P-glycoprotein expression in plasma-cell myeloma is associated with resistance to VAD

Abstract Tumor cell-associated expression of multidrug resistance (MDR) was quantitated in 22 patients with DNA-aneuploid myeloma using 2-parameter flow cytometry with monoclonal antibody (MoAb) C-219 for the detection of cytoplasmic p-170 and propidium iodide for nuclear DNA content. The proportion of cells expressing p-170 and the intensity of p-170-related fluorescence were determined for each patient. Among the 14 patients treated with vincristine-adriamycin-dexamethasone (VAD), the proportion of p-170-positive cells distinguished sensitive from resistant disease (P less than .01). Among a subgroup of seven patients with MDR analysis available prior to VAD therapy, two subsequent nonresponders had high proportions of C-219-reactive cells. The presence de novo of high proportions of p-170-expressing cells in another still untreated patient and in a further individual with resistance to dexamethasone and interferon (not associated with MDR) warrants systematic analysis of p-170 expression prior to therapy to determine its clinical implications for response to MDR-associated drugs as combined in the VAD regimen. Concurrent MDR expression by aneuploid tumor cells and cells in the diploid subcompartment may represent involvement of diploid cells in the myeloma disease process.

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P-glycoprotein expression in plasma-cell myeloma is associated with resistance to VAD

Blood ◽

10.1182/blood.v74.3.913.bloodjournal743913 ◽

1989 ◽

Vol 74 (3) ◽

pp. 913-917 ◽

Cited By ~ 3

Author(s):

J Epstein ◽

HQ Xiao ◽

BK Oba

Keyword(s):

Nuclear Dna ◽

De Novo ◽

Disease Process ◽

Nuclear Dna Content ◽

Clinical Implications ◽

Systematic Analysis ◽

Resistant Disease ◽

P Glycoprotein ◽

Aneuploid Tumor ◽

Diploid Cells

Tumor cell-associated expression of multidrug resistance (MDR) was quantitated in 22 patients with DNA-aneuploid myeloma using 2-parameter flow cytometry with monoclonal antibody (MoAb) C-219 for the detection of cytoplasmic p-170 and propidium iodide for nuclear DNA content. The proportion of cells expressing p-170 and the intensity of p-170-related fluorescence were determined for each patient. Among the 14 patients treated with vincristine-adriamycin-dexamethasone (VAD), the proportion of p-170-positive cells distinguished sensitive from resistant disease (P less than .01). Among a subgroup of seven patients with MDR analysis available prior to VAD therapy, two subsequent nonresponders had high proportions of C-219-reactive cells. The presence de novo of high proportions of p-170-expressing cells in another still untreated patient and in a further individual with resistance to dexamethasone and interferon (not associated with MDR) warrants systematic analysis of p-170 expression prior to therapy to determine its clinical implications for response to MDR-associated drugs as combined in the VAD regimen. Concurrent MDR expression by aneuploid tumor cells and cells in the diploid subcompartment may represent involvement of diploid cells in the myeloma disease process.

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Correlated flow cytometric analysis of H-ras p21 and nuclear DNA in multiple myeloma

Blood ◽

10.1182/blood.v72.2.796.796 ◽

1988 ◽

Vol 72 (2) ◽

pp. 796-800 ◽

Cited By ~ 36

Author(s):

H Tsuchiya ◽

J Epstein ◽

P Selvanayagam ◽

JR Dedman ◽

G Gallick ◽

...

Keyword(s):

Multiple Myeloma ◽

Nuclear Dna ◽

Plasma Cells ◽

Bone Marrow Cells ◽

Flow Cytometric Analysis ◽

Nuclear Dna Content ◽

Normal Donor ◽

Aneuploid Tumor ◽

Ras P21 ◽

Diploid Cells

Abstract Correlated analysis of the H-ras oncogenes product (p21) and of nuclear DNA content was performed by flow cytometry (FCM) in patients with DNA- aneuploid multiple myeloma (MM). Bone marrow cells from normal donors and MM patients in remission served as controls. Seventy-four percent of 23 patients with active MM had higher p21 fluorescence in aneuploid tumor cells than were observed in normal donor or myeloma remission bone marrows; 39% of the 23 patients also showed high H-ras p21 expression in diploid cells. There was an inverse relationship between p21 levels and the presence of trisomy 11; especially high p21 levels were noted in patient without trisomy 11. The frequent elevation of p21 protein in aneuploid plasma cells suggests the involvement of the H-ras oncogene in the pathophysiology of MM, which is further supported by a shorter survival among patients with high p21 levels.

