Comparative study of bone regeneration using fibrin sealant with xenograft in rabbit sinus: pilot study

Abstract Background Stability of the grafted bone volume is one of the important factors to the success of alveolar bone grafts. For this, platelet-rich plasma (PRP) or fibrin sealant is mixed with the bone graft material. Bio-Oss® is a protein-free bovine mineral commonly used in bone graft procedures. The grafting particles are commonly combined with a standard fibrin sealant (Tisseel®) to fabricate a plastic implantable product. The purpose of this experiment was to evaluate the efficacy of fibrin sealant (Tisseel®) in bone regeneration performance in a rabbit maxillary sinus model. Methods A total of five 3.5 kg weight New Zealand white rabbits were used for the study. After elevating the sinus membrane in both maxillary sinus cavities, Bio-Oss® mixed with normal saline (group 1) was filled into the right side, and Tisseel® mixed Bio-Oss® (group 2) was inserted into the other side. The bone mineral density and bone volume were analyzed with microscopic computed tomography (micro-CT) and histomorphometric 12 weeks after application. Results Histologically, new bone formation rate was 14.8%, and grafted bone rate was 70.5% in group 1. In group 2, they were 18.5% and 60.4%, respectively. According to micro-CT analysis, bone mineral density (mg/cm3, BMD) was 2.5% larger in group 1. Conclusions The findings from this study suggest that, although the difference in the bone formation between group 1 and group 2 appears to be insignificant, group 2 had an advantage in using smaller amount of bone substances to achieve the reliable bone formation.

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A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft

Materials ◽

10.3390/ma14092306 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2306

Author(s):

Masaaki Ito ◽

Taku Toriumi ◽

Takahiro Hiratsuka ◽

Hideto Imura ◽

Yasunori Akiyama ◽

...

Keyword(s):

Bone Mineral Density ◽

Bone Formation ◽

Bone Mineral ◽

Bone Volume ◽

Type I ◽

Mineral Density ◽

Recombinant Peptide ◽

Alveolar Cleft ◽

Recombinant Type ◽

Group 2

This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation—and could be useful in human alveolar cleft repairs as well.

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Acupuncture for Treatment of Secondary Osteoporosis in Patients with Spinal Cord Injury: A Controlled Study

Acupuncture in Medicine ◽

10.1136/acupmed-2013-010463 ◽

2014 ◽

Vol 32 (5) ◽

pp. 381-386 ◽

Cited By ~ 11

Author(s):

Qingxi Meng ◽

Xin Liu ◽

Qunqun Shan ◽

Peng Yu ◽

Zhaohu Mao ◽

...

Keyword(s):

Bone Mineral Density ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Combination Therapy ◽

Bone Mineral ◽

Secondary Osteoporosis ◽

Mineral Density ◽

Cord Injury ◽

Group 2 ◽

Group 1

Objective We explored the effect of adjunctive acupuncture on secondary osteoporosis in patients with spinal cord injury (SCI). Methods Patients with subacute SCI were recruited and divided into two groups by patient choice: group 1 patients received standard combination therapy and group 2 patients received combination therapy plus acupuncture for 3 months. The concentrations of IgG, IgM and tumour necrosis factor α (TNFα) in serum and the bone mineral density were measured before and after treatment. Result The decrease in the concentration of TNFα and IgM in patients in group 2 compared with those in group 1 was statistically significant. The IgG level showed no significant change in either group. Bone mineral density increased more after adjunctive acupuncture, but the difference was not significant. Conclusions Further research is needed to determine whether acupuncture as an adjunct to combination therapy can reduce osteoporosis in patients with subacute SCI. Trial Registration Number P153-2008-36

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Effect of Lumbar Spinal Stenosis on Bone Mineral Density in Osteoporosis Patients Treated With Ibandronate

10.21203/rs.3.rs-289834/v1 ◽

2021 ◽

Author(s):

Hyung-Youl Park ◽

Ji-Yoon Ha ◽

Ki-Won Kim ◽

In-Hwa Baek ◽

Soo-Bin Park ◽

...

