scholarly journals Serum Retinol-Binding Protein 4 Is Reduced after Weight Loss in Morbidly Obese Subjects

2007 ◽  
Vol 92 (3) ◽  
pp. 1168-1171 ◽  
Author(s):  
Dominik G. Haider ◽  
Karin Schindler ◽  
Gerhard Prager ◽  
Arthur Bohdjalian ◽  
Anton Luger ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Weight Loss ◽  
Gastric Banding ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Morbidly Obese ◽  
Model Assessment ◽  
Serum Concentrations ◽  
Retinol Binding Protein 4 ◽  
Obese Subjects

Abstract Context: Administration of retinol-binding protein 4 (RBP-4) impairs insulin sensitivity in animals, and elevated serum concentrations have been associated with insulin resistance in humans. Objective: We have studied whether weight loss influences RBP-4. Patients and Methods: Fasting serum concentrations of RBP-4 were measured before and 6 months after gastric banding surgery in 33 morbidly obese patients aged 40 ± 11 yr with a body mass index (BMI) of 46 ± 5 kg/m2. Fourteen healthy subjects aged 29 ± 5 yr with a BMI less than 25 kg/m2 served as controls. To characterize the association of weight loss with central and peripheral appetite regulation, the signaling protein agouti-related protein (AGRP), the orexigenic hormone ghrelin, and its recently identified antagonist obestatin were determined. Results: At baseline, RBP-4 levels were markedly higher in obese than in lean subjects (2.7 ± 0.5 vs. 0.9 ± 0.5 μg/ml; P < 0.001). In contrast, AGRP and obestatin were lower in obese subjects compared with lean controls (all P < 0.001). Six months after gastric banding, BMI was reduced to 40 ± 5 kg/m2, RBP-4 was reduced to 2.0 ± 0.7 μg/ml, AGRP increased from 1.8 ± 1.1 to 3.4 ± 1.1 ng/ml, ghrelin increased from 93 ± 58 to 131 ± 70 pg/ml, and obestatin increased from 131 ± 52 to 173 ± 35 pg/ml (all P < 0.05). Individual changes of RBP-4 were associated with changes of BMI (r = 0.72), the homeostasis model assessment insulin resistance-index (r = 0.53), and total cholesterol (r = 0.42, for all P < 0.05). Conclusion: Reductions in circulating RBP-4 may contribute to improved insulin resistance in morbidly obese subjects after weight loss. This is accompanied by favorable changes in appetite-regulating hormones, which might support the sustained weight loss after obesity surgery.

scholarly journals Associations between Tissue Visfatin/Nicotinamide, Phosphoribosyltransferase (Nampt), Retinol Binding Protein-4, and Vaspin Concentrations and Insulin Resistance in Morbidly Obese Subjects

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Zeynep Goktas ◽  
Shannon Owens ◽  
Mallory Boylan ◽  
David Syn ◽  
Chwan-Li Shen ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Adipose Tissue ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Morbidly Obese ◽  
Tissue Samples ◽  
Blood Concentrations ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Retinol Binding Protein 4 ◽  
Obese Subjects

Visfatin/Nampt, vaspin, and retinol binding protein-4 (RBP-4) play an important role in insulin resistance. The objectives of this study were to measure visfatin/Nampt, vaspin, and RBP-4 concentrations in blood, liver, muscle, subcutaneous, omental, and mesenteric adipose tissues in morbidly obese subjects and investigate their relationship to insulin resistance. Blood and tissue samples were collected from 38 morbidly obese subjects during Roux-en-Y surgery. Insulin resistance biomarkers were measured using standard kits. Visfatin/Nampt, vaspin, and RBP-4 gene expression levels in tissues were measured using real-time PCR. Their protein concentrations in blood and tissues were measured using ELISA kits. Diabetic subjects had significantly higher homeostasis model of assessment-insulin resistance and age and lower blood HDL-cholesterol concentrations than nondiabetic and prediabetic subjects. Diabetic and prediabetic subjects had significantly higher blood concentrations of visfatin/Nampt and vaspin than nondiabetic subjects. Liver RBP-4 concentrations were positively associated with blood glucose concentrations. Blood insulin resistance biomarker levels were positively associated with visfatin/Nampt concentrations in omental adipose tissue and liver, and vaspin concentrations in mesenteric adipose tissue. In conclusion, the correlations of visfatin/Nampt, vaspin, and RBP-4 with insulin resistance are tissue dependent.

