A multiplex immunoassay for the non-invasive detection of bladder cancer.

2016 ◽  
Vol 34 (2_suppl) ◽  
pp. 471-471
Author(s):  
Charles Joel Rosser ◽  
Yoshiko Shimizu ◽  
Hideki Furuya ◽  
Peter Bryant-Greenwood ◽  
Owen Chan ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Predictive Value ◽  
Clinical Status ◽  
Multiplex Assay ◽  
Detection Methods ◽  
Protein Biomarkers ◽  
Roc Curve Analysis ◽  
Multiplex Immunoassay ◽  
Non Invasive ◽  
Invasive Test

471 Background: Urine based assays that can non-invasively detect bladder cancer (BCa) have the potential to reduce unnecessary and invasive procedures. The purpose of this study was to develop a multiplex immunoassay that can accurately and simultaneously monitor 10 diagnostic urinary protein biomarkers for application as a non-invasive test for BCa detection Methods: A custom electrochemiluminescent (ECL) multiplex assay was constructed (Meso Scale Diagnostics, LLC, Rockville, MD) to detect the following urinary proteins; IL8, MMP9, MMP10, ANG, APOE, SDC1, A1AT, PAI1, CA9 and VEGFA. Voided urine samples from two cohorts (cohort #1 n = 62 and cohort #2 n = 200) were collected prior to cystoscopy and samples were analyzed blinded to the clinical status of the participants. Means (±SD) and receiver operating characteristic (ROC) curve analysis were used to compare assay performance and to assess the diagnostic accuracy of the diagnostic signature. Results: Comparative diagnostic performance analyses revealed an AUROC value of 0.9258 for the multiplex assay and 0.9467 for the combination of the single-target ELISA assays (p = 0.625), so there was no loss of diagnostic utility for the MSD multiplex assay. Analysis of the independent 200-sample cohort using the multiplex assay achieved an overall diagnostic sensitivity of 0.85, specificity of 0.81, positive predictive value 0.82 and negative predictive value 0.84. Conclusions: It is technically feasible to simultaneously monitor complex urinary diagnostic signatures in a single assay without loss of performance. The described protein-based assay has the potential to be developed for the non-invasive detection of BCa.

Using microRNA profiling in urine samples to develop a non-invasive test for bladder cancer

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Lourdes Mengual ◽  
Juan José Lozano ◽  
Mercedes Ingelmo-Torres ◽  
Cristina Gazquez ◽  
María José Ribal ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urine Samples ◽  
Non Invasive ◽  
Invasive Test ◽  
Microrna Profiling

The Role of Musculoskeletal USG as Diagonostic Tool of CTS

2014 ◽  
Vol 25 (2) ◽  
pp. 44-49
Author(s):  
P Das ◽  
R Pramanik ◽  
M Kataruka ◽  
RN Haldar ◽  
S Samanta ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Predictive Value ◽  
Low Cost ◽  
Large Population ◽  
Study Data ◽  
Cross Sectional ◽  
Non Invasive ◽  
Invasive Test ◽  
Kappa Value

Abstract Introduction Over the years NCS has been used to diagnose and monitor the patients with CTS though USG has several advantages as diagnostic tool. This study has been done to find out the diagnostic accuracy of USG in CTS and to compare the efficacy of USG with standard NCS in CTS. Prospective cross-sectional analytical study was conducted at Dept. of PM&R, IPGME&R, Kolkata from 1st March, 2012 to 31st August 2012 (6 months). Patient with clinical diagnosis of CTS of age >18 year of both sexes were included in this study and on the other hand patient with previous wrist surgery /injury, wrist deformity, diabetes mellitus, anatomical variants of median nerve on ultrasound were excluded from the study. Methodology After getting institutional ethical committee clearance, all patients who fulfil the above criteria were included in the study and further diagnostic conformation done by the standard diagnostic criteria of NCS. The same group of patients have been also screened by ultrasonography (USG). Results At the end of the study, data analysis showed that sensitivity and specificity were 92.3 and 70.0% respectively. Predictive value of +test, predictive value of -test were 88.9 and 77.8% respectively. Kappa value was 0.64 (between 0.5 and 0.7). It signifies that there is good correlation between NCS and USG as diagnostic tool of CTS. The comparison of the numerical values of median latency, amplitude and CSA USG within the groups with the help of ANOVA followed by Tukey's test showed that there was good correlation between latency and amplitude in mild, moderate, severe and profound CTS but unfortunately it was not correlated with the CSA measured by USG. Conclusion USG can be used for screening large population of patients as it is simple, easily available, non-invasive test and has relatively low cost and useful in evaluating and excluding local causes of nerve compression.

