scholarly journals Norovirus recombination

2007 ◽  
Vol 88 (12) ◽  
pp. 3347-3359 ◽  
Author(s):  
Rowena A. Bull ◽  
Mark M. Tanaka ◽  
Peter A. White
Keyword(s):  
High Frequency ◽  
Driving Force ◽  
Recombination Event ◽  
Viral Evolution ◽  
Rna Recombination ◽  
Recombination Point ◽  
The Family ◽  
Double Recombination ◽  
Double Recombination Event ◽  
Recombination Breakpoints

RNA recombination is a significant driving force in viral evolution. Increased awareness of recombination within the genus Norovirus of the family Calicivirus has led to a rise in the identification of norovirus (NoV) recombinants and they are now reported at high frequency. Currently, there is no classification system for recombinant NoVs and a widely accepted recombinant genotyping system is still needed. Consequently, there is duplication in reporting of novel recombinants. This has led to difficulties in defining the number and types of recombinants in circulation. In this study, 120 NoV nucleotide sequences were compiled from the current GenBank database and published literature. NoV recombinants and their recombination breakpoints were identified using three methods: phylogenetic analysis, SimPlot analysis and the maximum χ 2 method. A total of 20 NoV recombinant types were identified in circulation worldwide. The recombination point is the ORF1/2 overlap in all isolates except one, which demonstrated a double recombination event within the polymerase region.

scholarly journals Feline Coronavirus Type II Strains 79-1683 and 79-1146 Originate from a Double Recombination between Feline Coronavirus Type I and Canine Coronavirus

1998 ◽  
Vol 72 (5) ◽  
pp. 4508-4514 ◽  
Author(s):  
Arnold A. P. M. Herrewegh ◽  
Ingrid Smeenk ◽  
Marian C. Horzinek ◽  
Peter J. M. Rottier ◽  
Raoul J. de Groot
Keyword(s):  
Recombination Event ◽  
Comparative Sequence Analysis ◽  
Type I ◽  
Type Ii ◽  
Rna Recombination ◽  
Recombinant Viruses ◽  
Canine Coronavirus ◽  
Double Recombination ◽  
Template Switch ◽  
Feline Coronavirus

ABSTRACT Recent evidence suggests that the type II feline coronavirus (FCoV) strains 79-1146 and 79-1683 have arisen from a homologous RNA recombination event between FCoV type I and canine coronavirus (CCV). In both cases, the template switch apparently took place between the S and M genes, giving rise to recombinant viruses which encode a CCV-like S protein and the M, N, 7a, and 7b proteins of FCoV type I (K. Motowaka, T. Hoh- datsu, H. Hashimoto, and H. Koyama, Microbiol. Immunol. 40:425–433, 1996; H. Vennema, A. Poland, K. Floyd Hawkins, and N. C. Pedersen, Feline Pract. 23:40–44, 1995). In the present study, we have looked for additional FCoV-CCV recombination sites. Four regions in the pol gene were selected for comparative sequence analysis of the type II FCoV strains 79-1683 and 79-1146, the type I FCoV strains TN406 and UCD1, the CCV strain K378, and the TGEV strain Purdue. Our data show that the type II FCoVs have arisen from double recombination events: additional crossover sites were mapped in the ORF1ab frameshifting region of strain 79-1683 and in the 5′ half of ORF1b of strain 79-1146.

scholarly journals Construction of an Unstable Ring-X Chromosome Bearing the Autosomal Dopa Decarboxylase Gene in Drosophila melanogaster and Analysis of Ddc Mosaics

Genetics ◽  
1987 ◽  
Vol 115 (2) ◽  
pp. 305-311
Author(s):  
Donald A Gailey ◽  
Deborah L Bordne ◽  
Ana Maria Vallés ◽  
Jeffrey C Hall ◽  
Kalpana White
Keyword(s):  
Nervous System ◽  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Recombination Event ◽  
P Element ◽  
Dopa Decarboxylase ◽  
Somatic Instability ◽  
Absolute Requirement ◽  
Double Recombination ◽  
Double Recombination Event

ABSTRACT An unstable Ring-X chromosome, Ddc  +- Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed. The construction involved a double recombination event between the unstable Ring-X, R(1)wvC and a Rod-X chromosome which contained a P-element mediated Ddc  + insert. The resulting Ddc  +-Ring-X chromosome behaves similarly to the parent chromosome with respect to somatic instability. The Ddc  +-Ring-X chromosome was used to generate Ddc mosaics. Analyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc  + expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.

scholarly journals Recombination analysis reveals a double recombination event in hepatitis E virus

2010 ◽  
Vol 7 (1) ◽  
pp. 129 ◽  
Author(s):  
Hua Wang ◽  
Wen Zhang ◽  
Bin Ni ◽  
Hongxing Shen ◽  
Yuyu Song ◽  
...  
Keyword(s):  
Recombination Event ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombination Analysis ◽  
Double Recombination ◽  
Double Recombination Event

Further evidence reveals that okra mottle virus arose from a double recombination event

