scholarly journals Anti-inflammatory activity of Myristica fragrans extract

2019 ◽  
Vol 10 (4) ◽  
pp. 3118-3120
Author(s):  
Farhat Yaasmeen Sadique Basha ◽  
Rajeshkumar S ◽  
Lakshmi T
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Mtt Assay ◽  
Inflammatory Cells ◽  
The Body ◽  
Inflammatory Activity ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Myristica Fragrans ◽  
Anti Inflammatory

In simple terms, inflammation can be defined as a reaction from the body to an injury in living tissue. Anti-inflammatory drugs help in controlling and reducing this inflammation. Natural spices showing anti-inflammatory properties with no side effects, hence they can be used as an efficient anti-inflammatory drug in the near future. To determine the anti-inflammatory activity of Myristica fragrans (Nutmeg) using MTT Assay. The plant material was obtained as a gift sample from Life Care Phytolabs Private Limited. An extract was prepared from the sample. Cell viability assay – MTT Assay was performed, and Raw cell line 247 was used to study the anti-inflammatory potential of the extract. The results collected were put into a graph and table for discussion. A gradual decrease in the number of inflammatory cells as the concentration of the extract was increased was observed in the inflammatory cell line. The cell viability, which was 7.08% when the concentration of the extract was 1ng increased up to 30.6% when the concentration of the extract was increased up to 100ug. The MTT assay test on a raw cell line 247 showed that the Myristica fragrans extract exhibits some level of the anti-inflammatory property. Further research on isolating the specific component of the extract responsible for its anti-inflammatory property can be done in the future.

scholarly journals Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line

2017 ◽  
Vol 12 (1) ◽  
pp. 35 ◽  
Author(s):  
Dian Ratih Laksmitawati ◽  
Anisa Widyastuti ◽  
Nadia Karami ◽  
Ervi Afifah ◽  
Dwi Davidson Rihibiha ◽  
...  
Keyword(s):  
Cell Line ◽  
Tumor Necrosis ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Α Level ◽  
Necrosis Factor

<p class="Abstract">In this study, the anti-inflammatory potential of <em>Anredera </em>cordifolia and <em>Piper </em>crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1β (IL-1β), tumor necrosis factor (TNF)-α, nitric oxide (NO) and IL-6. Both <em>A. </em>cordifolia and<em> P. </em>crocatum at concentration of 50 µg/mL in cell line resulted significant decrease in TNF-α level (250.3 and 242.5 pg/mL respectively). <em>A. </em>cordifolia showed significant decrease in IL-1β level at 50 µg/mL and IL-6 level at 10 µg/mL, whilst <em>P. </em>crocatum  showed significant decrease IL-1β level in three concentrations with lowest level at 50 µg/mL.<em> A. </em>cordifolia showed lowest decrease in NO level at 50 µg/mL but not comparable with normal cells, whilst <em>P. </em>crocatum showed significant decrease in NO level at 50 µg/mL. This research revealed that <em>A. </em>cordifolia and<em> P. </em>crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-α, IL-1β, IL-6, and NO.</p>

scholarly journals Antimelanoma and Antityrosinase fromAlpinia galangalConstituents

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Chih-Yu Lo ◽  
Po-Len Liu ◽  
Li-Ching Lin ◽  
Yen-Ting Chen ◽  
You-Cheng Hseu ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Food Industry ◽  
Human Melanoma ◽  
Therapeutic Application ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Anticancer Effects ◽  
Spectroscopic Analyses ◽  
Melanin Contents

Two compounds, 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (BHPHTO) and bisdemethoxycurcumin (BDMC) they have been isolated from the rhizomes ofAlpinia galangal, and the structures of both pure constituents were determined using spectroscopic analyses. The study examined the bioeffectivenesses of the two compounds on the human melanoma A2058 and showed that significantly inhibited the proliferation of melanoma cells in the cell viability assay. This research was also taken on the tests to B16-F10 cell line and showed minor inhibitory consequences of cellular tyrosinase activities and melanin contents. Our results revealed the anticancer effects ofA. galangalcompounds, and therefore, the target compounds could be potentially applied in the therapeutic application and the food industry.

