scholarly journals Systematic review on use, cost and clinical efficacy of automated decontamination devices

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Stephanie J. Dancer ◽  
Marco-Felipe King
Keyword(s):  
Systematic Review ◽  
Hydrogen Peroxide ◽  
Infection Control ◽  
Uv Light ◽  
Objective Assessment ◽  
Control Group ◽  
Environmental Damage ◽  
Industry Sponsorship ◽  
Surface Decontamination

Abstract Background More evidence is emerging on the role of surface decontamination for reducing hospital-acquired infection (HAI). Timely and adequate removal of environmental pathogens leads to measurable clinical benefit in both routine and outbreak situations. Objectives This systematic review aimed to evaluate published studies describing the effect of automated technologies delivering hydrogen peroxide (H202) or ultra-violet (UV) light on HAI rates. Methods A systematic review was performed using relevant search terms. Databases were scanned from January 2005 to March 2020 for studies reporting clinical outcome after use of automated devices on healthcare surfaces. Information collected included device type, overall findings; hospital and ward data; study location, length and size; antimicrobial consumption; domestic monitoring; and infection control interventions. Study sponsorship and duplicate publications were also noted. Results While there are clear benefits from non-touch devices in vitro, we found insufficient objective assessment of patient outcome due to the before-and-after nature of 36 of 43 (84%) studies. Of 43 studies, 20 (47%) used hydrogen peroxide (14 for outbreaks) and 23 (53%) used UV technology (none for outbreaks). The most popular pathogen targeted, either alone or in combination with others, was Clostridium difficile (27 of 43 studies: 63%), followed by methicillin-resistant Staphylococcus aureus (MRSA) (16 of 43: 37%). Many owed funding and/or personnel to industry sponsorship (28 of 43: 65%) and most were confounded by concurrent infection control, antimicrobial stewardship and/or cleaning audit initiatives. Few contained data on device costs and rarely on comparable costs (1 of 43: 2%). There were expected relationships between the country hosting the study and location of device companies. None mentioned the potential for environmental damage, including effects on microbial survivors. Conclusion There were mixed results for patient benefit from this review of automated devices using H202 or UV for surface decontamination. Most non-outbreak studies lacked an appropriate control group and were potentially compromised by industry sponsorship. Concern over HAI encourages delivery of powerful disinfectants for eliminating pathogens without appreciating toxicity or cost benefit. Routine use of these devices requires justification from standardized and controlled studies to understand how best to manage contaminated healthcare environments.

scholarly journals Impact of Probiotic on the Number of Lactic Acid Rods Forming Hydrogen Peroxide Isolated From Porkers and on Changes in Drug Resistance of Selected Escherichia Coli Isolates

2012 ◽  
Vol 56 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Marek Selwet ◽  
Mariola Galbas ◽  
Piotr Dullin
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Drug Resistance ◽  
Lactic Acid ◽  
Control Group ◽  
Large White ◽  
E Coli ◽  
Probiotic Preparation ◽  
Hydrogen Oxide

Abstract The presented investigations were conducted on a group of 60 porkers of crossbreed Polish Landrace x Large White Polish. The animals were divided into two equal experimental groups. The control group (K) was fed diets without supplementation with probiotics, group (P) - diets with the addition of probiotic (0.2 kg t-1 feed). The aim of the study was to determine the effect of probiotic preparation on total numberof lactic acid rods from the Lactobacillus genus and those forming hydrogen oxide. The second part of experiment concerned the influence of probiotic preparation on the number, haemolytic ability and changes in drug resistance of Escherichia coli isolated from animal faeces. The significantly highest number of Lactobacillus sp. were determined in the saliva of porkers fed diets with the addition of probiotic, while the lowest in the control group. Lactobacillus sp. rods capable of forming hydrogen peroxide were isolated from 17 animals in group K and from three animals in group P. E. coli was determined in each examined sample of faeces. In groups K and P, counts of these bacteria were similar and did not differ statistically. High numbers of haemolytic isolates (haemolysis β) were found in faeces of animals fed diets with the addition of probiotic. Number and proportions of resistant isolates in groups K and P were different. Gentamicin was characterised by exceptionally high in vitro effectiveness. The used probiotic increased drug resistance of E. coli and increased frequency of incidence of haemolysis β.

scholarly journals Can Whitening Strips interfere with the Bond Strength of Composite Resins?