scholarly journals Retinol-Binding Protein 4 and Lipocalin-2 in Childhood and Adolescent Obesity: When Children Are Not Just “Small Adults”

2008 ◽  
Vol 54 (7) ◽  
pp. 1176-1182 ◽  
Author(s):  
Christina Kanaka-Gantenbein ◽  
Alexandra Margeli ◽  
Panagiota Pervanidou ◽  
Sophia Sakka ◽  
George Mastorakos ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Binding Protein ◽  
Assessment Model ◽  
Retinol Binding Protein ◽  
Morbidly Obese ◽  
Homa Index ◽  
Lipocalin 2 ◽  
Obese Adults ◽  
Retinol Binding Protein 4 ◽  
Hs Crp

Abstract Background: Although there is much evidence regarding the physiologic and pathogenic roles of the newly described adipokines retinol-binding protein 4 (RBP4) and lipocalin-2 as potential promoters of insulin resistance in obese adults, relatively little information exists regarding their roles in obese children. Methods: We investigated the circulating concentrations of RBP4 and lipocalin-2 in 80 obese girls (ages 9– 15 years) and their relationships with high-sensitivity C-reactive protein (hs-CRP) and the adipokines leptin and adiponectin. We divided participants by their body mass index standard deviation scores (BMI SDSs) into 4 groups of 20 girls each: overweight [mean BMI SDS (SD), 1.8 (0.4)], obese [2.2 (0.4)], morbidly obese [3.6 (0.4)], and lean controls [−0.11 (0.4)]. We measured plasma-soluble RBP4, the RBP4-binding protein transthyretin, lipocalin-2, hs-CRP, leptin, and adiponectin and calculated the homeostatic assessment model (HOMA) index from fasting glucose and insulin concentrations. Results: Unexpectedly, plasma RBP4 and lipocalin-2 concentrations were correlated negatively with BMI SDS values (P = 0.005, and P < 0.03, respectively). These results were different from those of adults and were not correlated with the HOMA index. In contrast, hs-CRP and leptin concentrations were positively correlated with BMI SDS values (P < 0.0001, and P < 0.00001, respectively), as expected, whereas the adiponectin concentration was negatively correlated (P = 0.008). Conclusions: Although the correlations of leptin, adiponectin, and hs-CRP concentrations with BMI in children are similar to those of adults, the correlations of RBP4 and lipocalin-2 with BMI in children are the inverse of those observed in adults. Thus, although systemic inflammation and mild insulin resistance are present in childhood obesity, RBP4 and lipocalin-2 concentrations are not increased in children as they are in obese adults with long-standing severe insulin resistance and type 2 diabetes.

scholarly journals Διερεύνηση της σχέσης μεταξύ retinol binding protein 4 και της αντίστασης στην ινσουλίνη στον αιμοκαθαιρόμενο πληθυσμό

2017 ◽  
Author(s):  
Βασίλειος Παπαβασιλείου
Keyword(s):  
Insulin Resistance ◽  
Binding Protein ◽  
Resistance Index ◽  
Retinol Binding Protein ◽  
Model Assessment ◽  
Homeostasis Model Assessment ◽  
Retinol Binding Protein 4 ◽  
Insulin Resistance Index

