Nerve growth factor is closely related to glucose metabolism, insulin sensitivity and insulin secretion in the second trimester: a case–control study in Chinese

Abstract Objective Inflammation-related factors have been shown to play a significant role throughout pregnancy. In this study, we aimed to explore the relationships between selected inflammatory cytokines and gestational diabetes (GDM) in Chinese pregnant women. Design and methods This was a 1:1 matched case–control study that included 200 pairs of subjects in the second trimester and 130 pairs of subjects in the third trimester. Serum levels of nerve growth factor (NGF), Interleukin-6 (IL-6), leptin, Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α) and Interleukin-1beta (IL-1β) were measured by enzyme immunoassay. The associations of these inflammatory factors with metabolic parameters were analysed. Results In the second trimester, GDM patients had higher NGF levels and lower IL-8 levels than did normal controls (P < 0.001 and P = 0.015, respectively). However, in the third trimester, only lower leptin levels were observed in the GDM group (P = 0.031). Additionally, in the second trimester, NGF levels were not only positively associated with fasting, 1-h and 2-h glucose levels and the area under curve of glucose, but also positively related to insulin sensitivity and secretion, as suggested by fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment index of β-cell secretion (HOMA-β) (all P < 0.05). Moreover, IL-6 and leptin levels were positively correlated with HOMA-IR and HOMA-β, and TNF-α levels were positively related to HOMA-IR (all P < 0.05). Except for the relationships between NGF and HOMA-β and TNF-α and HOMA-IR, the other correlations still existed even after adjusting for confounding factors (all P < 0.05). Conclusion In addition to the positive associations of IL-6 and leptin with insulin resistance and secretion, NGF was higher in the GDM patients and strongly linked to glucose metabolism, insulin resistance and pancreatic β cell function in Chinese pregnant women in the second trimester.

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Nerve Growth Factor is Closely Related to Glucose Metabolism, Insulin Sensitivity and Insulin Secretion in the Second Trimester: A Case-Control Study in Chinese

10.21203/rs.3.rs-40369/v1 ◽

2020 ◽

Author(s):

Mengyang Tang ◽

Mingjuan Luo ◽

Wenqian Lu ◽

Rong Zhang ◽

Wei Liang ◽

...

Keyword(s):

Insulin Resistance ◽

Case Control Study ◽

Model Assessment ◽

Second Trimester ◽

Homeostasis Model Assessment ◽

Third Trimester ◽

Β Cell ◽

The Third ◽

Tnf Α ◽

Control Study

Abstract Objective: Inflammation-related factors have been shown to play a significant role throughout pregnancy. In this study, we aimed to explore the relationships between selected inflammatory cytokines and gestational diabetes (GDM) in Chinese pregnant women.Design and Methods: This was a 1:1 matched case-control study that included 200 pairs of subjects in the second trimester and 130 pairs of subjects in the third trimester. Serum levels of nerve growth factor (NGF), Interleukin-6 (IL-6), leptin, Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α) and Interleukin-1beta (IL-1β) were measured by enzyme immunoassay. The associations of these inflammatory factors with metabolic parameters were analysed.Results: In the second trimester, GDM patients had higher NGF levels and lower IL-8 levels than did normal controls (P<0.001 and P=0.015, respectively). However, in the third trimester, only lower leptin levels were observed in the GDM group (P=0.031). Additionally, in the second trimester, NGF levels were not only positively associated with fasting, 1-h and 2-h glucose levels and the area under curve of glucose (AUCG), but also positively related to insulin sensitivity and secretion, as suggested by fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment index of β-cell secretion (HOMA-β) (all P<0.05). Moreover, IL-6 and leptin levels were positively correlated with HOMA-IR and HOMA-β, and TNF-α levels were positively related to HOMA-IR (all P<0.05). Except for the relationships between NGF and HOMA-β and TNF-α and HOMA-IR, the other correlations still existed even after adjusting for confounding factors (all P< 0.05). Conclusion: In addition to the positive associations of IL-6 and leptin with insulin resistance and secretion, NGF was higher in the GDM patients and strongly linked to glucose metabolism, insulin resistance and pancreatic β cell function in Chinese pregnant women in the second trimester.

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Nerve growth factor is closely related to glucose metabolism, insulin sensitivity and insulin secretion in the second trimester:a case-control study in Chinese

10.21203/rs.3.rs-40369/v2 ◽

2020 ◽

Author(s):

Mengyang Tang ◽

Mingjuan Luo ◽

Wenqian Lu ◽

Rong Zhang ◽

Wei Liang ◽

...

