scholarly journals Fibrinogen ""New York"--an abnormal fibrinogen associated with thromboembolism: functional evaluation

Blood ◽  
1975 ◽  
Vol 45 (5) ◽  
pp. 607-619 ◽  
Author(s):  
H Al-Mondhiry ◽  
SB Bilezikian ◽  
HL Nossel
Keyword(s):  
New York ◽  
Fibrinogen Concentration ◽  
Functional Evaluation ◽  
Fibrinopeptide A ◽  
Life Threatening ◽  
History Of ◽  
And Control ◽  
Kinetics Of ◽  
Control Plasma

Abstract A 54-yr-old woman presented with a 23-yr history of repeated life- threatening thromboembolism. The presence of a qualitatively abnormal fibrinogen was suggested by the demonstration of delayed and incomplete coagulation of plasma or partially purified fibrinogen by thrombin or Reptilase. Two brothers showed a similar in vitro defect but were clinically not affected. The plasma fibrinogen concentration was 0.50- 1.64 mg/ml when estimated by heat turbidity, clottability, or immunologic techniques. The serum contained 80–820 mug/ml of unclottable fibrinogen-related materials even after 24 hr exposure to thrombin. The fibrinogen-related material in the serum showed faster anodal mobility an immunoelectrophoresis than that of normal plasma. Immunodiffusion studies with rabbit antihuman fibrinogen antiserum showed lines of identity between control plasma and the patient's plasma and serum. Studies of the kinetics of thrombin action on fibrinogen demonstrated impaired release of fibrinopeptide A and B and defective polymerization of preformed fibrin monomers. The maximum amount of fibrinopeptide A released by exhaustive treatment with thrombin was similar (per milligram protein) for both the patient's and control fibrinogen. This abnormal fibrinogen varient is tentatively designated fibrinogen “New York”; its possible identity with one of the previously described abnormal fibrinogens has not been excluded.

scholarly journals Fibrinogen ""New York"--an abnormal fibrinogen associated with thromboembolism: functional evaluation

Blood ◽  
1975 ◽  
Vol 45 (5) ◽  
pp. 607-619
Author(s):  
H Al-Mondhiry ◽  
SB Bilezikian ◽  
HL Nossel
Keyword(s):  
New York ◽  
Fibrinogen Concentration ◽  
Functional Evaluation ◽  
Fibrinopeptide A ◽  
Life Threatening ◽  
History Of ◽  
And Control ◽  
Kinetics Of ◽  
Control Plasma

A 54-yr-old woman presented with a 23-yr history of repeated life- threatening thromboembolism. The presence of a qualitatively abnormal fibrinogen was suggested by the demonstration of delayed and incomplete coagulation of plasma or partially purified fibrinogen by thrombin or Reptilase. Two brothers showed a similar in vitro defect but were clinically not affected. The plasma fibrinogen concentration was 0.50- 1.64 mg/ml when estimated by heat turbidity, clottability, or immunologic techniques. The serum contained 80–820 mug/ml of unclottable fibrinogen-related materials even after 24 hr exposure to thrombin. The fibrinogen-related material in the serum showed faster anodal mobility an immunoelectrophoresis than that of normal plasma. Immunodiffusion studies with rabbit antihuman fibrinogen antiserum showed lines of identity between control plasma and the patient's plasma and serum. Studies of the kinetics of thrombin action on fibrinogen demonstrated impaired release of fibrinopeptide A and B and defective polymerization of preformed fibrin monomers. The maximum amount of fibrinopeptide A released by exhaustive treatment with thrombin was similar (per milligram protein) for both the patient's and control fibrinogen. This abnormal fibrinogen varient is tentatively designated fibrinogen “New York”; its possible identity with one of the previously described abnormal fibrinogens has not been excluded.

Latex Hypersensitivity in Children: Clinical Presentation and Detection of Latex-Specific Immunoglobulin E

PEDIATRICS ◽  
1995 ◽  
Vol 95 (5) ◽  
pp. 693-699
Author(s):  
Pamela L. Kwittken ◽  
Sharon K. Sweinberg ◽  
Donald E. Campbell ◽  
Nicholas A. Pawlowski
Keyword(s):  
High Risk ◽  
Immunoglobulin E ◽  
High Risk Patient ◽  
Latex Allergy ◽  
Patient Profile ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Life Threatening ◽  
History Of

