scholarly journals Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Reda M. El-Shishtawy ◽  
Saleh A. Mohamed ◽  
Abdullah M. Asiri ◽  
Abu-bakr M. Gomaa ◽  
Ibrahim H. Ibrahim ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Trichoderma Virens

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 4982-4995
Author(s):  
Mei-Ling Han ◽  
Qi An ◽  
Sai-Fei He ◽  
Xiao-Lin Zhang ◽  
Ming-Hui Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Wild Strain ◽  
Xylanase Activity ◽  
Lignocellulosic Materials ◽  
Lignocellulolytic Enzymes ◽  
Poplar Sawdust

Solid state fermentation with different lignocellulolytic materials as inducers was used for lignocellulolytic enzyme production in this study. Pleurotus ostreatus strains were assessed by measuring laccase, CMCase, and xylanase activities. The secretion potential of the lignocellulolytic enzymes by wild and cultivated strains was analyzed for the first time. The wild and cultivated strain showed their unique capacities for secreting lignocellulolytic enzymes on solid-state fermentation with different lignocellulosic materials. The wild P. ostreatus strain preferred corncob for the secretion of laccase and xylanase activity, but the cultivated strain preferred poplar sawdust. The wild strain and cultivated strain showed a consistent preference for poplar sawdust for the secretion of CMCase activity. The wild strain was advantageous because it achieved the maximum hydrolytic enzyme activities within a short time period. Poplar sawdust and corncob were conducive to laccase secretion by the wild or cultivated strains and the rapid accumulation of laccase on solid-state fermentation. Additionally, continuous, stable laccase production was an extremely important advantage by solid-state fermentation of poplar sawdust, particularly in the wild strain. These findings are helpful in selecting the appropriate strain that corresponds to suitable lignocellulosic materials. The optimization of integrated industrial lignocellulolytic enzyme production can also be achieved.

scholarly journals Exo-polygalacturonase production by Penicillium roqueforti on pumpkin oil cake in solid state fermentation

2007 ◽  
pp. 127-137 ◽  
Author(s):  
Draginja Pericin ◽  
Senka Madjarev ◽  
Ljiljana Radulovic ◽  
Marija Skrinjar
Keyword(s):  
Solid State ◽  
Water Activity ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Starter Culture ◽  
Penicillium Roqueforti ◽  
Initial Water ◽  
Fermentation Time ◽  
Pectolytic Activity

The feasibility of using pumpkin oil cake (PuOC), individual and in combination with wheat bran (WB), as substrate for the production of Exo-polygalacturonase (Exo-p) by starter culture Penicillium roqueforti in solid state fermentation (SSF) has been evaluated. The kinetics of enzyme production was investigated using PuOC alone in the range from 13 to 168 h, with moisture contents varying from 44% the ability to grow and produce Exo-p activity on this substrate, reaching a maximum value of 1451.75 U/g.d.w PuOC by the 5th day of fermentation. Fermentation experiments indicated that the water activity (aw) influenced the enzyme production. A medium with aw 0.932 and the fermentation time of 5 days were selected, as these conditions resulted in the highest pectolytic activity and were used for further investigation. A next step in this research was to examine the effect of the substrate combination, PuOC with wheat bran (WB), in different ratios. The addition of WB as carbon sources was found to have a significant influence on the enzymes yields. Exo-p activities were the highest with initial water activity of a w 0.932 and PuOC supplementation with WB (1:0.67).

Hydrolytic enzyme production in solid-state fermentation by Aspergillus niger 3T5B8

2000 ◽  
Vol 36 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sonia Couri ◽  
Selma da Costa Terzi ◽  
Gustavo A Saavedra Pinto ◽  
Suely Pereira Freitas ◽  
Antonio Carlos Augusto da Costa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme

scholarly journals Trichoderma Reesei Cellulase Produced by Submerged Versus Solid State Fermentations

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Diana NEAGU ◽  
Jacqueline DESTAIN ◽  
Phillipe THONART ◽  
Carmen SOCACIU
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Carbon Sources ◽  
Production Yield ◽  
Trichoderma Sp ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher. 

Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition

2020 ◽  
pp. 103159
Author(s):  
Sonja Jakovetić Tanasković ◽  
Nataša Šekuljica ◽  
Jelena Jovanović ◽  
Ivana Gazikalović ◽  
Sanja Grbavčić ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Food Component ◽  
Nutritional Factors
Sign in / Sign up

Export Citation Format

Share Document