scholarly journals Restoration of Iodide Uptake in Dedifferentiated Thyroid Carcinoma: Relationship to Human Na+/I− Symporter Gene Methylation Status1

1999 ◽  
Vol 84 (7) ◽  
pp. 2449-2457 ◽  
Author(s):  
Gopalakrishnan M. Venkataraman ◽  
Mustafa Yatin ◽  
Regina Marcinek ◽  
Kenneth B. Ain
Keyword(s):  
Thyroid Carcinoma ◽  
Gene Methylation ◽  
Iodide Uptake ◽  
Dedifferentiated Thyroid Carcinoma

Reestablishment of In Vitro and In Vivo Iodide Uptake by Transfection of the Human Sodium Iodide Symporter (hNIS) in a hNIS Defective Human Thyroid Carcinoma Cell Line

Thyroid ◽  
2000 ◽  
Vol 10 (11) ◽  
pp. 939-943 ◽  
Author(s):  
Jan W.A. Smit ◽  
Janny P. Schröder-van der Elst ◽  
Marcel Karperien ◽  
Ivo Que ◽  
Gabri van der Pluijm ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Sodium Iodide ◽  
Carcinoma Cell ◽  
Human Thyroid ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake ◽  
Thyroid Carcinoma Cell

Iodide uptake in human anaplastic thyroid carcinoma cells after transfer of the human thyroid peroxidase gene

2001 ◽  
Vol 28 (5) ◽  
pp. 633-638 ◽  
Author(s):  
Uwe Haberkorn ◽  
Annette Altmann ◽  
Shiming Jiang ◽  
Iris Morr ◽  
Miriam Mahmut ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Human Thyroid ◽  
Thyroid Peroxidase ◽  
Iodide Uptake ◽  
Peroxidase Gene ◽  
Human Thyroid Peroxidase

scholarly journals Bexarotene increases uptake of radioiodide in metastases of differentiated thyroid carcinoma

2006 ◽  
Vol 154 (4) ◽  
pp. 525-531 ◽  
Author(s):  
Ying Y Liu ◽  
Marcel P Stokkel ◽  
Alberto M Pereira ◽  
Eleonora P Corssmit ◽  
Hans A Morreau ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Single Photon ◽  
Treatment Options ◽  
Photon Emission ◽  
Ct Scanning ◽  
Differentiated Thyroid Carcinoma ◽  
Whole Body ◽  
Iodide Uptake ◽  
Beneficial Effects

Objective: Treatment options for metastases of differentiated thyroid carcinoma (DTC) are limited due to decreased uptake of radioiodide (I-131). Therefore, strategies to improve I-131 uptake are mandatory. It has been suggested that retinoids have beneficial effects on iodide uptake in vitro and in humans. However, to date, only studies with 13-cis-retinoic acid have been performed in humans. We therefore decided to study the effects of 6 weeks of treatment with the retinoid X receptor activator bexarotene on I-131 uptake in patients with metastatic DTC. Design: Open prospective intervention study. Methods: Twelve patients with metastases of DTC, with insufficient uptake of I-131, received 6 weeks of treatment with 300 mg bexarotene/day. Prior to, and after this intervention, I-131 uptake was measured by whole-body scintigraphy and single photon emission tomography (SPECT) 3 days after 185 MBq I-131. Diagnostic imaging was preceded by two consecutive injections of recombinant human TSH. Results: Bexarotene treatment induced I-131 uptake in metastases of 8 out of 11 patients (one patient died for reasons not related to the study). However, uptake was only discernable at SPECT and had incomplete matching with metastases as visualized by CT scanning. Conclusions: Bexarotene partially restores I-131 uptake in metastases of DTC. The clinical relevance of this observation may be limited due to the differential responses of the different metastases within each patient and the low intensity of I-131 uptake.

scholarly journals NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259558
Author(s):  
Yuko Ito ◽  
Fumihiko Furuya ◽  
Katsumi Taki ◽  
Hideaki Suzuki ◽  
Hiroki Shimura
Keyword(s):  
Cell Death ◽  
Thyroid Cancer ◽  
Thyroid Carcinoma ◽  
Protein Signaling ◽  
Cancer Cell Death ◽  
Thyroid Transcription Factor ◽  
Dedifferentiated Thyroid Carcinoma ◽  
Cancer Phenotypes ◽  
Underlying Mechanisms ◽  
Apoptosis And Necrosis

