scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut

2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Ella Dewani Larasati ◽  
MG Isworo Rukmi ◽  
Endang Kusdiyantini ◽  
R Cinta Badia Ginting
Keyword(s):  
Growth And Development ◽  
Bacillus Pumilus ◽  
Peat Soil ◽  
Species Level ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Peat Soils ◽  
Biolog System ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification

scholarly journals AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL

2016 ◽  
Vol 11 (3) ◽  
pp. 481
Author(s):  
Sri Widawati
Keyword(s):  
Microbial Population ◽  
Farming System ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Environmental Friendliness ◽  
Key Word ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing ◽  
Organic Farming System

The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer

scholarly journals Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Abdallah E. Mohamed ◽  
Maher Georg Nessim ◽  
Islam Ibrahim Abou-el-seoud ◽  
Khaled Mohamed Darwish ◽  
Abdelaal Shamseldin
Keyword(s):  
16S Rrna ◽  
Tricalcium Phosphate ◽  
Root Zone ◽  
Calcareous Soils ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Highly Efficient ◽  
Northwestern Coast ◽  
Phosphate Solubilizing

Abstract Background Forty phosphate solubilizing bacterial (PSB) isolates were isolated from the root zone of wheat plants cultivated in the Delta and the Northwestern coast regions of Egypt. All isolates proofed their ability to dissolve tricalcium phosphate on the National Botanical Research Institute's phosphate growth medium (NBRIP) by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. They were designated as Egyptian native phosphate solubilizing bacteria (ENPSB). Results All strains proofed their ability to dissolve tricalcium phosphate on (NBRIP) medium by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. The ENPSB 1, 2, and 3 strains were highly efficient as they gave 707, 653, and 693 mg l−1 soluble phosphorus respectively. Intriguingly, the two highly efficient strains for phosphate solubilization were isolated from the Northwestern coast alkaline soils. Moreover, 75% of strains were also produced profitable amounts of indole acetic acid (IAA) ranged from 0.79 to 50.5 mg l−1. Amazingly, the most efficient strain ENPSB 1 in solubilizing phosphorus (707 mg l−1soluble P) was the best one for producing IAA (50.5 mg l−1). The three efficient strains were identified using the sequencing of 16S rRNA. Sequence analysis of 16S rRNA for selected strains confirmed that the strains ENPSB 1, 2, and 3 were genetically closed to Enterobacter aerogenes; Pantoea sp. and Enteriobacter sp. respectively. Conclusion The inoculation by mix cultures of strains (ENPSB 1, 2, and 3) contributed to raising the dry weight and P content of wheat plants by 76% and 12% over the full fertilized plants. Inoculation of soil PSB can be used to solve the deficiency of phosphorus and promote plant growth effectively in calcareous soils.

Effect of Phosphate Solubilizing Bacteria on Growth and Development of Pearl Millet and Ragi

2016 ◽  
Vol 7 (3) ◽  
pp. 1-7 ◽  
Author(s):  
B Harinathan ◽  
S Sankaralingam ◽  
S Palpperumal ◽  
D Kathiresan ◽  
T Shankar ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Growth And Development ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

scholarly journals Inorganic and organic phosphate solubilization potential of Stenotrophomonas maltophilia

2021 ◽  
Vol 948 (1) ◽  
pp. 012054
Author(s):  
Suliasih ◽  
S Widawati
Keyword(s):  
Acid Phosphatase ◽  
Stenotrophomonas Maltophilia ◽  
Phosphate Solubilization ◽  
Peat Soil ◽  
Organic Phosphate ◽  
Broth Culture ◽  
Phosphate Solubilizing Bacteria ◽  
Organic P ◽  
Phosphate Solubilizing ◽  
Inorganic And Organic

Abstract Most soils contain Phosphorus (P) in insoluble compounds as organic and inorganic forms which is unavailable to plants. Furthermore, phosphate solubilizing bacteria (PSB) play an important role in converting insoluble P to a bioavailable form through solubilizing and mineralizing inorganic or organic P. Therefore, this study aims to determine the ability of isolate a phosphate solubilizing bacterium (PSB), Stenotrophomonas maltophilia to solubilize inorganic and organic P. The bacterium was isolated from peat soil of West Kalimantan using Pikovskaya medium added with Ca3 (PO4)2 as the P source. The activity of organic P (acid phosphatase and phytase) was measured using p-nitrophenyl phosphate disodium (pNPP 0.115 M) and sodium phytate as the substrate of bacterial broth culture. Also, the optimization for inorganic and organic P solubilization by the bacterium was conducted. The maximum values of inorganic and organic phosphate solubilization was recorded to be 52.26 μg/mL and 44.51 U/mL (acid phosphatase), 0.13 U/mL (phytase) respectively. Optimum conditions found were temperature at 30 °C, pH 6.0 and in the presence of sucrose and beef extract, which serve as carbon and nitrogen sources, respectively. Therefore, it can probably be used as a biological fertilizer for plants.