Objective. To better understand the clinical characteristics, diagnosis, and possible prevention of immediate hypersensitivity reactions to latex in a hospitalized, pediatric patient population. Methods. We performed a retrospective case analysis of the first 35 patients with latex allergy evaluated by our service over a 2-year period at our institution. Characteristics of patients and clinical reactions were analyzed and the presence of latex-specific immunoglobulin E was assessed using in vitro methods. In a limited group of Objective. To better understand the clinical premedication with steroids and antihistamines was evaluated for the prevention of latex allergic reactions. Results. The majority of our patients had life-threatening reactions. In previous reports, most pediatric patients underwent reactions in the perioperative period and belonged to two well-recognized "high-risk" patient groups (spina bifida and genitourinary malformations). In our series, 21 patients (60%) had reactions outside of the operating room setting, and 14 patients (40%) had primary diagnoses outside of the previously recognized "high-risk" groups. Many patients had a history of multiple surgical procedures, and a history of a surgical procedure in the first year of life was very common. A pre-existing clinical history of latex allergy was present in only 18 of the 35 patients, and a severe or life-threatening allergic reaction was the presenting feature of latex allergy in 11 of the 35 patients. Using in vitro assays, we were able to detect latex-specific immunoglobulin E in the sera of all but two of our patients. Latex gloves and latex-containing intravenous sets were common triggers for reactions. When exposure to latex occurs systemically, as through an intravenous line, premedication with steroids and antihistamines may fail to protect against anaphylaxis. Conclusions. Our experience indicates that the incidence of latex hypersensitivity in children is increasing, that the circumstances (patient profile, hospital location, route of exposure) in which life-threatening reactions may occur are more broad than previously reported, and that a better understanding of both environmental sources of latex antigens and host responses to latex exposure are needed for improved prevention of serious reactions.

scholarly journals Inspectors and Auditors: Some Sources of Andrey Platonov’s Work of the 1920s

2021 ◽  
Vol 6 (4) ◽  
pp. 274-299
Author(s):  
Maria V. Osipenko
Keyword(s):  
Communist Party ◽  
Real Life ◽  
Soviet State ◽  
Power And Control ◽  
Life Threatening ◽  
History Of ◽  
And Control

The article examines the work of Andrey Platonov of the 1920s in the context of the history of the Soviet state and Communist Party administration departments. Allusions to the activity of these authorities are present in many of Platonov’s works (“Gorod Gradov,” “Administrativnoe Estestvoznanie,” “Usomnivshiysya Makar,” “Nadlezhachie Meropriyatiya (Socialnaya Satira nashih dney),” etc.). Most of the references relate to anti-bureaucratic and rationalization activities of these power and control authorities. The effort to perfect bureaucratic machine is represented in Platonov’s work as pseudoproductive, pseudo-scientific, and meaningless activity. Yet, it is also shown how such pseudo-organization can become real life-threatening force.

Caution with bilateral salpingectomy and consideration of tubal stump ectopic in IVF

2020 ◽  
Vol 13 (11) ◽  
pp. e235893
Author(s):  
Swee Lin Yip ◽  
Shahul Hameed Mohamed Siraj ◽  
Jerry Kok Yen Chan
Keyword(s):  
Ectopic Pregnancy ◽  
Cystic Mass ◽  
In Vitro Fertilisation ◽  
Beta Human Chorionic Gonadotropin ◽  
Life Threatening ◽  
History Of ◽  
Bilateral Salpingectomy ◽  
Ectopic Pregnancies ◽  
The Right

We report a 35-year-old female patient with a history of bilateral salpingectomy from ectopic pregnancies presenting with a positive serum beta-human chorionic gonadotropin (bhCG) result following in vitro fertilisation (IVF) treatment. Apart from per vaginal spotting, she remained asymptomatic. Initial ultrasound showed an empty uterus with a cystic mass on the right side of the uterus. Serum beta-hCG was trended. A follow-up pelvic ultrasound 1 week later showed a live pregnancy in the right adnexa. A diagnostic laparoscopy was performed, which revealed an unruptured right stump ectopic pregnancy that was successfully removed. As a stump ectopic pregnancy can be a potentially life-threatening occurrence, we emphasise caution with salpingectomy and the consideration of tubal stump ectopic pregnancies following IVF treatment.

Development of Meningitis During Therapy with Cefamandole

PEDIATRICS ◽  
1981 ◽  
Vol 67 (5) ◽  
pp. 727-728
Author(s):  
S. C. Aronoff ◽  
W. Thomford ◽  
J. S. Bertino ◽  
W. T. Speck
Keyword(s):  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Pediatric Patients ◽  
Potential Hazard ◽  
Procaine Penicillin ◽  
Source Of Infection ◽  
Local Physician ◽  
Life Threatening ◽  
History Of

Cefamandole nafate is a relatively new cephalosporin approved for use in pediatric patients. This compound offers certain advantages over previously approved cephalosporins including its in vitro antimicrobial activity against β-lactamase-producing Haemophilus influenzae. The purpose of this report is to describe a potential hazard associated with the use of this and closely related antimicrobial agents in life-threatening bacterial infections. CASE REPORT This was the first Rainbow Babies and Childrens Hospital admission for this 7-month-old boy with a ten-day history of fever and irritability. Seven days prior to admission he was seen by a local physician for fever. No source of infection was noted and the infant was treated with 300,000 units of intramuscular procaine penicillin.