Keyword(s):

Insulin Resistance ◽

Case Control Study ◽

Model Assessment ◽

Second Trimester ◽

Homeostasis Model Assessment ◽

Third Trimester ◽

Β Cell ◽

The Third ◽

Tnf Α ◽

Control Study

Abstract Objective Inflammation-related factors have been shown to play a significant role throughout pregnancy. In this study, we aimed to explore the relationships between selected inflammatory cytokines and gestational diabetes (GDM) in Chinese pregnant women. Design and Methods This was a 1:1 matched case-control study that included 200 pairs of subjects in the second trimester and 130 pairs of subjects in the third trimester. Serum levels of nerve growth factor (NGF), Interleukin-6 (IL-6), leptin, Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α) and Interleukin-1beta (IL-1β) were measured by enzyme immunoassay. The associations of these inflammatory factors with metabolic parameters were analysed. Results In the second trimester, GDM patients had higher NGF levels and lower IL-8 levels than did normal controls ( P <0.001 and P =0.015, respectively). However, in the third trimester, only lower leptin levels were observed in the GDM group ( P =0.031). Additionally, in the second trimester, NGF levels were not only positively associated with fasting, 1-h and 2-h glucose levels and the area under curve of glucose (AUCG), but also positively related to insulin sensitivity and secretion, as suggested by fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment index of β-cell secretion (HOMA-β) (all P <0.05). Moreover, IL-6 and leptin levels were positively correlated with HOMA-IR and HOMA-β, and TNF-α levels were positively related to HOMA-IR (all P <0.05). Except for the relationships between NGF and HOMA-β and TNF-α and HOMA-IR, the other correlations still existed even after adjusting for confounding factors (all P < 0.05). Conclusion In addition to the positive associations of IL-6 and leptin with insulin resistance and secretion, NGF was higher in the GDM patients and strongly linked to glucose metabolism, insulin resistance and pancreatic β cell function in Chinese pregnant women in the second trimester.

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Nerve growth factor is closely related to glucose metabolism, insulin sensitivity and insulin secretion in the second trimester: a case-control study in Chinese

10.21203/rs.3.rs-40369/v3 ◽

2020 ◽

Author(s):

Mengyang Tang ◽

Mingjuan Luo ◽

Wenqian Lu ◽

Rong Zhang ◽

Wei Liang ◽

...

Keyword(s):

Insulin Resistance ◽

Case Control Study ◽

Model Assessment ◽

Second Trimester ◽

Homeostasis Model Assessment ◽

Third Trimester ◽

Β Cell ◽

The Third ◽

Tnf Α ◽

Control Study

Abstract Objective: Inflammation-related factors have been shown to play a significant role throughout pregnancy. In this study, we aimed to explore the relationships between selected inflammatory cytokines and gestational diabetes (GDM) in Chinese pregnant women.Design and Methods: This was a 1:1 matched case-control study that included 200 pairs of subjects in the second trimester and 130 pairs of subjects in the third trimester. Serum levels of nerve growth factor (NGF), Interleukin-6 (IL-6), leptin, Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α) and Interleukin-1beta (IL-1β) were measured by enzyme immunoassay. The associations of these inflammatory factors with metabolic parameters were analysed.Results: In the second trimester, GDM patients had higher NGF levels and lower IL-8 levels than did normal controls (P<0.001 and P=0.015, respectively). However, in the third trimester, only lower leptin levels were observed in the GDM group (P=0.031). Additionally, in the second trimester, NGF levels were not only positively associated with fasting, 1-h and 2-h glucose levels and the area under curve of glucose (AUCG), but also positively related to insulin sensitivity and secretion, as suggested by fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment index of β-cell secretion (HOMA-β) (all P<0.05). Moreover, IL-6 and leptin levels were positively correlated with HOMA-IR and HOMA-β, and TNF-α levels were positively related to HOMA-IR (all P<0.05). Except for the relationships between NGF and HOMA-β and TNF-α and HOMA-IR, the other correlations still existed even after adjusting for confounding factors (all P< 0.05).Conclusion: In addition to the positive associations of IL-6 and leptin with insulin resistance and secretion, NGF was higher in the GDM patients and strongly linked to glucose metabolism, insulin resistance and pancreatic β cell function in Chinese pregnant women in the second trimester.