Εισαγωγή: Η δεσμεύουσα-τη-ρετινόλη πρωτεΐνη 4 (Retinol Binding Protein 4 - RBP-4) είναι η μοναδική, ειδική πρωτεΐνη-μεταφορέας της ρετινόλης (βιταμίνη Α) στο αίμα και ως πρόσφατα η μόνη γνωστή της λειτουργία ήταν η μεταφορά της ρετινόλης στους ιστούς. Νεότερες μελέτες έχουν δείξει ότι η RBP-4 φαίνεται να εμπλέκεται στην εμφάνιση της χρόνιας φλεγμονής καθώς και στην ανάπτυξη της αντίστασης της ινσουλίνης. Η αντίσταση στην ινσουλίνη και η χρόνια φλεγμονή είναι συνήθεις χρόνιες καταστάσεις σε ασθενείς με τελικού σταδίου νεφρική νόσο (ΧΝΝ) που υποβάλλονται σε αιμοκάθαρση. Ωστόσο, δεν είναι γνωστό κατά πόσο τα επίπεδα της RBP-4 είναι αυξημένα στους ασθενείς με ΧΝΝ τελικού σταδίου και εάν σχετίζονται με παράγοντες που εμπλέκονται στην ανάπτυξη της αντίστασης στην ινσουλίνη.Σκοπός: Ο σκοπός λοιπόν της παρούσας μελέτης ήταν να εξετάσει τα επίπεδα της RBP-4 στους αιμοκαθαιρόμενους ασθενείς και να διερευνήσει την πιθανότητα συσχέτισης των επιπέδων της με την αντίσταση στην ινσουλίνη, σε ασθενείς με χρόνια νεφρική ανεπάρκεια τελικού σταδίου υπό αιμοκάθαρση. Μεθοδολογία: Συμμετέχοντες αναζητήθηκαν μεταξύ των ασθενών με ΧΝΝ τελικού σταδίου (ομάδα HD), οι οποίοι βρίσκονταν υπό συστηματική αιμοκάθαρση στη Μονάδα Τεχνητού Νεφρού του Πανεπιστημιακού Νοσοκομείου Λάρισας και “υγιών” εθελοντών από την ευρύτερη περιοχή της Θεσσαλίας. Οι εθελοντές και των 2 ομάδων έπρεπε να πληρούν όλα τα κριτήρια εισαγωγής στη μελέτη. Μετά την έγγραφη συγκατάθεσή τους συμμετείχαν σε μια σειρά από δοκιμασίες και εξετάσεις για την αξιολόγηση της κλινικής τους εικόνας, σωματικής σύστασης, ορμονικού προφίλ, αιματολογικών και βιοχημικών παραμέτρων, των επιπέδων αντίστασης στην ινσουλίνη με τον δείκτη Homeostasis Model Assessment Insulin Resistance Index (HOMA-IR) και των επιπέδων της RBP-4 πρωτεΐνης. Η μελέτη είχε έγκριση από την Επιτροπή Βιοηθικής και Δεοντολογίας του Πανεπιστημιακού Νοσοκομείου Λάρισας και από την Ιατρική Σχολή του Πανεπιστημίου Θεσσαλίας. Αποτελέσματα: Στην παρούσα μελέτη συμμετείχαν 35 ασθενείς με ΧΝΝ τελικού σταδίου (ομάδα HD, 12Α/13Θ, 53,8±18,1 έτη) και 27 υγιείς συμμετέχοντες (ομάδα CON, 16Α/11Θ, 52,1±13,2 έτη) με φυσιολογικές τιμές κρεατινίνης. Τα αποτελέσματα έδειξαν ότι οι αιμοκαθαιρόμενοι ασθενείς έχουν σημαντικά αυξημένα τα επίπεδα της RBP-4 σε σχέση με την ομάδα ελέγχου. Τα επίπεδά της δεν συσχετίστηκαν στατιστικά σημαντικά με τα επίπεδα αντίστασης στην ινσουλίνη αλλά κυρίως με παράγοντες όπως η γλυκόζη νηστείας, η ολική χοληστερόλη, τα επίπεδα σιδήρου καθώς και οι θυρεοειδικές ορμόνες και η παραθορμόνη. Ο δείκτης ΗΟΜΑ-IR βρέθηκε να σχετίζεται με τα σωματομετρικά χαρακτηριστικά των ασθενών. Συμπεράσματα: Τα επίπεδα της RBP-4 είναι αυξημένα στους αιμοκαθαιρόμενους ασθενείς. Φαίνεται δε πως η πρωτεΐνη αυτή εμπλέκεται σε μεταβολικά μονοπάτια χρόνιας φλεγμονής. Δεν βρέθηκε ωστόσο κάποια σημαντική συσχέτιση μεταξύ των επιπέδων της RBP-4 και της αντίστασης στην ινσουλίνη, όπως αυτή αξιολογήθηκε από τον δείκτη ΗΟΜΑ-IR. Συμπερασματικά, τα επίπεδα της RBP-4 θα μπορούσαν να αποτελέσουν ένα σημαντικό δείκτη για την αξιολόγηση του μεταβολικού προφίλ των αιμοκαθαιρόμενων ασθενών και δυνητικά να θεωρηθούν ένα υποστηρικτικό προγνωστικό εργαλείο για την παρακολούθηση της υγείας των ασθενών με χρόνια νεφρική νόσο.

scholarly journals Plasma retinol-binding protein 4 in the first and second trimester and risk of gestational diabetes mellitus in Chinese women: a nested case-control study