Myelopoiesis controlling agents in vitro

1964 ◽  
Vol 206 (3) ◽  
pp. 585-588 ◽  
Author(s):  
Julia Gidáli ◽  
Imre Fehér
Keyword(s):  
Bone Marrow ◽  
Initial Phase ◽  
Mitotic Rate ◽  
Characteristic Change ◽  
Normal Bone Marrow ◽  
Normal Bone ◽  
Rabbit Bone ◽  
And Control ◽  
Kinetics Of

The effect of sera from leukopenic and leukocytotic rabbits on normal rabbit bone marrow was studied in vitro. The rate of formate-C14 incorporation served for measuring the mitotic rate. Sera collected in the leukopenic phase or in the initial phase of leukocytosis, and incubated with normal bone marrow, were found to increase formate-C14 incorporation into cells significantly. On the other hand, sera collected at the peak of leukocytosis inhibited formate incorporation. Bone marrow from control rabbits incubated with sera from leukopenic and leukocytotic animals produced a characteristic change in the mitotic rate and in the ratio of myeloid precursors and mature myeloid elements. Kinetics of formate incorporation qualitatively agreed when bone marrow from leukopenic, leukocytotic, and control rabbits was incubated with the animals' own sera, or when bone marrow from control rabbits was incubated with leukopenic and leukocytotic sera, respectively. It may be assumed that the agents considered represent the humoral system maintaining the homeostasis of myeloid elements.

scholarly journals 1196. Serratia marcescens Strains Carrying blaKPC-2 and blaKPC-3 Carbapenemase Associated With Chronic Mechanical Ventilation

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S362-S362
Author(s):  
Matthew Fisher ◽  
Elizabeth Diago-Navarro ◽  
Olga Kaplun ◽  
Eric Sin ◽  
Bettina C Fries
Keyword(s):  
United States ◽  
New York ◽  
Serratia Marcescens ◽  
Tertiary Care ◽  
Pediatric Intensive Care ◽  
Carbapenem Resistance ◽  
The United States ◽  
Care Hospital ◽  
History Of

Abstract Background Carbapenem resistance (CR) in Enterobacteriaceae is a growing concern which the CDC has designated as an urgent threat. At our institution we have noted emergence of CR strains in clinical isolates including a growing number of Serratia marcescens. CR in Serratia marcescens in the United States is mostly reported to be encoded by the SME family of chromosomally encoded carbapenemases, while in Asia it has been described being mediated by transmissible plasmids such as KPC. Here we describe the emergence and characteristics of CR Serratia marcescens at an academic tertiary care hospital in New York. Methods Serratia marcescens isolates demonstrating in vitro carbapenem resistance were recovered over a 12-month period from six distinct patients. Antibiotic resistance was determined by standard methods. Real-time PCR for bla(KPC), mcr gene, bla(NDM-1), bla(VIM), and bla(OXA-48) was performed. Patient comorbidities, source of culture, location in the hospital, and co-infection with other CR organisms were investigated. Results Fourteen S. marcescens isolates demonstrating in vitro carbapenem resistance were recovered from six individual patients. All six patients had a history of chronic respiratory failure with tracheostomy and at least partial ventilator dependence. Five of the patients were located on the pulmonary intermediate care unit, and one in the pediatric intensive care unit. Twelve of the 14 isolates were tracheal or sputum cultures. Five of the sputum cultures from two patients were co-infected with CR Pseudomonas, and one sputum culture was simultaneously positive for CR Klebsiella pneumoniae and Enterobacter Cloacae. Nine out of 14 isolates were positive for blaKPC-3, two were blaKPC-2 positive, and three were blaKPC-negative, with no mechanism of carbapenem resistance determined yet. None of the other genes were detected. Conclusion Most carbapenem-resistant Serratia isolates were derived from respiratory tract and were found to be positive for blaKPC-3. This suggests that plasmid encoded carbapenemases are emerging among Serratia in the United States, which is already being reported in China. Genomic sequencing may establish whether this represents a clonal expansion and whether the blaKPC plasmid was transferred from Klebsiella or Enterobacter to Serratia in one of the patients. Disclosures All authors: No reported disclosures.