2012 ◽  
Vol 158 (1) ◽  
pp. 181-186 ◽  
Author(s):  
Leonardo C. Albuquerque ◽  
Silvia A. Aranha ◽  
Fernanda R. Fernandes ◽  
Alice K. Inoue-Nagata
Keyword(s):  
Recombination Event ◽  
Mottle Virus ◽  
Double Recombination ◽  
Double Recombination Event

The Crispe Family and the African Trade in the Seventeenth Century

1968 ◽  
Vol 9 (1) ◽  
pp. 57-77 ◽  
Author(s):  
R. Porter
Keyword(s):  
Seventeenth Century ◽  
West Africa ◽  
Driving Force ◽  
Gold Coast ◽  
The Family ◽  
First Time

Nicholas Crispe (1598–1666) played a very important part in the developing of English trading contacts with West Africa in the seventeenth century. He obtained a commanding position within the African company in 1628 and did much to secure the company's reconstitution on a sounder basis in 1631. From 1631 until 1644 Crispe was the driving force behind the trade and, in particular, directed and largely financed the successful English entry into the gold trade of the Gold Coast, where permanent English factories with resident traders were established for the first time and a fort was started at Kormantin. After the Restoration he tried to regain his former position, but was unsuccessful, though his membership of the Company of Adventurers did give him some influence on the trade. Other members of the family were also involved in the African trade, sometimes in a significant way, over the same period.

scholarly journals Peranan Lingkungan Sosial terhadap Pembentukan Karakter Peduli Lingkungan Mahasiswa Pendidikan Geografi di Universitas Samudra Langsa

2018 ◽  
Vol 10 (2) ◽  
pp. 235
Author(s):  
Fitria Mustika ◽  
Tengku Muhammad Sahudra
Keyword(s):  
Family Environment ◽  
High Frequency ◽  
Low Frequency ◽  
Measurement Scale ◽  
Education Student ◽  
Achievement Level ◽  
Very High Frequency ◽  
Environmental Care ◽  
The Family

This study aims to determine the role of the family, community, and tertiary environment towards the formation of caring character in the geography education student environment. The output of this study was a four-indexed indexed education journal and a UNIMED google scolar. Samples from the study population were 20 respondents with 30 items about questionnaires. The time of the study starts from May 2018 to October 2018. The measurement scale in this study is the scale of the briquette. Data collection by means of library studies, interviews, questionnaires, and documentation. This research was conducted by distributing questionnaire questionnaires and interviews. Based on the results of questionnaire data processing, the family environment has a very high frequency in the formation of character of the student's environmental care with an achievement level of a total score of 91%. Furthermore, the role of the campus environment also has a high frequency in the formation of the character of the student's environmental care with a total score of 89%. Whereas the family environment has a low frequency in the formation of character of student environmental care with a total score of 57%. It can be concluded that the family environment has the most role in forming the character of the student's environmental care.

scholarly journals Strategi Pemberdayaan Wanita Nelayan Dalam Peningkatan Ekonomi Keluarga Nelayan di Gampong Langkak Kecamatan Kuala Pesisir Kabupaten Nagan Raya

2018 ◽  
Vol 3 (4) ◽  
pp. 262-269
Author(s):  
Cut Irma Aflandari ◽  
Sofyan Samsudin ◽  
Edy Marsudi
Keyword(s):  
Driving Force ◽  
Swot Analysis ◽  
Qualitative Approach ◽  
Women Empowerment ◽  
Fish Processing ◽  
Economic Activities ◽  
Family Activities ◽  
Family Economy ◽  
The Family

Abstrak. peran pemberdayaan wanita nelayan sangat di perlukan untuk membantu ekonomi keluarga. Wanita-wanita di daerah pesisir bisa menjadi motor penggerak kegiatan ekonomi produktif fungsinya dari ibu rumah tangga biasa menjadi pencari nafkah seperti melakukan kegiatan pengolahan ikan dan lain sebagainya. Penelitian ini bertujuan untuk mengetahui bentuk kegiatan keluarga nelayan di Gampong Langkak Kecamatan Kuala Pesisir Kabupaten Nagan Raya dalam  meningkatkan ekonomi keluarga , dan rencana strategi perempuan  nelayan dalam meningkatkan  ekonomi keluarga di Gampong Langkak Kecamatan Kuala Pesisir Kabupaten Nagan Raya. Metode yang digunakan dalam penelitian ini adalah pendekatan kualitatif dan analisis SWOT. Hasil dalam penelitian ini didapat strategi wo sebagai rencana strategi pemberdayaan wanita nelayan. Srategi wo yaitu memanfaatkan peluang yang ada dengan cara meminimalkan  kelemahan yang ada. Adapun bentuk kegiatan keluarga adalah bertani, jemur udang  sabu dan buruh pabrik.Kata kunci: pemberdayaan, keluarga , nelayan.Strategy Of Fishermen Women Empowerment In Increasing Famili Economy In Langkak  Village Kuala Pesisir  Districts Nagan Raya DistrictAbstract. the role of empowering women fishermen is very much needed to help the family economy. Women in coastal areas can be the driving force of productive economic activities whose function is from ordinary housewives to being breadwinners such as fish processing activities and so on. This study aims to determine the form of fishermen's family activities in Gampong Langkak, Kuala Pesisir Subdistrict, Nagan Raya District in improving the family's economy, and the strategy of women fishermen in improving the family's economy in Ganggong Langkak, Kuala Pesisir District, Nagan Raya District. The method used in this study is a qualitative approach and SWOT analysis. The results in this study obtained the WO strategy as a plan to empower women fishermen strategies. The strategy is to take advantage of existing opportunities by minimizing existing weaknesses. The form of family activities is farming, drying shabu shrimp and factory workers.Keywords : empowerment, family, fishermen.  

