Application of MALDI-TOF MS to rapid identification of anaerobic bacteria: a meta-analysis

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS: The identification accuracy of MALDI-TOF MS was 84% for species (I2 = 98.0%, P < 0.1), and 92% for genus (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs. 86% for MALDI biotyper system. CONCLUSION: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffered from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.

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Application analysis of MALDI-TOF MS in rapid identification of anaerobic bacteria

10.21203/rs.2.9661/v1 ◽

2019 ◽

Author(s):

Ying Li ◽

Mingzhu Shan ◽

Zuobin Zhu ◽

Xuhua Mao ◽

Mingju Yan ◽

...

Keyword(s):

Fixed Effects ◽

Meta Analysis ◽

Anaerobic Bacteria ◽

Identification Accuracy ◽

P Value ◽

Correct Identification ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in the clinical laboratories with high accuracy in the identification of microorganisms. METHOD: This study was designed to evaluate MALDI-TOF MS for identification of clinical pathogenic anaerobes. RESULT: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. Results shown that the identification accuracy of MALDI-TOF MS at species was 84% (I2 = 98.0%, P < 0.1), genus was 92% (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95% to 97%. Next were Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was lower. Meanwhile, the overall capabilitys of two MALDI-TOF MS systems were different. The identification accuracy rate of VITEK MS was 90%, compared to 86% by the MALDI biotyper system. CONCLUSON: In summary, our research showed that MALDI-TOF-MS was satisfactory in the identification of genus in clinical pathogenic anaerobic bacteria. However, this method still suffered from different drawbacks in the identification of the rare anaerobes and species levels of common anaerobic bacteria.

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Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

10.21203/rs.2.9661/v5 ◽

2019 ◽

Author(s):

Ying Li ◽

Mingzhu Shan ◽

Zuobin Zhu ◽

Xuhua Mao ◽

Mingju Yan ◽

...

Keyword(s):

Fixed Effects ◽

Meta Analysis ◽

Anaerobic Bacteria ◽

Identification Accuracy ◽

P Value ◽

Correct Identification ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Tof Ms

Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSIONS: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

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Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

10.21203/rs.2.9661/v4 ◽

2019 ◽

Author(s):

Ying Li ◽

Mingzhu Shan ◽

Zuobin Zhu ◽

Xuhua Mao ◽

Mingju Yan ◽

...

Keyword(s):

Fixed Effects ◽

Meta Analysis ◽

Anaerobic Bacteria ◽

Identification Accuracy ◽

P Value ◽

Correct Identification ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSIONS: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

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Application of MALDI-TOF MS to rapid identification of anaerobic bacteria: a meta-analysis

10.21203/rs.2.9661/v3 ◽

2019 ◽

Author(s):

Ying Li ◽

Mingzhu Shan ◽

Zuobin Zhu ◽

Xuhua Mao ◽

Mingju Yan ◽

...

Keyword(s):

Fixed Effects ◽

Meta Analysis ◽

Anaerobic Bacteria ◽

Identification Accuracy ◽

P Value ◽

Correct Identification ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Tof Ms

Abstract BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system. CONCLUSION: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. Key words : MALDI-TOF MS , anaerobec, bacteria identification

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Application of MALDI-TOF MS to rapid identification of anaerobic bacteria

BMC Infectious Diseases ◽

10.1186/s12879-019-4584-0 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 6

Author(s):

Ying Li ◽

Mingzhu Shan ◽

Zuobin Zhu ◽

Xuhua Mao ◽

Mingju Yan ◽

...

Keyword(s):

Fixed Effects ◽

Meta Analysis ◽

Anaerobic Bacteria ◽

Rapid Identification ◽

Identification Accuracy ◽

Correct Identification ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Tof Ms

Abstract Background Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. Objective To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. Methods Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. Results The identification accuracy of MALDI-TOF MS was 84% for species (I2 = 98.0%, P < 0.1), and 92% for genus (I2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95–97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs. 86% for MALDI biotyper system. Conclusions Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.

