Global gene expression in endometrium of high and low fertility heifers during the mid-luteal phase of the estrous cycle

Embryonic mortality is a major constraint to improving reproductive efficiency and profitability in livestock enterprises. We previously reported differential expression of genes with identified roles in cellular growth and proliferation, lipid metabolism, endometrial remodeling, inflammation, angiogenesis, and metabolic exchange in endometrial tissue on day 7 of the estrous cycle (D7), between heifers ranked as either high (HF) or low (LF) for fertility. The aim of the current study was to further elucidate the underlying molecular mechanisms contributing to early embryo loss by examining differential endometrial gene expression in HF or LF heifers at a later stage of the estrous cycle; day 14 (D14). A second objective was to compare these expression profiles with those from midluteal HF and LF endometrium. Using the same animal model as employed in the previous study, we slaughtered HF and LF animals on D14, harvested endometrial tissue, and carried out global gene expression analysis using the Affymetrix Bovine GeneChip. Microarray analysis detected 430 differentially expressed genes (DEG) between HF and LF animals. Ingenuity Pathway Analysis revealed enrichment for a host of biological pathways including lipid metabolism, molecular transport, immune response, cell morphology and development, and cell growth and proliferation. Important DEG included ALB, BMPR2, CCL28, COL4A3/4, FADS1, ITGA6, LDLR, PLCB3, PPARG, PTGS2, and SLC27A4. Furthermore, DEG expressed on both D7 and D14 included: PCCB, SLC25A24, DAP, and COL4A4. This study highlights some of the pathways and mechanisms underpinning late luteal bovine endometrial physiology and endometrial-related conception rate variance.

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Uterine endometrial gene expression in heifers of high or low fertility on day 14 of the estrous cycle

Advances in Animal Biosciences ◽

10.1017/s2040470010003043 ◽

2010 ◽

Vol 1 (1) ◽

pp. 161-161

Author(s):

AP Killeen ◽

DG Morris ◽

DA Kenny ◽

MG Diskin ◽

SM Waters

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Low Fertility

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Comparison of expression patterns of six canonical clock genes of follicular phase and luteal phase in Small-tailed Han sheep

Archives Animal Breeding ◽

10.5194/aab-64-457-2021 ◽

2021 ◽

Vol 64 (2) ◽

pp. 457-466

Author(s):

Qi Han ◽

Xiaoyun He ◽

Ran Di ◽

Mingxing Chu

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Luteal Phase ◽

Clock Gene ◽

Clock Genes ◽

Expression Patterns ◽

Mrna Levels ◽

Clock Gene Expression ◽

The Relationship ◽

The Brain

Abstract. The circadian rhythm is a biological rhythm that is closely related to the rhythmic expression of a series of clock genes. Results from several studies have indicated that clock genes are associated with the estrous cycle in female animals. Until now, the relationship between estrus cycle transition and clock gene expression in reproductive-axis-related tissues has remained unknown in Small-tailed Han (STH) sheep. This study was conducted to analyze the expression patterns of six canonical clock genes (Clock, BMAL1, Per1, Per2, Cry1, and Cry2) in the follicle phase and luteal phase of STH sheep. We found that all six genes were expressed in the brain, cerebellum, hypothalamus, pituitary, ovary, uterus, and oviduct in follicle and luteal phases. The results indicated that Clock expression was significantly higher in the cerebellum, hypothalamus, and uterus of the luteal phase than that of the follicle phase, whereas BMAL1 expression was significantly higher in the hypothalamus of the luteal phase than that of the follicle phase. Per1 expression was significantly higher in the brain, cerebellum, hypothalamus, and pituitary of the luteal phase than that of the follicle phase, and Per2 expression was significantly higher in the hypothalamus, pituitary, and uterus of the luteal phase than that of the follicle phase. Cry1 expression was significantly higher in the brain, cerebellum, and hypothalamus of the luteal phase than that of the follicle phase, whereas Cry2 expression was significantly higher in the pituitary of the luteal phase than that of the follicle phase. The clock gene expression in all tissues was different between follicle and luteal phases, but all clock gene mRNA levels were found to exhibit higher expression among seven tissues in the luteal phase. Our results suggest that estrous cycles may be associated with clock gene expression in the STH sheep. This is the first study to systematically analyze the expression patterns of clock genes of different estrous cycle in ewes, which could form a basis for further studies to develop the relationship between clock genes and the estrous cycle.