Interference in MTT cell viability assay in activated macrophage cell line

2003 ◽  
Vol 313 (2) ◽  
pp. 338-341 ◽  
Author(s):  
Marina Pozzolini ◽  
Sonia Scarfı̀ ◽  
Umberto Benatti ◽  
Marco Giovine
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Activated Macrophage

scholarly journals Effects of Hyeonggaeyeongyo-Tang in Ovalbumin-Induced Allergic Rhinitis Model

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Se Hyang Hong ◽  
Soon Re Kim ◽  
Han-Seok Choi ◽  
Jin Mo Ku ◽  
Hye Sook Seo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Cell Viability ◽  
Allergic Inflammation ◽  
Inflammatory Cells ◽  
Inhibitory Effect ◽  
Blood Analysis ◽  
Cell Viability Assay ◽  
Nasal Airways ◽  
Viability Assay

Allergic rhinitis (AR) is an allergic inflammation of the nasal airways. The prevalence of AR is increasing worldwide. We investigated whether Hyeonggaeyeongyo-tang (HYT) is effective to suppress the progression of AR induced by ovalbumin (OVA). Male BALB/c mice were used for this study. Allergic rhinitis was induced by OVA. Treatment with HYT was assessed to study the effect of HYT on allergic rhinitis in mice. Histological analysis, immunohistochemistry, multiplex cytokine assay, blood analysis, and cell viability assay were performed to verify inhibitory effect of HYT on allergic rhinitis. HYT did not show any toxicity maintaining body weight. Food intake was steady without variation in mice. HYT reduced infiltration of inflammatory cells and mast cells into nasal cavity. HYT reduced the levels of cytokines and leukocytes in the blood. HYT decreased the splenocyte cell viability. Antihistamines and steroids are the most common medications used to treat allergic rhinitis. However, long-term use of drug generates resistance or side effects requiring the development of new drug. Our present study clearly demonstrates that HYT suppresses the progression of allergic rhinitis induced by OVA. This suggests that HYT might be a useful drug for the treatment of allergic rhinitis.

Exploring the phytochemical and nutraceutical potentials of dasapatrachurnam

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Rasajna Nadella ◽  
Daniel Hernandez-Baltazar ◽  
John Sushma Nannepaga ◽  
Balamani Venkata Annapurna Gorthi ◽  
Daniel Martinez-Fong
Keyword(s):  
Leafy Vegetables ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Bacteriostatic Activity ◽  
Bacterial Strains ◽  
Cell Viability Assay ◽  
Green Leafy Vegetables ◽  
Viability Assay ◽  
Anti Inflammatory

AbstractBackgroundDasapatrachurnam (DPC), a multicurative powder prepared from the leaves of 10 green leafy vegetables, was developed recently with known ethnobotanical and ethnopharmacological significance. However, its functional role in curing a disease is not yet scientifically proven. The present study aims at performing the phytochemical screening of DPC and exploring its possible activity as bacteriostatic, antineoplastic and anti-inflammatory.MethodsWe performed qualitative and Fourier transform infrared spectroscopy (FTIR) to find out the presence of active compounds and tested the bacteriostatic activity in four bacterial strains namely Bacillus subtilis, Escherichia coli, Streptococcus pyogenes and Staphylococcus aureus by agar well diffusion method. We further explored the antineoplastic activity in vitro in C6 and HEK293 cell lines by cell viability assay and the anti-inflammatory activity in the ovalbumin-induced inflammation in male Wistar rats.ResultsDPC showed 60% solubility in PBS and showed the presence of flavonoids and glycosides. FTIR results indicated the presence of alkyl, ketone and aldehyde groups. The bacteriostatic activity of DPC was higher (60%) in E.coli and lower (8%) in S.aureus, when compared to streptomycin. The anti-cancerous activity of DPC in C6 and HEK293 cancer cells was similar to their respective positive controls, curcumin and camptothecin. The anti-inflammatory activity of DPC was more evident with local administration in all the parameters studied in brain hippocampus, kidney, liver and spleen in ovalbumin-induced rats.ConclusionOur results, for the first time, suggest the potentiality of the DPC in treating bacterial diseases, cancer and also inflammation. Our results also suggest the possible therapeutic role of DPC in treating chronic kidney disease.

scholarly journals Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup (Annona muricata L) Extracts in LPS Stimulated-Macrophage Cell (RAW264.7)

2016 ◽  
Vol 16 (2) ◽  
pp. 73 ◽  
Author(s):  
Dian Ratih Laksmitawati ◽  
Ajeng Prima Prasanti ◽  
Nadia Larasinta ◽  
Gloria Agitha Syauta ◽  
Rivanny Hilda ◽  
...  
Keyword(s):  
Cell Line ◽  
Colorimetric Assay ◽  
Macrophage Cell ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Biological Responses ◽  
High Viability ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Α Level