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Accuracy of matrix-assisted LASER desorption ionization–time of flight mass spectrometry for identification of Candida

Bioscience Reports ◽

10.1042/bsr20190859 ◽

2019 ◽

Vol 39 (10) ◽

Cited By ~ 1

Author(s):

Tian-Ao Xie ◽

Ye-Ling Liu ◽

Chuan Liang ◽

Yuan-Yuan Huang ◽

Jin-Wei Li ◽

...

Keyword(s):

Pooled Analysis ◽

Diagnostic Methods ◽

Identification Accuracy ◽

Cochrane Library ◽

Original Research ◽

Correct Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Tof Ms

Abstract Background: Candida is a fungus that causes various types of candidemia, which is the fourth major infectious disease of the blood system. MALDI-TOF-MS is a simple and rapid detection instrument. The aim of the present study was to verify the accuracy of MALDI-TOF-MS in detecting Candida. Method: A pooled analysis of articles on MALDI-TOF-MS for diagnosis of candidemia was performed. The quality of original research was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) guidelines. Stata 12.0 software was used to merge the correct identification rates of Candida and Candida subspecies and obtain pooled sensitivity and specificity of the diagnostic methods. Heterogeneity was found in the subgroup analysis of the included articles. Hence, we explored the factors causing the heterogeneity and its impact on the overall situation. Sensitivity analysis was used to examine the effect of Candida level on total response. Egger’s test was used to evaluate the publication bias of the included articles. Results: A total of 16 articles in Pubmed, 79 articles in Embase, 1 article in Cochrane Library, 30 articles in Web of Science and 3 from other sources were identified, of which 10 articles were included based on the inclusion and exclusion criteria. The overall identification accuracy was 100%. Conclusion: The accuracy of MALDI-TOF-MS for the identification of Candida was 100%. Further research is necessary to determine whether MALDI-TOF-MS can be used as a clinical diagnostic standard for the identification of Candida.

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Diagnostic accuracy of MALDI-TOF mass spectrometry for the direct identification of clinical pathogens from urine

Open Medicine ◽

10.1515/med-2020-0038 ◽

2020 ◽

Vol 15 (1) ◽

pp. 266-273

Author(s):

Min Tang ◽

Jia Yang ◽

Ying Li ◽

Luhua Zhang ◽

Ying Peng ◽

...

Keyword(s):

Mass Spectrometry ◽

Likelihood Ratio ◽

Meta Analysis ◽

Positive Likelihood Ratio ◽

Negative Likelihood Ratio ◽

Urine Samples ◽

Direct Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

AbstractMatrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has become one of the most popular methods for the rapid and cost-effective detection of clinical pathogenic microorganisms. This study aimed to evaluate and compare the diagnostic performance of MALDI-TOF MS with that of conventional approaches for the direct identification of pathogens from urine samples. A systematic review was conducted based on a literature search of relevant databases. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR) and area under the summary receiver operating characteristic (SROC) curve of the combined studies were estimated. Nine studies with a total of 3920 subjects were considered eligible and included in the meta-analysis. The pooled sensitivity was 0.85 (95% CI 0.79-0.90), and the pooled specificity was 0.93 (95% CI 0.82-0.97). The PLR and NLR were 11.51 (95% CI 4.53-29.26) and 0.16 (95% CI 0.11-0.24), respectively. The area under the SROC curve was 0.93 (95% CI 0.91-0.95). Sensitivity analysis showed that the results of this meta-analysis were stable. MALDI-TOF MS could directly identify microorganisms from urine samples with high sensitivity and specificity.

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Identification of Lice Stored in Alcohol Using MALDI-TOF MS

Journal of Medical Entomology ◽

10.1093/jme/tjaa266 ◽

2020 ◽

Author(s):

Hanene Benyahia ◽

Basma Ouarti ◽

Adama Zan Diarra ◽

Mehdi Boucheikhchoukh ◽

Mohamed Nadir Meguini ◽

...