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Correlated flow cytometric analysis of H-ras p21 and nuclear DNA in multiple myeloma

Blood ◽

10.1182/blood.v72.2.796.bloodjournal722796 ◽

1988 ◽

Vol 72 (2) ◽

pp. 796-800 ◽

Cited By ~ 2

Author(s):

H Tsuchiya ◽

J Epstein ◽

P Selvanayagam ◽

JR Dedman ◽

G Gallick ◽

...

Keyword(s):

Multiple Myeloma ◽

Nuclear Dna ◽

Plasma Cells ◽

Bone Marrow Cells ◽

Flow Cytometric Analysis ◽

Nuclear Dna Content ◽

Normal Donor ◽

Aneuploid Tumor ◽

Ras P21 ◽

Diploid Cells

Correlated analysis of the H-ras oncogenes product (p21) and of nuclear DNA content was performed by flow cytometry (FCM) in patients with DNA- aneuploid multiple myeloma (MM). Bone marrow cells from normal donors and MM patients in remission served as controls. Seventy-four percent of 23 patients with active MM had higher p21 fluorescence in aneuploid tumor cells than were observed in normal donor or myeloma remission bone marrows; 39% of the 23 patients also showed high H-ras p21 expression in diploid cells. There was an inverse relationship between p21 levels and the presence of trisomy 11; especially high p21 levels were noted in patient without trisomy 11. The frequent elevation of p21 protein in aneuploid plasma cells suggests the involvement of the H-ras oncogene in the pathophysiology of MM, which is further supported by a shorter survival among patients with high p21 levels.

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P-glycoprotein, lung resistance-related protein and multidrug resistance associated protein in de novo acute non-lymphocytic leukaemias: biological and clinical implications

British Journal of Haematology ◽

10.1046/j.1365-2141.1999.01172.x ◽

1999 ◽

Vol 104 (2) ◽

pp. 328-335 ◽

Cited By ~ 86

Author(s):

Mariagrazia Michieli ◽

Daniela Damiani ◽

Anna Ermacora ◽

Paola Masolini ◽

Donatella Raspadori ◽

...

Keyword(s):

Multidrug Resistance ◽

De Novo ◽

Clinical Implications ◽

Related Protein ◽

P Glycoprotein ◽

Lung Resistance

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Genome size and identification of abundant repetitive sequences in Vallisneria spinulosa

PeerJ ◽

10.7717/peerj.3982 ◽

2017 ◽

Vol 5 ◽

pp. e3982 ◽

Cited By ~ 3

Author(s):

RuiJuan Feng ◽

Xin Wang ◽

Min Tao ◽

Guanchao Du ◽

Qishuo Wang

Keyword(s):

Genome Size ◽

Aquatic Plant ◽

Nuclear Dna ◽

De Novo ◽

Repetitive Sequences ◽

Nuclear Dna Content ◽

Ltr Retrotransposons ◽

Sequencing Data ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

Vallisneria spinulosa is a freshwater aquatic plant of ecological and economic importance. However, there is limited cytogenetic and genomics information on Vallisneria. In this study, we measured the nuclear DNA content of Vallisneria spinulosa by flow cytometry, performed a de novo assembly, and annotated repetitive sequences by using a combination of next-generation sequencing (NGS) and bioinformatics tools. The genome size of Vallisneria spinulosa is approximately 3,595 Mbp, in which nearly 60% of the genome consists of repetitive sequences. The majority of the repetitive sequences are LTR-retrotransposons comprising 43% of the genome. Although the amount of sequencing data used in this study was not sufficient for a whole-genome assembly, it could generate an overview of representative elements in the genome. These results will lay a new foundation for further studies on various species that belong to the Vallisneria genus.