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Chuyao Jin ◽  
Lizi Lin ◽  
Na Han ◽  
Zhiling Zhao ◽  
Zheng Liu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Binding Protein ◽  
First Trimester ◽  
Retinol Binding Protein ◽  
Second Trimester ◽  
Retinol Binding Protein 4 ◽  
Plasma Retinol

Abstract Background To assess the association between plasma retinol-binding protein 4 (RBP4) levels both in the first trimester and second trimester and risk of gestational diabetes mellitus (GDM). Methods Plasma RBP4 levels and insulin were measured among 135 GDM cases and 135 controls nested within the Peking University Birth Cohort in Tongzhou. Multivariable linear regression analysis was conducted to assess the influence of RBP4 levels on insulin resistance. Conditional logistic regression models were used to compute the odds ratio (OR) and 95% confidence interval (CI) between RBP4 levels and risk of GDM. Results The GDM cases had significantly higher levels of RBP4 in the first trimester than controls (medians: 18.0 μg/L vs 14.4 μg/L; P < 0.05). Plasma RBP4 concentrations in the first and second trimester were associated with fasting insulin, homeostasis model assessment for insulin resistance (HOMA-IR), and the quantitative insulin sensitivity check index (QUICKI) in the second trimester (all P < 0.001). With adjustment for diet, physical activity, and other risk factors for GDM, the risk of GDM increased with every 1-log μg/L increment of RBP4 levels, and the OR (95% CI) was 3.12 (1.08–9.04) for RBP4 in the first trimester and 3.38 (1.03–11.08) for RBP4 in the second trimester. Conclusions Plasma RBP4 levels both in the first trimester and second trimester were dose-dependently associated with increased risk of GDM.

Association of Retinol-Binding Protein 4 with Arteriovenous Fistula Dysfunction in Hemodialysis Patients

2021 ◽  
pp. 1-8
Author(s):  
Yuanhao Wu ◽  
Fan Wang ◽  
Tingting Wang ◽  
Yin Zheng ◽  
Li You ◽  
...  
Keyword(s):  
Risk Factor ◽  
Arteriovenous Fistula ◽  
Independent Risk Factor ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Youden Index ◽  
Serum Concentrations ◽  
Retinol Binding Protein 4 ◽  
Kaplan Meier ◽  
Increased Risk

<b><i>Background:</i></b> Arteriovenous fistula (AVF) is the most common vascular access for patients undergoing hemodialysis (HD). Neointimal hyperplasia (NIH) might be a potential mechanism of AVF dysfunction. Retinol-binding protein 4 (RBP4) may play an important role in the pathogenesis of NIH. The aim of this study was to investigate whether AVF dysfunction is associated with serum concentrations of RBP4 in HD subjects. <b><i>Methods:</i></b> A cohort of 65 Chinese patients undergoing maintenance HD was recruited between November 2017 and June 2019. The serum concentrations of RBP4 of each patient were measured with the ELISA method. Multivariate logistic regression was used to analyze data on demographics, biochemical parameters, and serum RBP4 level to predict AVF dysfunction events. The cutoff for serum RBP4 level was derived from the highest score obtained on the Youden index. Survival data were analyzed with the Cox proportional hazards regression analysis and Kaplan-Meier method. <b><i>Results:</i></b> Higher serum RBP4 level was observed in patients with AVF dysfunction compared to those without AVF dysfunction events (174.3 vs. 168.4 mg/L, <i>p</i> = 0.001). The prevalence of AVF dysfunction events was greatly higher among the high RBP4 group (37.5 vs. 4.88%, <i>p</i> = 0.001). In univariate analysis, serum RBP4 level was statistically significantly associated with the risk of AVF dysfunction (OR = 1.015, 95% CI 1.002–1.030, <i>p</i> = 0.030). In multivariate analysis, each 1.0 mg/L increase in RBP4 level was associated with a 1.023-fold-increased risk of AVF dysfunction (95% CI for OR: 1.002–1.045; <i>p</i> = 0.032). The Kaplan-Meier survival analysis indicated that the incidence of AVF dysfunction events in the high RBP4 group was significantly higher than that in the low-RBP4 group (<i>p</i> = 0.0007). Multivariate Cox regressions demonstrated that RBP4 was an independent risk factor for AVF dysfunction events in HD patients (HR = 1.015, 95% CI 1.001–1.028, <i>p</i> = 0.033). <b><i>Conclusions:</i></b> HD patients with higher serum RBP4 concentrations had a relevant higher incidence of arteriovenous dysfunction events. Serum RBP4 level was an independent risk factor for AVF dysfunction events in HD patients.

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