scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Pcr Rflp ◽  
Flotation Technique

Abstract Background Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb. Methods From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis. The isolated genotyped was type II. Besides, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Besides, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii -specific PCR. The brain samples were investigated by T. gondii -specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma -like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T . gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Beside, eight Toxoplasma- positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Beside, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in Mashhad suburb . Methods: From April 2016 to August 2017, in this study, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii -specific PCR. The brain samples were investigated by T. gondii -specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated were genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers . Results: In the present study, oocysts-like Toxoplasma were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Beside, eight Toxoplasma- positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

Evidence of the three main clonalToxoplasma gondiilineages from wild mammalian carnivores in the UK

Parasitology ◽  
2013 ◽  
Vol 140 (14) ◽  
pp. 1768-1776 ◽  
Author(s):  
A. BURRELLS ◽  
P. M. BARTLEY ◽  
I. A. ZIMMER ◽  
S. ROY ◽  
A. C. KITCHENER ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rflp Markers ◽  
Type I ◽  
Type Ii ◽  
Red Foxes ◽  
Mammal Species ◽  
Tissue Samples ◽  
Pcr Rflp ◽  
Few Data ◽  
The Uk

SUMMARYToxoplasma gondiiis a zoonotic pathogen defined by three main clonal lineages (types I, II, III), of which type II is most common in Europe. Very few data exist on the prevalence and genotypes ofT. gondiiin the UK. Wildlife can act as sentinel species forT. gondiigenotypes present in the environment, which may subsequently be transmitted to livestock and humans. DNA was extracted from tissue samples of wild British carnivores, including 99 ferrets, 83 red foxes, 70 polecats, 65 mink, 64 badgers and 9 stoats. Parasite DNA was detected using a nested ITS1 PCR specific forT. gondii, PCR positive samples were subsequently genotyped using five PCR–RFLP markers.Toxoplasma gondiiDNA was detected within all these mammal species and prevalence varied from 6·0 to 44·4% depending on the host. PCR–RFLP genotyping identified type II as the predominant lineage, but type III and type I alleles were also identified. No atypical or mixed genotypes were identified within these animals. This study demonstrates the presence of alleles for all three clonal lineages with potential for transmission to cats and livestock. This is the first DNA-based study ofT. gondiiprevalence and genotypes across a broad range of wild British carnivores.

scholarly journals Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil

2020 ◽  
Vol 41 (1) ◽  
pp. 213
Author(s):  
Keila Jimenez Torrico ◽  
Nelson Jessé Rodrigues dos Santos ◽  
Hugo Luca Abate ◽  
Felippe Danyel Cardoso Martins ◽  
Luiz Daniel de Barros ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Giardia Duodenalis ◽  
Positive Sample ◽  
Pcr Analysis ◽  
Fecal Samples ◽  
Cryptosporidium Spp ◽  
Specific Pcr ◽  
Identity Match ◽  
Pcr Products ◽  
The City

Protozoans are widely distributed, and several species may parasitize the digestive tracts of cats (Felis catus), and can be transmitted to humans. The present study aimed to evaluate the species and occurrence of gastrointestinal protozoans in cats in the city of Londrina, Paraná, Brazil. A total of 206 cat fecal samples were tested, of which 141 were from shelter animals, and 65 were from pets owned by local people. Samples were processed by parasitological techniques. Coproparasitological techniques (Willis, Faust and Ziehl-Neelsen) were performed for detection of protozoan parasites. Subsequently, all samples were processed by PCR protocols specific to Toxoplasma gondii, Giardia spp., and Cryptosporidium spp. PCR products from positive samples were selected for sequencing. No samples were found to be positive for Cryptosporidium spp. using the Ziehl-Neelsen technique. Using specific PCR protocols, 1/206 (0.48%) samples tested positive for Cryptosporidium spp. After purification, this one positive sample was sequenced, and it demonstrated a 100% identity match to Cryptosporidium muris. Using specific PCR protocols, 13/206 (9.22%) cat fecal samples tested, including 2/65 (3.08%) pet cat fecal samples, were positive for T. gondii. PCR analysis revealed that 37/206 (17.96%) of cat fecal samples were positive for Giardia spp., including 27/141 (19.15%) of shelter cat fecal samples, and 10/65 (15.38%) pet cat fecal samples (p = 0.5124). When sequenced, these positive samples showed a 100% identity match with Giardia duodenalis. This study demonstrated that infections with Cryptosporidium spp., Toxoplasma gondii, and Giardia duodenalis are present in the population of both pet cats and shelter cats in the city of Londrina. This poses a risk to public health, because these parasites have a high zoonotic potential.

scholarly journals Isolation and genotyping of Toxoplasma gondii from pregnant dairy cows (Bos taurus) slaughtered

2012 ◽  
Vol 21 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
César Augusto Barbosa de Macedo ◽  
Maria Paula de Carvalho Ewald ◽  
Guilherme Felippelli Martins ◽  
Dauton Luiz Zulpo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dairy Cows ◽  
Blood Sample ◽  
Bos Taurus ◽  
Rflp Markers ◽  
Human Consumption ◽  
Mouse Bioassay ◽  
Type Ii ◽  
Tissue Samples ◽  
Pcr Rflp

The current study aimed to evaluate serology, and isolate and genotype Toxoplasma gondii strains from pregnant dairy cows, slaughtered in an abattoir for human consumption, and their fetuses. Blood from 60 pregnant dairy cows and blood and tissue samples (brain, lung, heart, and liver) from their fetuses were collected and analyzed in a mouse bioassay. Antibodies against T. gondii were observed in 48.3% of cows and 3.7% of fetuses (IFAT, titers ≥ 50 for cows and 25 for fetuses were considered positive). Fourteen fetuses (23.3%) and six cows (10.0%) were identified as positive in the bioassay. T. gondii was isolated from a blood sample of a cow older than 4 years old in the 6th month of pregnancy, and from a blood sample of a fetus in the 6th month of gestation. These isolates were identified by polymerase chain reaction (PCR) as being of T. gondii and both strains showed type II alleles for all PCR-restriction fragment length polymorphism (PCR-RFLP) markers tested. T. gondii type II strain from cattle was isolated for the first time in Brazil. The current study also showed that transplacental transmission of T. gondii naturally occurs in dairy cows (23.3%) from Southern Brazil.