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Study on the correlation between the changes of TNFR1, TNF-α, and adiponectin in patients with gestational diabetes mellitus and insulin resistance

European Journal of Inflammation ◽

10.1177/2058739219846346 ◽

2019 ◽

Vol 17 ◽

pp. 205873921984634

Author(s):

Jie Xie ◽

Lan Dai ◽

Xiaolei Tang

Keyword(s):

Insulin Resistance ◽

Research Group ◽

Cell Function ◽

Control Group ◽

Model Assessment ◽

Homeostasis Model Assessment ◽

Β Cell ◽

Tnf Α ◽

Β Cell Function ◽

Necrosis Factor

Gestational diabetes mellitus (GDM) refers to pregnant women with impaired glucose tolerance, which could bring high risk to the mother and fetus. However, the early diagnosis and treatment of GDM remained unclear. In this study, 60 patients with GDM were selected as the research group and 50 healthy pregnant women as the control group. Tumor necrosis factor receptor 1 (TNFR1), tumor necrosis factor-α (TNF-α), and adiponectin (ADP) in the serum were measured by enzyme-linked immunosorbent assays (ELISAs). The levels of fasting blood glucose (FBG), fasting insulin (FINS), and glycosylated hemoglobin (HbA1c) were also detected to calculate homeostasis model assessment insulin resistance index (HOMA-IR) and pancreatic β-cell function index (HOMA-HBCl). Compared with the control group, the serum levels of TNFR1, TNF-α, and HbA1c in research group were significantly increased ( P < 0.05), while ADP showed lower levels ( P < 0.01). Furthermore, FBG, FINS, and HOMA-IR were evidently increased ( P < 0.05), while homeostasis model assessment insulin secretion index (HOMA-β) and insulin sensitivity index (ISI) were decreased ( P < 0.05) in research group. TNFR and TNF-α were positively correlated with FBG, FINS, and HOMA-IR ( P < 0.05). In addition, there was a significant negative correlation between ADP and FINS and HOMA-IR ( P < 0.01). From logistic regression analysis, age, gestational age, FBG, FINS, TNFR1, TNF-α, and ADP ( P < 0.05) were shown to be risk factors to affect the function of islet β-cells. In conclusion, the high levels of TNFR1 and TNF-α and the low levels of ADP in the second trimester of pregnancy are the risk factors of GDM, which are related to the insulin resistance and impaired pancreatic β-cell function.

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Estimates of Insulin Secretory Function in Apparently Healthy Volunteers Vary as a Function of How the Relevant Variables Are Quantified

Clinical Chemistry ◽

10.1373/clinchem.2010.152744 ◽

2011 ◽

Vol 57 (4) ◽

pp. 627-632 ◽

Cited By ~ 5

Author(s):

Barry R Johns ◽

Fahim Abbasi ◽

Gerald M Reaven

Keyword(s):

Insulin Resistance ◽

Insulin Secretion ◽

Plasma Glucose ◽

Insulin Action ◽

Cell Function ◽

Model Assessment ◽

Pancreatic Β Cell ◽

Homeostasis Model Assessment ◽

Β Cell ◽

Β Cell Function

BACKGROUND Several surrogate estimates have been used to define relationships between insulin action and pancreatic β-cell function in healthy individuals. Because it is unclear how conclusions about insulin secretory function depend on specific estimates used, we evaluated the effect of different approaches to measurement of insulin action and secretion on observations of pancreatic β-cell function in individuals whose fasting plasma glucose (FPG) was <7.0 mmol/L (126 mg/dL). METHODS We determined 2 indices of insulin secretion [homeostasis model assessment of β-cell function (HOMA-β) and daylong insulin response to mixed meals], insulin action [homeostasis model assessment of insulin resistance (HOMA-IR) and steady-state plasma glucose (SSPG) concentration during the insulin suppression test], and degree of glycemia [fasting plasma glucose (FPG) and daylong glucose response to mixed meals] in 285 individuals with FPG <7.0 mmol/L. We compared the relationship between the 2 measures of insulin secretion as a function of the measures of insulin action and degree of glycemia. RESULTS Assessment of insulin secretion varied dramatically as a function of which of the 2 methods was used and which measure of insulin resistance or glycemia served as the independent variable. For example, the correlation between insulin secretion (HOMA-β) and insulin resistance varied from an r value of 0.74 (when HOMA-IR was used) to 0.22 (when SSPG concentration was used). CONCLUSIONS Conclusions about β-cell function in nondiabetic individuals depend on the measurements used to assess insulin action and insulin secretion. Viewing estimates of insulin secretion in relationship to measures of insulin resistance and/or degree of glycemia does not mean that an unequivocal measure of pancreatic β-cell function has been obtained.

