Singh ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Estimation ◽  
Harmful Effect ◽  
Stem Bark ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Quality Control Purpose

Abstract Background: Stereospermum chelonoides DC, also known as padhri, is a medicinal plant that belongs to the Bignoniaceae family and is commonly used in herbal medicine. Natural materials separated from a variety of traditional herbal plants have been claimed to have had a significant impact on the development of numerous lead compounds used in the treatment of health issues in communities all over the world. The plant stem bark and small branches is used in traditional and folklore medicine to treat a variety of ailments. The aim of this study is to assess the pharmacognostic and physicochemical analysis of Stereospermum chelonoides stem bark and small branches. The stem is used for the study of characters; whereas physicochemical parameters such as pH, total ash content, acid insoluble ash, water soluble extractive, alcohol soluble extractive, loss on drying are determined. Results: Preliminary phytochemical studies of ethanolic and aqueous extract of Stereospermum chelonoides DC ascertained the presence of flavonoids, saponins, Coumarins, Alkaloids, Furanoids, phenolic, Quinones, Reducing sugars, Sugars (Carbohydrate), triterpenoids, and tannins. The findings of the present study lay down standards which could be useful to detect the authenticity and for detailed evaluation and investigation of this plant. Lapachol which is indicated in the High Performance Liquid Chromatography, further studies may be conducted on the method validation by High Performance Liquid Chromatography to quantify in formulations, extracts and raw materials for quality control purpose. Conclusion: The stem bark and small branches of this plant useful in the future as it will not have any harmful effect to the plant so its small branches are more important than stem bark in the formulation of drugs which will derive from Stereospermum chelonoides DC.

scholarly journals An efficient and metal-free synthesis of 5,7-dimethylcyclopentenon[2,3-c]coumarin

2021 ◽  
pp. 174751982110467
Author(s):  
Thirupathi Reddy Yerramreddy ◽  
Alexandros Yiannikouris
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Raw Materials ◽  
Reaction Times ◽  
Metal Free ◽  
H Nmr ◽  
Molecular Imprinting Polymers ◽  
Viable Approach

An improved and alternative method for the metal-free synthesis of 5,7-dimethylcyclopentenon[2,3- c]coumarin as a possible useful aflatoxin template in the production of molecular imprinting polymers with affinity toward natural aflatoxin molecules is reported. A synthesis of 5,7-dimethylcyclopentenon[2,3- c]coumarin from 3,5-dimethoxyphenol via a Pechmann condensation involved five steps and represents an alternative commercially viable approach over existing synthetic protocols in the literature. Operational simplicity, inexpensive key raw materials, short reaction times, and excellent yields are remarkable features of this approach. The aflatoxin template was provided in 55%–67% overall yield from 3,5-dimethoxyphenol with >99% purity, evaluated by high-performance liquid chromatography, 1H NMR, 13C NMR, high-resolution mass spectrometry, and liquid chromatography–mass spectrometry.

scholarly journals Control of animal products contamination with nitroimidazole group antibiotics

2020 ◽  
Vol 222 ◽  
pp. 06019
Author(s):  
Alexandr Prosekov ◽  
Tatiana Podlegaeva ◽  
Olga Chaplygina ◽  
Oksana Kozlova ◽  
Anna Tulaeva
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Muscle Tissue ◽  
High Performance ◽  
Raw Materials ◽  
Mass Spectrometric ◽  
Tissue Samples ◽  
Animal Products ◽  
Regulatory Documents

Antibiotic contamination of water and food is a particularly important environmental worldwide problem. Antibiotics are found in water, soil, and food, which negatively affects both the environment and the health of the entire world’s population. Therefore, it is important to control the content of antimicrobials. To solve this problem, scientists are developing new and improving existing methods for these preparation determination. The paper considers the possibility of applying the technique of high-performance liquid chromatography with mass spectrometric detection for the determination of nitroimidazole group antibiotics in meat raw materials. The method was evaluated in terms of the main characteristics taken into account during validation: accuracy, reproducibility, linearity, and specificity. Studies conducted on pork muscle tissue samples showed the absence of antibiotics of this group in all samples, which meets the requirements of regulatory documents.

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