Transcriptomic analysis of male and female Schistosoma mekongi adult worms

Abstract Background: Melanoma has become the leading cause of death from skin disease, and male patients show higher mortality rate than female. Mitochondrial transplantation therapy is an active area of current research but the anti-melanoma activity and specific mechanism involved in the therapy remain to be fully characterized.Methods: In the study, we intravenously administrated mitochondria extracted from male and female mouse livers respectively to the mice bearing malignantly subcutaneous and metastatic melanoma, and identified the signal mechanism responsible for the mitochondrial treatment through transcriptomic analysis. Meanwhile, the efficiency of female mitochondria and male mitochondria was compared in the cultured melanoma cells and transplanted melanoma in mice. Results: The results suggested that the mitochondria significantly inhibited the tumor growth, and the transcriptomic analysis suggested that general chromosome silencing and local opening of UPRmt effect region were strongly associated with the mitochondria against melanoma., which represented cell cycle arrest, autophagy, and cell apoptosis in the mitochondrial therapy on the metastasis melanoma. Moreover, the anti-tumor activity of mitochondria from female animals was more efficient in comparison to the males, and the female mitochondria could probably induce more persuasive mitochondria-nuclear communication than the mitochondria from male mice.Conclusions: The study not only reveals the anti-tumor mechanism of the mitochondria but also provides a novel insight into the effect of mitochondria in different genders.

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Transcriptomic analysis of cardiac gene expression across the life course in male and female mice

Physiological Reports ◽

10.14814/phy2.14940 ◽

2021 ◽

Vol 9 (13) ◽

Author(s):

Aykhan Yusifov ◽

Vikram E. Chhatre ◽

Eva K. Koplin ◽

Cortney E. Wilson ◽

Emily E. Schmitt ◽

...

Keyword(s):

Gene Expression ◽

Life Course ◽

Transcriptomic Analysis ◽

Male And Female ◽

Female Mice ◽

Cardiac Gene Expression ◽

Cardiac Gene ◽

The Life Course

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Time course transcriptomic analysis of dihydrotestosterone (DHT) in male and female mouse adipose tissue

Signaling Pathways Project Datasets ◽

10.1621/9rrvtagzkb ◽

2015 ◽

Author(s):

Y Zhang

Keyword(s):

Adipose Tissue ◽

Female Mouse ◽

Time Course ◽

Transcriptomic Analysis ◽

Male And Female

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Transcriptomic Analysis Of Cardiac Gene Expression Across The Life‐Course In Male And Female Mice

The FASEB Journal ◽

10.1096/fasebj.2021.35.s1.02226 ◽

2021 ◽

Vol 35 (S1) ◽

Author(s):

Aykhan Yusifov ◽

Vikram Chhatre ◽

Eva Koplin ◽

Emily Schmitt ◽

Kathleen Woulfe ◽

...

Keyword(s):

Gene Expression ◽

Life Course ◽

Transcriptomic Analysis ◽

Male And Female ◽

Female Mice ◽

Cardiac Gene Expression ◽

Cardiac Gene ◽

The Life Course

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Phosphoproteomics analysis of male and female Schistosoma mekongi adult worms

Scientific Reports ◽

10.1038/s41598-019-46456-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Nattapon Simanon ◽

Poom Adisakwattana ◽

Tipparat Thiangtrongjit ◽

Yanin Limpanont ◽

Phiraphol Chusongsang ◽

...

Keyword(s):

Male And Female ◽

Schistosoma Mekongi

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The Clinical, Roentgenological and Morphological Effects of an Acute Exposure of Phosgene on the Lungs of Dogs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065237 ◽

1971 ◽

Vol 29 ◽

pp. 236-237

Author(s):

R. F. Bils ◽

W. F. Diller ◽

F. Huth

Keyword(s):

Latent Period ◽

Chemical Industry ◽

Toxic Substance ◽

Lung Edema ◽

X Rays ◽

Beagle Dogs ◽

Male And Female ◽

Morphological Alterations ◽

Before And After ◽

Electron Microscopes

Phosgene still plays an important role as a toxic substance in the chemical industry. Thiess (1968) recently reported observations on numerous cases of phosgene poisoning. A serious difficulty in the clinical handling of phosgene poisoning cases is a relatively long latent period, up to 12 hours, with no obvious signs of severity. At about 12 hours heavy lung edema appears suddenly, however changes can be seen in routine X-rays taken after only a few hours' exposure (Diller et al., 1969). This study was undertaken to correlate these early changes seen by the roengenologist with morphological alterations in the lungs seen in the'light and electron microscopes.Forty-two adult male and female Beagle dogs were selected for these exposure experiments. Treated animals were exposed to 94.5-107-5 ppm phosgene for 10 min. in a 15 m3 chamber. Roentgenograms were made of the thorax of each animal before and after exposure, up to 24 hrs.

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Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077086 ◽

1983 ◽

Vol 41 ◽

pp. 702-703

Author(s):

D. J. McComb ◽

J. Beri ◽

F. Zak ◽

K. Kovacs

Keyword(s):

Electron Microscopy ◽

Animal Model ◽

Epoxy Resin ◽

Immunoelectron Microscopy ◽

Tumor Type ◽

Gonadal Function ◽

Sprague Dawley ◽

Male And Female ◽

Reticulin Fibers ◽

Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

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Quantitative ultrastructural reconstruction of small regions within large volumes by correlative light and high voltage electron microscopy of serial 0.25-0.50 μm sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127396 ◽

1987 ◽

Vol 45 ◽

pp. 578-581

Author(s):

Conly L. Rieder ◽

Frederick J. Miller ◽

Edwin Davison ◽

Samuel S. Bowser ◽

Kirsten Lewis ◽

...

Keyword(s):

Electron Microscopy ◽

High Voltage ◽

Mitotic Spindle ◽

Meiotic Spindle ◽

High Voltage Electron Microscopy ◽

Male And Female ◽

Voltage Electron ◽

High Voltage Electron ◽

Differential Stability ◽

Sperm Aster

In this abstract we Illustrate how same-section correlative light and high voltage electron microscopy (HVEM) of serial 0.25-0.50-μm sections can answer questions which are difficult to approach by EM of 60-100 nm sections.Starfish (Pisaster and Asterlas) eggs are fertilized at meiosis I when the oocyte contains two maternal centrosomes (e.g., asters) which form the poles of the first meiotic spindle. Immediately after fertilization a sperm aster is assembled in the vicinity of the male pronucleus and persists throughout meiosis. At syngamy the sperm aster splits to form the poles of the first mitotic spindle. During this time the functional and replicative properties of the maternal centrosome, inherited from the last meiotic division, are lost. The basis for this differential stability, of male and female centrosomes in the same cytoplasm, is a mystery.

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