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Exploration of Global Gene Expression Changes During the Estrous Cycle in Equine Endometrium1

Biology of Reproduction ◽

10.1095/biolreprod.112.103226 ◽

2012 ◽

Vol 87 (6) ◽

Cited By ~ 20

Author(s):

Simone Gebhardt ◽

Maximiliane Merkl ◽

Nadja Herbach ◽

Rüdiger Wanke ◽

Johannes Handler ◽

...

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Global Gene Expression

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Differential Gene Expression in Oviducts Collected During Proestrus from Ewes Fasted During the Luteal Phase of the Estrous Cycle.

Biology of Reproduction ◽

10.1093/biolreprod/78.s1.109a ◽

2008 ◽

Vol 78 (Suppl_1) ◽

pp. 109-109

Author(s):

Kathleen J. Austin ◽

Kristi M. Cammack ◽

Edward A. Van Kirk ◽

Gary E. Moss ◽

Brenda M. Alexander

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Differential Gene Expression ◽

Luteal Phase ◽

Differential Gene

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Differential gene expression profiling of endometrium during the mid-luteal phase of the estrous cycle between a repeat breeder (RB) and non-RB cows

Reproductive Biology and Endocrinology ◽

10.1186/s12958-017-0237-6 ◽

2017 ◽

Vol 15 (1) ◽

Cited By ~ 7

Author(s):

Ken-Go Hayashi ◽

Misa Hosoe ◽

Keiichiro Kizaki ◽

Shiori Fujii ◽

Hiroko Kanahara ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Estrous Cycle ◽

Differential Gene Expression ◽

Expression Profiling ◽

Luteal Phase ◽

Differential Gene ◽

Repeat Breeder

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Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

International Journal of Molecular Sciences ◽

10.3390/ijms21082777 ◽

2020 ◽

Vol 21 (8) ◽

pp. 2777

Author(s):

Damian Tanski ◽

Agnieszka Skowronska ◽

Maciej Eliszewski ◽

Leszek Gromadzinski ◽

Bartosz Kempisty ◽

...

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Luteal Phase ◽

Integral Membrane Proteins ◽

Early Cleavage ◽

Fluid Homeostasis ◽

Late Luteal Phase ◽

First Time ◽

Oviductal Fluid

Aquaporins (AQPs) are a group of small, integral membrane proteins which play an important role in fluid homeostasis in the reproductive system. In our previous study, we demonstrated AQP1, 5 and 9 protein expression and localization in the porcine oviduct. The presence of these isoforms could suggest their role in the transport of the ovum to the uterus by influencing the epithelial cells’ production of oviductal fluid. The aim of this study was to evaluate the expression of AQP1, AQP5 and AQP9 in the infundibulum, ampulla and isthmus in the porcine oviduct during the estrous cycle (early luteal phase, days 2–4, medium luteal phase, days 10–12, late luteal phase days 14–16, follicular phase days 18–20) and pregnancy (period before implantation, days 14–16 and after the implantation, days 30–32) using the Real-Time PCR technique. As clearly demonstrated for the first time, AQP1, 5, and 9 gene expression is influenced by the estrus cycle and pregnancy. Furthermore, expression of AQPs in the porcine oviduct may provide the physiological medium that sustains and enhances fertilization and early cleavage-stage embryonic development. Overall, our study provides a characterization of oviduct AQPs, increasing our understanding of fluid homeostasis in the porcine oviduct to successfully establish and maintain pregnancy.

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