Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA

AbstractNontuberculous mycobacteria, NTM, are of growing concern and among these members of the Mycobacterium mucogenicum (Mmuc) and Mycobacterium neoaurum (Mneo) clades can cause infections in humans and they are resistant to first-line anti-tuberculosis drugs. They can be isolated from different ecological niches such as soil, tap water and ground water. Mycobacteria, such as Mmuc and Mneo, are classified as rapid growing mycobacteria, RGM, while the most familiar, Mycobacterium tuberculosis, belongs to the slow growing mycobacteria, SGM. Modern “omics” approaches have provided new insights into our understanding of the biology and evolution of this group of bacteria. Here we present comparative genomics data for seventeen NTM of which sixteen belong to the Mmuc- and Mneo-clades. Focusing on virulence genes, including genes encoding sigma/anti-sigma factors, serine threonine protein kinases (STPK), type VII (ESX genes) secretion systems and mammalian cell entry (Mce) factors we provide insight into their presence as well as phylogenetic relationship in the case of the sigma/anti-sigma factors and STPKs. Our data further suggest that these NTM lack ESX-5 and Mce2 genes, which are known to affect virulence. In this context, Mmuc- and Mneo-clade members lack several of the genes in the glycopeptidolipid (GLP) locus, which have roles in colony morphotype appearance and virulence. For the M. mucogenicum type strain, MmucT, we provide RNASeq data focusing on mRNA levels for sigma factors, STPK, ESX proteins and Mce proteins. These data are discussed and compared to in particular the SGM and fish pathogen Mycobacterium marinum. Finally, we provide insight into as to why members of the Mmuc- and Mneo-clades show resistance to rifampin and isoniazid, and why MmucT forms a rough colony morphotype.

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Mycobacterium mucogenicum and Mycobacterium neoaurum Bloodstream Infection in Immunocompromised Hosts

10.21203/rs.3.rs-29851/v1 ◽

2020 ◽

Author(s):

Armelle Pérez-Cortés Villalobos ◽

Coleman Rotstein

Keyword(s):

Bloodstream Infection ◽

Vascular Access ◽

Antimicrobial Therapy ◽

Injection Drug Users ◽

Drug Users ◽

Retrospective Chart Review ◽

Vascular Access Devices ◽

Mycobacterium Neoaurum ◽

Immunocompromised Hosts ◽

Mycobacterium Mucogenicum

Abstract Background Mycobacterium mucogenicum and Mycobacterium neoaurum are an infrequent cause of bloodstream infection in humans. When they are isolated from blood, it is not unusual for them to be considered to be contaminants initially. Methods A retrospective chart review of patients diagnosed with M. mucogenicum and M. neoaurum bacteremia was conducted from January 1998 to December 2018 at the University Health Network, Toronto, Canada. Results We identified 28 patients who had bloodstream infection caused by these organisms. These patients were immunocompromised hosts, patients with chronically indwelling vascular access devices or injection drug users. In 28.5% of the patients the isolation of these mycobacteria was considered colonization, 82% of the cases received antimicrobial therapy and in 95% of the patients the central line was removed. Conclusions The removal of the vascular access device and treatment with either single or combination antimicrobial therapy produced successful outcomes for these infections.

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Innate immune responses and type 1 diabetes: A role for a long non-coding RNA?

Diabetologie und Stoffwechsel ◽

10.1055/s-0034-1374906 ◽

2014 ◽

Vol 9 (S 01) ◽

Author(s):

MP Ashton ◽

I Tan ◽

L Mackin ◽

C Elso ◽

E Chu ◽

...

Keyword(s):

Type 1 Diabetes ◽

Immune Responses ◽

Innate Immune ◽

Innate Immune Responses ◽

Non Coding Rna ◽

Long Non Coding Rna

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Long non-coding RNA CCAT1 is overexpressed in endometrial cancer and regulates growth and transcriptome of endometrial adenocarcinoma cells

10.1055/s-0040-1718183 ◽

2020 ◽

Author(s):

S Schüler-Toprak ◽

M Skrzypczak ◽

F Weber ◽

O Ortmann ◽

O Treeck

Keyword(s):

Endometrial Cancer ◽

Endometrial Adenocarcinoma ◽

Non Coding Rna ◽

Adenocarcinoma Cells ◽

Long Non Coding Rna

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Long non-coding RNA expression profiles in human parathyroid tumors

Endocrine Abstracts ◽

10.1530/endoabs.49.gp130 ◽

2017 ◽

Author(s):

Annamaria Morotti ◽

Irene Forno ◽

Valentina Andre ◽

Andrea Terrasi ◽

Chiara Verdelli ◽

...

