Regulation of erythropoietin production in a human hepatoblastoma cell line

Production of immuno and biologically active erythropoietin was documented to occur in the human hepatoblastoma cell line HepG-2. The expression of the erythropoietin gene was further verified by Northern blot analysis using a single stranded RNA probe. In vitro studies showed that erythropoietin production by these cells was not stimulated by hypoxia or cobalt chloride, but was related to the proliferative activity of the cells in culture. In addition it was found that the secretion of erythropoietin was almost completely abrogated by tunicamycin, an inhibitor of N-linked glycosylation. This effect of tunicamycin was also observed in a permanently transfected cell line that secretes erythropoietin in large quantities.

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Regulation of erythropoietin production in a human hepatoblastoma cell line

Blood ◽

10.1182/blood.v70.6.1904.1904 ◽

1987 ◽

Vol 70 (6) ◽

pp. 1904-1909 ◽

Cited By ~ 37

Author(s):

OJ Nielsen ◽

SJ Schuster ◽

R Kaufman ◽

AJ Erslev ◽

J Caro

Keyword(s):

Cell Line ◽

Northern Blot ◽

Proliferative Activity ◽

Northern Blot Analysis ◽

Cobalt Chloride ◽

Biologically Active ◽

Erythropoietin Production ◽

Rna Probe ◽

Hepatoblastoma Cell Line

Abstract Production of immuno and biologically active erythropoietin was documented to occur in the human hepatoblastoma cell line HepG-2. The expression of the erythropoietin gene was further verified by Northern blot analysis using a single stranded RNA probe. In vitro studies showed that erythropoietin production by these cells was not stimulated by hypoxia or cobalt chloride, but was related to the proliferative activity of the cells in culture. In addition it was found that the secretion of erythropoietin was almost completely abrogated by tunicamycin, an inhibitor of N-linked glycosylation. This effect of tunicamycin was also observed in a permanently transfected cell line that secretes erythropoietin in large quantities.

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Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran

BMC Chemistry ◽

10.1186/s13065-021-00759-w ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Fariba Heshmati Afshar ◽

Masumeh Zadehkamand ◽

Zahra Rezaei ◽

Abbas Delazar ◽

Vahideh Tarhriz ◽

...

Keyword(s):

Cell Line ◽

Proliferative Activity ◽

Methanolic Extract ◽

Biologically Active ◽

Flavonoid Glycosides ◽

Control Group ◽

Chemical Compositions ◽

Preparative Hplc ◽

Cytotoxicity Activity ◽

Methanolic Extracts

Abstract Background Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by 1H and 13C NMR. Results The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. Conclusion Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.

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Erythropoietin production by a human testicular germ cell line

Blood ◽

10.1182/blood.v62.5.1132.1132 ◽

1983 ◽

Vol 62 (5) ◽

pp. 1132-1134 ◽

Cited By ~ 12

Author(s):

JL Ascensao ◽

F Gaylis ◽

D Bronson ◽

EE Fraley ◽

ED Zanjani

Keyword(s):

Germ Cell ◽

Cell Line ◽

Continuous Cell Line ◽

Human Testis ◽

Testicular Germ Cell ◽

Germ Cell Line ◽

Erythropoietin Production ◽

Unique Model

Abstract An established human testis germ cell line (1411-H) was found to produce significant amounts of erythropoietin (Ep), the primary regulator of erythropoiesis. Media conditioned by the 1411-H cells stimulated erythropoiesis both in vivo and in vitro. This activity was neutralized by anti-Ep. This continuous cell line provides a unique model for the study of the mechanisms of control of Ep biogenesis.

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Are Compounds Membrane-Associated or Present in the Cytosol? A Study Using Polyphenols in a Colon Carcinoma Cell Line Model

Current Pharmacology Reports ◽

10.1007/s40495-020-00242-5 ◽

2020 ◽

Vol 6 (6) ◽

pp. 451-456

Author(s):

Hannah Deusser ◽

Isabel Groh ◽

Tamara Bakuradze ◽

Nadine Simson ◽

Eva Kaiser ◽

...