Keyword(s):

Bone Mineral Density ◽

Spinal Stenosis ◽

Bone Mineral ◽

Lumbar Spinal Stenosis ◽

Negative Correlation ◽

Mineral Density ◽

Total Change ◽

Group 2 ◽

Lumbar Spinal ◽

Group 1

Abstract Background: Lumbar spinal stenosis (LSS) can cause various neurological symptoms and reduce the daily activity of patients. Many studies have shown that free physical activities and exercise can improve bone mineral density (BMD) in patients with osteoporosis. However, the effect of LSS on BMD has not been reported. The purpose of this study was to investigate the effects of LSS on BMD in patients treated with ibandronate for newly diagnosed osteoporosis.Methods: Group 1 included 83 patients with osteoporosis alone, and group 2 included 76 patients with both osteoporosis and symptomatic LSS. We confirmed four BMD values presented as T-score at initial, and 1-, 2-, and 3-year follow-ups. Mean BMD and annual changes of BMD for three years were compared between the two groups. Correlations between initial BMD and total change of BMD were also analyzed in both groups.Results: Mean annual BMDs were significantly higher in group 1 compared than in group 2 (-3.39 vs. -3.58 at 1-year; -3.27 vs. -3.49 at 2-year; -3.13 vs. -3.45 at 3-year; all p < 0.05). Annual change of BMD at 1-year follow-up (0.32 vs. 0.21, p = 0.036) and total change of BMD for three years (0.57 vs. 0.35, p = 0.002) were significantly higher in group 1. Group 1 had a strong negative correlation (r = -0.511, P = 0.000) between initial BMD and total change of BMD, whereas group 2 showed a weak negative correlation (r = -0.247, p = 0.032).Conclusions: Symptomatic LSS may interfere with BMD improvement in the treatment of osteoporosis with ibandronate. Active treatment for LSS with more potent treatment for osteoporosis should be taken to increase BMD for patients with osteoporosis and LSS.

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The relationship between panoramic radiomorphometric indices of the mandible and calcaneus bone mineral density

Medicina ◽

10.3390/medicina46020014 ◽

2010 ◽

Vol 46 (2) ◽

pp. 95 ◽

Cited By ~ 9

Author(s):

Eglė Jagelavičienė ◽

Ričardas Kubilius ◽

Aurelija Krasauskienė

Keyword(s):

Bone Mineral Density ◽

Postmenopausal Women ◽

Bone Mineral ◽

Panoramic Radiography ◽

World Health ◽

Mineral Density ◽

X Ray ◽

Group 2 ◽

The Relationship ◽

Group 1

Objective. The aim of the study was to determine the relationship between bone mineral density in the calcaneus measured using the dual x-ray and laser osteodensitometry technique and bone mineral density in the mandible calculated using the panoramic radiomorphometric indices obtained by applying linear measurements in panoramic radiograms of postmenopausal women. Material and methods. The participants of this study were postmenopausal women (n=129) aged 50 and more. The subjects underwent panoramic radiography of the mandibles, followed by the calculation of the panoramic radiomorphometric indices indicating bone mineral density of the mandible. The dual x-ray and laser osteodensitometer DXL Calscan were used for the measurements of bone mineral density in the calcaneus. Statistical analysis was preformed to find the relationship between bone mineral density measurements in the two anatomically different bones. Results. Following the diagnostic criteria for osteoporosis recommended by the World Health Organization (1994), the subjects were distributed according to the calcaneus bone mineral density T-score into the normal bone mineral density (group 1), osteopenia (group 2), and osteoporosis (group 3) groups. Mean bone mineral density in the calcaneus in the general studied population was 0.38±0.07; the mean value of bone mineral density of the calcaneus in the group 1 (n=34) was 0.47±0.04 (g/cm²), in the group 2 (n=65) was 0.37±0.03 (g/cm²), and in the group 2 (n=30) was 0.29±0.03 (g/cm²). Differences in bone mineral density between the groups were determined using the analysis of variance (ANOVA) F=285.31; df=2; P<0.001 (T1 vs. T2, P<0.001; T1 vs. T3, P<0.001; T2 vs. T3; P<0.001). A statistically significant correlation was found in the general group between the mental index and bone mineral density in the calcaneus (r=0.356, P<0.001), and between the panoramic mandibular index and bone mineral density in the calcaneus (r=0.397, P<0.001). Conclusion. Bone mineral density in the calcaneus and the mandible measured using dual energy x-ray and laser osteodensitometer DXL Calscan and by applying panoramic radiography reflect general changes in the mineralization of these bones, characteristic of the postmenopausal period.