scholarly journals Placenta percreta after Strassman metroplasty of complete bicornuate uterus: a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chengyan Zhang ◽  
Xiaoxin Wang ◽  
Haili Jiang ◽  
Lei Hou ◽  
Liying Zou
Keyword(s):  
Reconstructive Surgical Procedures ◽  
Placenta Previa ◽  
Placenta Percreta ◽  
Reconstructive Procedures ◽  
Bicornuate Uterus ◽  
Severe Hemorrhage ◽  
Life Threatening ◽  
History Of ◽  
Ivf Et

Abstract Background A bicornuate uterus often results in infertility. While reconstructive procedures may facilitate pregnancy, spontaneous abortion or serious pregnancy complications may occur. We present a case of a bicornuate uterus with spontaneous conception after Strassman metroplasty; however, life-threatening complications during pregnancy occurred. Case presentation : A 38-year-old woman with a history of infertility presented for prenatal care at 6 weeks of gestation. She had conceived spontaneously after four failed in vitro fertilization and embryo transfer (IVF-ET) procedures, Strassman metroplasty for a complete bicornuate uterus, and two postoperative IVF-ET pregnancies that ended in embryo arrest. This pregnancy was uneventful until the patient presented with massive vaginal bleeding at 28 weeks of gestation and was diagnosed with placenta previa and placenta percreta. Bleeding was controlled after emergency Caesarean section and delivery of a healthy neonate. However, severe adhesions were noted as well as a rupture along the metroplasty scar. Two days later, on removal of the intrauterine gauze packing, severe hemorrhage resumed, and the uterus did not respond to oxytocin, hemabate, or carbetocin. Emergency hysterectomy was required. Conclusions Reconstructive surgical procedures for complete bicornuate uterus may allow patients to achieve spontaneous pregnancies. However, potential intrapartum complications include placenta implantation and postpartum hemorrhage, and the latter may be exacerbated as the uterus does not contract or respond to oxytocin or prostaglandin drugs. Patients should be counseled on the risks associated with pregnancy after Strassman metroplasty, and clinicians must be aware of potential severe complications.

scholarly journals Formulations and evaluation of Cyclodextrin complexed Ceadroxil loaded nanosponges

2017 ◽  
Vol 9 (3) ◽  
pp. 84 ◽  
Author(s):  
Pawan Dubey ◽  
Hemant Kumar Sharma ◽  
Sunil Shah ◽  
Chandra Kishore Tyagi ◽  
Amol R. Chandekar ◽  
...  
Keyword(s):  
Lactone Ring ◽  
Release Kinetics ◽  
Major Drawback ◽  
Zeta Potentials ◽  
Poorly Soluble ◽  
Dosing Intervals ◽  
And Control ◽  
Kinetics Of ◽  
Derivatives Of

<p>Cefadroxil (CFD) is a broad spectrum antibiotic that acts against an extensive variety of bacteria, including Gram-positive and Gram-negative bacteria. The major drawback of orally administered drug like cefadroxil is its shorter half life of 1.2 hrs. The goal of the study is to prolong the drug release, producing a desired blood serum level, reduction in drug toxicity and improving the patient compliance by prolonging the dosing intervals. Cyclodextrin-based nanosponges (NS) are a novel class of cross-linked derivatives of cyclodextrins. They have been used to increase the solubility of poorly soluble actives, to protect the labile groups and control the release. This study aimed at formulating complexes of CFDwith three types of β-cyclodextrin NS obtained with different cross-linking ratio (viz. 1:2, 1:4 and 1:8 on molar basis with the cross-linker) to protect the lactone ring from hydrolysis and to prolong the release kinetics of CFD. Crystalline (F<sub>1:2</sub>, F<sub>1:4</sub> and F<sub>1:8</sub>) and paracrystalline NS formulations were prepared. XRPD, DSC and FTIR studies confirmed the interactions of CFDwith NS. XRPD showed that the crystallinity of CFD decreased after loading. CFD was loaded as much as 21%, 37% and 13% w/w in F<sub>1:2 </sub>, F<sub>1:4</sub> and F<sub>1:8</sub>, respectively while the paracrystalline NS formulations gave a loading of about 10% w/w or lower. The particle sizes of the loaded NS formulations were between 450 and 600 nm with low polydispersity indices. The zeta potentials were sufficiently high (-20 to -25 mV) to obtain a stable colloidal nanosuspension. The in vitro studies indicated a slow and prolonged CFD release over a period of 24 h. The NS formulations protected the lactone ring of CFD after their incubation in physiological conditions at 37°C for 24 h with a 80% w/w of intact lactone ring when compared to only around 20% w/w of plain CFD.</p>

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