NK2 homeobox 1 (NKX2-1) is a thyroid transcription factor essential for proper thyroid formation and maintaining its physiological function. In thyroid cancer, NKX2-1 expression decreases in parallel with declined differentiation. However, the molecular pathways and mechanisms connecting NKX2-1 to thyroid cancer phenotypes are largely unknown. This study aimed to examine the effects of NKX2-1 re-expression on dedifferentiated thyroid cancer cell death and explore the underlying mechanisms. A human papillary thyroid carcinoma cell line lacking NKX2-1 expression was infected with an adenoviral vector containing Nkx2-1. Cell viability decreased after Nkx2-1 transduction and apoptosis and necrosis were detected. Arginase 2 (ARG2), regulator of G protein signaling 4 (RGS4), and RGS5 mRNA expression was greatly increased in Nkx2-1-transducted cells. After suppressing these genes by siRNA, cell death, apoptosis, and necrosis decreased in RGS4 knockdown cells. These findings demonstrated that cell death was induced via apoptosis and necrosis by NKX2-1 re-expression and involves RGS4.

scholarly journals PLEKHS1 Over-Expression is Associated with Metastases and Poor Outcomes in Papillary Thyroid Carcinoma

Cancers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2133
Author(s):  
Xiangling Xing ◽  
Ninni Mu ◽  
Xiaotian Yuan ◽  
Na Wang ◽  
C. Christofer Juhlin ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Disease Free Survival ◽  
Distant Metastases ◽  
Pleckstrin Homology Domain ◽  
Gene Methylation ◽  
Akt Phosphorylation ◽  
Over Expression ◽  
Tert Promoter Mutations ◽  
Poor Outcomes ◽  
Promoter Mutations

Pleckstrin homology domain containing S1 (PLEKHS1) is a poorly characterized factor, although its promoter mutations were identified in human malignancies including thyroid carcinoma (TC). This study was designed to determine PLEKHS1 promoter hotspot mutations in papillary and anaplastic thyroid carcinomas (PTCs and ATCs) and to evaluate if PLEKHS1 expression influences clinical outcome. The PLEKHS1 promoter mutation was observed in 1/93 of PTCs and none of 18 ATCs in our cohort; however, PLEKHS1 expression was aberrantly up-regulated in TCs compared to adjacent non-tumorous thyroid tissues. ATC tumors, an undifferentiated TC, exhibited the highest PLEKHS1 expression. In both TCGA and present cohorts of PTCs, PLEKHS1 gene methylation density was inversely correlated with its mRNA expression and demethylation at the PLEKHS1 locus occurred at two CpGs. Higher PLEKHS1 expression was associated with lymph node and distant metastases, and shorter overall and disease-free survival in our cohort of PTC patients. Importantly, PLEKHS1 over-expression predicted shorter patient survival in PTCs lacking TERT promoter mutations. Cellular experiments showed that PLEKHS1 over-expression enhanced AKT phosphorylation and invasiveness. Collectively, the PLEKHS1 gene demethylation causes its over-expression in PTCs. PLEKHS1 promotes aggressive behavior of TCs possibly by increasing AKT activity, and its over-expression predicts poor patient outcomes.

scholarly journals Differential effects of natural flavonoids on growth and iodide content in a human Na*/I- symporter-transfected follicular thyroid carcinoma cell line

2004 ◽  
pp. 557-564 ◽  
Author(s):  
JP Schroder-van der Elst ◽  
D van der Heide ◽  
JA Romijn ◽  
JW Smit
Keyword(s):  
Thyroid Cancer ◽  
Thyroid Carcinoma ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Thyroid Peroxidase ◽  
Thyroid Cancer Cell ◽  
Iodide Uptake ◽  
Iodide Transport

OBJECTIVE: Natural flavonoids (plant pigments) have been shown to inhibit thyroid peroxidase (TPO) in vitro and the growth of thyroid cancer cell lines. We have studied the role of flavonoids on the iodide transport and the growth of the human follicular thyroid cancer cell line (FTC133) which was stably transfected with the human Na(+)/I(-) symporter (hNIS). DESIGN AND METHODS: Cells were treated with flavonoids (0.5-50 microM) for 0, 2, 4 and 6 days; (125)I content and (125)I efflux of the cells and DNA content were measured. RESULTS: Cell growth was inhibited significantly at day 6 by most flavonoids. Eight out of ten flavonoids decreased the (125)I content of the cells at day 4. Morin did not influence the (125)I content of the cells and, surprisingly, myricetin increased the (125)I content of the cells. Kaempferol, apigenin, luteolin and F21388 decreased NIS mRNA expression after 15, 29 and 48 h; after 96 h NIS mRNA returned to normal. CONCLUSION: As TPO is not present in this cell line, the effects of the flavonoids on the iodide uptake are not related to organification. Myricetin was the only flavonoid studied that increased the influx and decreased the efflux of iodide. The effect of myricetin (decreased growth and increased retention of iodide) can be of therapeutic value in the radioiodide treatment of thyroid carcinoma.

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