scholarly journals In vitro cytotoxic response to human myeloma plasma cells by peripheral blood leukocytes from patients with multiple myeloma and benign monoclonal gammopathy

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 226-237 ◽  
Author(s):  
T Paglieroni ◽  
MR MacKenzie
Keyword(s):  
Multiple Myeloma ◽  
Peripheral Blood ◽  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Cytotoxic Response ◽  
Normal Individuals ◽  
Benign Monoclonal Gammopathy

Abstract Peripheral blood leukocytes (PBL) from myeloma patients were studied for their capacity to lyse plasma cells from myeloma patients, benign monoclonal gammopathy (BMG) patients, and nonneoplastic disease patients. Plasma cells were isolated from bone marrow, labeled with 51Cr, and cultured with PBL isolated from patients with myeloma, BMG, or nonneoplastic disease, as well as normal individuals. PBL from patients with multiple myeloma demonstrated responses to autologous or allogeneic myeloma plasma cells. Optimum conditions for cytotoxic response included a responder-to-stimulator ratio of 1:1 and an effector-to-target ratio of 20:1. PBL from normal individuals or patients with BMG failed to demonstrate this response. However, PBL from BMG patients, but not normal individuals, could be induced to kill myeloma plasma cells (but not nonmyeloma plasma cells) by simultaneous stimulation with allogeneic lymphocytes and myeloma plasma cells.

scholarly journals In vitro cytotoxic response to human myeloma plasma cells by peripheral blood leukocytes from patients with multiple myeloma and benign monoclonal gammopathy

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 226-237
Author(s):  
T Paglieroni ◽  
MR MacKenzie
Keyword(s):  
Multiple Myeloma ◽  
Peripheral Blood ◽  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Cytotoxic Response ◽  
Normal Individuals ◽  
Benign Monoclonal Gammopathy

Peripheral blood leukocytes (PBL) from myeloma patients were studied for their capacity to lyse plasma cells from myeloma patients, benign monoclonal gammopathy (BMG) patients, and nonneoplastic disease patients. Plasma cells were isolated from bone marrow, labeled with 51Cr, and cultured with PBL isolated from patients with myeloma, BMG, or nonneoplastic disease, as well as normal individuals. PBL from patients with multiple myeloma demonstrated responses to autologous or allogeneic myeloma plasma cells. Optimum conditions for cytotoxic response included a responder-to-stimulator ratio of 1:1 and an effector-to-target ratio of 20:1. PBL from normal individuals or patients with BMG failed to demonstrate this response. However, PBL from BMG patients, but not normal individuals, could be induced to kill myeloma plasma cells (but not nonmyeloma plasma cells) by simultaneous stimulation with allogeneic lymphocytes and myeloma plasma cells.

scholarly journals Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro

2017 ◽  
Vol 51 (2) ◽  
pp. 39-45
Author(s):  
Milena Jankovic ◽  
Lada Zivkovic ◽  
Andrea Pirkovic ◽  
Dijana Topalovic ◽  
Dragana Dekanski ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Salvianolic Acid B ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Salvianolic Acid

scholarly journals Immune reactions in human filariasis

1978 ◽  
Vol 8 (2) ◽  
pp. 228-232 ◽  
Author(s):  
D Subrahmanyam ◽  
K Mehta ◽  
D S Nelson ◽  
Y V Rao ◽  
C K Rao
Keyword(s):  
Immunoglobulin G ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Wuchereria Bancrofti ◽  
Normal Subjects ◽  
Peripheral Blood Leukocytes ◽  
51Cr Release ◽  
Blood Leukocytes ◽  
Release Studies

Sera from cases of elephantiasis due to Wuchereria bancrofti infection promoted an intense adhesion of peripheral blood leukocytes to W. bancrofti microfilariae in vitro. A similar adhesion was also seen using sera from some normal persons living for several years in areas where filariasis is endemic. No such adhesion was evident with sera from microfilaria carriers or from normal subjects from nonendemic areas. The adhesion was complement independent and was associated with the immunoglobulin G fraction of serum. 51Cr release studies suggested the occurrence of cell-mediated cytotoxicity to W. bancrofti microfilariae in the presence of elephantiasis serum. Microfilariae of Litomosoides carinii could be isolated free of blood cells, from the blood of infected rats. In the presence of serum, or its immunoglobulin G fraction, from patients with elephantiasis, L. carinii microfilariae adhered to human peripheral blood leukocytes or rat spleen cells.

