scholarly journals Chemical Constituents From the Fruits of Xanthium strumarium and Their Antitumor Effects

2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2094554
Author(s):  
Chao Tong ◽  
Ri-Hui Chen ◽  
Ding-Cheng Liu ◽  
De-Sheng Zeng ◽  
Hui Liu
Keyword(s):  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Hepatoma Cell ◽  
Cancer Cell Line ◽  
Xanthium Strumarium ◽  
Hepatoma Cell Line ◽  
Human Gastric Cancer ◽  
Antitumor Effects ◽  
Mcf 7

A new neolignan, (7 S,8 R)- threo-1′-[3′-hydroxy-7-(4-hydroxy-3,5-dimethoxyphenyl)-8-hydroxymethyl-7,8-dihydrobenzofuran]acrylaldehyde (1), along with 5 known compounds 2-6, were isolated from the fruits of Xanthium strumarium. Their structures were elucidated by extensive spectroscopic methods. All the isolates were evaluated for in vitro cytotoxicity against human cancer cell lines, including human hepatoma cell line (HepG2), human breast cancer cell line (MCF-7), human colon cancer cell line (HCT-116), and human gastric cancer cell line (SGC-7901). Among them, compounds 1 and 3 showed selective cytotoxicity on HepG2 cancer cells with half-maximal inhibitory concentration (IC50) values of 10.2 ± 1.2 and 18.3 ± 1.6 μM, respectively. Moreover, compound 5 also exhibited moderate cytotoxicity against MCF-7 cancer cells with an IC50 value of 20.5 ± 1.3 μM.

A Novel Triazole Derivative Drug Presenting In Vitro and In Vivo Anticancer Properties

2018 ◽  
Vol 18 (17) ◽  
pp. 1483-1493
Author(s):  
Ricardo Imbroisi Filho ◽  
Daniel T.G. Gonzaga ◽  
Thainá M. Demaria ◽  
João G.B. Leandro ◽  
Dora C.S. Costa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Hepatic Function ◽  
Anticancer Properties ◽  
Mcf 7

Background: Cancer is a major cause of death worldwide, despite many different drugs available to treat the disease. This high mortality rate is largely due to the complexity of the disease, which results from several genetic and epigenetic changes. Therefore, researchers are constantly searching for novel drugs that can target different and multiple aspects of cancer. Experimental: After a screening, we selected one novel molecule, out of ninety-four triazole derivatives, that strongly affects the viability and proliferation of the human breast cancer cell line MCF-7, with minimal effects on non-cancer cells. The drug, named DAN94, induced a dose-dependent decrease in MCF-7 cells viability, with an IC50 of 3.2 ± 0.2 µM. Additionally, DAN94 interfered with mitochondria metabolism promoting reactive oxygen species production, triggering apoptosis and arresting the cancer cells on G1/G0 phase of cell cycle, inhibiting cell proliferation. These effects are not observed when the drug was tested in the non-cancer cell line MCF10A. Using a mouse model with xenograft tumor implants, the drug preventing tumor growth presented no toxicity for the animal and without altering biochemical markers of hepatic function. Results and Conclusion: The novel drug DAN94 is selective for cancer cells, targeting the mitochondrial metabolism, which culminates in the cancer cell death. In the end, DAN94 has been shown to be a promising drug for controlling breast cancer with minimal undesirable effects.

miRNAs expressed differently in cancer stem cells and cancer cells of human gastric cancer cell line MKN-45

2012 ◽  
Vol 30 (5) ◽  
pp. 411-418 ◽  
Author(s):  
Azadeh Fahim Golestaneh ◽  
Amir Atashi ◽  
Lida Langroudi ◽  
Abbas Shafiee ◽  
Nasser Ghaemi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer

Effect of zoledronic acid combined with cisplatin and paclitaxel on inhibition of non-small cell lung cancer cell lines

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e19095-e19095
Author(s):  
Z. Gao ◽  
B. Han ◽  
J. Teng
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Zoledronic Acid ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell ◽  
Antitumor Effects

e19095 Backgrounds: Recent studies reported that zoledronic acid, a biphosphonate with proposed apoptotic activity, could cause a direct antitumor effect. Our prior study [J Clin Oncol 26: 2008 (May 20 suppl; abstr 19116)] reported that Zoledronic acid combined with Cisplatin shows significantly synergistic antitumor effects on lung cancer cell line A549 and subcutaneous implanted tumor on nude mice. Investigate whether zoledronic acid, augmented the cytotoxicity of cisplatin and/or paclitaxel in A549 lung cancer cell line. Methods: This cell line was subjected to different concentrations of the above chemotherapeutic agents and zoledronic acid. Cytotoxicity was assessed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide) assay. Flow cytometry was used to examine cell cycle,cell apoptosis rate. Results: Zoledronic acid in in 50 micromolar (mM) concentration augmented the cytotoxicity by cisplatin in 10μmol/L and paclitaxel in 5μmol/L. Zoledronic acid could inhibit the proliferation of lung cancer cells in vitro,which was associated with arresting of G1 phase and inducing apoptosis by a time-dependent and dose-dependentmanner. The apoptosis rate of cell increased after zoledronic acid combined with cisplatin and/or paclitaxel. Conclusions: Zoledronic acid can induce apoptosis and block cell cycle of lung cancer cells. Zoledronic acid has also shown synergistic antitumor effects when combined with cisplatin and/or paclitaxel. The clinical potential of this finding should be further studied. No significant financial relationships to disclose.