Inflammation is one of the important biological responses to injury. Anti-inflammatory is therefore proposed to treat both acute and chronic inflammation. Chemical compounds of various plants are widely used in treatment of inflammation. <strong>Objective:</strong> This study aims to evaluate anti-inflammatory potential of <em>G. vulgaris</em> extract (GVE) and <em>A. muricata</em> extract (AME) on LPS-stimulated murine macrophage cell line (RAW264.7). Cell viability assay to evaluate nontoxic concentration in cell line was performed with MTS assay. Parameters to determine anti-inflammatory activity between treatment group and non treated cells, were IL-1β, TNF-α, and IL-6 which was measured with Elisa, and NO level which was measured with nitrate/nitrite colorimetric assay. Both GVE and AME of 50 and 10 μg/mL showed high viability (&gt;90%) and it was not significantly different compared to control, makes it suitable for treatment. GVE and AME of 50 μg/mL resulted low TNF-α level in RAW264.7(313.16pg/mL and 264.69 pg/mL respectively), as well as IL-1β level (903.53 pg/mL and 905.00 pg/mL respectively) and IL-6 (175.88 pg/mL and 219.13 pg/mL respectively). Whereas, GVE and AME of 75 μg/mL showed lower NO level (9.76 μM and 9.79 μM respectively) compared to untreated cells. This research revealed that GVE and AME possess the anti-inflammatory potential indicated by inhibition of inflammatory mediators including TNF-α, IL-1β, IL-6 and NO.

scholarly journals Evaluation of Anti-Inflammatory Activity ofCitrus latifoliaTanaka Essential Oil and Limonene in Experimental Mouse Models

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Raquel Kummer ◽  
Fernanda Carolina Fachini-Queiroz ◽  
Camila Fernanda Estevão-Silva ◽  
Renata Grespan ◽  
Expedito Leite Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Folk Medicine ◽  
Neutrophil Migration ◽  
Peritoneal Exudate ◽  
Inflammatory Activity ◽  
Cell Viability Assay ◽  
Proinflammatory Mediators ◽  
Viability Assay ◽  
Anti Inflammatory

The genusCitrus(Rutaceae) includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity ofCitrus latifoliaTanaka essential oil (CLEO) and its main constituent LIM. In the cell viability assay, CLEO and LIM (3, 10, 30, and 90 μg/mL) had low cytotoxicity. In zymosan-induced peritonitis, LIM (500 mg/kg) decreased the infiltration of peritoneal exudate leukocytes and decreased the number of polymorphonuclear leukocytes.In vitrochemotaxis revealed that CLEO and LIM (1, 3, and 10 µg/mL) promoted a significant reduction of neutrophil migration toward fMLP and LTB4. LIM (500 mg/kg) also reduced TNF-αlevels but did not alter IL-10 levels in the peritoneal exudate. In conclusion, this study showed that LIM isolated from CLEO had potential anti-inflammatory effects, likely by inhibiting proinflammatory mediators present in inflammatory exudate and leukocyte chemotaxis.

scholarly journals In Vitro Evaluation of Substantivity, Staining Potential, and Biofilm Reduction of Guava Leaf Extract Mouth Rinse in Combination with its Anti-Inflammatory Effect on Human Gingival Epithelial Keratinocytes

Materials ◽  
2019 ◽  
Vol 12 (23) ◽  
pp. 3903
Author(s):  
J. Varghese ◽  
L. Ramenzoni ◽  
P. Shenoy ◽  
U. Nayak ◽  
N. Nayak ◽  
...  
Keyword(s):  
Cell Viability ◽  
Colorimetric Assay ◽  
Oral Care ◽  
Mouth Rinse ◽  
Cell Viability Assay ◽  
Oral Rinse ◽  
Viability Assay ◽  
Anti Inflammatory ◽  
Guava Leaf

This study aimed to assess the biofilm reduction, staining potential, and cytotoxicity of guava extract mouth rinse compared to chlorhexidine (CHX). Substantivity, staining, and antibiofilm potential were investigated by spectrophotometry, colony-forming units, and luminosity color meter, respectively. The cell viability assay was conducted using a colorimetric assay to determine nontoxic levels of guava (0.15%) and CHX in human gingival epithelial keratinocytes (HGEK-16). Cells were treated with lipopolysaccharides (LPS, 1μg/mL) and guava to assess inflammatory gene expression levels of interleukin-β1, tumor necrosis factor-α, and Prostaglandin E2. A scratch wound healing assay investigated the effects of guava on cell migration. The teeth coated in guava mouth rinse displayed 19.4% higher substantivity compared to CHX (0.2%), and the anti-biofilm reduction was observed with both guava and CHX mouth rinses (P < 0.05). The overall discoloration changes were higher with CHX and distilled water compared to guava. Also, guava significantly enhanced HGEK-16 cell viability (P < 0.05), and IL-β1, TNFα and PGE2 expression presented a 0.6-fold decrease when exposed to guava and LPS (P < 0.05). The present study showed that guava mouth rinse fulfilled the requirement for an effective and useful oral care product with desirable substantivity and anti-biofilm action. In addition, guava reduced the inflammation response in HGEK-16 and may be a potential oral rinse for oral anti-inflammatory therapies.

