Production of Extracellular Polymeric Substances from Lactic Acid Bacteria: Their Antimicrobial Effect and Potential Application in Food Industry

2021 ◽  
Vol 924 (1) ◽  
pp. 012079
Author(s):  
R H Muslimah ◽  
T Mahatmanto ◽  
J Kusnadi ◽  
U Murdiyatmo

Abstract Fructophilic lactic acid bacteria are a group of newly discovered lactic acid bacteria. Despite their potential application as probiotics in the food industry, exploration of ecological niches to discover new fructophilic lactic acid bacteria is scarce, and information that concisely describes the practical aspects of their discovery process is limited. In this mini-review, we focus on methods that have been developed to discover fructophilic lactic acid bacteria from fructose-rich environments such as flowers and bee products. First, we briefly introduce the definition, classification, diversity, and ecological niches of fructophilic lactic acid bacteria. Next, we discuss the unique characteristics that distinguish fructophilic lactic acid bacteria from other microorganisms. Finally, we outline the principles and steps to isolate, characterize, select, and identify fructophilic lactic acid bacteria. The discovery of fructophilic lactic acid bacteria with unique characteristics could provide an impetus for the development of probiotics from fructophilic lactic acid bacteria.


2012 ◽  
Vol 167 (5) ◽  
pp. 1314-1324 ◽  
Author(s):  
Jayakumar Beena Divya ◽  
Kontham Kulangara Varsha ◽  
Kesavan Madhavan Nampoothiri

2021 ◽  
pp. 55-61
Author(s):  
Natalia S. Brizuela ◽  
Liliana C. Semorile ◽  
Bárbara M. Bravo-Ferrada ◽  
Emma Elizabeth Tymczyszyn

2016 ◽  
Vol 54 ◽  
pp. 72-79 ◽  
Author(s):  
Verónica Romero-Gil ◽  
Pedro García-García ◽  
Antonio Garrido-Fernández ◽  
Francisco Noé Arroyo-López

2003 ◽  
Vol 69 (3) ◽  
pp. 1797-1799 ◽  
Author(s):  
Torben Lüders ◽  
Gunn Alice Birkemo ◽  
Gunnar Fimland ◽  
Jon Nissen-Meyer ◽  
Ingolf F. Nes

ABSTRACT The antimicrobial effect obtained upon combining the prokaryotic antimicrobial peptides (AMPs; more commonly referred to as bacteriocins) pediocin PA-1, sakacin P, and curvacin A (all produced by lactic acid bacteria [LAB]) with the eukaryotic AMP pleurocidin (from fish) has been investigated. The three LAB AMPs alone were active against gram-positive Listeria ivanovii bacteria at nanomolar concentrations, whereas they were inactive against gram-negative Escherichia coli bacteria. Pleurocidin alone was active against both of these types of bacteria at micromolar concentrations. Little if any synergy between the LAB AMPs and pleurocidin against the gram-positive L. ivanovii strain was obtained. In contrast, the LAB AMPs and pleurocidin acted highly synergistically against the gram-negative E. coli strain. Nanomolar concentrations of LAB AMPs increased the growth inhibitory potency of pleurocidin by about fourfold. When micromolar concentrations of LAB AMPs were combined with 2 μg of pleurocidin/ml, 100% growth inhibition was attained, whereas pleurocidin alone at a concentration of 2 μg/ml gave no growth inhibition. Most noteworthy, when high concentrations (128 μg/ml) of pleurocidin in the absence of LAB AMPs were used over a long period of incubation (1 week), some growth of E. coli was observed, whereas 16 μg of pleurocidin/ml completely abolished growth in the presence of 64 to 128 ng of LAB AMPs/ml over the same period of time. The results clearly demonstrate that combining eukaryotic and prokaryotic AMPs can greatly increase the specific activity and broaden the target-cell range of these peptides.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3431
Author(s):  
Daria Zamojska ◽  
Adriana Nowak ◽  
Ireneusz Nowak ◽  
Ewa Macierzyńska-Piotrowska

Since 2006, the use of growth-promoting antibiotics has been banned throughout the European Union. To meet the expectations of livestock farmers, various studies have been carried out with the use of lactic acid bacteria. Scientists are trying to obtain the antimicrobial effect against the most common pathogens in large-scale farms. Supplementing the diet of broilers with probiotics (live, nonpathogenic microorganisms) stabilized the intestinal microbiota, which improved the results of body weight gain (BWG) and feed intake (FI). The positive effect of probiotics based on lactic acid bacteria has been shown to prevent the occurrence of diarrhea during piglet weaning. The antagonistic activity of postbiotics (inanimate bacteria, cell components, or post-fermentation by-products) from post-culture media after lactobacilli cultures has been proven on Staphylococcus aureus—the pathogen most often responsible for causing mastitis among dairy cows. The article aims to present the latest research examining the antagonistic effect of lactic acid bacteria on the most common pathogens in broilers, piglets, pigs, and cow farms.


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