ERβ1 and the ERβ2 Splice Variant (ERβcx/β2) Are Expressed in Distinct Cell Populations in the Adult Human Testis

Identification of genes specifically expressed in adult and fetal testis is important in furthering our understanding of testis development and function. In this study, a novel human transcript, designated human testis cAMP-responsive element-binding protein (htCREB), was identified by hybridization of adult and fetal human testis cDNA probes with a human cDNA microarray containing 9216 clones. The htCREB transcript (GenBank Accession no. AY347527) was expressed at 2.35-fold higher levels in adult human testes than in fetal testes. Sequence and ntBLAST analyses against the human genome database indicated that htCREB was a novel splice variant of human CREB. RT-PCR-based tissue distribution experiments demonstrated that the htCREB transcript was highly expressed in adult human testis and in healthy sperm, but not in testes from patients with Sertoli cell-only syndrome. Taken together, these results suggest that the htCREB transcript is chiefly expressed in germ cells and is most likely involved in spermatogenesis.

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Faculty Opinions recommendation of Generation of pluripotent stem cells from adult human testis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1124139.598955 ◽

2009 ◽

Author(s):

Paul Turek

Keyword(s):

Stem Cells ◽

Pluripotent Stem Cells ◽

Human Testis ◽

Adult Human

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Faculty Opinions recommendation of Generation of pluripotent stem cells from adult human testis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1124139.581273 ◽

2008 ◽

Author(s):

Takashi Minegishi

Keyword(s):

Stem Cells ◽

Pluripotent Stem Cells ◽

Human Testis ◽

Adult Human

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Expression of a novel HsMCAK mRNA splice variant, tsMCAK gene, in human testis

Life Sciences ◽

10.1016/s0024-3205(02)02079-9 ◽

2002 ◽

Vol 71 (23) ◽

pp. 2741-2757 ◽

Cited By ~ 11

Author(s):

Li Jun Cheng ◽

Zuo Min Zhou ◽

Jian Min Li ◽

Hui Zhu ◽

Hu Zhu ◽

...

Keyword(s):

Splice Variant ◽

Human Testis ◽

Mrna Splice

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Stage-specific Localization and Expression of c-kit in the Adult Human Testis

Journal of Histochemistry & Cytochemistry ◽

10.1369/jhc.2009.953737 ◽

2009 ◽

Vol 57 (9) ◽

pp. 861-869 ◽

Cited By ~ 41

Author(s):

Sreepoorna K. Unni ◽

Deepak N. Modi ◽

Shilpa G. Pathak ◽

Jayesh V. Dhabalia ◽

Deepa Bhartiya

Keyword(s):

Current Knowledge ◽

Protein Localization ◽

Immunohistochemical Analysis ◽

Human Testis ◽

Specific Expression ◽

Adult Human ◽

Kit Receptor ◽

Specific Localization ◽

Human Spermatogenesis ◽

And Function

The c-kit receptor (KIT) and its ligand, stem cell factor (SCF), represent one of the key regulators of testicular formation, development, and function and have been extensively studied in various animal models. The present study was undertaken to characterize the pattern of localization and expression of c-kit in normal adult human testis. Immunohistochemical analysis showed that KIT is expressed in the cytoplasm of spermatogonia, acrosomal granules of spermatids, and Leydig cells. Interestingly, a rather heterogenous pattern of expression of the protein along the basement membrane was observed. Intense protein localization in spermatogonia was detected in stages I–III, whereas low expression was observed in stages IV–VI of the seminiferous epithelium, indicating that the expression of the molecule was stage specific. In situ hybridization studies revealed that the transcripts of the gene were also localized in a similar non-uniform pattern. To the best of our knowledge, such a stage-specific expression of KIT has not been reported previously in the human testis. The results of the present study may expand current knowledge about the c-kit/SCF system in human spermatogenesis.

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Chemogenetic Tools for Causal Cellular and Neuronal Biology

Physiological Reviews ◽

10.1152/physrev.00009.2017 ◽

2018 ◽

Vol 98 (1) ◽

pp. 391-418 ◽

Cited By ~ 23

Author(s):

Deniz Atasoy ◽

Scott M. Sternson

Keyword(s):

G Protein ◽

Ex Vivo ◽

Cell Types ◽

Cell Populations ◽

Specific Cell ◽

Cellular Processes ◽

Distinct Cell ◽

G Protein Coupled ◽

Pharmacological Control

Chemogenetic technologies enable selective pharmacological control of specific cell populations. An increasing number of approaches have been developed that modulate different signaling pathways. Selective pharmacological control over G protein-coupled receptor signaling, ion channel conductances, protein association, protein stability, and small molecule targeting allows modulation of cellular processes in distinct cell types. Here, we review these chemogenetic technologies and instances of their applications in complex tissues in vivo and ex vivo.

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Revival of Calcium-Binding Proteins for Neuromorphology: Secretagogin Typifies Distinct Cell Populations in the Avian Brain

Brain Behavior and Evolution ◽

10.1159/000357834 ◽

2014 ◽

Vol 83 (2) ◽

pp. 82-92 ◽

Cited By ~ 9

Author(s):

Georgina Gáti ◽

Dávid Lendvai ◽

Tomas Hökfelt ◽

Tibor Harkany ◽

Alán Alpár

Keyword(s):

Calcium Binding ◽

Binding Proteins ◽

Calcium Binding Proteins ◽

Cell Populations ◽

Avian Brain ◽

Distinct Cell

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Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late blastopore lip

Development ◽

10.1242/dev.119.4.991 ◽

1993 ◽

Vol 119 (4) ◽

pp. 991-1004 ◽

Cited By ~ 32

Author(s):

L.K. Gont ◽

H. Steinbeisser ◽

B. Blumberg ◽

E.M. de Robertis

Keyword(s):

Cell Populations ◽

Fate Map ◽

Stages Of Development ◽

Gene Markers ◽

Distinct Cell ◽

Multiple Cell ◽

Organizer Activity ◽

Dorsal Cells ◽

Late Stages ◽

Lines Of Evidence

Three lines of evidence suggest that tail formation in Xenopus is a direct continuation of events initiated during gastrulation. First, the expression of two gene markers, Xbra and Xnot2, can be followed from the blastopore lip into distinct cell populations of the developing tailbud. Second, the tip of the tail retains Spemann's tail organizer activity until late stages of development. Third, lineage studies with the tracer DiI indicate that the cells of the late blastopore are fated to form specific tissues of the tailbud, and that intercalation of dorsal cells continues during tail elongation. In particular, the fate map shows that the tip of the tail is a direct descendant of the late dorsal blastopore lip. Thus, the tailbud is not an undifferentiated blastema as previously thought, but rather consists of distinct cell populations which arise during gastrulation.

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