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DINAMIKA MINERAL'NOY PLOTNOSTI KOSTI U RETsIPIENTOV POSLE PERESADKI PEChENI

Osteoporosis and Bone Diseases ◽

10.14341/osteo200922-4 ◽

2009 ◽

Vol 12 (2) ◽

pp. 2-4

Author(s):

I P Ermakova ◽

Ya G Moysyuk ◽

V P Buzulina ◽

T K Koliashvili ◽

E B Yaroshenko ◽

...

Keyword(s):

Bone Mineral Density ◽

Liver Transplantation ◽

Lumbar Spine ◽

Primary Biliary Cirrhosis ◽

Orthotopic Liver Transplantation ◽

Biliary Cirrhosis ◽

Mineral Density ◽

Hip Bmd ◽

Group 2 ◽

Group 1

Axial and periferal bone mineral density (BMD) was estimated by DXA twice within 1-47 months in 16 patients following orthotopic liver transplantation (OLT). Osteoporosis and osteopenia of lumbar spine and hip were identified in the first examination of all 9 recipients (group 1) with primary biliary cirrhosis (PBC) and in 5 out of 7 (71%) patients with viral hepatitic cirrhosis (group 2). Recipients which were firstly examined within 1-7 months following OLT (subgroups 1A, 2A) through 9±2.8 months showed in 1A axial BMD elevation (on 20.7+13.2% ; P=0.024) and in 2A axial so as hip BMD elevation (on 10.5+2.7%;P=0.001 and 8.1+5.9%;P< 0.05). Recipients which were examined later 18 months after OLT demonstrated no increase in BMD (subgroups 1B and 2B). Moreover two of them with PBC showed decrease in BMD, probably, as a result of transplant dysfunction and immunosuppression with glucocorticoids.

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The сhanges of bone mineral density on the risk of progression of osteoarthritis of the knee

Terapevticheskii arkhiv ◽

10.26442/00403660.2019.05.000194 ◽

2019 ◽

Vol 91 (5) ◽

pp. 61-67

Author(s):

N G Kashevarova ◽

E A Taskina ◽

L I Alekseeva ◽

N V Demin ◽

A M Lila ◽

...

Keyword(s):

Bone Mineral Density ◽

Femoral Neck ◽

Bone Mineral ◽

Statistical Significance ◽

Lumbar Vertebrae ◽

Axial Skeleton ◽

Mineral Density ◽

Knee Oa ◽

Group 2 ◽

Group 1

Aim. To find the relationship between bone mineral density (BMD) and risk of knee OA progression in a 5-year prospective study. Materials and methods. 110 females with knee OA were examined twice with 5-year interval. Examination included filling questionnaires, VAS pain assessment, plain knee radiography and axial skeleton densitometry. I stage knee OA was established in 33 (30%) patients, II stage - in 46 (41.8%), III stage - in 26 (23.6%), and IV - in 5 (4.5%). Normal lumbar vertebrae densitometry BMD values were found in 45 patients (40.9%), osteopenia - corresponding BMD values - in 33 (30.0%), and osteoporosis - in 32 (29.1%). Normal femoral neck BMD values were identified in 60 (54.5%) patients, osteopenia - level BMD - in 48 (43.7%), osteoporosis - in 2 (1.8%). In all premenopausal patients (n=15) axial skeleton BMD values were normal. Results. In 5-year interval radiographic progression was established in 40 patients (Group 2), while in 70 (Group 1) patients no progression occurred. Both groups were comparable in terms of age and disease duration, although, more patients from Group 2 tended to have normal baseline densitometry BMD values - both in lumbar vertebrae and femoral neck: 47.5% vs 37.1%, and 62.5% vs 44.3% as compared to Group 1 patients. Patients from Group 1 more often had BMD values corresponding to osteoporosis and osteopenia: 32.9% vs 22.5%, and 55.7% vs 37.5%, respectively, as compared to Group 2 patients, although not achieving statistical significance. These differences were still identifiable after 5-year interval. Absolute BMD values at the second examination in 5 years were indicative of statistically significant increase in femoral neck and total hip BMD in Group 2 patients with knee OA progression: 0.79±0.11 vs 0.73±0.16, р