Keyword(s):

Economic Consequences ◽

Correct Identification ◽

Protein Profiles ◽

Innovative Strategy ◽

Maldi Tof Ms ◽

Pediculus Humanus ◽

Maldi Tof ◽

Ms Analysis ◽

Storage Methods ◽

Tof Ms

Abstract Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

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Detection of Ovarian Cancer Using Samples Sourced from the Vaginal Microenvironment

10.1101/597740 ◽

2019 ◽

Author(s):

Melissa M. Galey ◽

Alexandria N. Young ◽

Valentina Z. Petukhova ◽

Mingxun Wang ◽

Jian Wang ◽

...

Keyword(s):

Ovarian Cancer ◽

Disease Progression ◽

Xenograft Model ◽

Screening Methods ◽

Maldi Tof Ms ◽

Analytical Technique ◽

Maldi Tof ◽

Specificity And Sensitivity ◽

Biological Mixtures ◽

Tof Ms

AbstractMass spectrometry (MS) offers high levels of specificity and sensitivity in clinical applications, and we have previously been able to demonstrate that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS is capable of distinguishing two-component biological mixtures at low limits of detection. Ovarian cancer is notoriously difficult to detect due to the lack of any screening methods for early detection. By sampling a local microenvironment, such as the vaginal fluids, a MS based method is presented that was capable of monitoring disease progression from vaginally collected, local samples from tumor bearing mice. A murine xenograft model of high grade serous ovarian carcinoma (HGSOC) was used for this study and vaginal lavages were obtained from mice on a weekly basis throughout disease progression and subjected to our MALDI-TOF MS workflow followed by statistical analyses. Proteins in the 4-20 kDa region of the mass spectrum could consistently be measured to yield a fingerprint that correlated with disease progression over time. These fingerprints were found to be statistically stable across all mice with the protein fingerprint converging towards the end point of the study. MALDI-TOF MS serves as a unique analytical technique for measuring a sampled vaginal microenvironment in a specific and sensitive manner for the detection of HGSOC in a murine model.

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Black aspergilli: A remaining challenge in fungal taxonomy?

Medical Mycology ◽

10.1093/mmy/myy124 ◽

2018 ◽

Vol 57 (6) ◽

pp. 773-780 ◽

Cited By ~ 8

Author(s):

Elizabet D’hooge ◽

Pierre Becker ◽

Dirk Stubbe ◽

Anne-Cécile Normand ◽

Renaud Piarroux ◽

...

Keyword(s):

Antifungal Susceptibility ◽

Identification Accuracy ◽

Clinical Isolates ◽

Reference Database ◽

Data Set ◽

Ionization Time ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Tof Ms

AbstractAspergillus section Nigri is a taxonomically difficult but medically and economically important group. In this study, an update of the taxonomy of A. section Nigri strains within the BCCM/IHEM collection has been conducted. The identification accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was tested and the antifungal susceptibilities of clinical isolates were evaluated. A total of 175 strains were molecularly analyzed. Three regions were amplified (ITS, benA, and caM) and a multi-locus phylogeny of the combined loci was created by using maximum likelihood analysis. The in-house MALDI-TOF MS reference database was extended and an identification data set of 135 strains was run against a reference data set. Antifungal susceptibility was tested for voriconazole, itraconazole, and amphotericin B, using the EUCAST method. Phylogenetic analysis revealed 18 species in our data set. MALDI-TOF MS was able to distinguish between A. brasiliensis, A. brunneoviolaceus, A. neoniger, A. niger, A. tubingensis, and A. welwitschiae of A. sect. Nigri. In the routine clinical lab, isolates of A. sect. Nigri are often identified as A. niger. However, in the clinical isolates of our data set, A. tubingensis (n = 35) and A. welwitschiae (n = 34) are more common than A. niger (n = 9). Decreased antifungal susceptibility to azoles was observed in clinical isolates of the /tubingensis clade. This emphasizes the importance of identification up to species level or at least up to clade level in the clinical lab. Our results indicate that MALDI-TOF MS can be a powerful tool to replace classical morphology.

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