scholarly journals Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Rapid Identification ◽  
Identification Accuracy ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract Background Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. Objective To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. Methods Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. Results The identification accuracy of MALDI-TOF MS was 84% for species (I2 = 98.0%, P < 0.1), and 92% for genus (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95–97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs. 86% for MALDI biotyper system. Conclusions Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.

scholarly journals Application analysis of MALDI-TOF MS in rapid identification of anaerobic bacteria

2019 ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Identification Accuracy ◽  
P Value ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in the clinical laboratories with high accuracy in the identification of microorganisms. METHOD: This study was designed to evaluate MALDI-TOF MS for identification of clinical pathogenic anaerobes. RESULT: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. Results shown that the identification accuracy of MALDI-TOF MS at species was 84% (I2 = 98.0%, P < 0.1), genus was 92% (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95% to 97%. Next were Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was lower. Meanwhile, the overall capabilitys of two MALDI-TOF MS systems were different. The identification accuracy rate of VITEK MS was 90%, compared to 86% by the MALDI biotyper system. CONCLUSON: In summary, our research showed that MALDI-TOF-MS was satisfactory in the identification of genus in clinical pathogenic anaerobic bacteria. However, this method still suffered from different drawbacks in the identification of the rare anaerobes and species levels of common anaerobic bacteria.

scholarly journals Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

2019 ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Identification Accuracy ◽  
P Value ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSIONS: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

scholarly journals Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

2019 ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Identification Accuracy ◽  
P Value ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSIONS: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

scholarly journals Application of MALDI-TOF MS to rapid identification of anaerobic bacteria: a meta-analysis

2019 ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Identification Accuracy ◽  
P Value ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS: The identification accuracy of MALDI-TOF MS was 84% for species (I2 = 98.0%, P < 0.1), and 92% for genus (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs. 86% for MALDI biotyper system. CONCLUSION: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffered from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.

scholarly journals Application of MALDI-TOF MS to rapid identification of anaerobic bacteria: a meta-analysis

2019 ◽  
Author(s):  
Ying Li ◽  
Mingzhu Shan ◽  
Zuobin Zhu ◽  
Xuhua Mao ◽  
Mingju Yan ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Meta Analysis ◽  
Anaerobic Bacteria ◽  
Identification Accuracy ◽  
P Value ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Analytical Technique ◽  
Maldi Tof ◽  
Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSION: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI-TOF-MS

2008 ◽  
Vol 23 (5) ◽  
pp. 372-376 ◽  
Author(s):  
C. S. Stîngu ◽  
A. C. Rodloff ◽  
H. Jentsch ◽  
R. Schaumann ◽  
K. Eschrich
Keyword(s):  
Anaerobic Bacteria ◽  
Rapid Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Direct MALDI-TOF MS Testing from Positive Blood Cultures for Rapid Identification of Bloodstream Infection-Causing Anaerobic Bacteria

2021 ◽  
Author(s):  
Tiziana D’Inzeo ◽  
Barbara Fiori ◽  
Flora Marzia Liotti ◽  
Venere Cortazzo ◽  
Debora Talamonti ◽  
...  
Keyword(s):  
Bloodstream Infection ◽  
Anaerobic Bacteria ◽  
Rapid Identification ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Direct Use ◽  
Tof Ms

Direct use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) on positive blood culture (PBC) bottles may accelerate the identification of bloodstream infection (BSI) causing organisms (1), particularly anaerobic bacteria for which BSI-associated mortality rates are high (2).…

scholarly journals Evaluation of MALDI-TOF Mass Spectrometry and Sepsityper Kit™ for the Direct Identification of Organisms from Sterile Body Fluids in a Canadian Pediatric Hospital

2013 ◽  
Vol 24 (4) ◽  
pp. 191-194 ◽  
Author(s):  
Manal Tadros ◽  
Astrid Petrich
Keyword(s):  
Mass Spectrometry ◽  
Blood Culture ◽  
Software Tool ◽  
Rapid Identification ◽  
Correct Identification ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) can be used to identify bacteria directly from positive blood and sterile fluid cultures. The authors evaluated a commercially available kit – the Sepsityper Kit (Bruker Daltonik, Germany) – and MALDI-TOF MS for the rapid identification of organisms from 80 flagged positive blood culture broths, of which 73 (91.2%) were blood culture specimens and seven (8.7%) were cerebrospinal fluid specimens, in comparison with conventional identification methods. Correct identification to the genus and species levels was obtained in 75 of 80 (93.8%) and 39 of 50 (78%) blood culture broths, respectively. Applying the blood culture analysis module, a newly developed software tool, improved the species identification of Gram-negative organisms from 94.7% to 100% and of Gram-positive organisms from 66.7% to 70%.MALDI-TOF MS is a promising tool for the direct identification of organisms cultured from sterile sites.

scholarly journals Accuracy of matrix-assisted LASER desorption ionization–time of flight mass spectrometry for identification of Candida

2019 ◽  
Vol 39 (10) ◽  
Author(s):  
Tian-Ao Xie ◽  
Ye-Ling Liu ◽  
Chuan Liang ◽  
Yuan-Yuan Huang ◽  
Jin-Wei Li ◽  
...  
Keyword(s):  
Pooled Analysis ◽  
Diagnostic Methods ◽  
Identification Accuracy ◽  
Cochrane Library ◽  
Original Research ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Abstract Background: Candida is a fungus that causes various types of candidemia, which is the fourth major infectious disease of the blood system. MALDI-TOF-MS is a simple and rapid detection instrument. The aim of the present study was to verify the accuracy of MALDI-TOF-MS in detecting Candida. Method: A pooled analysis of articles on MALDI-TOF-MS for diagnosis of candidemia was performed. The quality of original research was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) guidelines. Stata 12.0 software was used to merge the correct identification rates of Candida and Candida subspecies and obtain pooled sensitivity and specificity of the diagnostic methods. Heterogeneity was found in the subgroup analysis of the included articles. Hence, we explored the factors causing the heterogeneity and its impact on the overall situation. Sensitivity analysis was used to examine the effect of Candida level on total response. Egger’s test was used to evaluate the publication bias of the included articles. Results: A total of 16 articles in Pubmed, 79 articles in Embase, 1 article in Cochrane Library, 30 articles in Web of Science and 3 from other sources were identified, of which 10 articles were included based on the inclusion and exclusion criteria. The overall identification accuracy was 100%. Conclusion: The accuracy of MALDI-TOF-MS for the identification of Candida was 100%. Further research is necessary to determine whether MALDI-TOF-MS can be used as a clinical diagnostic standard for the identification of Candida.

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