Poly(Vinyl Alcohol) Cryogel Membranes Loaded with Resveratrol as Potential Active Wound Dressings

AbstractHydrogel wound dressings are highly effective in the therapy of wounds. Yet, most of them do not contain any active ingredient that could accelerate healing. The aim of this study was to prepare hydrophilic active dressings loaded with an anti-inflammatory compound - trans-resveratrol (RSV) of hydrophobic properties. A special attention was paid to select such a technological strategy that could both reduce the risk of irritation at the application site and ensure the homogeneity of the final hydrogel. RSV dissolved in Labrasol was combined with an aqueous sol of poly(vinyl) alcohol (PVA), containing propylene glycol (PG) as a plasticizer. This sol was transformed into a gel under six consecutive cycles of freezing (-80 °C) and thawing (RT). White, uniform and elastic membranes were successfully produced. Their critical features, namely microstructure, mechanical properties, water uptake and RSV release were studied using SEM, DSC, MRI, texture analyser and Franz-diffusion cells. The cryogels made of 8 % of PVA showed optimal tensile strength (0.22 MPa) and elasticity (0.082 MPa). The application of MRI enabled to elucidate mass transport related phenomena in this complex system at the molecular (detection of PG, confinement effects related to pore size) as well as at the macro level (swelling). The controlled release of RSV from membranes was observed for 48 h with mean dissolution time of 18 h and dissolution efficiency of 35 %. All in all, these cryogels could be considered as a promising new active wound dressings.

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Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

Polymers ◽

10.3390/polym13132104 ◽

2021 ◽

Vol 13 (13) ◽

pp. 2104

Author(s):

Sibusiso Alven ◽

Blessing Atim Aderibigbe

Keyword(s):

Mechanical Properties ◽

Wound Healing ◽

Vinyl Alcohol ◽

Wound Dressing ◽

Chronic Wounds ◽

Cellular Adhesion ◽

Wound Dressings ◽

Poly Vinyl Alcohol ◽

High Porosity ◽

Hybrid Nanofibers

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

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Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract

Molecules ◽

10.3390/molecules26010083 ◽

2020 ◽

Vol 26 (1) ◽

pp. 83

Author(s):

Cláudia Mouro ◽

Colum P. Dunne ◽

Isabel C. Gouveia

Keyword(s):

Composite Material ◽

Vinyl Alcohol ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Coating Layer ◽

Wound Dressings ◽

Poly Vinyl Alcohol ◽

Microbial Invasion ◽

Layer Composite ◽

Medicinal Plant Extracts

Wounds display particular vulnerability to microbial invasion and infections by pathogenic bacteria. Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents. Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects. In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG). This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO). The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity. Moreover, antibacterial assays showed that AG-incorporated into PVA_CS’s coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth. Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility. In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.

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The Design, Characterization and Antibacterial Activity of Heat and Silver Crosslinked Poly(Vinyl Alcohol) Hydrogel Forming Dressings Containing Silver Nanoparticles

Nanomaterials ◽

10.3390/nano11010096 ◽

2021 ◽

Vol 11 (1) ◽

pp. 96

Author(s):

John Jackson ◽

Helen Burt ◽

Dirk Lange ◽

In Whang ◽

Robin Evans ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Silver Nitrate ◽

Vinyl Alcohol ◽

Unmet Need ◽

Chemical Characterization ◽

Wound Dressings ◽

Poly Vinyl Alcohol ◽

Burn Wound

The prompt treatment of burn wounds is essential but can be challenging in remote parts of Africa, where burns from open fires are a constant hazard for children and suitable medical care may be far away. Consequently, there is an unmet need for an economical burn wound dressing with a sustained antimicrobial activity that might be manufactured locally at low cost. This study describes and characterizes the novel preparation of a silver nitrate-loaded/poly(vinyl alcohol) (PVA) film. Using controlled heating cycles, films may be crosslinked with in situ silver nanoparticle production using only a low heat oven and little technical expertise. Our research demonstrated that heat-curing of PVA/silver nitrate films converted the silver to nanoparticles. These films swelled in water to form a robust, wound-compatible hydrogel which exhibited controlled release of the antibacterial silver nanoparticles. An optimal formulation was obtained using 5% (w/w) silver nitrate in PVA membrane films that had been heated at 140 °C for 90 min. Physical and chemical characterization of such films was complemented by in vitro studies that confirmed the effective antibacterial activity of the released silver nanoparticles against both gram positive and negative bacteria. Overall, these findings provide economical and simple methods to manufacture stable, hydrogel forming wound dressings that release antibiotic silver over prolonged periods suitable for emergency use in remote locations.

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Papain wound dressings obtained from poly(vinyl alcohol)/calcium alginate blends as new pharmaceutical dosage form: Preparation and preliminary evaluation

European Journal of Pharmaceutics and Biopharmaceutics ◽

10.1016/j.ejpb.2016.12.001 ◽

2017 ◽

Vol 113 ◽

pp. 11-23 ◽

Cited By ~ 22

Author(s):

J.A.P. Dutra ◽

S.G. Carvalho ◽

A.C.D. Zampirolli ◽

R.D. Daltoé ◽

R.M. Teixeira ◽

...

Keyword(s):

Vinyl Alcohol ◽

Calcium Alginate ◽

Dosage Form ◽

Wound Dressings ◽

Poly Vinyl Alcohol ◽

Preliminary Evaluation ◽

Pharmaceutical Dosage Form

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Chitosan-coated poly(vinyl alcohol) nanofibers for wound dressings

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.31554 ◽

2009 ◽

Vol 9999B ◽

pp. NA-NA ◽

Cited By ~ 11

Author(s):

Yun Ok Kang ◽

In-Soo Yoon ◽

So Young Lee ◽

Dae-Duk Kim ◽

Seung Jin Lee ◽

...

Keyword(s):

Vinyl Alcohol ◽

Wound Dressings ◽

Poly Vinyl Alcohol

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Medicated wound dressings based on poly(vinyl alcohol)/poly(N-vinyl pyrrolidone)/chitosan hydrogels

Journal of Applied Polymer Science ◽

10.1002/app.23344 ◽

2006 ◽

Vol 101 (4) ◽

pp. 2453-2463 ◽

Cited By ~ 40

Author(s):

Haijun Yu ◽

Xiaoyi Xu ◽

Xuesi Chen ◽

Jingquan Hao ◽

Xiabin Jing

Keyword(s):

Vinyl Alcohol ◽

Wound Dressings ◽

Vinyl Pyrrolidone ◽

Poly Vinyl Alcohol ◽

Chitosan Hydrogels

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Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model

Acta veterinaria ◽

10.2478/acve-2021-0025 ◽

2021 ◽

Vol 71 (3) ◽

pp. 285-302

Author(s):

Tijana Lužajić Božinovski ◽

Vera Todorović ◽

Ivan Milošević ◽

Vladimir Gajdov ◽

Bogomir Bolka Prokić ◽

...

Keyword(s):

Soft Tissue ◽

Vinyl Alcohol ◽

International Standards ◽

Wound Dressings ◽

Poly Vinyl Alcohol ◽

Type Iii Collagen ◽

Type I ◽

Subcutaneous Implantation ◽

Ki 67

Abstract A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.

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