Synergistic action of curcumin and cisplatin on spinel ferrite/hierarchical MCM-41 nanocomposite against MCF-7, HeLa and HCT 116 cancer cell line

Abstract Background Platinum-based drugs are widely used in cancer therapy, but are known for toxic side effects and resistance. Combinational drug delivery represents an effective chemotherapeutic strategy, but often leads to an increased toxicity. Aim of this study is to test the co-delivery of cisplatin with natural antioxidants on hierarchial porous large surface area hexagonal nanocarriers for synergistic action. Results A series of structured mesoporous materials were impregnated with magnetic spinel ferrite (30% CuFe2O4) and then coated with curcumin (25% wt/wt). Mesosilicalite and MCM-41 with high curcumin release abilities were functionalized with cisplatin (5% wt/wt) for synergistic effect of combinational drugs. The cytotoxic efficiency of our nanocomposites was tested on cell viability of MCF7 (human breast cancer), human cervical cancer (HeLa), colorectal cancer (HCT116), and HFF (human foreskin fibroblasts) cell lines using the MTT cell viability assay. At a concentration of 0.1 mg/ml, CuFe2O4/mesosilicalite/curcumin/cisplatin resulted in 89.53% reduction in viability in MCF7, 94.03% in HeLa, 64% in HCT116 and 87% in HFF; whereas, CuFe2O4/MCM-41/curcumin/cisplatin resulted in 76% reduction in viability in MCF7, 64.46% in HeLa, 64% in HCT116 and 24% in HFF. The EC50 for CuFe2O4/mesosilicalite/curcumin/cisplatin was 81.23 µg/ml in MCF7, 47.55 µg/ml in HeLa, 48.96 µg/ml in HCT116 and 76.83 µg/ml in HFF. The EC50 for CuFe2O4/MCM-41/curcumin/cisplatin was 72.51 µg/ml in MCF7, 58.6 µg/ml in HeLa, 62.58 µg/ml in HCT116 and 154.2 µg/ml in HFF. Furthermore, cells treated with both nanocomposites had a high number of cleaved Caspase 3-positive cells suggesting that the reduction in cell viability was triggered by activating the apoptotic signaling pathway. Conclusion Our results show that CuFe2O4/MCM-41/curcumin/cisplatin is a better candidate for combinational drug therapy due to its lowest EC50 value and the wider difference in EC50 (a fold change) between cancerous and non-cancerous cell line.

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Antimelanoma and Antityrosinase fromAlpinia galangalConstituents

The Scientific World JOURNAL ◽

10.1155/2013/186505 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 15

Author(s):

Chih-Yu Lo ◽

Po-Len Liu ◽

Li-Ching Lin ◽

Yen-Ting Chen ◽

You-Cheng Hseu ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Food Industry ◽

Human Melanoma ◽

Therapeutic Application ◽

Cell Viability Assay ◽

Viability Assay ◽

Anticancer Effects ◽

Spectroscopic Analyses ◽

Melanin Contents

Two compounds, 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (BHPHTO) and bisdemethoxycurcumin (BDMC) they have been isolated from the rhizomes ofAlpinia galangal, and the structures of both pure constituents were determined using spectroscopic analyses. The study examined the bioeffectivenesses of the two compounds on the human melanoma A2058 and showed that significantly inhibited the proliferation of melanoma cells in the cell viability assay. This research was also taken on the tests to B16-F10 cell line and showed minor inhibitory consequences of cellular tyrosinase activities and melanin contents. Our results revealed the anticancer effects ofA. galangalcompounds, and therefore, the target compounds could be potentially applied in the therapeutic application and the food industry.

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Interference in MTT cell viability assay in activated macrophage cell line

Analytical Biochemistry ◽

10.1016/s0003-2697(02)00631-0 ◽

2003 ◽

Vol 313 (2) ◽

pp. 338-341 ◽

Cited By ~ 52

Author(s):

Marina Pozzolini ◽

Sonia Scarfı̀ ◽

Umberto Benatti ◽

Marco Giovine

Keyword(s):

Cell Viability ◽

Cell Line ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Cell Viability Assay ◽

Viability Assay ◽

Activated Macrophage

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Anti-inflammatory activity of Myristica fragrans extract

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i4.1607 ◽

2019 ◽

Vol 10 (4) ◽

pp. 3118-3120

Author(s):

Farhat Yaasmeen Sadique Basha ◽

Rajeshkumar S ◽

Lakshmi T

Keyword(s):