scholarly journals Differential Infectivity and Division ofToxoplasma gondii in Human Peripheral Blood Leukocytes

2000 ◽  
Vol 68 (8) ◽  
pp. 4822-4826 ◽  
Author(s):  
Jacqueline Y. Channon ◽  
Rosanne M. Seguin ◽  
Lloyd H. Kasper
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Differential Infectivity

ABSTRACT When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.

scholarly journals The Remission and Relapse Status of Acute Leukemia as Studied by the In Vitro Uptake of Tritiated Thymidine by Peripheral Blood Leukocytes

Blood ◽  
1968 ◽  
Vol 32 (2) ◽  
pp. 292-304
Author(s):  
THEODORE S. ZIMMERMAN ◽  
HERMAN A. GODWIN ◽  
MARVIN ZELEN ◽  
SEYMOUR PERRY
Keyword(s):  
Acute Leukemia ◽  
Peripheral Blood ◽  
Normal Population ◽  
Tritiated Thymidine ◽  
Additional Parameter ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Controls ◽  
In Vitro Uptake

Abstract H3TdR uptake by peripheral blood leukocytes has been measured in patients with acute leukemia and in hematologically normal controls. Patients with acute leukemia in remission and relapse generally had elevated uptakes and formed populations distinct from the normal population and from each other, although overlap of values between each population was present. The measurement of H3TdR uptake may prove useful as an additional parameter for studying acute leukemia.

scholarly journals The effects of tumor-promoting phorbol esters on human granulopoiesis in vitro

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 526-533 ◽  
Author(s):  
R Sullivan ◽  
RA Brodie ◽  
NE Larsen ◽  
PJ Gans ◽  
LA McCarroll
Keyword(s):  
Peripheral Blood ◽  
Colony Formation ◽  
Phorbol Esters ◽  
Marrow Cell ◽  
Adherent Cells ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Light Density ◽  
Marrow Cells

Abstract In order to determine whether the tumor-promoting phorbol esters are capable of inducing normal human committed granulocytic-monocytic progenitor cells (CFUc) to proliferate and differentiate in the absence of granulocyte-monocyte colony-stimulating activity (CSA), we studied the effects of these compounds on human granulopoiesis in vitro. We found that when light-density human marrow cells or peripheral blood leukocytes were depleted of adherent cells and then incubated in semisolid tissue culture medium under conditions optimal for CFUc growth, phorbol myristate acetate (PMA) and its congeners produced no measurable stimulatory effect on the proliferation of CFUc in the absence of added CSA. Likewise, when light-density marrow cells that had not been depleted of adherent cells were plated in the cultures, no stimulation of CFUc colony growth resulted from the addition of PMA. However, when light-density peripheral blood leukocytes were used as a target source of CFUc without first subjecting them to adherence separation, enhanced proliferation and differentiation of CFUc were noted in cultures that contained PMA. To investigate the possibility that CSA production by monocytes in these cultures in response to activation by PMA might account for the enhanced colony formation that we observed, we incubated isolated peripheral blood monocytes in short- term liquid suspension cultures and found that in the presence of PMA, large quantities of CSA were secreted into the surrounding medium. Finally, we noted that when marrow cell suspensions were suboptimally stimulated by low concentrations of CSA added to the cultures, the effects of PMA on CFUc proliferation were unpredictable, enhancing colony formation in some cases and inhibiting it in others. Our data indicate that although the tumor-promoting phorbol esters do not appear capable of directly stimulating the proliferation or differentiation of human CFUc in the absence of CSA, they may do so indirectly by causing auxiliary cells such as monocytes to secrete CSA.

In vitro interferon gamma regulation of CCR-3 mRNA expression in peripheral blood leukocytes from atopic asthmatics

2001 ◽  
Vol 1 (2) ◽  
pp. 75-80 ◽  
Author(s):  
I. Bello ◽  
M. R. Rizo ◽  
C. B. Badel ◽  
E. Blanco ◽  
C. Valenzuela ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Interferon Gamma ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes
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