scholarly journals Electric field stimulation for tissue engineering applications

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Christina N. M. Ryan ◽  
Meletios N. Doulgkeroglou ◽  
Dimitrios I. Zeugolis
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Electric Field ◽  
Electric Fields ◽  
Cell Types ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Physiological Processes ◽  
Different Cell Types

AbstractElectric fields are involved in numerous physiological processes, including directional embryonic development and wound healing following injury. To study these processes in vitro and/or to harness electric field stimulation as a biophysical environmental cue for organised tissue engineering strategies various electric field stimulation systems have been developed. These systems are overall similar in design and have been shown to influence morphology, orientation, migration and phenotype of several different cell types. This review discusses different electric field stimulation setups and their effect on cell response.

scholarly journals Cyclodextrin-Polypyrrole Coatings of Scaffolds for Tissue Engineering

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 459 ◽  
Author(s):  
Jan Lukášek ◽  
Šárka Hauzerová ◽  
Kristýna Havlíčková ◽  
Kateřina Strnadová ◽  
Karel Mašek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Conductive Polymers ◽  
Composite Scaffold ◽  
Cell Types ◽  
Hexanoic Acid ◽  
Spectroscopic Techniques ◽  
In Vitro Experiments ◽  
Engineering Applications ◽  
Different Cell Types

Polypyrrole is one of the most investigated conductive polymers used for tissue engineering applications because of its advantageous properties and the ability to promote different cell types’ adhesion and proliferation. Together with β-cyclodextrin, which is capable of accommodating helpful biomolecules in its cavity, it would make a perfect couple for use as a scaffold for tissue engineering. Such scaffolds were prepared by the polymerisation of 6-(pyrrol-3-yl)hexanoic acid on polycaprolactone microfibres with subsequent attachment of β-cyclodextrin on the polypyrrole layer. The materials were deeply characterised by several physical and spectroscopic techniques. Testing of the cyclodextrin enriched composite scaffold revealed its better performance in in vitro experiments compared with pristine polycaprolactone or polypyrrole covered polycaprolactone scaffolds.

Muscarinic receptor subtypes in canine trachea

1990 ◽  
Vol 258 (6) ◽  
pp. L349-L354 ◽  
Author(s):  
J. F. Brichant ◽  
D. O. Warner ◽  
S. J. Gunst ◽  
K. Rehder
Keyword(s):  
Electric Field ◽  
Muscarinic Receptor ◽  
Contractile Response ◽  
Receptor Subtypes ◽  
Field Stimulation ◽  
Muscarinic Receptor Subtypes ◽  
Electric Field Stimulation ◽  
M3 Receptors ◽  
M3 Subtype

Prejunctional and postjunctional muscarinic receptor subtypes were characterized in canine trachealis muscle strips. In vitro contractile responses of muscle strips to acetylcholine or electric field stimulation were determined in the absence and the presence of gallamine, pirenzepine, and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP). Gallamine had no effect on the contractile response to acetylcholine but enhanced the contractile response to electric field stimulation. Pirenzepine and 4-DAMP reduced the contractile response to acetylcholine and electric field stimulation. The pA2 value for pirenzepine vs. acetylcholine [7.18 +/- 0.59 (SD)] was consistent with the affinity of pirenzepine for M2 or M3-receptors; whereas the pA2 value for 4-DAMP vs. acetylcholine (8.92 +/- 0.42) and the extremely low affinity of gallamine indicated postjunctional muscarinic receptors of the M3 subtype. The enhancement of the contractile response to electric field stimulation by gallamine suggested the presence of M2-prejunctional receptors.

Nonneural components in the response of fresh human airways to electric field stimulation

1987 ◽  
Vol 63 (4) ◽  
pp. 1558-1566 ◽  
Author(s):  
J. C. De Jongste ◽  
H. Mons ◽  
I. L. Bonta ◽  
K. F. Kerrebijn
Keyword(s):  
Electric Field ◽  
Time Course ◽  
Adrenergic Nerve ◽  
Organ Bath ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Small Peak ◽  
Antiasthmatic Drug ◽  
Human Bronchi

Fresh human bronchi, obtained at thoracotomy and maintained at 37 degrees C, were studied in vitro to investigate their response to electric field stimulation (EFS). We found complex responses that were not only composed of a rapid initial nerve-mediated cholinergic contraction and a non-adrenergic nerve-mediated relaxation, but, in 80% of preparations, also of a tonic contraction with a sustained time course. This sustained phase was not blocked by the nervous conductance blocker tetrodotoxin (TTX) and was therefore not neurally mediated. Controlled transient cooling to 4 degrees C in the organ bath reduced this sustained phase selectively for several hours. The leukotriene (LT) antagonist FPL 55712, dexamethasone, which inhibits phospholipase A2, and the antiasthmatic drug cromolyn all reduced the sustained phase significantly. In 20% of strips, an additional TTX-resistant contraction was seen directly after the cholinergic phase. This contraction could be inhibited by indomethacin. A similar small peak sometimes appeared after selective blocking of either the cholinergic or the sustained phases. Experiments in which the epithelium was removed from the strips suggested that this indomethacin-sensitive response, but not the sustained phase, was dependent on the presence of epithelium. These results show that EFS of fresh human bronchi stimulated cholinergic and nonadrenergic inhibitory nerves and gave rise to a partly epithelium-dependent synthesis of arachidonic acid metabolites, which caused contractile responses that interfered with the neurally mediated responses.

