scholarly journals Trichoderma Reesei Cellulase Produced by Submerged Versus Solid State Fermentations

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Diana NEAGU ◽  
Jacqueline DESTAIN ◽  
Phillipe THONART ◽  
Carmen SOCACIU
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Carbon Sources ◽  
Production Yield ◽  
Trichoderma Sp ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher. 

scholarly journals Improvement of Amylase Production by UV Mutagenesis of Aspergillus flavus FSS63 under Solid State Fermentation

2016 ◽  
Vol 59 (2) ◽  
pp. 80-84
Author(s):  
Yasser Bakri ◽  
Samir Elkhouri
Keyword(s):  
Solid State ◽  
Aspergillus Flavus ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Physical Parameters ◽  
Uv Mutagenesis ◽  
Amylase Production ◽  
Optimum Ph ◽  
Uvc Radiation ◽  
Optimum Ph And Temperature

Enhancement of the amylase productivity by Aspergillus flavus was investigated. Spores ofstrain were exposed to ultraviolet (UVC) radiation and 10 different mutants were selected and isolatedfrom starch plate agar on the basis of the visible clearance zone around the colonies. The amylase productionby selected mutants was evaluated under solid state fermentation. One mutant of A. flavus FSS63UV8showed higher biosynthesis level of amylase (733 IU/g), which was 3.35 fold higher than that detectedin the parental strain. Physical parameters optimisation revealed that the optimum pH and temperature foramylase production obtained by mutant are 7.0 and 35°C, respectively. Among several tested agriculturalwastes , wheat bran was found to support the highest yield of amylase after 5 days of incubation. A. flavusFSS63UV8 strain proved to be a promising microorganism for a high amylase production in a simplemedium.

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Optimization of Conditions for Xylanase Production Using Aspergillus tubingensis Under Different Carbon Sources

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Isiaka Kolade ◽  
Kamoldeen Ajijolakewu
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Rice Husk ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Xylanase Activity ◽  
Aspergillus Tubingensis ◽  
Xylanase Production ◽  
Corn Cobs

Xylanases are hydrolytic enzymes with wide range of applications in food processing, bleaching of pulp in paper manufacturing industry, bio-conversion of biomass wastes to fermentable sugars and enhancing nutrient digestibility in animal feeds. The optimization of growth conditions and evaluation of an appropriate substrate as carbon source among cassava peels, corn cobs, wheat bran and rice husk on xylanase production by novel strain of Aspergillus tubingensis under Solid State Fermentation (SSF) was investigated. The fungal isolate was identified based on ribosomal RNA gene and ITS gene sequencing analysis as Aspergillus tubingensis. Results showed that Corn cobs had the highest xylanase production among the four substrates. Corn cobs recorded the highest value of xylanase production at pH of 6.0 (107.97 U/g), after incubation period of 72 hour (111.23 U/g), at temperature of 30oC (44.26 U/g) and at ratio 1:3 (45.68 U/g). The optimum growth conditions for xylanase production by Aspergillus tubingensis were: pH 6.0, incubation period of 72 hours, temperature of 30oC and substrate concentration of 1:3 (4g of substrate per 12ml of fermentation medium). Corn cobs showed the highest yield of xylanase activity (111.23±0.31 U/g), followed by Rice husk (101.91±0.72 U/g), Wheat bran (89.30±1.16 U/g) and Cassava peel (87.03±0.57 U/g). In conclusion, among the various agro residues that were used as carbon sources, Corn cobs had maximum xylanase activity. Various culture conditions were optimized by using one factor at a time method and the maximum xylanase production was obtained at pH of 6.0, incubation period of 72 hour, temperature of 30oC and substrate concentration of 1:3 under solid state fermentation. It is therefore suggested that some other notable environmental and fermentation factors that influence metabolism-mediated production yields of xylanase like aeration, agitation, carbon and nitrogen sources, metal ion requirement,  inoculum size etc. should be optimized for maximum production of enzyme.   Keywords: Solid State Fermentation, Cassava peels, Corn cobs, Wheat bran, Rice husk

scholarly journals Exo-polygalacturonase production by Penicillium roqueforti on pumpkin oil cake in solid state fermentation

2007 ◽  
pp. 127-137 ◽  
Author(s):  
Draginja Pericin ◽  
Senka Madjarev ◽  
Ljiljana Radulovic ◽  
Marija Skrinjar
Keyword(s):  
Solid State ◽  
Water Activity ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Starter Culture ◽  
Penicillium Roqueforti ◽  
Initial Water ◽  
Fermentation Time ◽  
Pectolytic Activity

The feasibility of using pumpkin oil cake (PuOC), individual and in combination with wheat bran (WB), as substrate for the production of Exo-polygalacturonase (Exo-p) by starter culture Penicillium roqueforti in solid state fermentation (SSF) has been evaluated. The kinetics of enzyme production was investigated using PuOC alone in the range from 13 to 168 h, with moisture contents varying from 44% the ability to grow and produce Exo-p activity on this substrate, reaching a maximum value of 1451.75 U/g.d.w PuOC by the 5th day of fermentation. Fermentation experiments indicated that the water activity (aw) influenced the enzyme production. A medium with aw 0.932 and the fermentation time of 5 days were selected, as these conditions resulted in the highest pectolytic activity and were used for further investigation. A next step in this research was to examine the effect of the substrate combination, PuOC with wheat bran (WB), in different ratios. The addition of WB as carbon sources was found to have a significant influence on the enzymes yields. Exo-p activities were the highest with initial water activity of a w 0.932 and PuOC supplementation with WB (1:0.67).

