Effect of echinochrome on the respiratory burst of blood polymorphonuclear neutrophils in pediatric patients with interstitial lung disease (ILD): an in vitro study

Arecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy subjects and chronic periodontitis (CP) patients. An in vitro study was designed with an equal number of (n = 30) gingival crevicular fluid (GCF) samples collected from CP patients and healthy subjects. Bactericidal activity and hydrogen peroxide (H2O2) assays were performed with the GCF samples pre-treated with extracts of two varieties of areca nut: ripe and tender. Simultaneously, controls were also carried out with Hank’s balanced salt solution (HBSS) and catechin. Independent t-test and one-way analysis of variance (ANOVA), along with post-hoc analysis, were employed for statistical analysis. In both study groups, a significant reduction (p < 0.01)in the bactericidal activity was noted when the samples treated with the ripe areca nut (rANE) were compared with the tender variant (tANE). Similarly, H2O2 levels were significantly reduced (p < 0.001) in the rANE in contrast to tANE for both study groups. The above results were significant within the group but were found to be non-significant between the study groups, except when it was treated with HBSS (p < 0.001). In the present study, it was found that there was a reduction in the bactericidal activity and H2O2 production of cPMNs in both healthy subjects and CP patients in the presence of areca nut extract. Moreover, the effect of rANE on cPMNs was more detrimental than tANE.

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A comparison of asparaginase activity in generic formulations of E.coli derived L‐ asparaginase: In‐vitro study and retrospective analysis of asparaginase monitoring in pediatric patients with leukemia

British Journal of Clinical Pharmacology ◽

10.1111/bcp.14216 ◽

2020 ◽

Vol 86 (6) ◽

pp. 1081-1088 ◽

Cited By ~ 1

Author(s):

Hari Sankaran ◽

Soumika Sengupta ◽

Vaitashi Purohit ◽

Anand Kotagere ◽

Nirmalya Roy Moulik ◽

...

Keyword(s):

Retrospective Analysis ◽

Pediatric Patients ◽

In Vitro Study ◽

Vitro Study ◽

Asparaginase Activity

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STAT3 polymorphism associates with mTOR inhibitor-induced interstitial lung disease in patients with renal cell carcinoma

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics ◽

10.3727/096504022x16418911579334 ◽

2022 ◽

Author(s):

Kazuhiro Yamamoto ◽

Takeshi Ioroi ◽

Kazuaki Shinomiya ◽

Ayaka Yoshida ◽

Kenichi Harada ◽

...

Keyword(s):

Renal Cell Carcinoma ◽

Interstitial Lung Disease ◽

Lung Disease ◽

Cell Carcinoma ◽

Cell Lines ◽

Renal Cell ◽

Mtor Inhibitor ◽

In Vitro Study ◽

Primary Analysis

We evaluated the association of signal transducer and activator of transcription 3(STAT3) polymorphisms with the incidence of mammalian target of rapamycin (mTOR) inhibitor-induced interstitial lung disease (ILD) in patients with renal cell carcinoma (RCC). We also used lung-derived cell lines to investigate the mechanisms of this association. Japanese patients with metastatic RCC who were treated with mTORinhibitors were genotyped for the STAT3 polymorphism, rs4796793. We evaluated the association of the STAT3 genotype with the incidence of ILD and therapeutic outcome.In the 57 patients included in the primary analysis, the ILD rate within 140 days was significantly higher in patients with the CC genotype compared with those with other genotypes (77.8% versus 23.1%, odds ratio = 11.67, 95% confidential interval = 3.06–44.46). Meanwhile, there were no significant differences in progression-free survival ortime-to-treatment failure between the patients with the CC genotype and those with other genotypes. An in vitro study demonstrated that some lung-derived cell lines carrying the CC genotype exhibited an increase in the expression of mesenchymal markers, such as fibronectin, N-cadherin, and vimentin and decreases in E-cadherin, which is an epithelial marker associated with exposure to everolimus, although STAT3 expression and activity were not related to the genotype. In conclusion, the CCgenotype of the STAT3 rs4796793 polymorphism increases the risk of mTOR inhibitorinduced ILD, supporting its use as a predictive marker for RCC.

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