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Perineal Distensibility Using Epi-no in Twin Pregnancies: Comparative Study with Singleton Pregnancies

ISRN Obstetrics and Gynecology ◽

10.1155/2014/124206 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Juliana Sayuri Kubotani ◽

Antonio Fernandes Moron ◽

Edward Araujo Júnior ◽

Miriam Raquel Diniz Zanetti ◽

Vanessa Cardoso Marques Soares ◽

...

Keyword(s):

Case Control Study ◽

Abdominal Circumference ◽

Third Trimester ◽

Anthropometric Data ◽

Cross Sectional ◽

The Third ◽

Nulliparous Women ◽

Twin Pregnancies ◽

Control Study ◽

Singleton Pregnancies

The aims of this study were to compare perineal distensibility between women with twin and singleton pregnancies and to correlate these women’s perineal distensibility with anthropometric data. This prospective cross-sectional case-control study was conducted among nulliparous women, of whom 20 were pregnant with twins and 23 with a single fetus. Perineal distensibility was evaluated in the third trimester by means of Epi-no, which was introduced into the vagina and inflated up to the maximum tolerable limit. It was then withdrawn while inflated and its circumference was measured. The unpaired Student’s t-test was used to compare perineal distensibility in the two groups and Pearson’s correlation coefficient (r) was used to correlate the pregnant women’s perineal distensibility with their anthropometric data. There was no difference in perineal distensibility between the twin group (16.51 ± 2.05 cm) and singleton group (16.13 ± 1.67 cm) (P=0.50). There was a positive correlation between perineal distensibility and abdominal circumference (r=0.36; P=0.01). The greater the abdominal circumference was, the greater the perineal distensibility was, regardless of whether the pregnancy was twin or singleton.

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Vaspin in newly and previously diagnosed Chinese type 2 diabetic females: a case-control study

Asian Biomedicine ◽

10.5372/1905-7415.0504.069 ◽

2011 ◽

Vol 5 (4) ◽

Cited By ~ 2

Author(s):

Ren-Nan Feng ◽

Cheng Wang ◽

Chang-Hao Sun ◽

Fu-Chuan Guo ◽

Chen Zhao ◽

...

Keyword(s):

Insulin Resistance ◽

Case Control Study ◽

Linear Regression Analysis ◽

Fasting Blood Glucose ◽

Case Control ◽

Model Assessment ◽

Healthy Controls ◽

Newly Diagnosed ◽

Control Study

AbstractBackground: Visceral adipose tissue-derived serine protease inhibitor (vaspin) is a novel adipocytokine. Several studies have indicated that vaspin may exert an important role in the development of metabolic disorders.Objective: Evaluate serum vaspin and its relation to clinical parameters in newly and previously diagnosed Chinese type 2 diabetes mellitus (T2DM) females as a case-control study.Materials and methods: One hundred twenty female participants (newly and previously diagnosed T2DM patients) were recruited from an affiliated hospital of Harbin Medical University. Sixty healthy female volunteers from various communities were included as controls. Anthropometric parameters, serum fasting blood glucose, fasting insulin, lipid profile, HbA1c, and vaspin were measured in each participant.Results: Serum vaspin levels were significantly lower in previously diagnosed T2DM patients (0.51±0.29 ng/mL) than in newly diagnosed T2DM patients (0.62±0.28 ng/mL) and healthy controls (0.69±0.31 ng/mL). However, there was no difference in serum vaspin between newly diagnosed T2DM patients and healthy controls. In multiple linear regression analysis, serum vaspin was significantly and positively associated with HbA1c in both newly and previously diagnosed T2DM patients, negatively associated with homeostasis model assessment of insulin resistance in previously diagnosed patients, and positively correlated with age and body mass index in healthy controls.Conclusion: Serum vaspin was significantly lower in previously diagnosed T2DM patients than in newly diagnosed T2DM patients and healthy controls. Serum vaspin might be a predictor of poor glucose control and insulin resistance in T2DM.