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Effect of long-term continuous methimazole treatment of hyperthyroidism: comparison with radioiodine

Acta Endocrinologica ◽

10.1530/eje.1.01904 ◽

2005 ◽

Vol 152 (5) ◽

pp. 695-701 ◽

Cited By ~ 72

Author(s):

F Azizi ◽

L Ataie ◽

M Hedayati ◽

Y Mehrabi ◽

F Sheikholeslami

Keyword(s):

Bone Mineral Density ◽

Bone Mineral ◽

Continuous Treatment ◽

Mineral Density ◽

Duration Of Symptoms ◽

Significant Difference ◽

Group 2 ◽

Toxic Goiter ◽

Group 1

Objective: To investigate the long-term effects of continuous methimazole (MMI) therapy. Design and methods: Five hundred and four patients over 40 years of age with diffuse toxic goiter were treated with MMI for 18 months. Within one year after discontinuation of MMI, hyperthyroidism recurred in 104 patients. They were randomized into 2 groups for continuous antithyroid and radioiodine treatment. Numbers of occurrences of thyroid dysfunction and total costs of management were assessed during 10 years of follow-up. At the end of the study, 26 patients were still on continuous MMI (group 1), and of 41 radioiodine-treated patients (group 2), 16 were euthyroid and 25 became hypothyroid. Serum thyroid and lipid profiles, bone mineral density, and echocardiography data were obtained. Results: There was no significant difference in age, sex, duration of symptoms and thyroid function between the two groups. No serious complications occurred in any of the patients. The cost of treatment was lower in group 1 than in group 2. At the end of 10 years, goiter rate was greater and antithyroperoxidase antibody concentration was higher in group 1 than in group 2. Serum cholesterol and low density lipoprotein-cholesterol concentrations were increased in group 2 as compared with group 1; relative risks were 1.8 (1.12–2.95, P < 0.02) and 1.6 (1.09–2.34, P < 0.02) respectively. Bone mineral density and echocardiographic measurements were not different between the two groups. Conclusion: Long-term continuous treatment of hyperthyroidism with MMI is safe. The complications and the expense of the treatment do not exceed those of radioactive iodine therapy.

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Evaluation of Amniotic-Derived Membrane Biomaterial as an Adjunct for Repair of Critical Sized Bone Defects

Advances in Orthopedic Surgery ◽

10.1155/2014/572586 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 9

Author(s):

Mikael Starecki ◽

John A. Schwartz ◽

Daniel A. Grande

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Critical Size ◽

Bone Defects ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

New Bone Formation ◽

Group 3 ◽

Group 2 ◽

Group 1

Introduction. Autogenous bone graft is the gold standard in reconstruction of bone defects. The use of autogenous bone graft is problematic because of limited bone as well as donor site morbidity. This study evaluates a novel biomaterial as an alternative to autogenous bone graft. The biomaterial is amniotic membrane, rich in growth factors. Methods. Twenty-one adult male Sprague-Dawley rats were implanted with biomaterial using the rat critical size femoral gap model. After creation of the critical size femoral gap animals were randomized to one of the following groups: Group 1 (control): gap left empty and received no treatment; Group 2 (experimental): the gap was filled with commercially available bone graft; Group 3 (experimental): the gap was filled with bone graft plus NuCel amniotic tissue preparation. Results. The experimental groups demonstrated new bone formation compared to controls. The results were evident on radiographs and histology. Histology showed Group 1 controls to have 11.1% new bone formation, 37.8% for Group 2, and 49.2% for Group 3. These results were statistically significant. Conclusions. The study demonstrates that amniotic membrane products have potential to provide bridging of bone defects. Filling bone defects without harvesting autogenous bone would provide a significant improvement in patient care.

