scholarly journals Production of spiculisporic acid by Talaromyces trachyspermus in fed-batch bioreactor culture

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Maki Moriwaki-Takano ◽  
Chikako Asada ◽  
Yoshitosi Nakamura
Keyword(s):  
High Surface ◽  
Total Yield ◽  
Nitrogen Sources ◽  
Crystalline Substance ◽  
Bioreactor Culture ◽  
Fed Batch ◽  
Meat Extract ◽  
Trace Metal Ions ◽  
Medium Components ◽  
Batch Bioreactor

AbstractSpiculisporic acid (SA) is a fatty acid-type biosurfactant with one lactone ring and two carboxyl groups. It has been used in metal removers and cosmetics, because of its low propensity to cause irritation to the skin, its anti-bacterial properties, and high surface activity. In the present study, we report an effective method for producing SA by selecting a high-producing strain and investigating the effective medium components, conditions, and environments for its culture. Among the 11 kinds of Talaromyces species, T. trachyspermus NBRC 32238 showed the highest production of a crystalline substance, which was determined to be SA using NMR. The strain was able to produce SA under acidic conditions from hexoses, pentoses, and disaccharides, with glucose and sucrose serving as the most appropriate substrates. Investigation of nitrogen sources and trace metal ions revealed meat extract and FeCl3 as components that promoted SA production. Upon comparing the two types of cultures with glucose in a baffle flask or aeration bioreactor, SA production was found to be slightly higher in the flask than in the reactor. In the bioreactor culture, sucrose was found to be an appropriate substrate for SA production, as compared to glucose, because with sucrose, the lag time until the start of SA production was shortened. Finally, fed-batch culture with sucrose resulted in 60 g/L of SA, with a total yield of 0.22 g SA/g sucrose and a productivity of 6.6 g/L/day.

scholarly journals Production of spiculisporic acid by Talaromyces trachyspermus in fed-batch bioreactor culture

2021 ◽  
Author(s):  
Maki Moriwaki-Takano ◽  
Chikako Asada ◽  
Yoshitosi Nakamura
Keyword(s):  
High Surface ◽  
Total Yield ◽  
Antibacterial Properties ◽  
Nitrogen Sources ◽  
Crystalline Substance ◽  
Bioreactor Culture ◽  
Fed Batch ◽  
Meat Extract ◽  
Trace Metal Ions ◽  
Batch Bioreactor

Abstract Spiculisporic acid (SA) is a fatty acid-type biosurfactant with one lactone ring and two carboxyl groups. It has been used in metal removers and cosmetics, because of its low propensity to cause irritation to the skin, its antibacterial properties, and high surface activity. In the present study, we report an effective method for producing SA by selecting a high-producing strain and investigating the effective medium components, conditions, and environments for its culture. Among the 11 kinds of Talaromyces species, T. trachyspermus NBRC 32238 showed the highest production of a crystalline substance, which was determined to be SA using NMR. The strain was able to produce SA under acidic conditions from hexoses, pentoses, and disaccharides, with glucose and sucrose serving as the most appropriate substrates. Investigation of nitrogen sources and trace metal ions revealed meat extract and FeCl3 as components that promoted SA production. Upon comparing the two types of cultures with glucose in a baffle flask or aeration bioreactor, SA production was found to be slightly higher in the flask than in the reactor. In the bioreactor culture, sucrose was found to be an appropriate substrate for SA production, as compared to glucose, because with sucrose, the lag time until the start of SA production was shortened. Finally, fed-batch culture with sucrose resulted in 60 g/L of SA, with a total yield of 0.22 g SA/g sucrose and a productivity of 6.6 g/L/d.

Evaluation of nitrogenous media components by Plackett–Burman statistical design for β-d-fructofuranosidase production by Saccharomyces sp. strain GVT263

2009 ◽  
Vol 55 (4) ◽  
pp. 405-409 ◽  
Author(s):  
M. Venkateshwar ◽  
K. Chaitanya ◽  
M. D. Altaf ◽  
B. Hameeda ◽  
M. Ghopal Reddy
Keyword(s):  
Experimental Design ◽  
Yeast Extract ◽  
Ammonium Acetate ◽  
Statistical Design ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Statistical Experimental Design ◽  
Meat Extract ◽  
Medium Components ◽  
Sucrose Fermentation

β-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using a high enzyme-producing yeast strain. Plackett–Burman statistical experimental design was applied to evaluate the fermentation medium components. The effects of 10 nitrogen sources were studied in a 16-run experimental design. Beef extract, yeast extract, N-Z-amine, tryptone, meat extract, and ammonium acetate were found to have significant effects on enzyme production. Among these, yeast extract, N-Z-amine, and ammonium acetate were the most significant. A maximum FFase activity of 299.4 U/mL was obtained after a 24 h fermentation period.

