scholarly journals Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Abdallah E. Mohamed ◽  
Maher Georg Nessim ◽  
Islam Ibrahim Abou-el-seoud ◽  
Khaled Mohamed Darwish ◽  
Abdelaal Shamseldin
Keyword(s):  
16S Rrna ◽  
Tricalcium Phosphate ◽  
Root Zone ◽  
Calcareous Soils ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Highly Efficient ◽  
Northwestern Coast ◽  
Phosphate Solubilizing

Abstract Background Forty phosphate solubilizing bacterial (PSB) isolates were isolated from the root zone of wheat plants cultivated in the Delta and the Northwestern coast regions of Egypt. All isolates proofed their ability to dissolve tricalcium phosphate on the National Botanical Research Institute's phosphate growth medium (NBRIP) by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. They were designated as Egyptian native phosphate solubilizing bacteria (ENPSB). Results All strains proofed their ability to dissolve tricalcium phosphate on (NBRIP) medium by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. The ENPSB 1, 2, and 3 strains were highly efficient as they gave 707, 653, and 693 mg l−1 soluble phosphorus respectively. Intriguingly, the two highly efficient strains for phosphate solubilization were isolated from the Northwestern coast alkaline soils. Moreover, 75% of strains were also produced profitable amounts of indole acetic acid (IAA) ranged from 0.79 to 50.5 mg l−1. Amazingly, the most efficient strain ENPSB 1 in solubilizing phosphorus (707 mg l−1soluble P) was the best one for producing IAA (50.5 mg l−1). The three efficient strains were identified using the sequencing of 16S rRNA. Sequence analysis of 16S rRNA for selected strains confirmed that the strains ENPSB 1, 2, and 3 were genetically closed to Enterobacter aerogenes; Pantoea sp. and Enteriobacter sp. respectively. Conclusion The inoculation by mix cultures of strains (ENPSB 1, 2, and 3) contributed to raising the dry weight and P content of wheat plants by 76% and 12% over the full fertilized plants. Inoculation of soil PSB can be used to solve the deficiency of phosphorus and promote plant growth effectively in calcareous soils.

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from agricultural soils for a potential use in cultivating Capsicum frutescens

2020 ◽  
Vol 10 (2) ◽  
pp. 5161-5173
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Pot Experiment ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Capsicum Frutescens ◽  
Isolation And Characterization ◽  
Insoluble Form ◽  
Phosphate Solubilizing

Phosphorus (P) is one of the essential macronutrients needed for the plant growth, other than nitrogen and potassium. Most phosphorus remains as insoluble form in soils and the plants only can uptake the phosphorus nutrient in soluble forms. Phosphate solubilizing bacteria (PSB) dissolves the phosphorus and make it available for plants. In this study, Soil samples were collected and screened for PSB on PK medium. PSB colonies with the highest phosphate solubilization ability were chosen and used for studying its rhizosphere effect on Capsicum frutescens by pot experiment.. It was evidenced that selected PSB strain could solubilize phosphate in PK medium and modified PK broth. Besides, it provided available phosphorus for plants and enhanced the plant growth in pot experiment.

Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities

2006 ◽  
Vol 34 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Y.P. Chen ◽  
P.D. Rekha ◽  
A.B. Arun ◽  
F.T. Shen ◽  
W.-A. Lai ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Phosphate Solubilizing Bacteria ◽  
Subtropical Soil ◽  
Phosphate Solubilizing

scholarly journals Secretion of Gluconic Acid From Nguyenibacter sp. L1 Is Responsible for Solubilization of Aluminum Phosphate

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Li Li ◽  
Xue Qiang Zhao ◽  
Xiao Ying Dong ◽  
Jian Feng Ma ◽  
Ren Fang Shen
Keyword(s):  
Gluconic Acid ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Aluminum Phosphate ◽  
Available P ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
Major Factors

Phosphorus (P) deficiency is one of the major factors limiting plant growth in acid soils, where most P is fixed by toxic aluminum (Al). Phosphate-solubilizing bacteria (PSBs) are important for the solubilization of fixed P in soils. Many PSBs have been isolated from neutral and calcareous soils, where calcium phosphate is the main P form, whereas PSBs in acid soils have received relatively little attention. In this study, we isolated a PSB strain from the rhizosphere of Lespedeza bicolor, a plant well adapted to acid soils. On the basis of its 16S rRNA gene sequence, this strain was identified as a Nguyenibacter species and named L1. After incubation of Nguyenibacter sp. L1 for 48 h in a culture medium containing AlPO4 as the sole P source, the concentration of available P increased from 10 to 225 mg L–1, and the pH decreased from 5.5 to 2.5. Nguyenibacter sp. L1 exhibited poor FePO4 solubilization ability. When the pH of non-PSB-inoculated medium was manually adjusted from 5.5 to 2.5, the concentration of available P only increased from 6 to 65 mg L–1, which indicates that growth medium acidification was not the main contributor to the solubilization of AlPO4 by Nguyenibacter sp. L1. In the presence of glucose, but not fructose, Nguyenibacter sp. L1 released large amounts of gluconic acid to solubilize AlPO4. Furthermore, external addition of gluconic acid enhanced AlPO4 solubilization and reduced Al toxicity to plants. We conclude that secretion of gluconic acid by Nguyenibacter sp. L1, which is dependent on glucose supply, is responsible for AlPO4 solubilization as well as the alleviation of Al phytotoxicity by this bacterial strain.