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Insulin Resistance and Beta Cell Dysfunction in Severe Falciparum Malaria with Multi Organ Dysfunction Syndrome (MODS)

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20aug309 ◽

2020 ◽

Vol 5 (8) ◽

pp. 1756-1759

Author(s):

Manoj Kumar Mohapatra ◽

Muralidhar Anantrao Sangle ◽

Prafulla Kumar Bariha

Keyword(s):

Insulin Resistance ◽

Falciparum Malaria ◽

Organ Dysfunction ◽

Cell Function ◽

Model Assessment ◽

Homeostasis Model Assessment ◽

Β Cell ◽

Cell Dysfunction ◽

Β Cell Function ◽

Severe Falciparum Malaria

Insulin Resistance is a major factor among patients with critical illness due to various causes. Severe falciparum malaria with MODS diagnosed as per the criteria of MSS and admitted to the Medical ward of our hospital were assessed for IR and β cell function by using homeostasis model assessment. 75 consecutive patients of SFM admitted to the Medical ward of our hospital were included in this study. Malaria was diagnosed as per criteria of WHO and organ dysfunction was diagnosed as per Malaria Severity Score. Insulin Resistance and β cell function was assessed by using homeostasis model assessment on Day-1 and Day-7. Out of 75 patients of severe falciparum malaria with MODS 2, 3, 4, and 5 organ dysfunctions constituted 16 (21.3%), 34 (45.3%), 16 (21.3%), and 9 (12.0%) patients, respectively.Hepatic failure was the most common organ system failure (n=58; 77.3%), followed by neurological (n=50;66.6%) ,renal (n=40;53.3%), hematological (n=30; 40.0%), and, respiratory failure ( n=15; 20.0%). Hyperglycemia was present in 25 (33.3%) cases where as normoglycemia was present in 50 (66.6%) cases. The values of FBS, Tg, insulin, IR, and β cell function decreased on Day-7 compared to Day-1 after recovery from critically ill state. The patients who died had a high insulin value, IR, but low β cell dysfunction compared to the survivors. This study showed that IR and β cell dysfunction were associated with severe malaria with MODS with increased mortality.

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Effects of dynamic change in fetuin-A levels from the first to the second trimester on insulin resistance and gestational diabetes mellitus: a nested case–control study

BMJ Open Diabetes Research & Care ◽

10.1136/bmjdrc-2019-000802 ◽

2020 ◽

Vol 8 (1) ◽

pp. e000802 ◽

Cited By ~ 1

Author(s):

Chuyao Jin ◽

Lizi Lin ◽

Na Han ◽

Zhiling Zhao ◽

Zheng Liu ◽

...

Keyword(s):

Insulin Resistance ◽

Case Control Study ◽

Cell Function ◽

Dynamic Change ◽

First Trimester ◽

Second Trimester ◽

Fetuin A ◽

Β Cell Function ◽

Nested Case Control ◽

Control Study

ObjectiveTo examine the effects of dynamic change in fetuin-A levels before the diagnosis of gestational diabetes mellitus (GDM) on insulin resistance and GDM.Research design and methodsA total of 135 women with GDM and 135 normal glucose tolerance (NGT) women with matched age (±2 years old) and gestational age at taking the oral glucose tolerance test (OGTT) were included in this nested case–control study. Fasting venous blood samples were collected at the prenatal visit of the first trimester and during OGTT of the second trimester. Plasma concentration of fetuin-A and insulin was determined.ResultsThe plasma fetuin-A concentration in women with GDM was significantly higher than NGT controls in both the first trimester (medians: 403.0 pg/mL vs 273.4 pg/mL; p<0.05) and the second trimester (medians: 475.7 pg/mL vs 290.8 pg/mL; p<0.05) and notably increased from the first to the second trimester. Multivariate linear regression analysis showed that the change in fetuin-A concentration was associated with the changes in fasting insulin, homeostasis model assessment (HOMA) of insulin resistance, and HOMA of β-cell function (HOMA-β) (p<0.05). The highest quartile of the increase in fetuin-A concentration from the first to the second trimester was associated with a higher risk of developing GDM compared with the lowest quartile (OR 2.14; 95% CI 1.05 to 4.37).ConclusionsThe dynamic change in fetuin-A levels was associated with the changes in insulin resistance and β-cell function from the first to the second trimester, and was associated with an increased risk of the development of GDM, indicating that fetuin-A could be a biomarker to predict the risk of GDM.Trial registration numberNCT03814395.

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