scholarly journals Indigenous Phosphate Solubilizing Bacteria from Peat Soil for an Eco-friendly Biofertilizer in Oil Palm Plantation

KnE Energy ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 65 ◽  
Author(s):  
Elizabeth Caroline Situmorang ◽  
Andriessa Prameswara ◽  
Hana Christine Sinthya ◽  
Nurita Toruan-Mathius ◽  
Tony Liwang
Keyword(s):  
Renewable Energy ◽  
Soil Fertility ◽  
Oil Palm ◽  
Biochemical Characterization ◽  
Peat Soil ◽  
Essential Element ◽  
Soil Microbiology ◽  
Phosphate Solubilizing Bacteria ◽  
Medium Temperature ◽  
Phosphate Solubilizing

<p>Degradation in soil fertility is an enormous problem in agricultural intensification system. Availability in phosphate is one of among encountered problems. Peat soils have large areas and great potential for agriculture and plantations, but have many obstacles in their management. One of the issues is the low soil fertility and low availability of nutrients, especially phosphate which exists in many organic forms that are not available to plants. Phosphorous is an essential element after nitrogen, which plays an important role in plant growth and metabolism and the process of soil microbiology. Introduction of phosphate- solubilizing bacteria (PSB) as biofertilizer, is a renewable energy based which can increase the availability of phosphate to plants can overcome this problem and at the same time reducing the use of inorganic P fertilizer. Indigenous PSB, isolated from palm oil plantation could be used as a better and friendly bio-agent to improve soil fertility, specifically for phosphate availability. Eight PSB have been isolated from peat soil in oil palm platation. Morphological, biochemical characterization and molecular identifications showed that the 8 isolates belonged to Flavobacterium and Enterobacter genera, more precisely the species of Burkholderia sp and Novosphingobium nitrogenifigens. Characterization of isolates of the highest PSB activity consisted of the ability to dissolve phosphate in Pikovskaya solid medium, temperature resistance optimization and the ability to produce phosphatase enzyme. Results showed that isolate with the highest potentiality of dissolving phosphate formed 3.5 cm of clear zone diameter and the phosphatase activity at 9 days of incubation 5.992 units (μmol / ml / min). The peak of enzyme’s activity was reached at 6 days , which corresponded to 54.782 ppm of phosphorus solubilised. The lowest pH (3.2) was reached at 1st day of incubation. These isolates showed the ability to grow optimally at temperature range of 25 to 300C. Indigenous PSB could be used as biofertilizer agents which are eco friendly.</p><p><strong>Keywords</strong>: indigenous; biofertilizer; renewable energy, phosphate solubilizing bacteria;phosphatase; oil palm</p>

scholarly journals The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean

2018 ◽  
Vol 10 (3) ◽  
pp. 146-150
Author(s):  
LUFITANUR ALFIAH ◽  
DELITA ZUL ◽  
NELVIA NELVIA
Keyword(s):  
Phosphatase Activity ◽  
Soil Phosphorus ◽  
Peat Soil ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Available P ◽  
Available Phosphorus ◽  
Alkaline Soil ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 μg p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.

scholarly journals Potensi bakteri pelarut P dan Penambat N rhizosper kelapa sawit gambut saprik

Jurnal Agro ◽  
10.15575/5776 ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 14-23
Author(s):  
Ida Nur Istina ◽  
Happy Widiastuti ◽  
Benny Joy
Keyword(s):  
Oil Palm ◽  
Symbiotic Nitrogen Fixation ◽  
Soil Depth ◽  
Peat Soil ◽  
N Fixation ◽  
Phosphate Solubilizing Bacteria ◽  
Plate Method ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing ◽  
Biological Fertilizer