236 Using microRNA profiling in urine samples to develop a non-invasive test for bladder cancer

2013 ◽  
Vol 12 (1) ◽  
pp. e236-e237
Author(s):  
L. Mengual ◽  
J.J. Lozano ◽  
M. Ingelmo-Torres ◽  
C. Gazquez ◽  
M.J. Ribal ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urine Samples ◽  
Non Invasive ◽  
Invasive Test ◽  
Microrna Profiling

scholarly journals A multiplex immunoassay for the non-invasive detection of bladder cancer

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Yoshiko Shimizu ◽  
Hideki Furuya ◽  
Peter Bryant Greenwood ◽  
Owen Chan ◽  
Yunfeng Dai ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Multiplex Immunoassay ◽  
Non Invasive

Diagnosis of Deep Vein Thrombosis by Combination of Doppler Ultrasound Flow Examination and Strain Gauge Plethysmography

1982 ◽  
Vol 47 (02) ◽  
pp. 141-144 ◽  
Author(s):  
H Bounameaux ◽  
B Krähenbühl ◽  
S Vukanovic
Keyword(s):  
Deep Vein Thrombosis ◽  
Negative Predictive Value ◽  
Doppler Ultrasound ◽  
Strain Gauge ◽  
Predictive Value ◽  
Lower Limbs ◽  
Vein Thrombosis ◽  
Non Invasive ◽  
Strain Gauge Plethysmography ◽  
Deep Vein

SummaryDoppler ultrasound flow examination, strain gauge plethysmography and contrast venography were performed in 160 lower limbs of 80 in-patients. Deep vein thrombosis (DVT) was suspected in 87 limbs. Using measurement of venous stop-flow pressure, the Doppler method had an overall sensitivity of 83%. By combined use of Doppler and Plethysmography, sensitivity was increased to 96%. Specificity was 62% and 51%, respectively. With a positive and a negative predictive value of 80% and 73%, respectively, the combination of both non-invasive methods cannot reliably replace venography in the diagnosis of DTV, although all (40/40) thromboses proximal to or involving the popliteal segment were detected by either Doppler and Plethysmography or both.After exclusion of 14 patients (18%) suffering from conditions known to alter the results of these non-invasive methods, the positive predictive value of abnormal findings in both Doppler and Plethysmography was increased to 94% for suspected limbs, whilst negative predictive value of both negative Doppler and Plethysmography was 90%, allowing the avoidance of venography in these patients.

Implementation of a novel non-invasive test for monitoring control in individuals with congenital adrenal hyperplasia

2018 ◽  
Author(s):  
Irina Bacila ◽  
Carlo L Acerini ◽  
Ruth E Krone ◽  
Leena Patel ◽  
Sabah Alvi ◽  
...  
Keyword(s):  
Congenital Adrenal Hyperplasia ◽  
Adrenal Hyperplasia ◽  
Non Invasive ◽  
Invasive Test

Noninvasive Assessment of Liver Diseases using 2D Shear Wave Elastography

2016 ◽  
Vol 25 (4) ◽  
pp. 525-532 ◽  
Author(s):  
Monica Lupșor-Platon ◽  
Radu Badea ◽  
Mirela Gersak ◽  
Anca Maniu ◽  
Ioana Rusu ◽  
...  
Keyword(s):  
Shear Wave ◽  
Predictive Value ◽  
Liver Diseases ◽  
Venous Pressure ◽  
Liver Stiffness ◽  
Shear Wave Elastography ◽  
Acoustic Radiation Force Impulse ◽  
Two Dimensional ◽  
Non Invasive ◽  
Examination Technique

There has been great interest in the development of non-invasive techniques for the diagnosis of liver fibrosis in chronic liver diseases, including ultrasound elastographic methods. Some of these methods have already been adequately studied for the non-invasive assessment of diffuse liver diseases. Others, however, such as two-dimensional Shear Wave Elastography (SWE), of more recent appearance, have yet to be validated and some aspects are for the moment incompletely elucidated. This review discusses some of the aspects related to two-dimensional SWE: the examination technique, the examination performance indicators, intra and interobserver agreement and clinical applications. Recommendations for a high-quality examination technique are formulated. Key words:  –  –  – Two-dimensional Shear Wave Elastography. Abbreviations: 2D- SWE: Two-dimensional Shear Wave Elastography; 3D- SWE: Three-dimensional Shear Wave Elastography; AUROC: area under the receiver operating characteristic curves; ARFI Acoustic Radiation Force Impulse Elastography; EFSUMB: European Federation of Societies for Ultrasound in Medicine and Biology; HVPG: hepatic venous pressure gradient; LS: liver stiffness; LR: likelihood ratio; NPV: negative predictive value; PPV: positive predictive value; ROI: region of interest; RT-E: Real Time-Elastography; Se: sensitivity; Sp: specificity; TE: Transient Elastography; US: ultrasound; VM: valid measurement; E: Young’s modulus

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