scholarly journals Araliaarmoside: A New Triterpene Glycoside Isolated From the Leaves of Aralia armata

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095330
Author(s):  
Pham Hai Yen ◽  
Nguyen Thi Cuc ◽  
Phan Thi Thanh Huong ◽  
Nguyen Xuan Nhiem ◽  
Nguyen Thị Hong Chuong ◽  
...  
Keyword(s):  
Oleanolic Acid ◽  
Triterpene Glycoside ◽  
Spectroscopic Data ◽  
Inhibitory Concentration ◽  
In Vitro Assay ◽  
Ionization Mass ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Hepg2 Cell Lines

One new oleanane-type triterpene glycoside, oleanolic acid-[28 -O-β-d-glucopyranosyl]-3 -O-[ β-d-glucopyranosyl(1→6)- β-d-glucopyranosyl](1→3)[ α-l-arabinofuranosyl(1→4)]- β-d-glucuronopyranoside (1), and 3 known ones {oleanolic acid-[28 -O-β-d-glucopyranosyl]-3 -O-[ β-d-galactopyranosyl(1→3)]-[ β-d-glucopyranosyl(1→2)]- β-d-glucuronopyranoside (2) chikusetsusaponin IVa methyl ester (3), and chikusetsusaponin IV (4)} were isolated from the leaves of Aralia armata. Their chemical structures were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, 1-dimensional and 2-dimensional nuclear magnetic resonance (NMR) spectral data, as well as comparison with data in the previous literature. This is the first report of full NMR spectroscopic data of 2. Compounds 1-4 displayed weak cytotoxic activity toward KB and HepG2 cell lines, with half-maximal inhibitory concentration50 values ranging from 24.2 ± 0.3 to 32.6 ± 0.8 µM in in vitro assay.

scholarly journals Araliachinoside A: A New Triterpene Glycoside From Aralia chinensis Leaves

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095275
Author(s):  
Pham Hai Yen ◽  
Nguyen Thi Cuc ◽  
Phan Thi Thanh Huong ◽  
Nguyen Xuan Nhiem ◽  
Nguyen Thi Hoai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Resolution ◽  
Inhibitory Concentration ◽  
In Vitro Assay ◽  
Ionization Mass ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Hepg2 Cell Lines

From the leaves of Aralia chinensis, 3 oleanane-type triterpene glycosides have been isolated, including 1 new glycoside, 3β,23 -dihydroxyolean-12-ene-28-oic acid 3 -O-β-d-glucopyranosyl-(1→3)- α-l-arabinopyranosyl-(1→3)-β-d-glucuronopyranoside 28 -O-β-d-glucopyranosyl ester (named as araliachinoside A, 1), and 2 known ones, 3β,23 -dihydroxyolean-12-ene-28-oic acid 3 -O-α-l-arabinopyranosyl-(1→3)- β-d-glucuronopyranoside 28 -O-β-d-glucopyronosyl ester (2) and 3β-hydroxyolean-12-ene-28-oic acid 3 -O-β-d-glucurono pyranoside 28 -O-β-d-glucopyronosyl ester (3). Their chemical structures were elucidated by using a combination of high-resolution electrospray ionization-mass spectrometry, 1-dimensional and 2-dimensional nuclear magnetic resonance spectral data, and by comparison with previous literature. Compounds 1-3 displayed cytotoxic activity toward KB and HepG2 cell lines with half-maximal inhibitory concentration values ranging from 8.1 ± 0.1 to 15.7 ± 0.3 µM in in vitro assay.

scholarly journals Aramatosides C and D, 2 Previously Undescribed Triterpene Glycosides Isolated From the Roots of Aralia armata

2021 ◽  
Vol 16 (7) ◽  
pp. 1934578X2110336
Author(s):  
Nguyen T. Hong Chuong ◽  
Do T. Thuy Van ◽  
Giang T. Kim Lien ◽  
Pham H. Yen ◽  
Dan T. Thuy Hang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Positive Control ◽  
In Vitro Assay ◽  
Triterpene Glycosides ◽  
Ionization Mass ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Control Compound ◽  
Hepg2 Cell Lines

The 2 new oleanane-type triterpene glycosides, 23-hydroxyoleanolic acid-[28- O- β-D-glucopyranosyl]-3- O-{ β-D-glucopyranosyl-(1→2)-[ β-D-glucopyranosyl-(1→3)]- β-D-galactopyranoside}, (1) and oleanolic acid-[28- O- β-D-glucopyranosyl]-3- O-{ β-D-glucopyranosyl-(1→2)-[ β-D-glucopyranosyl-(1→3)]- β-D-galactopyranoside} (2) were isolated from the roots of Aralia armata. Their chemical structures were elucidated by using a combination of high resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1 dimensional (1D), and 2 dimensional (2D) nuclear magnetic resonance spectral data, as well as by comparison with the previous literature. Compounds 1 and 2 displayed weak cytotoxic activity toward KB and HepG2 cell lines with IC50 values of 25.1 ± 1.2 and 23.7 ± 0.9 µM (for 1) and 29.5 ± 1.3 and 23.9 ± 0.7 µM (for 2), respectively, compared to that of the positive control compound, ellipticine (IC50: 1.3 ± 0.1 and 1.6 ± 0.1 µM, respectively) in in vitro assay.