2015 ◽  
Vol 16 (4) ◽  
pp. 259-263
Author(s):  
Mateus Rodrigues Tonetto ◽  
Rudys Rodolfo de Jesus Tavarez ◽  
Leily Macedo Firoozmand ◽  
Matheus Coelho Bandeca ◽  
Shilpa H Bhandi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Bond Strength ◽  
Artificial Saliva ◽  
Large Fraction ◽  
Testing Machine ◽  
Potassium Nitrate ◽  
Composite Resins ◽  
Control Group ◽  
Bond Interface

ABSTRACT Aim The aim of this study was to investigate in vitro the bond strength of composite resins on enamel previously treated with whitening strips. Materials and methods A total of 48 bovine incisors were allocated to four experimental groups (n = 12 each): G1 (WSC)— treated with 9.5% hydrogen peroxide whitening strips (3D White Whitestrips® Advanced Vivid/CREST); G2 (WSO)—treated with 10% hydrogen peroxide whitening strips (3D WhiteTM/Oral B); G3 (WG)—treated with 7.5% hydrogen peroxide gel with fluorine, calcium and potassium nitrate (White Class®/FGM); and G4 (C)—control not subjected to bleaching treatment. The specimens were subjected to bleaching over 2 weeks following the manufacturers’ instructions. Following the elaboration of the composite resin test specimens, the samples were stored in artificial saliva and subsequently subjected to the microshear test using the universal testing machine (EMIC®). The bond strength values were analyzed by one-way ANOVA and Tukey's statistical test (5%). Results Significant differences were observed among the investigated groups (p < 0.05). The G3-WG exhibited greater values compared with the control group and the groups treated with strips, G1-WSC and G2-WSO. Analysis of the bond interface revealed that a large fraction of the failures occurred at the enamel-resin interface. Conclusion The bond strength decreased following 14 days of treatment with bleaching strips, whereas the whitening gel with 7.5% hydrogen peroxide, calcium and fluorine increased the bond strength. How to cite this article Firoozmand LM, dos Reis WLM, Vieira MA, Nunes AG, de Jesus Tavarez RR, Tonetto MR, Bramante FS, Bhandi SH, de Oliveira Roma RV, Bandeca MC. Can Whitening Strips interfere with the Bond Strength of Composite Resins? J Contemp Dent Pract 2015;16(4):259-263.

scholarly journals Influence of Simulated Pulpal Pressure on Efficacy of Bleaching Gels

2014 ◽  
Vol 15 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Carlos Rocha Gomes Torres ◽  
Graziela Ribeiro Batista ◽  
Alessandra Bühler Borges ◽  
Paula Tamião Arantes ◽  
Annette Wiegand ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Artificial Saliva ◽  
Negative Control ◽  
Color Variation ◽  
Color Measurement ◽  
Control Group ◽  
Significant Difference ◽  
Bleaching Gels ◽  
Bleaching Treatment

ABSTRACT Aim The aim of this study was to investigate the influence of simulated pulpal pressure on efficacy of bleaching gels. Materials and methods Cylindrical enamel-dentin specimens from bovine teeth (3 mm diameter, enamel and dentin layer each 1 mm thick) were divided into 4 groups, according to the bleaching treatment: negative control (non-bleached), bleached with 10% carbamide peroxide (CP), bleached with 7.5% hydrogen peroxide (HP) and bleached with 35% hydrogen peroxide. Ten percent CP gel was applied for 8 h/day and 7.5% HP for 1 h/day, during 14 days. For 35% HP treatment, two sessions of 45 minutes each were employed. In intermediate periods specimens were stored in artificial saliva. Experimental groups (n = 19) were subdivided according to the simulation of pulpal pressure (25 mm Hg) during bleaching treatment. Initial color measurement and after bleaching treatment were assessed by spectrophotometry, using CIE L*a*b* system. The data were statistically analyzed by ANOVA and Dunnett's posthoc tests (p < 0.05). Results There was significant difference of ∆E for all experimental groups compared to negative control group, according to Dunnett's test (p < 0.0001). There were no significant difference for total color variation (∆E) among experimental groups (p > 0.05). Conclusion It was concluded that all bleaching gels showed bleaching efficacy compared to non-bleached group and that the simulated pulpal pressure did not influence the bleaching outcomes of the tested gels. Clinical significance Although numerous in vitro studies investigating the efficacy of bleaching agents have been performed, they do not properly simulate the pulpal pressure. In order to make these studies closer to clinical conditions, it is important to reproduce these conditions in laboratory, so the results can be more reliable. This in vitro study was performed under simulated pulpal pressure, aiming to investigate its influence on dental bleaching outcomes. How to cite this article Borges AB, Batista GR, Arantes PT, Wiegand A, Attin T, Torres CRG. Influence of Simulated Pulpal Pressure on Efficacy of Bleaching Gels. J Contemp Dent Pract 2014;15(4):407-412.