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Effect of Collagen-Enhanced Fibrin Sealant on Seroma Formation in a Rat Mastectomy Model

Journal of Wound Management and Research ◽

10.22467/jwmr.2020.01242 ◽

2020 ◽

Vol 16 (3) ◽

pp. 137-143

Author(s):

Jong Seong Kim ◽

Pil Seon Eo ◽

Joon Seok Lee ◽

Jeong Woo Lee ◽

Kang Young Choi ◽

...

Keyword(s):

Fibrin Sealant ◽

Three Dimensional ◽

Axillary Lymph ◽

Acetate Solution ◽

Micro Ct ◽

Sprague Dawley ◽

Micro Computed Tomography ◽

Ct Volumetry ◽

Group 2 ◽

Group 1

Background: Seromas are caused by leakage of lymphovascular fluid into postoperative dead space. This is the most common complication after reconstructive breast surgery. The purpose of this study is to demonstrate the utility of seroma-preventing substances by using a collagen-enhanced fibrin sealant on a rat mastectomy model.Methods: Thirty-six Sprague-Dawley rats were divided into three groups. After mastectomy and axillary lymph node dissection, normal saline was applied to the dead spaces in group 1 (control). In group 2, a collagen-enhanced fibrin sealant was applied, and in group 3, triamcinolone acetate solution was applied. Afterwards, the amount of seroma was measured by three-dimensional micro-computed tomography (3D micro-CT) volumetry analysis and manual aspiration after 7 and 14 days, respectively.Results: The volume of seroma were significantly reduced in groups 2 and 3 compared to group 1 in both 3D micro-CT volumetry analysis and manual aspiration on postoperative day 7 (P<0.001). In addition, the results observed in day 14 also showed a decrease in the amount of seroma analyzed by CT in groups 2 and 3 compared to group 1 (P<0.05). In histopathologic examination inflammation was observed more frequently in group 1 and angiogenesis was more active in group 2.Conclusion: The use of a collagen-enhanced fibrin sealant (Collaseal) is as effective as triamcinolone control injected in a rat mastectomy model.

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The effects of one-year simvastatin therapy on women’s bone mineral density

Open Medicine ◽

10.2478/s11536-010-1031-8 ◽

2010 ◽

Vol 5 (5) ◽

pp. 588-592 ◽

Cited By ~ 2

Author(s):

Aleksandar Dimic ◽

Dimitrije Jankovic ◽

Irena Jankovic ◽

Todorka Savic ◽

Nevena Karanovic

Keyword(s):

Risk Factors ◽

Bone Mineral Density ◽

Postmenopausal Women ◽

Bone Mineral ◽

Treated Group ◽

Mineral Density ◽

Simvastatin Therapy ◽

Group 2 ◽

One Year ◽

Group 1

AbstractOnly few studies have reported that bone fracture risk is decreased in hypercholesterolemic postmenopausal women treated with statin therapy. Because of a lack of longitudinal studies on the effect of statins on bones, the aim of our investigation was to estimate the simvastatin therapy effects on bone mineral density in hypercholesterolemic postmenopausal women. Our investigation was carried out on 53 postmenopausal women with hypercholesterolemia. The women included in the study were divided into two groups. Group 1 was comprised of women with two or more (n=32) atherosclerosis risk factors, whereas group 2 had women with less than two (n=21) of these risk factors. All the women included in the study were placed on a hypocholesterolemic diet and the women in group 1 were additionally treated with 20 mg of simvastatin daily. The parameters of lipid status, body mass index, and L2–L4 densitometry were determined at baseline and then after one year. The simvastatin-treated group showed significant improvement of lipid parameters and increased bone mineral density. Finally, changes in bone mineral density between the groups showed significant differences (p<0.05). Although our investigation was carried out on a small group, our results showed a positive effect of the simvastatin therapy on the bone mineral density of postmenopausal women.

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