A Biotechnological Approach to Formulate Vaccines Within Liposomes: Immunization Studies

2006 ◽  
pp. 413-430
Author(s):  
Cristián Raziel Delgado-González ◽  
Alfredo Madariaga-Navarrete ◽  
José Miguel Fernández-Cortés ◽  
Margarita Islas-Pelcastre ◽  
Goldie Oza ◽  
...  

Potable and good-quality drinking water availability is a serious global concern, since several pollution sources significantly contribute to low water quality. Amongst these pollution sources, several are releasing an array of hazardous agents into various environmental and water matrices. Unfortunately, there are not very many ecologically friendly systems available to treat the contaminated environment exclusively. Consequently, heavy metal water contamination leads to many diseases in humans, such as cardiopulmonary diseases and cytotoxicity, among others. To solve this problem, there are a plethora of emerging technologies that play an important role in defining treatment strategies. Phytoremediation, the usage of plants to remove contaminants, is a technology that has been widely used to remediate pollution in soils, with particular reference to toxic elements. Thus, hydroponic systems coupled with bioremediation for the removal of water contaminants have shown great relevance. In this review, we addressed several studies that support the development of phytoremediation systems in water. We cover the importance of applied science and environmental engineering to generate sustainable strategies to improve water quality. In this context, the phytoremediation capabilities of different plant species and possible obstacles that phytoremediation systems may encounter are discussed with suitable examples by comparing different mechanistic processes. According to the presented data, there are a wide range of plant species with water phytoremediation potential that need to be studied from a multidisciplinary perspective to make water phytoremediation a viable method.


2010 ◽  
Vol 43 (1/2/3) ◽  
pp. 251 ◽  
Author(s):  
Asha A. Juwarkar ◽  
Santosh Kumar Yadav ◽  
Prashant R. Thawale

Author(s):  
Ravi Shankar Singh ◽  
Tirthartha Chattopadhyay ◽  
Dharamsheela Thakur ◽  
Nitish Kumar ◽  
Tribhuwan Kumar ◽  
...  

2007 ◽  
Vol 37 (4) ◽  
pp. 309-321 ◽  
Author(s):  
Robert Arnell ◽  
Rickard Johannisson ◽  
Johan Lindholm ◽  
Torgny Fornstedt ◽  
Bo Ersson ◽  
...  

ChemSusChem ◽  
2017 ◽  
Vol 10 (3) ◽  
pp. 562-574 ◽  
Author(s):  
Ksenia N. Sorokina ◽  
Oxana P. Taran ◽  
Tatiana B. Medvedeva ◽  
Yuliya V. Samoylova ◽  
Alexandr V. Piligaev ◽  
...  

2007 ◽  
Vol 2 (8) ◽  
pp. 1014-1025 ◽  
Author(s):  
Arthur R. Binupriya ◽  
Muthuswamy Sathishkumar ◽  
Kavitha Dhamodaran ◽  
Rasu Jayabalan ◽  
Krishnaswamy Swaminathan ◽  
...  

2017 ◽  
Vol 2 (6) ◽  
pp. 599 ◽  
Author(s):  
Tifa R. Kusumastuti ◽  
Rizkita R. Esyantia ◽  
Fenny M. Dwivany

Banana is one of the major fruit crops, though its conventional breeding has limitations, such as sterility and high polyploidy  levels.  Biotechnological  approach  using genetic  transformation  crop for improvement  offers  an alternative  solution.  In  this  study  a  protocol  was developed  for  establishing genetic  transformation  from embryogenic callus and somatic embryos of the banana cv Ambon Lumut . Embryogenic callus was obtained in ID4 medium (MS-based medium) supplemented with 1 mg L-1 IAA, 4 mg L-1 2,4D, and 0.03 g L-1 active charcoal. Embryogenic callus was transferred into liquid mediu m to establish somatic embryos. Embryogenic callus and somatic embryos were used for Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain A GL1, containing pART-TEST7 p lasmid with gfp gene as a reporter and CaM V35S as a promoter, was used for transformations. The embryogenic callus and somatic embryos were transformed using heat-shock method followed by centrifugation  (2000 rpm) and co-cult ivation in liquid medium containing acetosyringone (100 M) for 3 days. Results of the GFP analysis showed transient expression from gfp gene reporter in transformed embryogenic callus and somatic embryos. Transformation efficiency in somatic embryos (85,9%) was higher than  that in embryogenic callus (32.09%). PCR analysis using CaMV primer showed bands that compatible with CaMV35S promoter at 507 bp. This is a report showing establisment of embryogenic callus and somatic embryo culture transformation by using A. tumefaciens-mediated transformation protocol of the local banana cv Ambon Lumut. This study proved  the huge potential for genetic transformation of banana cv Ambon Lumut for crop improvement, such as pest or disease  resistance and abiotic factor stress tolerance. Keywords: banana; embryogenic callus; somatic embryos.


Botany ◽  
2021 ◽  
Author(s):  
Roudy Jean ◽  
Damase Khasa ◽  
Stéphane Boudreau

Plants maintain beneficial mutualistic relationships with the mycobiont communities found in their rhizosphere, leading to an increase in plant productivity and health. In nutrient-depleted substrates like mine tailings, mycobiont inoculation is often recommended to help restore a successful plant cover. Our 15-week greenhouse experiment aimed to assess the individual effects of a dark septate endophyte (Phialocephala fortinii #4; KX611529) and two Helotiales strains (Rhizoscyphus ericae #22; EU221877 and Meliniomyces sp #1; KT275679) on the growth of Salix planifolia cuttings on sterilized and unsterilized waste rock. Rhizoscyphus ericae increased cuttings shoot biomass on sterilized waste rock while Meliniomyces sp had a positive effect for cuttings grown on unsterilized waste rock. However, P. fortinii strain had no effect on the survival rate, shoot production, and biomass production of S. planifolia cuttings. This study demonstrates that controlled inoculation with ecologically well-adapted mycobionts could promote plant establishment and productivity on abandoned waste rock and be an efficient and integrated biotechnological approach for ecological restoration of canadian mining boreal ecosystems.


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