scholarly journals Multi-Omics Reveal the Efficient Phosphate-Solubilizing Mechanism of Bacteria on Rocky Soil

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanqiang Ding ◽  
Zhuolin Yi ◽  
Yang Fang ◽  
Sulan He ◽  
Yuming Li ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Agricultural Development ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Fertilizers ◽  
Plant Growth Promoting ◽  
Rocky Soil ◽  
Phosphate Solubilizing

Phosphate-solubilizing bacteria (PSB) can alleviate available phosphorus (AP)-deficiency without causing environmental pollution like chemical phosphate fertilizers. However, the research and application of PSB on the barren rocky soil is very rare. We screened six PSB from sweetpotato rhizosphere rocky soil. Among them, Ochrobactrum haematophilum FP12 showed the highest P-solubilizing ability of 1,085.00 mg/L at 7 days, which was higher than that of the most reported PSB. The assembled genome of PSB FP12 was 4.92 Mb with P-solubilizing and plant growth-promoting genes. In an AP-deficient environment, according to transcriptome and metabolomics analysis, PSB FP12 upregulated genes involved in gluconic acid synthesis and the tricarboxylic acid cycle, and increased the concentration of gluconic acid and malic acid, which would result in the enhanced P-solubilizing ability. Moreover, a series of experiments in the laboratory and field confirmed the efficient role of the screened PSB on significantly increasing AP in the barren rocky soil and promoting sweetpotato yield. So, in this study, we screened highly efficient PSB, especially suitable for the barren rocky soil, and explored the P-solubilizing mechanism. The research will reduce the demand for chemical phosphate fertilizers and promote the environment-friendly agricultural development.

Biodegradation of Tricalcium Phosphate by Phosphate Solubilizing Bacteria

2010 ◽  
Vol 10 (6) ◽  
pp. 531-535 ◽  
Author(s):  
V. Madhan Chakkarava ◽  
R. Arunachala ◽  
S. Vincent ◽  
K. Paulkumar ◽  
G. Annadurai
Keyword(s):  
Tricalcium Phosphate ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

In Vitro Production of Growth Regulator (IAA) and Phosphatase by Phosphate Solubilizing Bacteria

2017 ◽  
Vol 5 (1) ◽  
pp. 32-35 ◽  
Author(s):  
Lalmuankimi Khiangte ◽  
◽  
R. Lalfakzuala
Keyword(s):  
Agar Plate ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
In Vitro Production ◽  
Soil Rhizosphere ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Vitro Production ◽  
Phosphatase Enzyme

The phosphate solubilizing bacteria (phosphobacteria) secrete organic acids and phosphatase enzymes which act on insoluble phosphates and convert the same into soluble forms thus providing available phosphorus to the plants. They are also known to produce amino acids, vitamins and growth promoting substance like indole acetic acid (IAA), which helps in better growth of plants. The present studyaimed to isolate and screen out phosphate solubilizing bacteria from soil rhizosphere using serial dilution technique on pikovskaya agar plate. An effort was also made to obtain bacterial isolates for producing phosphatase enzyme and to study the production of IAA in NBRIP media containing tryptophan as a substrate.A total of 17 strains were isolated from soil rhizosphere and all the strains were able to produce IAA and phosphatase enzyme in vitro condition.

scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut

2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Ella Dewani Larasati ◽  
MG Isworo Rukmi ◽  
Endang Kusdiyantini ◽  
R Cinta Badia Ginting
Keyword(s):  
Growth And Development ◽  
Bacillus Pumilus ◽  
Peat Soil ◽  
Species Level ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Peat Soils ◽  
Biolog System ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification

scholarly journals Isolation and Identification of Elite Phosphate Solubilizing Bacteria from Soil under Paddy Cultivation

2014 ◽  
Vol 16 ◽  
pp. 62-68 ◽  
Author(s):  
A. Gandhi ◽  
G. Muralidharan ◽  
E. Sudhakar
Keyword(s):  
Bacterial Species ◽  
Essential Element ◽  
Broth Culture ◽  
Bacillus Species ◽  
Available Phosphorus ◽  
Growth Media ◽  
Screening Methods ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.

scholarly journals AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL

2016 ◽  
Vol 11 (3) ◽  
pp. 481
Author(s):  
Sri Widawati
Keyword(s):  
Microbial Population ◽  
Farming System ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Environmental Friendliness ◽  
Key Word ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing ◽  
Organic Farming System

The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer

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