scholarly journals Synergistic action of curcumin and cisplatin on spinel ferrite/hierarchical MCM-41 nanocomposite against MCF-7, HeLa and HCT 116 cancer cell line

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
B. Rabindran Jermy ◽  
D. Almohazey ◽  
W. A. Alamoudi ◽  
R. M. Palanivel ◽  
Nora AlSudairi ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Spinel Ferrite ◽  
Cancer Cell Line ◽  
Natural Antioxidants ◽  
Synergistic Action ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Human Foreskin Fibroblasts ◽  
Mcm 41

Abstract Background Platinum-based drugs are widely used in cancer therapy, but are known for toxic side effects and resistance. Combinational drug delivery represents an effective chemotherapeutic strategy, but often leads to an increased toxicity. Aim of this study is to test the co-delivery of cisplatin with natural antioxidants on hierarchial porous large surface area hexagonal nanocarriers for synergistic action. Results A series of structured mesoporous materials were impregnated with magnetic spinel ferrite (30% CuFe2O4) and then coated with curcumin (25% wt/wt). Mesosilicalite and MCM-41 with high curcumin release abilities were functionalized with cisplatin (5% wt/wt) for synergistic effect of combinational drugs. The cytotoxic efficiency of our nanocomposites was tested on cell viability of MCF7 (human breast cancer), human cervical cancer (HeLa), colorectal cancer (HCT116), and HFF (human foreskin fibroblasts) cell lines using the MTT cell viability assay. At a concentration of 0.1 mg/ml, CuFe2O4/mesosilicalite/curcumin/cisplatin resulted in 89.53% reduction in viability in MCF7, 94.03% in HeLa, 64% in HCT116 and 87% in HFF; whereas, CuFe2O4/MCM-41/curcumin/cisplatin resulted in 76% reduction in viability in MCF7, 64.46% in HeLa, 64% in HCT116 and 24% in HFF. The EC50 for CuFe2O4/mesosilicalite/curcumin/cisplatin was 81.23 µg/ml in MCF7, 47.55 µg/ml in HeLa, 48.96 µg/ml in HCT116 and 76.83 µg/ml in HFF. The EC50 for CuFe2O4/MCM-41/curcumin/cisplatin was 72.51 µg/ml in MCF7, 58.6 µg/ml in HeLa, 62.58 µg/ml in HCT116 and 154.2 µg/ml in HFF. Furthermore, cells treated with both nanocomposites had a high number of cleaved Caspase 3-positive cells suggesting that the reduction in cell viability was triggered by activating the apoptotic signaling pathway. Conclusion Our results show that CuFe2O4/MCM-41/curcumin/cisplatin is a better candidate for combinational drug therapy due to its lowest EC50 value and the wider difference in EC50 (a fold change) between cancerous and non-cancerous cell line.

scholarly journals Anti-inflammatory activity of raw and processed stingless bee honey

Food Research ◽  
2021 ◽  
Vol 5 (S1) ◽  
pp. 201-208
Author(s):  
K.Y. Chong ◽  
N.L. Chin ◽  
Y.A. Yusof ◽  
S. Fakurazi
Keyword(s):  
Stingless Bee ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Cell Viability Assay ◽  
Mixed Effect ◽  
Viability Assay ◽  
No Inhibition ◽  
Anti Inflammatory

The anti-inflammatory activity of raw and processed Kelulut stingless bee honey was investigated for its ability to inhibit nitric oxide (NO) production in lipopolysaccharidestimulated RAW 264.7 cells. Raw honey was optimally processed by thermal processing and thermosonication at 90°C. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) cell viability assay showed that Kelulut honey from 7.8 to 500 µg/mL was not cytotoxic to RAW 264.7 cells as it resulted in at least 80% viable cells after 24 hr. Both raw and processed honey from 10 to 300 µg/mL displayed an increase and decrease in NO concentrations, suggesting a mixed effect of NO inhibition and enhancement. Maximum NO inhibition of 17.5% was recorded from 20 µg/mL of thermally processed honey while the highest NO enhancement of 7.8% was from 10 µg/mL of thermosonicated honey. The NO effects were independent of honey concentration and processing techniques, suggesting its potential robustness in medicinal properties as part of the diet.

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