Myoblast migration specifically inhibited in the chick embryo by grafted CSAT hybridoma cells secreting an anti-integrin antibody

Development ◽  
1988 ◽  
Vol 103 (3) ◽  
pp. 431-446 ◽  
Author(s):  
T. Jaffredo ◽  
A.F. Horwitz ◽  
C.A. Buck ◽  
P.M. Rong ◽  
F. Dieterlen-Lievre
Keyword(s):  
Chick Embryo ◽  
Body Wall ◽  
Developmental Process ◽  
Hybridoma Cells ◽  
General Interest ◽  
Abdominal Muscles ◽  
Muscle Formation ◽  
Body Wall Muscles ◽  
Long Term Consequences

We report a teratological method in which mouse hybridoma cells are grafted into a chick host. CSAT (Cell Substratum ATtachment) hybridoma was used. It produces an antibody directed against the avian integrin complex. The grafts were performed during the second and third days of incubation either at the level of the somites or in the coelom of the chick embryo. The anomalies were revealed by means of a monoclonal antibody that recognizes myogenic cells as soon as they become committed in the myotome. When embryos were grafted at the level of the somites, body wall muscles failed to develop on the side of the graft only. After coelomic grafting, total agenesis of abdominal muscles was induced. The anomalies were specific since the engraftment of three control hybridoma clones induced no change in muscle formation. These control hybridomas produce antibodies directed against the same molecular complex but not against the same epitope as CSAT. The injection of hybridoma cells in an embryo appears as a method of general interest to determine the long-term consequences of perturbing a specific developmental process.

scholarly journals Hypoxia Induces Dilated Cardiomyopathy in the Chick Embryo: Mechanism, Intervention, and Long-Term Consequences

PLoS ONE ◽  
2009 ◽  
Vol 4 (4) ◽  
pp. e5155 ◽  
Author(s):  
Andrei Tintu ◽  
Ellen Rouwet ◽  
Stefan Verlohren ◽  
Joep Brinkmann ◽  
Shakil Ahmad ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Dilated Cardiomyopathy ◽  
Long Term Consequences

Calsequestrin, a calcium sequestering protein localized at the sarcoplasmic reticulum, is not essential for body-wall muscle function in Caenorhabditis elegans

2000 ◽  
Vol 113 (22) ◽  
pp. 3947-3958 ◽  
Author(s):  
J.H. Cho ◽  
Y.S. Oh ◽  
K.W. Park ◽  
J. Yu ◽  
K.Y. Choi ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Binding ◽  
Body Wall ◽  
Functional Characterization ◽  
The Body ◽  
Body Wall Muscle ◽  
C Elegans ◽  
Muscle Formation ◽  
Body Wall Muscles

Calsequestrin is the major calcium-binding protein of cardiac and skeletal muscles whose function is to sequester Ca(2+)in the lumen of the sarcoplasmic reticulum (SR). Here we describe the identification and functional characterization of a C. elegans calsequestrin gene (csq-1). CSQ-1 shows moderate similarity (50% similarity, 30% identity) to rabbit skeletal calsequestrin. Unlike mammals, which have two different genes encoding cardiac and fast-twitch skeletal muscle isoforms, csq-1 is the only calsequestrin gene in the C. elegans genome. We show that csq-1 is highly expressed in the body-wall muscles, beginning in mid-embryogenesis and maintained through the adult stage. In body-wall muscle cells, CSQ-1 is localized to sarcoplasmic membranes surrounding sarcomeric structures, in the regions where ryanodine receptors (UNC-68) are located. Mutation in UNC-68 affects CSQ-1 localization, suggesting that the two possibly interact in vivo. Genetic analyses of chromosomal deficiency mutants deleting csq-1 show that CSQ-1 is not essential for initiation of embryonic muscle formation and contraction. Furthermore, double-stranded RNA injection resulted in animals completely lacking CSQ-1 in body-wall muscles with no observable defects in locomotion. These findings suggest that although CSQ-1 is one of the major calcium-binding proteins in the body-wall muscles of C. elegans, it is not essential for body-wall muscle formation and contraction.