54 CHEMICAL ENUCLEATION OF IN VITRO-MATURED BUFFALO (BUBALUS BUBALIS) OOCYTES INDUCED BY DEMECOLCINE FOR HANDMADE SOMATIC CELL CLONING

2008 ◽  
Vol 20 (1) ◽  
pp. 108
Author(s):  
R. A. Shah ◽  
M. S. Chauhan ◽  
R. S. Manik ◽  
S. K. Singla
Keyword(s):  
Somatic Cell ◽  
Nuclear Transfer ◽  
Uv Light ◽  
Genetic Material ◽  
Bubalus Bubalis ◽  
Significant Loss ◽  
Control Group ◽  
Cone Formation ◽  
Handmade Cloning

Cloning by somatic cell nuclear transfer requires enucleation of the recipient oocyte to remove its genetic material. In handmade cloning, a simplified cloning procedure (Vajta et al. 2001 Cloning 3, 89–95), zona-free oocytes are manually bisected, stained, and exposed to UV light for selection of demi-oocytes devoid of chromosomes. Development of procedures for enucleation which avoid exposure to UV light and conserve most of the cytoplasmic volume are necessary for improving the efficiency of handmade cloning. Chemically assisted enucleation protocols involve treating cumulus–oocyte complexes (COCs) during IVM with cytoskeleton-modifying agents, like demecolcine, which induce protrusion cone formation on the surface of the oocytes (Tani et al. 2006 Cloning Stem Cells 8, 61–66). Such a cone-like structure can then be easily excised with a microblade and enucleation of the oocyte achieved without a significant loss of cytoplasm. The aim of the present study was to establish an efficient protocol for demecolcine treatment of buffalo COCs during IVM to obtain the maximal proportion of oocytes where chromosomes are either expelled into the surface protrusion cone or completely enucleated. In Experiment I, COCs (n = 244), obtained from slaughterhouse buffalo ovaries and matured in vitro in TCM-199 (containing 10% FBS, 5 µg mL–1 pFSH, and 0.81 mm sodium pyruvate) at 38.5�C (in 5% CO2, 90–95% relative humidity), were treated in two groups with demecolcine (0.5 µg mL–1) beginning at either 15 h or 19 h from the start of IVM up to the end of IVM at 22 h, and compared to a control group (without demecolcine). Data were analyzed using ANOVA. The proportion of oocytes where a protrusion cone was observed was greatest (P < 0.05) in the 15-h treatment group (84%, n = 72/86) compared to those in either the 19-h or control groups (58%, n = 46/78, and 60%, n = 48/80, respectively). The presence of demecolcine for the last 7 h of IVM appears to have a significant effect on protrusion cone formation in buffalo oocytes. In Experiment II, COCs (n = 276)were divided into four groups, matured in vitro this time for 24 h and treated with demecolcine (0.5 µg mL–1) from the onset of IVM and up to 18, 21, or 24 h, and compared to a control group without demecolcine. Protrusion cone formation was observed in 72% (52/72), 66% (40/60), 62% (40/64), and 70% (56/80) of oocytes, respectively, in these groups. These percentages did not differ significantly (P < 0.05). However, the 21-h treatment resulted in complete enucleation in 32% (20/64) of oocytes, which was significantly greater (P < 0.05) than that in the other three groups where no such enucleation was observed. It can be hypothesized that demecolcine-free treatment from 21 to 24 h may have assisted in inducing complete enucleation of a significant number of treated oocytes. In conclusion, these results show that demecolcine-assisted and induced enucleation procedures can be used for increasing the efficiency of oocyte enucleation in handmade cloning and other nuclear transfer procedures in buffalo.