Microlesion formation in myocardial cells by high-intensity electric field stimulation

1987 ◽  
Vol 253 (2) ◽  
pp. H480-H486 ◽  
Author(s):  
J. L. Jones ◽  
R. E. Jones ◽  
G. Balasky
Keyword(s):  
Electric Field ◽  
High Intensity ◽  
Dielectric Breakdown ◽  
Fluorescein Isothiocyanate ◽  
Dependent Manner ◽  
Field Stimulation ◽  
Myocardial Cells ◽  
Electric Field Stimulation ◽  
Ionic Exchanges

Arrhythmias, S-T segment changes, immediate refibrillation, and other signs of dysfunction are often observed after clinical and experimental transthoracic defibrillation. In vitro studies suggested that shock-induced dysfunction is induced by sarcolemmal dielectric breakdown accompanied by ionic exchanges through transient, shock-induced microlesions in the sarcolemma. To test this hypothesis, cultured chick embryo myocardial cells were shocked in media containing fluorescein isothiocyanate-labeled dextrans (FITC-dextrans) ranging in molecular mass from 4 to 70 kDa, using electric field stimulation 5 ms in duration and ranging in intensity from 0 to 200 V/cm. Results showed that the percentage of cells incorporating 4- to 20-kDa dextrans increased in a dose-dependent manner. The 4- and 10-kDa dextrans were incorporated beginning at intensities of 50–100 V/cm. Dextran incorporation corresponded with shock intensities which produced a shock-induced arrest of spontaneous contraction lasting 1 min. The 20-kDa dextrans were incorporated following 150- and 200-V/cm shocks. Shocks of these intensities also produced a transient postshock contracture. Larger dextrans (40 and 70 kDa) were not incorporated. These results suggest the formation of transient sarcolemmal microlesions having a diameter of 45-60 A during high-intensity electric field stimulation.

scholarly journals Ih interacts with somato-dendritic structure to determine frequency response to weak alternating electric field stimulation

2018 ◽  
Vol 119 (3) ◽  
pp. 1029-1036 ◽  
Author(s):  
Enrique H. S. Toloza ◽  
Ehsan Negahbani ◽  
Flavio Fröhlich
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Cellular Response ◽  
Current Injection ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Cation Current ◽  
Network Oscillations ◽  
Alternating Electric Field

Transcranial current stimulation (tCS) modulates brain dynamics using weak electric fields. Given the pathological changes in brain network oscillations in neurological and psychiatric illnesses, using alternating electric field waveforms that engage rhythmic activity has been proposed as a targeted, network-level treatment approach. Previous studies have investigated the effects of electric fields at the neuronal level. However, the biophysical basis of the cellular response to electric fields has remained limited. Here, we characterized the frequency-dependent response of different compartments in a layer V pyramidal neuron to exogenous electric fields to dissect the relative contributions of voltage-gated ion channels and neuronal morphology. Hyperpolarization-activated cation current (Ih) in the distal dendrites was the primary ionic mechanism shaping the model’s response to electric field stimulation and caused subthreshold resonance in the tuft at 20 ± 4 Hz. In contrast, subthreshold Ih-mediated resonance in response to local sinusoidal current injection was present in all model compartments at 11 ± 2 Hz. The frequencies of both resonance responses were modulated by Ih conductance density. We found that the difference in resonance frequency between the two stimulation types can be explained by the fact that exogenous electric fields simultaneously polarize the membrane potentials at the distal ends of the neuron (relative to field direction) in opposite directions. Our results highlight the role of Ih in shaping the cellular response to electric field stimulation and suggest that the common model of tCS as a weak somatic current injection fails to capture the cellular effects of electric field stimulation. NEW & NOTEWORTHY Modulation of cortical oscillation by brain stimulation serves as a tool to understand the causal role of network oscillations in behavior and is a potential treatment modality that engages impaired network oscillations in disorders of the central nervous system. To develop targeted stimulation paradigms, cellular-level effects must be understood. We demonstrate that hyperpolarization-activated cation current (Ih) and cell morphology cooperatively shape the response to applied alternating electric fields.

Electric Field Stimulation Integrated into Perfusion Bioreactor for Cardiac Tissue Engineering

2010 ◽  
Vol 16 (6) ◽  
pp. 1417-1426 ◽  
Author(s):  
Yiftach Barash ◽  
Tal Dvir ◽  
Pini Tandeitnik ◽  
Emil Ruvinov ◽  
Hugo Guterman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electric Field ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Perfusion Bioreactor ◽  
Field Stimulation ◽  
Electric Field Stimulation

scholarly journals Current concepts: tissue engineering and regenerative medicine applications in the ankle joint

2014 ◽  
Vol 11 (92) ◽  
pp. 20130784 ◽  
Author(s):  
S. I. Correia ◽  
H. Pereira ◽  
J. Silva-Correia ◽  
C. N. Van Dijk ◽  
J. Espregueira-Mendes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Young Patients ◽  
Cell Types ◽  
Ankle Injuries ◽  
Biodegradable Scaffolds ◽  
Functional Regeneration ◽  
High Level ◽  
Different Cell Types

Tissue engineering and regenerative medicine (TERM) has caused a revolution in present and future trends of medicine and surgery. In different tissues, advanced TERM approaches bring new therapeutic possibilities in general population as well as in young patients and high-level athletes, improving restoration of biological functions and rehabilitation. The mainstream components required to obtain a functional regeneration of tissues may include biodegradable scaffolds, drugs or growth factors and different cell types (either autologous or heterologous) that can be cultured in bioreactor systems ( in vitro ) prior to implantation into the patient. Particularly in the ankle, which is subject to many different injuries (e.g. acute, chronic, traumatic and degenerative), there is still no definitive and feasible answer to ‘conventional’ methods. This review aims to provide current concepts of TERM applications to ankle injuries under preclinical and/or clinical research applied to skin, tendon, bone and cartilage problems. A particular attention has been given to biomaterial design and scaffold processing with potential use in osteochondral ankle lesions.

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