scholarly journals High frequency RNA recombination in porcine reproductive and respiratory syndrome virus occurs preferentially between parental sequences with high similarity

2001 ◽  
Vol 82 (11) ◽  
pp. 2615-2620 ◽  
Author(s):  
Joke J. F. A. van Vugt ◽  
Torben Storgaard ◽  
Martin B. Oleksiewicz ◽  
Anette Bøtner
Keyword(s):  
North American ◽  
High Frequency ◽  
Respiratory Syndrome Virus ◽  
European American ◽  
Rna Recombination ◽  
High Similarity ◽  
Rt Pcr ◽  
European Isolate ◽  
North American Type ◽  
Theoretical Risk

Two types of porcine reproductive and respiratory syndrome virus (PRRSV) exist, a North American type and a European type. The co-existence of both types in some countries, such as Denmark, Slovakia and Canada, creates a risk of inter-type recombination. To evaluate this risk, cell cultures were co-infected with either a North American and a European type of PRRSV or two diverse types of European isolate. Subsequently, an approximately 600 bp region of the PRRSV genome was tested for recombination by quantitative real-time RT–PCR. Between 0·1 and 2·5% RNA recombination was found between the European isolates, but no recombination was detected between the European and North American types. Calculation of the maximum theoretical risk of European–American recombination, based on the sensitivity of the RT–PCR system, revealed that RNA recombination between the European and North American types of PRRSV is at least 10000 times less likely to occur than RNA recombination between diverse European isolates.

scholarly journals Sundown syndrome in patients with Alzheimer’s disease dementia

2019 ◽  
Vol 13 (4) ◽  
pp. 469-474 ◽  
Author(s):  
Cristiani Sartorio Menegardo ◽  
Fernanda Alencar Friggi ◽  
Julia Baldon Scardini ◽  
Tais Souza Rossi ◽  
Thais dos Santos Vieira ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Frequency ◽  
Neuropsychiatric Symptoms ◽  
Time Of Day ◽  
Neuropsychiatric Inventory ◽  
Cross Sectional ◽  
Symptom Intensity ◽  
The Family ◽  
Alzheimer’S Disease Dementia

ABSTRACT The sundown syndrome is a complex neurobehavioral disorder in patients with dementia associated with high financial cost and significant caregiver burden. It is a multifactorial phenomenon with unclear pathophysiology, characterized by the presence of neuropsychiatric symptoms in the evening period. Objective: To analyze the main neuropsychiatric symptoms, their correlation with one another, with comorbidities, and with time of day of greatest symptom intensity in patients with Alzheimer’s disease dementia. Methods: This is a cross-sectional, observational and explanatory study in which caregivers/relatives of elderly patients with dementia were interviewed using a structured tool called the Neuropsychiatric Inventory (NPI). Results: The sample studied was composed of 38 patients, 60.5% female and 39.5% male, with mean age of 81±6 (67-94) years. A high frequency of neuropsychiatric symptoms in the evening period was observed, predominantly irritability (55.3%), nocturnal behavior (47.4%), and aggressiveness (42.1%). Only 36.8% of the family caregivers used non-pharmacological strategies. Conclusion: The frequency of neuropsychiatric symptoms was exacerbated in the evening among patients with Alzheimer’s disease, especially for those behavioral symptoms that had a positive correlation with one another.

scholarly journals Recombination of Feline calicivirus within an endemically infected cat colony

2006 ◽  
Vol 87 (4) ◽  
pp. 921-926 ◽  
Author(s):  
K. P. Coyne ◽  
F. C. Reed ◽  
C. J. Porter ◽  
S. Dawson ◽  
R. M. Gaskell ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Recombination Event ◽  
Common Mechanism ◽  
Feline Calicivirus ◽  
First Report ◽  
The Family ◽  
Distinct Strain ◽  
First Time

To understand the evolution of the family Caliciviridae, the persistence of Feline calicivirus (FCV) was studied within an endemically infected cat colony. Polymerase and capsid sequences were analysed for 34 FCV isolates obtained over a 4 year period. Initially, the colony was infected with one strain of virus, but a second distinct strain was later identified. Subsequently, the emergence of a recombinant virus was observed, containing elements of both of the strains circulating within the colony. The recombination event mapped close to the ORF1/ORF2 junction. This is consistent with recombination in other caliciviruses, suggesting a common mechanism within this family. This is the first report of recombination within the genus Vesivirus in the family Caliciviridae and the first time that a recombination event has been observed where the parental strains have also been identified.

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