Detection of globoderosis foci, especially in the early stages of land settlement, is difficult, as typical signs of damage (growth retardation, chlorosis, leaf wilting, etc.) appear visually only after many years of cultivation of nightshade crops in monoculture or with minimal breaks. Under such conditions, the highest probability of their detection is provided by the selection of soil samples by the envelope method. At the same time, in comparison with other monitoring schemes, a more uniform coverage of the surveyed area is achieved. When conducting rapid analysis of moist soil, to increase the secretion of cysts, you should use a saturated solution of NaCl salt at the rate of 50 grams per 1 liter of water. Identification of cyst-forming potato nematodes should be carried out according to a set of morphometric parameters, biological features (using varieties-differentiators) and biochemical differences. Given the current high cost of biochemical methods of diagnosis, it is advisable to use them primarily to identify quarantine and new to science species of cyst-forming nematodes.
Rapid Analysis of 2,4-D in Soil Samples by Modified Soxhlet Apparatus Using HPLC with UV Detection