Molecular and biological characterization ofToxoplasma gondiiisolates from free-range chickens from Guyana, South America, identified several unique and common parasite genotypes

Parasitology ◽  
2007 ◽  
Vol 134 (11) ◽  
pp. 1559-1565 ◽  
Author(s):  
J. P. DUBEY ◽  
L. APPLEWHAITE ◽  
N. SUNDAR ◽  
G. V. VELMURUGAN ◽  
L. A. BANDINI ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
South America ◽  
Rflp Markers ◽  
Free Range ◽  
Clonal Type ◽  
Modified Agglutination Test ◽  
Pcr Rflp ◽  
Free Ranging ◽  
Common Parasite

SUMMARYThe prevalence ofToxoplasma gondiiin free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence ofT. gondiioocysts in the soil because chickens feed from the ground. The prevalence ofT. gondiiin 76 free-range chickens from Guyana, South America was determined. Antibodies toT. gondiiwere assayed by the modified agglutination test (MAT), and found in 50 (65·8%) of 76 chickens with titres of 1:5 in four, 1:10 in one, 1:20 in five, 1:40 in seven, 1:80 in six, 1:160 in eight, 1:320 in four, 1:640 or higher in 15. Hearts and brains of 26 chickens with titres of <1:5 were pooled in 5 batches and bioassayed in mice. Hearts and brains of 50 chickens with titres of 1:5 or higher were bioassayed in mice.Toxoplasma gondiiwas isolated by bioassay in mice from 35 chickens with MAT titres of 1:20 or higher. All mice inoculated with tissues of 30 infected chickens remained asymptomatic.Toxoplasma gondiiisolates from 35 chickens were genotyped using 11 PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, a new SAG2, and Apico. A total of 9 genotypes were identified, with 5 genotypes (nos 1, 4, 5, 6 and 7) unique to Guyana, 2 genotypes (nos 2 and 3) previously identified in chickens from Brazil, 1 genotype (no. 8) previously identified in chickens from Brazil, Costa Rica and Nicaragua, and 1 genotype (no. 9) belonging to the clonal type III lineage that exists globally. Infection with 2 genotypes was found from 1 chicken. This is the first report of genetic characterization ofT. gondiiisolates from any host from Guyana.

Toxoplasma gondii: Genotyping of strains from Brazilian AIDS patients with cerebral toxoplasmosis by multilocus PCR–RFLP markers

2008 ◽  
Vol 118 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Isabelle M.R. Ferreira ◽  
José E. Vidal ◽  
Thais A. Costa-Silva ◽  
Cristina S. Meira ◽  
Roberto M. Hiramoto ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rflp Markers ◽  
Cerebral Toxoplasmosis ◽  
Aids Patients ◽  
Pcr Rflp

scholarly journals Prevalence of Toxoplasma gondii in pet and stray cats in Klang Valley, Malaysia

2020 ◽  
pp. 542-550
Author(s):  
Tan L.P. ◽  
Megat Abd. Rani P.A. ◽  
Sharma R.S.K. ◽  
Syed Hussain S.S. ◽  
Watanabe M.
Keyword(s):  
Toxoplasma Gondii ◽  
Health Hazard ◽  
Chi Square ◽  
Fecal Samples ◽  
Local Council ◽  
Worldwide Distribution ◽  
Stray Cats ◽  
Significant Difference ◽  
High Prevalence ◽  
Klang Valley

Toxoplasma gondii, a zoonotic protozoan that has a worldwide distribution, is known to infect many warm-blooded vertebrates. The feline species including domestic cats are the definitive hosts for Toxoplama gondii and shed the infective oocyst. There is lack of information on the prevalence of Toxoplasma gondii in cats in Malaysia. The objective of this study was to determine both the seroprevalence of T. gondii and the prevalence of T. gondii DNA in cats’ feces in Klang Valley, Malaysia. 198 blood and 201 fecal samples were collected from pet and stray cats from the local council, Dewan Bandaraya Kuala Lumpur (DBKL) and University Veterinary Hospital, Universiti Putra Malaysia respectively. The overall seroprevalence of Toxoplasma gondii in cats in the Klang Valley was found to be 5.5%. There was a high prevalence (10.5%) of T. gondii DNA detected in the cat fecal samples in both pet and stray cats suggestive of T. gondii oocyst shedding. Stray cats showed a higher seroprevalence and molecular prevalence of T. gondii than the pet cats. However, comparative analysis using Chi-square test showed no significant difference between both groups (P>0.05). Higher prevalence (10.5%) of cats shedding T. gondii DNA as compared to the seroprevalence (5.5%) was found in the cat population in the Klang Valley. The high prevalence of cats shedding T. gondii DNA is alarming as this may directly reflect the number of oocysts excreted into the environment posing a significant public health hazard.

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