Keyword(s):

Expression Profiles ◽

Rna Expression ◽

Parathyroid Tumors ◽

Non Coding Rna ◽

Long Non Coding Rna

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The Variant rs145204276 of GAS5 is Associated with the Development and Prognosis of Gastric Cancer

Journal of Gastrointestinal and Liver Diseases ◽

10.15403/jgld.2014.1121.271.qjl ◽

2018 ◽

Vol 27 (1) ◽

pp. 19-24 ◽

Cited By ~ 7

Author(s):

Qianjun Li ◽

Gang Ma ◽

Huimin Guo ◽

Suhua Sun ◽

Ying Xu ◽

...

Keyword(s):

Gastric Cancer ◽

Survival Rate ◽

Cancer Progression ◽

Regulatory Mechanism ◽

Tumor Stage ◽

Kaplan Meier ◽

Non Coding Rna ◽

Early Tumor ◽

Long Non Coding Rna ◽

Clinicopathological Variables

Background & Aims: Down-regulation of the growth arrest specific transcript 5 (GAS5) (long non-coding RNA) is associated with cell proliferation of gastric cancer (GC) and a poor prognosis. We aimed to investigate whether the variant rs145204276 of GAS5 is associated with the prognosis of GC in the Chinese population, and to unveil the regulatory mechanism underlying the GAS5 expression in GC tissues.Method: 1,253 GC patients and 1,354 healthy controls were included. The frequency of the genotype del/del and the allele del of rs145204276 were compared between the patients and the controls and between different subgroups of patients classified by clinicopathological variables. The overall survival rate was analyzed according to the Kaplan-Meier method using the log-rank test.Results: The frequency of genotype del/del was significantly lower in patients than in the controls (7.0% vs. 9.1%, p = 0.001). Kaplan-Meier analysis showed that genotype del/del was significantly associated with a higher survival rate (p = 0.01). Patients with late tumor stage were found to have a significantly lower rate of genotype del/del than those with an early tumor stage (4.9% vs. 8.8%, p = 0.01). Patients with UICC III and IV were found to have a significantly lower rate of genotype del/del than those with UICC I and II (5.3% vs. 8.1%, p = 0.02).Conclusion: The variant rs145204276 of GAS5 is associated with the development and prognosis of GC. The allele del of rs145204276 is associated with a remarkably lower incidence of cancer progression and metastasis.

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Impact of microRNAs on molecular epidemiology

Orvosi Hetilap ◽

10.1556/oh.2011.29066 ◽

2011 ◽

Vol 152 (16) ◽

pp. 633-641 ◽

Cited By ~ 2

Author(s):

Katalin Gőcze ◽

Katalin Gombos ◽

Gábor Pajkos ◽

Ingrid Magda ◽

Ágoston Ember ◽

...

Keyword(s):

Functional Analysis ◽

Cancer Research ◽

Molecular Epidemiology ◽

Wide Spectrum ◽

Translation Regulation ◽

Rna Sequences ◽

Serum Mirna ◽

The Past ◽

Non Coding Rna ◽

Free Serum

Cancer research concerning short non-coding RNA sequences and functionally linked to RNA interference (RNAi) have reached explosive breakthrough in the past decade. Molecular technology applies microRNA in extremely wide spectrum from molecular tumor prediction, diagnostics, progression monitoring and prevention. Functional analysis of tissue miRNA and cell-free serum miRNA in posttranscription and translation regulation innovated and restructured the knowledge on the field. This review focuses on molecular epidemiology and primary prevention aspects of the small non-coding RNA sequences. Orv. Hetil., 2011, 152, 633–641.

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