Barriers to Physical Activity in Adolescents

2015 ◽  
Vol 23 (2) ◽  
pp. 47-59 ◽  
Author(s):  
Nadine Langguth ◽  
Tanja Könen ◽  
Simone Matulis ◽  
Regina Steil ◽  
Caterina Gawrilow ◽  
...  
Keyword(s):  
Physical Activity ◽  
Leisure Activities ◽  
Outcome Expectations ◽  
Multidimensional Approach ◽  
Factor Analyses ◽  
Physical And Mental Health ◽  
Tailored Interventions ◽  
Long Term Consequences ◽  
Age Range

During adolescence, physical activity (PA) decreases with potentially serious, long-term consequences for physical and mental health. Although barriers have been identified as an important PA correlate in adults, research on adolescents’ PA barriers is lacking. Thus reliable, valid scales to measure adolescents’ PA barriers are needed. We present two studies describing a broad range of PA barriers relevant to adolescents with a multidimensional approach. In Study 1, 124 adolescents (age range = 12 – 24 years) reported their most important PA barriers. Two independent coders categorized those barriers. The most frequent PA barriers were incorporated in a multidimensional questionnaire. In Study 2, 598 adolescents (age range = 13 – 21 years) completed this questionnaire and reported their current PA, intention, self-efficacy, and negative outcome expectations. Seven PA barrier dimensions (leisure activities, lack of motivation, screen-based sedentary behavior, depressed mood, physical health, school workload, and preconditions) were confirmed in factor analyses. A multidimensional approach to measuring PA barriers in adolescents is reliable and valid. The current studies provide the basis for developing individually tailored interventions to increase PA in adolescents.

Short- and Long-Term Consequences of Directed Forgetting

2012 ◽  
Author(s):  
Sara B. Festini ◽  
Patricia A. Reuter-Lorenz
Keyword(s):  
Directed Forgetting ◽  
Short And Long Term ◽  
Long Term Consequences

Long-Term Consequences of Volunteering: Undergraduate Community Service Touches the Future

2008 ◽  
Author(s):  
Judy Primavera ◽  
Andrew Martinez
Keyword(s):  
Community Service ◽  
The Future ◽  
Long Term Consequences

Responding to Immediate and Long-Term Consequences of Terror

2008 ◽  
Author(s):  
Mary Fabri ◽  
Amber Gray ◽  
Jeannette Uwineza
Keyword(s):  
Long Term Consequences

Themes, Diction, and Prosodic Systems in Yoruba Lullabies

2019 ◽  
Vol 12 (1) ◽  
pp. 18-33 ◽  
Author(s):  
Olusanmi Babarinde ◽  
Elizabeth Babarinde
Keyword(s):  
Participant Observation ◽  
Cultural Beliefs ◽  
Survey Method ◽  
World Views ◽  
Scholarly Research ◽  
Long Term Consequences ◽  
Analyse Data ◽  
Descriptive Survey ◽  
Future Learning

Lullabies are essentially sung for their soothing nature but, as this article shows, they have other important functions. One of the most important of these is that lullabies may provide much-needed language stimulation with important long-term consequences for future learning. This paper begins the work of addressing the dearth of scholarly research on lullabies, especially in the Yoruba (Nigeria: Niger-Congo) culture. It looks at the range of themes, dictions, and prosody that are intertwined to reveal Yoruba beliefs and world-views about children, starting with their time in the womb. The study uses a descriptive survey method to analyse data collected through participant observation. It shows that Yoruba lullabies not only offer insights into Yoruba cultural beliefs but also depend greatly on figurative expression and prosodic systems. These rich literary qualities identify lullabies as the earliest sub-genre of children's poetry.

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