Keyword(s):

Cell Membrane ◽

Cell Line ◽

Colon Carcinoma ◽

Carcinoma Cell ◽

Carcinoma Cell Line ◽

Biologically Active ◽

Colon Carcinoma Cell Line ◽

Cell Line Model ◽

Apple Products

Abstract Purpose We investigated the cytosolic and membrane-associated contents of polyphenols after 4 hours of incubation (50 μM of each polyphenol) in the colon carcinoma cell line T84 using a novel, rapid, and convenient method based on permeabilization of the cell membrane using digitonin. The colon carcinoma cell line was used to investigate the intestinal uptake of polyphenols present in apple products. Recent Findings The results showed that hydroxycinnamic acids (caffeic and 5-caffeoylquinic acid) were only detected in the cytosolic fractions. In contrast, 0.3 to 8.2% of the initial concentrations (50 μM) of the flavonoids phloretin, quercetin, phloretin 2′-O-glucoside, and quercetin 3-O-rhamnoside were found in the membrane-associated fractions. In the cytosolic fractions, 0.2–2.9% of these compounds were detected, corresponding to 25 to 40% of the total cell-associated (cytosolic plus membrane-associated fractions) polyphenol content. Summary Our results showed that after uptake, polyphenols were present in the cytosolic fraction of the cells as well as associated with the cell membrane. The presented method provides a useful in vitro tool for determining biologically active compounds in cellular fractions.

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1,25-Dihydroxyvitamin D3 Induces Differentiation of a Retinoic Acid–Resistant Acute Promyelocytic Leukemia Cell Line (UF-1) Associated With Expression of p21WAF1/CIP1 and p27KIP1

Blood ◽

10.1182/blood.v93.7.2225.407a05_2225_2233 ◽

1999 ◽

Vol 93 (7) ◽

pp. 2225-2233 ◽

Cited By ~ 5

Author(s):

Akihiro Muto ◽

Masahiro Kizaki ◽

Kenji Yamato ◽

Yohko Kawai ◽

Maiko Kamata-Matsushita ◽

...

Keyword(s):

Retinoic Acid ◽

Cell Line ◽

Acute Promyelocytic Leukemia ◽

Northern Blot ◽

Blot Analysis ◽

Northern Blot Analysis ◽

Promyelocytic Leukemia ◽

G1 Arrest ◽

Dihydroxyvitamin D3 ◽

1,25 Dihydroxyvitamin D3

Retinoic acid (RA) resistance is a serious problem for patients with acute promyelocytic leukemia (APL) who are receiving all-transRA. However, the mechanisms and strategies to overcome RA resistance by APL cells are still unclear. The biologic effects of RA are mediated by two distinct families of transcriptional factors: RA receptors (RARs) and retinoid X receptors (RXRs). RXRs heterodimerize with 1,25-dihydroxyvitamin D3[1,25(OH)2D3] receptor (VDR), enabling their efficient transcriptional activation. The cyclin-dependent kinase (cdk) inhibitor p21WAF1/CIP1 has a vitamin D3–responsive element (VDRE) in its promoter, and 1,25(OH)2D3 enhances the expression of p21WAF1/CIP1 and induces differentiation of selected myeloid leukemic cell lines. We have recently established a novel APL cell line (UF-1) with features of RA resistance. 1,25(OH)2D3 can induce growth inhibition and G1 arrest of UF-1 cells, resulting in differentiation of these cells toward granulocytes. This 1,25(OH)2D3-induced G1 arrest is enhanced by all-trans RA. Also, 1,25(OH)2D3 (10−10 to 10−7 mol/L) in combination with RA markedly inhibits cellular proliferation in a dose- and time-dependent manner. Associated with these findings, the levels of p21WAF1/CIP1 and p27KIP1 mRNA and protein increased in these cells. Northern blot analysis showed that p21WAF1/CIP1 and p27KIP1 mRNA and protein increased in these cells. Northern blot analysis showed that p21WAF1/CIP1 and p27KIP1 transcripts were induced after 6 hours’ exposure to 1,25(OH)2D3 and then decreased to basal levels over 48 hours. Western blot experiments showed that p21WAF1/CIP1 protein levels increased and became detectable after 12 hours of 1,25(OH)2D3treatment and induction of p27KIP1 protein was much more gradual and sustained in UF-1 cells. Interestingly, the combination of 1,25(OH)2D3 and RA markedly enhanced the levels of p27KIP1 transcript and protein as compared with levels induced by 1,25(OH)2D3 alone. In addition, exogenous p27KIP1 expression can enhance the level of CD11b antigen in myeloid leukemic cells. In contrast, RA alone can induce G1 arrest of UF-1 cells; however, it did not result in an increase of p21WAF1/CIP1 and p27KIP1transcript and protein expression in RA-resistant cells. Taken together, we conclude that 1,25(OH)2D3 induces increased expression of cdk inhibitors, which mediates a G1 arrest, and this may be associated with differentiation of RA-resistant UF-1 cells toward mature granulocytes.

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