Airborne hydrogen peroxide for disinfection of the hospital environment and infection control: a systematic review

2011 ◽  
Vol 78 (3) ◽  
pp. 171-177 ◽  
Author(s):  
M.E. Falagas ◽  
P.C. Thomaidis ◽  
I.K. Kotsantis ◽  
K. Sgouros ◽  
G. Samonis ◽  
...  
Keyword(s):  
Systematic Review ◽  
Hydrogen Peroxide ◽  
Infection Control ◽  
Hospital Environment

scholarly journals Influence of Hydrogen Peroxide on Mineralization in Dental Pulp Cells: A Systematic Review

2021 ◽  
Vol 2 ◽  
Author(s):  
Alexandre Henrique dos Reis-Prado ◽  
Isadora Rodrigues Grossi ◽  
Hebertt Gonzaga dos Santos Chaves ◽  
Carolina Bosso André ◽  
Luís Fernando dos Santos Alves Morgan ◽  
...  
Keyword(s):  
Systematic Review ◽  
Hydrogen Peroxide ◽  
Dental Pulp ◽  
Cochrane Library ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Alp Activity ◽  
Dental Hard Tissues ◽  
Pulp Cells

Background: Dental bleaching agents show the ability to permeate through dental hard tissues, which may lead to pulp tissue changes. This systematic review (PROSPERO register: CRD42020213767) is aimed at understanding the effects of bleaching agents on the process of mineralization of the pulp tissue.Methods: Only in vitro studies evaluating the influence of hydrogen peroxide (HP) on mineralization in dental pulp cells were included. Studies without a non-bleached control group or cells after co-treatment with a bleaching agent other than HP and/or carbamide peroxide were excluded. The primary outcomes evaluated were alkaline phosphatase (ALP) activity and mineralized nodule deposition. The mineralization markers analysis in dental pulp cells and the cell viability were considered secondary outcomes. Two independent authors conducted a systematic search (PubMed/MEDLINE, Scopus, Embase, Cochrane Library, and OpenGrey until January 2021) with no language restrictions and performed data extraction. The quality assessment was appraised according to a modified Joanna Briggs Institute critical appraisal checklist.Results: The search resulted in 473 studies, and 11 were considered eligible. Overall, a reduction in the process of mineralization was observed among pulp cells after bleaching. A reduction in the ALP activity was reported in the mostly bleached groups using different protocols and analysis periods of nine studies. Regarding mineralized nodule deposition, 6 studies reported a significant reduction from 7 to 21 days among bleached groups. Of those three studies that investigated other mineralization markers, two found a reduction in the expression of dentin matrix acidic phosphoprotein (DMP)-1, dentin sialophosphoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) among some bleaching gel concentrations. In contrast, one study showed a greater expression of osteopontin (OPN) and osteocalcin (OCN) in 100 μmol/L HP after 5 or 10 min of exposure, and another study showed significant induction of DSPP in concentrations of up to 0.5 mmol/L HP.Conclusion: Especially, high concentrations of bleaching gel reduce the potential of mineralization in pulp cells in in vitro studies; however, different HP concentrations, bleaching protocols, and analysis periods can influence this outcome.

scholarly journals Evaluation of surface roughness and color change of bovine enamel after immersion in dye solution

2018 ◽  
Vol 5 (5) ◽  
pp. 95
Author(s):  
Ana Carolina Trentino ◽  
Larissa Marinho Azevedo ◽  
Felipe Fabrício Farias Da Silva ◽  
Maria Cristina Carvalho de Almedra Freitas ◽  
Marina Studart Alencar Borges ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Surface Roughness ◽  
Color Change ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Black Tea ◽  
Control Group ◽  
Change Analysis ◽  
Miller Test

Aim: The aim of this in vitro study was to evaluate the influence of dye solution on enamel color change after bleaching protocols and the effectiveness in maintaining the color of these agents.Material and Methods: The buccal surfaces of sixty-five bovine incisors were cleaned and polished, and the enamel specimens were divided into thirteen groups: G1 to G6: treated with 6% hydrogen peroxide using different surface agents; G7 to G12: treated with 15% hydrogen peroxide using different surface agents; G13: control. After 24 hours, the groups treated were immersed in black tea solution; the control group was stored in artificial saliva. The color was evaluated prior to bleaching, 24 hours later and after immersion in the dye solution; the roughness was measured immediately after bleaching, 24 hours later and 7 days after immersion in the dye solution. The data was analyzed using the Kruskal-Wallis test, followed by the Miller test for roughness analysis, and the Duncan test for color change analysis. It was used 5% significant level with p<0.05.Results: The data found in the evaluation of surface roughness after bleaching indicated a reduction of roughness in all the groups. The surface agent Bifluoride, when applied, showed an increase in roughness after its application and it decreases after immersion in dye solution; the surface agent Desensibilize and the XP Bond adhesive showed greater color alteration after immersion in dye solution.Conclusions: All the groups studied, under different whitening technique, were effective in promoting whitening.