Cell Viability ◽

Cell Line ◽

Mtt Assay ◽

Inflammatory Cells ◽

The Body ◽

Inflammatory Activity ◽

Cell Viability Assay ◽

Viability Assay ◽

Myristica Fragrans ◽

Anti Inflammatory

In simple terms, inflammation can be defined as a reaction from the body to an injury in living tissue. Anti-inflammatory drugs help in controlling and reducing this inflammation. Natural spices showing anti-inflammatory properties with no side effects, hence they can be used as an efficient anti-inflammatory drug in the near future. To determine the anti-inflammatory activity of Myristica fragrans (Nutmeg) using MTT Assay. The plant material was obtained as a gift sample from Life Care Phytolabs Private Limited. An extract was prepared from the sample. Cell viability assay – MTT Assay was performed, and Raw cell line 247 was used to study the anti-inflammatory potential of the extract. The results collected were put into a graph and table for discussion. A gradual decrease in the number of inflammatory cells as the concentration of the extract was increased was observed in the inflammatory cell line. The cell viability, which was 7.08% when the concentration of the extract was 1ng increased up to 30.6% when the concentration of the extract was increased up to 100ug. The MTT assay test on a raw cell line 247 showed that the Myristica fragrans extract exhibits some level of the anti-inflammatory property. Further research on isolating the specific component of the extract responsible for its anti-inflammatory property can be done in the future.

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Do We have a Satisfactory Cell Viability Assay? Review of the Currently Commercially-Available Assays

Current Drug Discovery Technologies ◽

10.2174/1570163815666180925095433 ◽

2020 ◽

Vol 17 (1) ◽

pp. 2-22 ◽

Cited By ~ 3

Author(s):

Abdel-Baset Halim

Keyword(s):

Cell Viability ◽

Data Interpretation ◽

Positive Control ◽

Live Cells ◽

Proof Of Concept ◽

Cell Viability Assay ◽

Viability Assay ◽

Current Cell ◽

Dying Cells ◽

Differential Permeability

:Cell-based assays are an important part of the drug discovery process and clinical research. One of the main hurdles is to design sufficiently robust assays with adequate signal to noise parameters while maintaining the inherent physiology of the cells and not interfering with the pharmacology of target being investigated.:A plethora of assays that assess cell viability (or cell heath in general) are commercially available and can be classified under different categories according to their concepts and principle of reactions. The assays are valuable tools, however, suffer from a large number of limitations. Some of these limitations can be procedural or operational, but others can be critical as those related to a poor concept or the lack of proof of concept of an assay, e.g. those relying on differential permeability of dyes in-and-out of viable versus compromised cell membranes. While the assays can differentiate between dead and live cells, most, if not all, of them can just assess the relative performance of cells rather than providing a clear distinction between healthy and dying cells. The possible impact of relatively high molecular weight dyes, used in most of the assay, on cell viability has not been addressed. More innovative assays are needed, and until better alternatives are developed, setup of current cell-based studies and data interpretation should be made with the limitations in mind. Negative and positive control should be considered whenever feasible. Also, researchers should use more than one orthogonal method for better assessment of cell health.

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Exploring the Noncovalent Interactions of the Dinuclear Cu(II) Schiff Base Complex with Bovine Serum Albumin and Cell Viability against the SiHa Cancer Cell Line

The Journal of Physical Chemistry B ◽

10.1021/acs.jpcb.1c05794 ◽

2021 ◽

Author(s):

Ribhu Maity ◽

Nayim Sepay ◽

Ushasi Pramanik ◽

Kalyanmoy Jana ◽

Saptarshi Mukherjee ◽

...

Keyword(s):

Schiff Base ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Cell Viability ◽

Cell Line ◽

Cancer Cell ◽

Bovine Serum ◽

Noncovalent Interactions ◽

Schiff Base Complex ◽

Cancer Cell Line

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Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v12i1.28714 ◽

2017 ◽

Vol 12 (1) ◽

pp. 35 ◽

Cited By ~ 7

Author(s):

Dian Ratih Laksmitawati ◽

Anisa Widyastuti ◽

Nadia Karami ◽

Ervi Afifah ◽

Dwi Davidson Rihibiha ◽

...