scholarly journals Cellular uptake and toxicity of gold nanoparticles in a tumor-like (hypoxic) environment

2021 ◽  
Author(s):  
Mehrnoosh Neshatian
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Drug Carrier ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Hypoxic Conditions ◽  
Hypoxic Environment ◽  
Mcf 7

Cancer cells deprived adequate oxygen tension, are called hypoxic cells. Hypoxic shows resistance to both chemotherapy and/or radiotherapy. On the other hand gold Nanoparticles (GNPs) are being used as promising agents in cancer therapy. GNPs can be used as radiosensitizer and drug carrier agents. It also has been show that uptake of GNPs in normal oxygenated (normoxic) cancer cells is maximum for 50 nm GNPs. However, it is important to know the variation in GNPs uptake and toxicity of 50 nm particles in a tumor-like (hypoxic) environment. Hence, we have investigated toxicity and uptake of 50 nm GNPs in hypoxic cancer cells. MCF-7 (breast cancer cell line) and HeLa (cervical cancer cell line), were used in this study. The results of this study showed that uptake of GNPs in hypoxic cells were dependent on the exposure duration to hypoxic conditions. Cancer cells under prolong hypoxia showed highest GNP uptake.

Investigation of the anti-cancer effects of free and PLGA-PAA encapsulated Hydroxytyrosol on the MCF-7 breast cancer cell line

2020 ◽  
Vol 20 ◽  
Author(s):  
Maryam Safi ◽  
Habib Onsori ◽  
Mohammad Rahmati
Keyword(s):  
Breast Cancer ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Anti Cancer ◽  
Mcf 7

Purpose: Breast cancer is the most frequent cancer among women and the most important cause of death. Surgery and chemotherapy are the common treatment of the breast cancer, but increasing drug resistance has created many challenges in its treatment. The present study aimed to investigate the anti-cancer function of free and nano-encapsulated hydroxytyrosol on the MCF-7 breast cancer cell line. Methods: The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hydroxytyrosol was synthesized, and the MTT assay was performed to evaluate the anti-proliferative and anti-tumor effects of both free and nanoencapsulated Hydroxytyrosol. After the extraction of RNA from the treated and control cancer cells, cDNA synthesis was performed and the expression of P21, P27, and Cyclin D1 genes were evaluated by Real-Time PCR. Results: The results of the study showed that free (12 ppm and 72 hours) and nano-encapsulate (10 ppm and 24 hours) hydroxytyrosol resulted in 50% death (IC50) of the cancer cells and increased by increasing the concentration and time. Also, free and nano-encapsulated hydroxytyrosol increased the expression of P21 and P27 genes and reduced the expression of Cyclin D1 in breast cancer cells. In general, the nano-encapsulated hydroxytyrosol showed more anticancer function than the free hydroxytyrosol. Conclusion: The present study illustrated that the hydroxytyrosol could lead to the cell death in MCF-7 breast cancer by regulating the cell cycle. Also, the nano-encapsulation of Hydroxytyrosol enhanced the Hydroxytyrosol anticancer function by PLGA-co-PAA. However, for more accurate results, further studies on animal models are necessary.

scholarly journals Cellular uptake and toxicity of gold nanoparticles in a tumor-like (hypoxic) environment

2021 ◽  
Author(s):  
Mehrnoosh Neshatian
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Drug Carrier ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Hypoxic Conditions ◽  
Hypoxic Environment ◽  
Mcf 7

Cancer cells deprived adequate oxygen tension, are called hypoxic cells. Hypoxic shows resistance to both chemotherapy and/or radiotherapy. On the other hand gold Nanoparticles (GNPs) are being used as promising agents in cancer therapy. GNPs can be used as radiosensitizer and drug carrier agents. It also has been show that uptake of GNPs in normal oxygenated (normoxic) cancer cells is maximum for 50 nm GNPs. However, it is important to know the variation in GNPs uptake and toxicity of 50 nm particles in a tumor-like (hypoxic) environment. Hence, we have investigated toxicity and uptake of 50 nm GNPs in hypoxic cancer cells. MCF-7 (breast cancer cell line) and HeLa (cervical cancer cell line), were used in this study. The results of this study showed that uptake of GNPs in hypoxic cells were dependent on the exposure duration to hypoxic conditions. Cancer cells under prolong hypoxia showed highest GNP uptake.

Sign in / Sign up

Export Citation Format

Share Document