Pemanfaatan pupuk hayati sangat berpotensi untuk menurunkan input produksi pada budidaya kelapa sawit khususnya pupuk. Penelitian bertujuan untuk mendapatkan Bakteri Pelarut P dan Penambat N yang berpotensi sebagai bahan pupuk hayati dari rizosfer tanaman kelapa sawit. Penelitian dilakukan di lahan gambut saprik Kabupaten Pelalawan, Provinsi Riau dari bulan Juni sampai November 2014. Pengambilan sampel tanah dilakukan menggunakan bor gambut pada bagian rizosfer dengan kedalaman sampai 20 cm. Isolasi dan karakterisasi dengan metode pure plate menggunakan media selektif N Ashby untuk penambatan N dan Pikovskaya untuk pelarutan P, sedangkan analisis fiksasi N dan pelarutan P dilakukan menggunakan HPLC dan spektrofotometer di Laboratorium mikrobiologi PT. RPN. Hasil penelitian menunjukkan bahwa tanah gambut saprik berpotensi sebagai sumber isolat bakteri pelarut P dan penambat N potensial. Jumlah isolat bakteri pelarut P yang berhasil diperoleh adalah 11 isolat sedangkan jumlah bakteri penambat N non-simbiotik adalah 6 isolat. Isolat bakteri pelarut P potensial asal Sungai Ara dengan kemampuan melarutkan P 329,94 ppm; sedangkan bakteri penambat N non-simbiotik potensial adalah asal Kuala Panduk dengan kemampuan fiksasi N 0,0293 mmol l-1jam-1. ABSTRACTUtilization of biofertilizer is potential to decrease production inputs on oil palm cultivation, especially fertilizer expense. The research aimed to obtain Solubilizing P-Bacteria and Non-Symbiotic Fixing N bacteria which potential as biological fertilizer from oil palm rhyzosphere. The research was conducted at Pelalawan sapric peat soil from June to November 2014. Soil samples were taken by using peat drill into 20 cm soil depth, while isolation and characterization used pure plate method by using the selective media N Ashby for N fixation and Pikovskaya for P solubility. N fixation and P dissolution analyzed by using HPLC and spectrophotometer at PT. RPN microbiology laboratory. The results showed that sapric peat soil potentially utilize as microbial resource. The number of phosphate solubilizing bacteria isolates were 11 isolates, while the number of non-symbiotic nitrogen fixation bacteria inhibiting N Azotobacter sp. were 6 isolates. The potential isolate of P-solubilizing bacteria was Sungai Ara origin with the ability to dissolve P about 329.94 ppm; while the potential of non-symbiotic N-fixing bacteria was Kuala Panduk origin with N fixation ability 0.0293 mmoll-1h-1.

scholarly journals ISOLASI, POPULASI DAN KARAKTERISASI BAKTERI PELARUT FOSFAT PADA DAERAH PERAKARAN DAN TANAH DARI BENGKULU, SUMATRA

2016 ◽  
Vol 13 (1) ◽  
pp. 101
Author(s):  
Sri Purwaningsih
Keyword(s):  
Key Words ◽  
Pure Culture ◽  
Growth Characteristic ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Standard Medium ◽  
Phosphate Solubilizing

Penelitian dilakukan untuk mengetahui isolasi, populasi dan karakterisasi bakteri pelarut fosfat pada tanah dari rizosfer tanaman dari Bengkulu, Sumatra. Tujuan penelitian untuk mengetahui populasi bakteri pelarut fosfat dari rizosfer tanaman, dan mendapatkan kultur murni. Sampel diambil dari 25 sampel tanah dari rizosfer tanaman. Isolasi dilakukan dengan metode dilusi dalam Pycosvkaya menengah standar, inculation di (27-280C) setelah 15 hari, dan penduduk dengan metode plate count. Populasi bakteri pelarut fosfat berada di kisaran 13-101 105 X tanah CFU / g, dan populasi tertinggi ditemukan dari sampel tanah dari rhizoaphere dengan Semangka (Citrulus lanatus) tanaman. Tujuh puluh lima memberikan budaya murni. Karakteristik pertumbuhan kultur murni diamati dengan menggunakan media Pycosvkaya. Inkubasi di (27-280C) setelah 15 hari. Zona jelas mengelilingi koloni menunjukkan bahwa fosfat dalam medium adalah terhidrolisis dan zona terbentuk dari dua hari sampai delapan hari kata kunci: Bakteri pelarut Fosfat, Pycosvkaya, Bengkulu AbstractThe research was conducted in order to know the isolation, population and characterizations of phosphate solubilizing bacteria on the soil from rhizosphere plants from Bengkulu, Sumatra. The purpose of the study to know the population of phosphate solubilizing bacteria from rhizosphere plants, and the get pure culture. The sample wastaken from 25 sample soil from rhizosphere of plants. Isolation was done with dilution methods in standard medium Pycosvkaya, the inculation at (27-280C) after 15 days, and the population with plate count methods. The population of phosphate solubilizing bacteria was in the range of 13-101 X 105 CFU/g soil, and the highest population wasfound from the sample soil from rhizoaphere with Semangka (Citrulus lanatus) plant. Seventy five gave of pure culture. The growth characteristic of pure culture was observed by using Pycosvkaya medium. Incubation at (27-280C) after 15 days. The clear zone surround the colonies indicate that the phosphate in the medium was hydrolyzed and the formed zone from two days until eight days key words: Phosphate Solubilizing Bacteria, Pycosvkaya, Bengkulu 

scholarly journals Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Deni Elfiati ◽  
Delvian DELVIAN ◽  
Hamidah Hanum ◽  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat
Keyword(s):  
Peat Soil ◽  
Selective Medium ◽  
Soil Samples ◽  
Available Phosphorus ◽  
Peat Soils ◽  
Insoluble Phosphate ◽  
Value Range ◽  
Qualitative Test ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.

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