scholarly journals PTP1B INHIBITORS FROM ISODON TERNIFOLIUS COLLECTED IN VIETNAM

2020 ◽  
Vol 58 (5) ◽  
pp. 533
Author(s):  
Nguyen Phi-Hung
Keyword(s):  
Enzyme Activity ◽  
Inhibitory Activity ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Whole Plant ◽  
Ic50 Values ◽  
Ptp1b Inhibitors ◽  
First Time

From the whole plant of Isodon ternifolius collected in Vietnam, four triterpens including ursaldehyde (1), ursolic acid (2), b-sitosterol (3) and b-sitosteryl ferulate (4) were purified. Their chemical structures were determined by interpretation of NMR and MS data and comparison with the literatures. Compounds 1-4 were evaluated for their inhibitory activity against PTP1B enzyme activity using in vitro assay. Compounds 1 and 2 displayed potential activities with IC50 values of 16.92 ± 0.12 and 3.42 ± 0.45 μM, respectively. This is the first time that compounds 1 and 4 have been isolated from the Isodon genus and I. ternifolius has been evaluated for the PTP1B inhibitory activity.

scholarly journals A new 11,10-guaiane-type sesquiterpenoid from the roots of Stellera chamaejasme Linn

2020 ◽  
pp. 174751982096104
Author(s):  
Fei-Feng Hu ◽  
Dan-Dan Qi ◽  
Sha Xu ◽  
Wei Mao
Keyword(s):  
Spectroscopic Analysis ◽  
Triple Negative ◽  
Cancer Cell Line ◽  
In Vitro Assay ◽  
Resonance Spectroscopy ◽  
Ionization Mass ◽  
Vitro Assay ◽  
Stellera Chamaejasme ◽  
First Time

Chamaejasmone F, a new 11,10-guaiane-type sesquiterpenoid, is isolated from the roots of Stellera chamaejasme Linn., along with four known compounds including two guaiane sesquiterpenoids and two lignans. The structure of the new compound is elucidated by extensive spectroscopic analysis, especially two-dimensional nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometry. The known compounds 1β-hydroxy-10βH-guaia-4,11-dien-3-one and (+)-hinokinin are isolated from the genus Stellera for the first time. In an in vitro assay, chamaejasmone F shows moderate cytotoxic activity against the human triple negative breast cancer cell line MB-MDA-231.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Angiogenic Effect of Pravastatin Alone and with Sera from Healthy and Complicated Pregnancies Studied by in vitro Vasculogenesis/Angiogenesis Assay

2021 ◽  
pp. 1-9
Author(s):  
Anita Virtanen ◽  
Outi Huttala ◽  
Kati Tihtonen ◽  
Tarja Toimela ◽  
Tuula Heinonen ◽  
...  
Keyword(s):  
Direct Effect ◽  
Late Onset ◽  
Maternal Serum ◽  
In Vitro Assay ◽  
Serum Samples ◽  
Therapeutic Concentration ◽  
Tubule Formation ◽  
Vitro Assay ◽  
Angiogenesis Assay

<b><i>Objective:</i></b> To determine the direct effect of pravastatin on angiogenesis and to study the interaction between pravastatin and maternal sera from women with early- or late-onset pre-eclampsia (PE), intrauterine growth restriction, or healthy pregnancy. <b><i>Methods:</i></b> We collected 5 maternal serum samples from each group. The effect of pravastatin on angiogenesis was assessed with and without maternal sera by quantifying tubule formation in a human-based in vitro assay. Pravastatin was added at 20, 1,000, and 8,000 ng/mL concentrations. Concentrations of angiogenic and inflammatory biomarkers in serum and in test medium after supplementation of serum alone and with pravastatin (1,000 ng/mL) were measured. <b><i>Results:</i></b> Therapeutic concentration of pravastatin (20 ng/mL) did not have significant direct effect on angiogenesis, but the highest concentrations inhibited angiogenesis. Pravastatin did not change the levels of biomarkers in the test media. There were no changes in angiogenesis when therapeutic dose of pravastatin was added with maternal sera, but there was a trend to wide individual variation towards enhanced angiogenesis, particularly in the early-onset PE group. <b><i>Conclusions:</i></b> At therapeutic concentration, pravastatin alone or with maternal sera has no significant effect on angiogenesis, but at high concentrations the effect seems to be anti-angiogenic estimated by in vitro assay.

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