Recombination analysis reveals a double recombination event in hepatitis E virus

RNA recombination is a significant driving force in viral evolution. Increased awareness of recombination within the genus Norovirus of the family Calicivirus has led to a rise in the identification of norovirus (NoV) recombinants and they are now reported at high frequency. Currently, there is no classification system for recombinant NoVs and a widely accepted recombinant genotyping system is still needed. Consequently, there is duplication in reporting of novel recombinants. This has led to difficulties in defining the number and types of recombinants in circulation. In this study, 120 NoV nucleotide sequences were compiled from the current GenBank database and published literature. NoV recombinants and their recombination breakpoints were identified using three methods: phylogenetic analysis, SimPlot analysis and the maximum χ 2 method. A total of 20 NoV recombinant types were identified in circulation worldwide. The recombination point is the ORF1/2 overlap in all isolates except one, which demonstrated a double recombination event within the polymerase region.

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A quadruple recombination event discovered in hepatitis E virus

Archives of Virology ◽

10.1007/s00705-021-05251-3 ◽

2021 ◽

Author(s):

Hao Shen ◽

Shuning Liu ◽

Min Ding ◽

Haixia Gu ◽

Ming Chang ◽

...

Keyword(s):

Recombination Event ◽

Hepatitis E Virus ◽

Hepatitis E

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Construction of an Unstable Ring-X Chromosome Bearing the Autosomal Dopa Decarboxylase Gene in Drosophila melanogaster and Analysis of Ddc Mosaics

Genetics ◽

10.1093/genetics/115.2.305 ◽

1987 ◽

Vol 115 (2) ◽

pp. 305-311

Author(s):

Donald A Gailey ◽

Deborah L Bordne ◽

Ana Maria Vallés ◽

Jeffrey C Hall ◽

Kalpana White

Keyword(s):

Nervous System ◽

Drosophila Melanogaster ◽

X Chromosome ◽

Recombination Event ◽

P Element ◽

Dopa Decarboxylase ◽

Somatic Instability ◽

Absolute Requirement ◽

Double Recombination ◽

Double Recombination Event

ABSTRACT An unstable Ring-X chromosome, Ddc +- Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed. The construction involved a double recombination event between the unstable Ring-X, R(1)wvC and a Rod-X chromosome which contained a P-element mediated Ddc + insert. The resulting Ddc +-Ring-X chromosome behaves similarly to the parent chromosome with respect to somatic instability. The Ddc +-Ring-X chromosome was used to generate Ddc mosaics. Analyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc + expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.

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Quadruple Recombination Events Discovered in Hepatitis E Virus

10.21203/rs.3.rs-594361/v1 ◽

2021 ◽

Author(s):

Hao Shen ◽

Shuning Liu ◽

Min Ding ◽

Haixia Gu ◽

Ming Chang ◽

...

Keyword(s):

Recombination Event ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Progeny Virus ◽

Future Research ◽

Genotype 1 ◽

Genotype 3 ◽

Minor Parent ◽

Detection Analysis ◽

Orf2 Region

Abstract Hepatitis E virus (HEV) can infect humans, pigs, and many other animals, but the recombination of HEV has rarely been reported. In the present study, phylogenetic and recombination analyses were performed on 557 complete HEV genomes in GenBank. A potentially significant quadruple recombination event was identified by recombination detection analysis. The recombinant progeny virus HEV_32_Manchester_301214 was produced by recombination between the major parent HEPAC-44 and the minor parent HE-JA15-1335, thereby reflecting inter-genotype recombination. HEV_32_Manchester_301214 and HEPAC-44 belong to genotype 3, while HE-JA15-1335 belongs to genotype 1, and these three strains have all been separated from humans. Three breakpoints of the four recombination events occurred in the ORF2 region, while another occurred in the ORF1 region. This quadruple recombination event was confirmed by phylogenetic analysis. The genotype, host and the recombination regions of the three strains were analyzed. These results of the analyses provide valuable suggestions for future research on HEV diversity.

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Further evidence reveals that okra mottle virus arose from a double recombination event

Archives of Virology ◽

10.1007/s00705-012-1458-9 ◽

2012 ◽

Vol 158 (1) ◽

pp. 181-186 ◽

Cited By ~ 4

Author(s):

Leonardo C. Albuquerque ◽

Silvia A. Aranha ◽

Fernanda R. Fernandes ◽

Alice K. Inoue-Nagata

Keyword(s):

Recombination Event ◽

Mottle Virus ◽

Double Recombination ◽

Double Recombination Event

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Evidence of Recombination between Divergent Hepatitis E Viruses

Journal of Virology ◽

10.1128/jvi.79.14.9306-9314.2005 ◽

2005 ◽

Vol 79 (14) ◽

pp. 9306-9314 ◽

Cited By ~ 32

Author(s):

Hélène van Cuyck ◽

Jun Fan ◽

David L. Robertson ◽

Pierre Roques

Keyword(s):

Phylogenetic Relationships ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Recombination Analysis ◽

First Time

ABSTRACT Phylogenetic and recombination analysis was performed on 32 complete hepatitis E virus (HEV) genomes from infected humans and pigs. For the first time, evidence for recombination between divergent HEV strains was obtained, with at least two strains being found to have discordant phylogenetic relationships consistent with the occurrence of intragenotype recombination. This finding confirms that humans can be dually infected with divergent HEV strains and has implications for the emergence and evolution of new HEV epidemics.

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