Protective Effect of Saikosaponin B2 on Damage of Cultured SH-SY5Y Cells in Vitro Introduced by Hydrogen Peroxide

2017 ◽  
Vol 41 (S1) ◽  
pp. S707-S707 ◽  
Author(s):  
Y. Zhang ◽  
F. Liu ◽  
Z. Dai ◽  
Q. Wu
Keyword(s):  
Hydrogen Peroxide ◽  
Protective Effect ◽  
Volume Fraction ◽  
Calf Serum ◽  
Treated Group ◽  
Control Group ◽  
Morphological Identification ◽  
Model Group ◽  
Competing Interest

ObjectiveTo investigate the effect of saikosaponin B2 on the damage of cultured SH-SY5Y cells.Methods10% calf serum including volume fraction 0.05, 0.10, 0.20 saikosaponin B2 (10−4mol·L−1) were added respectively into the SH-SY5Y cells, which were then treated with 140 μmol· L−1 hydrogen peroxide(H2O2). 10% calf serum group and blank serum without H2O2-treated group were as the model group and the control group. The effect of saikosaponin B2 was observed by morphological identification, colorimetric MTT assay.ResultsBoth saikosaponin B2 of 10−6mol·L−1 and 2 × 10−6mol·L−1 can relieve the damage of SH-SY5Y cells and increase the survival of the cells.Conclusionsaikosaponin B2 can protect the cultured SH-SY5Y cells from damage induced by H2O2.Disclosure of interestThe authors have not supplied their declaration of competing interest.

scholarly journals Methods to disinfect and decontaminate SARS-CoV-2: a systematic review of in vitro studies

2021 ◽  
Vol 8 ◽  
pp. 204993612199854
Author(s):  
Chun Shing Kwok ◽  
Mustafa Dashti ◽  
Jacopo Tafuro ◽  
Mojtaba Nasiri ◽  
Elena-Andra Muntean ◽  
...  
Keyword(s):  
Systematic Review ◽  
Povidone Iodine ◽  
Uv Light ◽  
Light Irradiation ◽  
Uv Light Irradiation ◽  
Iodine Complex ◽  
Chemical Agents ◽  
Hand Wash ◽  
Different Types

Background: Cleaning is a major control component for outbreaks of infection. However, for the SARS-CoV-2 pandemic, there is limited specific guidance regarding the proper disinfection methods that should be used. Methods: We conducted a systematic review of the literature on cleaning, disinfection or decontamination methods in the prevention of SARS-CoV-2. Results: A total of 27 studies were included, reporting a variety of methods with which the effectiveness of interventions were assessed. Virus was inoculated onto different types of material including masks, nasopharyngeal swabs, serum, laboratory plates and simulated saliva, tears or nasal fluid and then interventions were applied in an attempt to eliminate the virus including chemical, ultraviolet (UV) light irradiation, and heat and humidity. At body temperature (37°C) there is evidence that the virus will not be detectable after 2 days but this can be reduced to non-detection at 30 min at 56°C, 15 min at 65°C and 2 min at 98°C. Different experimental methods testing UV light have shown that it can inactivate the virus. Light of 254–365 nm has been used, including simulated sunlight. Many chemical agents including bleach, hand sanitiser, hand wash, soap, ethanol, isopropanol, guandinium thiocynate/t-octylphenoxypolyethoxyethanol, formaldehyde, povidone-iodine, 0.05% chlorhexidine, 0.1% benzalkonium chloride, acidic electrolysed water, Clyraguard copper iodine complex and hydrogen peroxide vapour have been shown to disinfect SARS-CoV-2. Conclusions: Heating, UV light irradiation and chemicals can be used to inactivate SARS-CoV-2 but there is insufficient evidence to support one measure over others in clinical practice.

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