Simulation of UDMC on a Fed-Batch Bioreactor

1988 ◽  
Author(s):  
Hsin-Ying Lin ◽  
John C. Lewis ◽  
Richard H. Luecke
Keyword(s):  
Fed Batch ◽  
Batch Bioreactor

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

scholarly journals Bioreactor Parameters for Microcarrier-Based Human MSC Expansion under Xeno-Free Conditions in a Vertical-Wheel System

2020 ◽  
Vol 7 (3) ◽  
pp. 73 ◽  
Author(s):  
Josephine Lembong ◽  
Robert Kirian ◽  
Joseph D. Takacs ◽  
Timothy R. Olsen ◽  
Lye Theng Lock ◽  
...  
Keyword(s):  
Cost Effective ◽  
Population Doubling ◽  
Bioreactor Culture ◽  
Lot Size ◽  
Fed Batch ◽  
The Media ◽  
Manufacturing Technologies ◽  
Suspension Bioreactor ◽  
Culture Protocol ◽  
Bioreactor Process

Human mesenchymal stem/stromal cells (hMSCs) have been investigated and proven to be a well-tolerated, safe therapy for a variety of indications, as shown by over 900 registered hMSC-based clinical trials. To meet the commercial demand for clinical manufacturing of hMSCs, production requires a scale that can achieve a lot size of ~100B cells, which requires innovative manufacturing technologies such as 3D bioreactors. A robust suspension bioreactor process that can be scaled-up to the relevant scale is therefore crucial. In this study, we developed a fed-batch, microcarrier-based bioreactor process, which enhances media productivity and drives a cost-effective and less labor-intensive hMSC expansion process. We determined parameter settings for various stages of the culture: inoculation, bioreactor culture, and harvest. Addition of a bioreactor feed, using a fed-batch approach, was necessary to replenish the mitogenic factors that were depleted from the media within the first 3 days of culture. Our study resulted in an optimized hMSC culture protocol that consistently achieved hMSC densities between 2 × 105–6 × 105 cells/mL within 5 days with no media exchange, maintaining the final cell population doubling level (PDL) at 16–20. Using multiple hMSC donors, we showed that this process was robust and yielded hMSCs that maintained expansion, phenotypic characteristic, and functional properties. The developed process in a vertical-wheel suspension bioreactor can be scaled to the levels needed to meet commercial demand of hMSCs.

scholarly journals Neural Network-Based State Estimation for a Closed-Loop Control Strategy Applied to a Fed-Batch Bioreactor

Complexity ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Santiago Rómoli ◽  
Mario Serrano ◽  
Francisco Rossomando ◽  
Jorge Vega ◽  
Oscar Ortiz ◽  
...  
Keyword(s):  
Neural Network ◽  
Closed Loop ◽  
Rbf Neural Network ◽  
Control Strategies ◽  
Tracking Error ◽  
Closed Loop Control ◽  
Online Information ◽  
Fed Batch ◽  
Loop Control ◽  
Batch Bioreactor

The lack of online information on some bioprocess variables and the presence of model and parametric uncertainties pose significant challenges to the design of efficient closed-loop control strategies. To address this issue, this work proposes an online state estimator based on a Radial Basis Function (RBF) neural network that operates in closed loop together with a control law derived on a linear algebra-based design strategy. The proposed methodology is applied to a class of nonlinear systems with three types of uncertainties: (i) time-varying parameters, (ii) uncertain nonlinearities, and (iii) unmodeled dynamics. To reduce the effect of uncertainties on the bioreactor, some integrators of the tracking error are introduced, which in turn allow the derivation of the proper control actions. This new control scheme guarantees that all signals are uniformly and ultimately bounded, and the tracking error converges to small values. The effectiveness of the proposed approach is illustrated on the basis of simulated experiments on a fed-batch bioreactor, and its performance is compared with two controllers available in the literature.

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