The study of intralobular heterogeneity of enzyme activity in livers with the use of invalid histochemical methods.

Quantitative histochemical methods were used for assaying several dehydrogenases in various cell zones of the rachitic rat epiphyseal cartilage during healing brought about by either vitamin D administration or fasting for 48 hr. 6-Phosphogluconic dehydrogenase activity was significantly greater in the cells of both the proliferating and hypertrophic zones of rachitic control rats as compared to those from vitamin D-treated or fasted rats. Activity of 6-phosphogluconic dehydrogenase in the cells from proliferating and hypertrophic zones was identical in the different groups. Lactic dehydrogenase activity of proliferating and hypertrophic cells obtained from rachitic controls was markedly higher than those from the fasted animals. Minor differences were noted in the malic and isocitric dehydrogenases and peptidase in the cartilage cells obtained from vitamin D-treated, untreated and fasted rats.

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A CRITICAL EVALUATION OF THE HISTOCHEMICAL METHODS FOR CARBONIC ANHYDRASE

Journal of Histochemistry & Cytochemistry ◽

10.1177/20.5.319 ◽

1972 ◽

Vol 20 (5) ◽

pp. 319-330 ◽

Cited By ~ 47

Author(s):

THOMAS F. MUTHER

Keyword(s):

Enzyme Activity ◽

Surface Layer ◽

Carbonic Anhydrase ◽

Sodium Lauryl Sulfate ◽

Critical Evaluation ◽

Lauryl Sulfate ◽

Carbonic Anhydrase Inhibitors ◽

Histochemical Methods ◽

Rate Limiting

The histochemical methods for carbonic anhydrase are not based on the postulated dehydration of HCO3–. The staining is caused by the formation of an unknown reactive Co compound in the surface layer secondary to enzyme-independent alkalinization of the medium. Kinetic analysis of the reaction shows that loss of CO2 from the medium is rate-limiting. Carbonic anhydrase inhibitors delay the staining by interacting with Co and not by inhibiting the enzyme; they are effective when used after the reaction is complete. The reaction can also be inhibited by agents which are not carbonic anhydrase inhibitors, such as sodium lauryl sulfate and 5-aminothiadiazole, but not by in vivo administered acetazolamide. A comparison of the effect of various fixatives on the biochemical and histochemical enzyme activity shows no correlation. While carbonic anhydrase itself is stained by the reaction, the methods lack the claimed specificity for it.

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A CLINICAL AND HISTOCHEMICAL STUDY OF DISORDERS OF THE HUMAN TESTES

Acta Endocrinologica ◽

10.1530/acta.0.0550427 ◽

1967 ◽

Vol 55 (3) ◽

pp. 427-439 ◽

Cited By ~ 7

Author(s):

Joh. Koudstaal ◽

E. L. Frensdorf ◽

J. Kremer ◽

J. M. Mudde ◽

M. J. Hardonk

Keyword(s):

Enzyme Activity ◽

Sertoli Cell ◽

Sertoli Cells ◽

Histochemical Study ◽

Interstitial Cells ◽

Inhibiting Effect ◽

Histochemical Methods ◽

High Enzyme ◽

Long Time ◽

High Enzyme Activity

ABSTRACT The testes of 50 subfertile and infertile males were investigated by clinical and histochemical methods. The enzyme activity per cell of interstitial cells of normal and abnormal testes was about the same. The output of the gonadotrophins of men with seriously damaged testes may be increased; the output of the oestrogens was normal and the output of 17-ketosteroids was normal or slightly decreased. Some of these patients showed a poor development of growth of the beard. The Sertoli cell is very resistant to atrophy and keeps a high enzyme activity for a long time. Besides an inhibiting effect of the steroids produced in the interstitial cells, it is also possible that under normal circumstances an inhibiting substance is produced in the Sertoli cells, which is necessary for a harmonious balance between hypophysis and testes.

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Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). III. Esterases

Journal of Helminthology ◽

10.1017/s0022149x00006635 ◽

1980 ◽

Vol 54 (3) ◽

pp. 219-222 ◽

Cited By ~ 2

Author(s):

T. K. Roy

Keyword(s):

Enzyme Activity ◽

Vas Deferens ◽

Nonspecific Esterase ◽

Histochemical Methods ◽

Almost All ◽

Excretory Canal

ABSTRACTNonspecific esterase (NSE), acetylcholinesterase (AChE) and pseudocholinesterase (ChE) have been localized by histochemical methods in various tissues of a cestode, Raillietina (Raillietina) johri obtained from the intestine of pigeon.NSE has been found in the rostellum, suckers, hooks, tegument, subtegumental muscle, excretory canal, cirrus sac, vagina and eggs. Two types of cells have been recognized on the scolex surface—some are NSE positive and others are NSE negative. AChE, besides being localized in nerves, has also been visualized in almost all the structures as in case of NSE except in hooks, excretory canal and eggs. Additionally AChE has been observed in the vas deferens and sperm ductules. ChE has been observed only in nerves, vas deferens, cirrus sac and vagina; the intensity of enzyme activity being low when compared with AChE. Possible involvement of these enzymes in the physiology of the parasite has been discussed.

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Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111276 ◽

1984 ◽

Vol 42 ◽

pp. 232-233

Author(s):

S.M. Geyer ◽

C.L. Mendenhall ◽

J.T. Hung ◽

E.L. Cardell ◽

R.L. Drake ◽

...

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Enzyme Activity ◽

Malic Enzyme ◽

Smooth Endoplasmic Reticulum ◽

Rat Hepatocytes ◽

Mature Male ◽

Treatment Groups ◽

Malic Enzyme Activity ◽

Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

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