Keyword(s):

Cell Line ◽

Tumor Necrosis ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Cell Viability Assay ◽

Viability Assay ◽

Tnf Α ◽

Anti Inflammatory ◽

Α Level ◽

Necrosis Factor

In this study, the anti-inflammatory potential of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1β (IL-1β), tumor necrosis factor (TNF)-α, nitric oxide (NO) and IL-6. Both A. cordifolia and P. crocatum at concentration of 50 µg/mL in cell line resulted significant decrease in TNF-α level (250.3 and 242.5 pg/mL respectively). A. cordifolia showed significant decrease in IL-1β level at 50 µg/mL and IL-6 level at 10 µg/mL, whilst P. crocatum showed significant decrease IL-1β level in three concentrations with lowest level at 50 µg/mL. A. cordifolia showed lowest decrease in NO level at 50 µg/mL but not comparable with normal cells, whilst P. crocatum showed significant decrease in NO level at 50 µg/mL. This research revealed that A. cordifolia and P. crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-α, IL-1β, IL-6, and NO.

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Promising biocompatible hydrogels of crosslinked polyelectrolytes for biomedical applications

Current Directions in Biomedical Engineering ◽

10.1515/cdbme-2017-0147 ◽

2017 ◽

Vol 3 (2) ◽

pp. 695-698

Author(s):

Andreas Brietzke ◽

Christian von der Ehe ◽

Sabine Illner ◽

Claudia Matschegewski ◽

Niels Grabow ◽

...

Keyword(s):

Aqueous Solution ◽

Ionic Liquids ◽

Cell Viability ◽

Biomedical Applications ◽

High Potential ◽

Cell Viability Assay ◽

Mouse Fibroblasts ◽

Viability Assay ◽

Intelligent Implant ◽

L929 Mouse

AbstractFor the development of intelligent implant systems hydrogels (HG) from crosslinked ionic liquids feature a high potential to be utilised as a drug depot. Biocompatibility of the HGs is one key prerequisite for biomedical applications. HGs were polymerised from a variety of different ionic monomers based on methacrylate, methacrylamide, styrene or vinyl imidazolium derivatives in aqueous solution. N,N'-methylenebisacrylamide was used as crosslinker. CellQuanti-Blue™ Cell Viability Assay Kit was implemented to proof viability of L929 mouse fibroblasts. The predominant part of the HG eluates generated only a marginal reduction of less than 15% cell viability at 100% eluate concentration. This underlines the excellent suitability of these HGs for biomedical applications and revealed some promising candidates for the development of drug depots for implants.

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Sensitive cell viability assay for use in drug screens and for studying the mechanism of action of drugs inDictyostelium discoideum

BioTechniques ◽

10.2144/000112260 ◽

2006 ◽

Vol 41 (5) ◽

pp. 591-595 ◽

Cited By ~ 6

Author(s):

Junxia Min ◽

Priya Sridevi ◽

Stephen Alexander ◽

Hannah Alexander

Keyword(s):

Cell Viability ◽

Mechanism Of Action ◽

Sensitive Cell ◽

Cell Viability Assay ◽

Viability Assay

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Synthesis of Novel FTY720 Analogs with Anticancer Activity through PP2A Activation

Molecules ◽

10.3390/molecules23112750 ◽

2018 ◽

Vol 23 (11) ◽

pp. 2750 ◽

Cited By ~ 4

Author(s):

Jitendra Shrestha ◽

Sung Ki ◽

Sang Shin ◽

Seon Kim ◽

Joo-Youn Lee ◽

...

Keyword(s):

Cell Viability ◽

Anticancer Activity ◽

Cancer Cells ◽

Sphingosine Kinase ◽

Basic Structure ◽

Docking Study ◽

Cell Viability Assay ◽

Viability Assay ◽

Anticancer Effects ◽

Pp2a Activation

FTY720 inhibits various cancers through PP2A activation. The structure of FTY720 is also used as a basic structure for the design of sphingosine kinase (SK) inhibitors. We have synthesized derivatives using an amide chain in FTY720 with a phenyl backbone, and then compounds were screened by an MTT cell viability assay. The PP2A activity of compound 7 was examined. The phosphorylation levels of AKT and ERK, downstream targets of PP2A, in the presence of compound 7, were determined. Compound 7 may exhibit anticancer effects through PP2A activation rather than the mechanism by inhibition of SK1 in cancer cells. In the docking study of compound 7 and PP2A, the amide chain of compound 7 showed an interaction with Asn61 that was different from FTY720, which is expected to affect the activity of the compound.

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