scholarly journals Statistical Optimization of Media Components for Xylanase Production by Aspergillus spp. Using Solid State Fermentation and its Application in Fruit Juice Clarification

2021 ◽  
pp. 151-166
Author(s):  
Laxmikant R. Patil ◽  
Anil R. Shet ◽  
Sharanappa Achappa ◽  
Shivalingsarj V. Desai ◽  
Veeranna S. Hombalimath ◽  
...  
Keyword(s):  
Solid State ◽  
Yeast Extract ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Gel Filtration ◽  
Partial Purification ◽  
Elution Time ◽  
Xylanase Production ◽  
Juice Clarification

Xylanases are enzymes that convert xylan into xylose, xylobiose, and xylotriose. The present study deals with the production and optimization of xylanase through Solid-State Fermentation (SSF) using different agricultural wastes by   Aspergillus spp. The Plackett Burman (PB) design was used to screen significant media components affecting the xylanase production. The carbon sources screened were wheat bran, rice bran, sugarcane bagasse, corn cob, and orange peel. The nitrogen sources screened were yeast extract, peptone, (NH4)2SO4, Na2NO3, and urea. Also, nine different salts such as KCl, MgSO4, Na2HPO4, CaCl2, FeSO4, ZnSO4, Na2CO3, KH2PO4, and NaH2PO4 which act as trace elements were screened. The results showed that wheat bran, yeast extract, Na2NO3 and KCl are the significant factors that affect xylanase production. A 33 Full Factorial Design (FFD) was performed to optimize the significant media components (wheat bran, KCl, yeast extract) obtained from PB design using Response Surface Methodology (RSM). Statistical analysis of results showed that wheat bran, KCl, yeast extract, and interaction between wheat bran and yeast extract were found to be significant. The optimum concentration of wheat bran, KCl, yeast extract was 8 g/L, 0.1 g/L and 3 g/L. The Partial purification of xylanase was carried out using ammonium salt precipitation and dialysis. Gel filtration chromatography was performed to optimize the elution time, which was found to be 6 minutes. Application of xylanase in orange juice clarification was studied at 40 °C, 50 °C, and 60 °C. The optimum temperature obtained was 60 ºC.

scholarly journals Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Reda M. El-Shishtawy ◽  
Saleh A. Mohamed ◽  
Abdullah M. Asiri ◽  
Abu-bakr M. Gomaa ◽  
Ibrahim H. Ibrahim ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Trichoderma Virens

scholarly journals A Comparative Study of New Aspergillus Strains for Proteolytic Enzymes Production by Solid State Fermentation

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Gastón Ezequiel Ortiz ◽  
Diego Gabriel Noseda ◽  
María Clara Ponce Mora ◽  
Matías Nicolás Recupero ◽  
Martín Blasco ◽  
...  
Keyword(s):  
Solid State ◽  
Comparative Study ◽  
Solid State Fermentation ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Thermodynamics And Kinetics ◽  
Wide Range ◽  
Optimum Ph ◽  
Hydrolysis Of ◽  
Optimum Ph And Temperature

A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease activity. Biochemical, thermodynamics, and kinetics features such as optimum pH and temperature values, thermal stability, activation energy (Ea), quotient energy (Q10), Km, and Vmax were studied in four enzymatic extracts from the selected strains that showed the highest productivity. Additionally, these strains were evaluated by zymogram analysis obtaining protease profiles with a wide range of molecular weight for each sample. From these four strains with the highest productivity, the proteolytic extract of A. sojae ATCC 20235 was shown to be an appropriate biocatalyst for hydrolysis of casein and gelatin substrates, increasing its antioxidant activities in 35% and 125%, respectively.

scholarly journals Isolation, screening of Aspergillus flavus and its production parameters for á- amylase under solid state fermentation

2011 ◽  
Vol 3 (2) ◽  
pp. 268-273
Author(s):  
K. N. Geetha ◽  
K. Jeyaprakash ◽  
Y. P. Nagaraja
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Incubation Temperature ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Solid Substrate ◽  
Fungal Species

The amylase producing fungi were isolated from spoiled fruits, vegetables and soil, in and around Bangalore, Karnataka, India. The isolates were identified and five fungal species were screened. The best amylase producer among them, Aspergillus sp was selected for enzyme production by both sub merged fermentation using mineral salt medium (MSM) and solid state fermentations using wheat bran as a solid substrate. The various parameters influencing solid state fermentation were optimized. The most important factors are such as pH, incubation temperature, incubation period, carbon sources, nitrogen sources and moisture content. The maximum amount of enzyme production was obtained when solid state fermentation was carried out with soluble starch as carbon source and beef extract (1% each) as nitrogen source, optimum conditions of pH 7.0, an incubation temperature of 25 (±2) °C, incubation time 96 h and 62% moisture content.

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