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Nuclear DNA content and ultrastructure of secretory cells of Vicia faba L. stigma

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1994.018 ◽

2014 ◽

Vol 63 (2) ◽

pp. 139-145 ◽

Cited By ~ 2

Author(s):

Bogdan Wróbel ◽

Elżbieta Bednarska

Keyword(s):

Vicia Faba ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Development Stage ◽

Secretory Cells ◽

Vicia Faba L ◽

Three Stages ◽

Object Of Study ◽

Diploid Cells ◽

Vacuolar System

The object of study was the level of nuclear DNA and the ultrastructural transformations in the secretory cells of the stigma in <i>Vicia faba</i> L. It has been found that the stigmal cells which are active in biogenesis and exudate secretion are diploid cells whose differentiation starts from 2C DNA level. The presence of a population of nuclei with an amount DNA of about 2.5 C suggests that the metabolic activity of those cells may be regulated through supplementary incomplete replication. The ultrastructural transformations of secretory cells point to three stages of biogenesis and secretion of exudate. Stage I, before the start of the cell's secretory functions, is characterized by the development of the protein synthesizing apparatus and the activity of dictyosomes. In development stage II vesicular electron-transparent exudate is secreted. Stage III of exudate biogenesis is production of lipids. They form mainly in the plastids and are secreted with the involvement of the cell's vacuolar system.

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Study of chromosomes, DNA amount, and in vitro growth in different species of Luzula

Genome ◽

10.1139/g90-022 ◽

1990 ◽

Vol 33 (1) ◽

pp. 143-147 ◽

Cited By ~ 4

Author(s):

Jayanti Sen ◽

Sumona Mukherjee ◽

A. K. Sharma

Keyword(s):

Chromosome Number ◽

Dna Content ◽

Nuclear Dna ◽

Marked Change ◽

Nuclear Dna Content ◽

In Vitro Growth ◽

Dna Amount ◽

Diploid Cells ◽

In Cells

Cells of six different species of Luzula were subjected to in vitro culture and examined for callus formation. The cells of calli from the different species were analyzed for variation in chromosome number. The cells of different species showed a differential response in culture with respect to callus growth. Chromosome number variation in vitro, including both hypo- and hyper-diploidy, was recorded in all species except L. pediformis and L. luzuloides. The chromosome fragments survived as a result of a nonlocalized centromere. With prolonged culture, normal diploid cells were frequent. The nuclear DNA content of cells of these species was also measured, both in vivo and in vitro. The DNA values ranged from 6.05 to 7.03 pg per 4C nucleus. No marked change in DNA value was noted in cells even with high chromosome numbers, thereby confirming their origin through fragmentation of chromosomes. In cells of callus, the DNA amount was observed to be higher than in normal cells, indicating fragmentation and duplication of individual chromosomes. Their importance in the origin of new genotypes has been indicated.Key words: Luzula spp., in vitro growth, chromosomes, nuclear DNA content.

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PrimPol (Primase/Polymerase), replicating enzyme – A mini review

Pak-Euro Journal of Medical and Life Sciences ◽

10.31580/pjmls.v2i4.956 ◽

2020 ◽

Vol 2 (4) ◽

pp. 89-92

Author(s):

Muhammad Amir ◽

Sabeera Afzal ◽

Alia Ishaq

Keyword(s):

Dna Replication ◽

Dna Polymerase ◽

Genetic Information ◽

Nuclear Dna ◽

De Novo ◽

Dna Polymerases ◽

Test Site ◽

Mitochondrial Dna Replication ◽

Dna Template ◽

Preliminary Phase

Polymerases were revealed first in 1970s. Most important to the modest perception the enzyme responsible for nuclear DNA replication that was pol , for DNA repair pol and for mitochondrial DNA replication pol DNA construction and renovation done by DNA polymerases, so directing both the constancy and discrepancy of genetic information. Replication of genome initiate with DNA template-dependent fusion of small primers of RNA. This preliminary phase in replication of DNA demarcated as de novo primer synthesis which is catalyzed by specified polymerases known as primases. Sixteen diverse DNA-synthesizing enzymes about human perspective are devoted to replication, reparation, mutilation lenience, and inconsistency of nuclear DNA. But in dissimilarity, merely one DNA polymerase has been called in mitochondria. It has been suggest that PrimPol is extremely acting the roles by re-priming DNA replication in mitochondria to permit an effective and appropriate way replication to be accomplished. Investigations from a numeral of test site have significantly amplified our appreciative of the role, recruitment and regulation of the enzyme during DNA replication. Though, we are simply just start to increase in value the versatile roles that play PrimPol in eukaryote.

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