Measurements of cell adhesion

Development ◽  
1973 ◽  
Vol 30 (2) ◽  
pp. 511-518
Author(s):  
Janet E. Hornby
Keyword(s):  
Cell Adhesion ◽  
Calcium Ions ◽  
Limb Bud ◽  
Magnesium Ions ◽  
Divalent Ions

The forces of interaction found for 5-day chick limb-bud cells in media containing different divalent ions (magnesium, calcium, strontium or barium) show that the cells are most adhesive in the presence of magnesium ions, then calcium and strontium, then barium. When magnesium and calcium ions are present together at similar concentrations the calcium ions modify the action of the magnesium ions. The importance of magnesium ions in cell adhesion is discussed.

THE METABOLISM OF THE ERYTHROCYTE: X. THE INORGANIC PYROPHOSPHATASE OF THE ERYTHROCYTE

1956 ◽  
Vol 34 (1) ◽  
pp. 121-129 ◽  
Author(s):  
A. Malkin ◽  
O. F. Denstedt
Keyword(s):  
Enzyme Activity ◽  
Calcium Ions ◽  
Human Erythrocyte ◽  
Inorganic Pyrophosphatase ◽  
Magnesium Ions ◽  
Constant Ratio ◽  
Inorganic Pyrophosphate ◽  
Noncompetitive Inhibitor ◽  
Pyrophosphatase Activity ◽  
Hydrolysis Of

The activity of the pyrophosphatase which catalyzes the hydrolysis of inorganic pyrophosphate in the erythrocyte of the human, the rabbit, and the chicken is confined entirely to the cytoplasm of the cell. Following preincubation, the enzyme activity in the human erythrocyte is diminished, but pre-incubation in the presence of cysteine or glutathione prevents the diminution of the enzyme activity. Aging of the hemolyzate of the human erythrocytes results in a marked loss of the inorganic pyrophosphatase activity. The diminished activity can be restored by the addition of cysteine or glutathione to the reaction mixture; but after the hemolyzate has aged for five or six days at 5 °C, the loss in the enzyme activity can no longer be restored with these reagents. Fluoride and calcium ions inhibit the activity of the enzyme, while magnesium ions are essential for its activity. Calcium is a noncompetitive inhibitor, while the inhibition by fluoride is of a "quadratic" nature. If a constant ratio of magnesium to pyrophosphate is maintained, the quadratic inhibition can be converted to the "uncompetitive" type of inhibition.

scholarly journals EFFECTS OF CALCIUM IONS AND MAGNESIUM IONS ON 5-HYDROXYTRYPTAMINE-RECEPTOR INTERACTION

1976 ◽  
Vol 26 (3) ◽  
pp. 382-384 ◽  
Author(s):  
Issei TAKAYANAGI ◽  
Chung Shin LIAO ◽  
Keijiro TAKAGI
Keyword(s):  
Calcium Ions ◽  
Receptor Interaction ◽  
Magnesium Ions

Study of the influence between magnesium ions and calcium ions on the morphology and size of coprecipitation in microemulsion

2014 ◽  
Vol 9 (5) ◽  
pp. 302-307 ◽  
Author(s):  
Nong Wang ◽  
Lijuan Yang ◽  
Lixuan Chen ◽  
Rong Xiao
Keyword(s):  
Calcium Ions ◽  
Magnesium Ions

scholarly journals A STUDY ON THE MECHANISM OF INTERCELLULAR ADHESION

1974 ◽  
Vol 60 (3) ◽  
pp. 641-652 ◽  
Author(s):  
Joris J. Deman ◽  
Erik A. Bruyneel ◽  
Marc M. Mareel
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Calcium Ions ◽  
Hela Cells ◽  
Previous Treatment ◽  
Cell Aggregation ◽  
Sialic Acids ◽  
Magnesium Ions ◽  
Trypsin Treatment ◽  
Calcium Effect

Aggregation of suspended HeLa cells is increased on removal of cell surface sialic acid. Calcium ions promote aggregation whereas magnesium ions have no effect. The calcium effect is abolished by previous treatment of the cells with neuraminidase. Trypsinization of the HeLa cells followed by thorough washing diminishes the rate of mutual cell aggregation. Subsequent incubation with neuraminidase restores the aggregation rate to the original value before trypsin treatment. Cells which had acquired a greater tendency for aggregation after removal of peripheral sialic acid lose this property when subsequently treated with trypsin. Calcium ions have no aggregative effect on trypsinized cells. In contrast to HeLa cells, aggregation of human erythrocytes was not increased after treatment with neuraminidase or on addition of calcium. The results with HeLa cells are interpreted as follows: (a) Trypsin-releasable material confers adhesiveness upon the cells. (b) The adhesive property of this material is counteracted by the presence of cell surface sialic acids. (c) Calcium ions exert their effect by attenuating the adverse effect of sialic acid.

The Effects of Prolactin and Divalent Ions on the Permeability to Water of Fundulus Kansae

1970 ◽  
Vol 53 (2) ◽  
pp. 317-327 ◽  
Author(s):  
W. T. W. POTTS ◽  
W. R. FLEMING
Keyword(s):  
Fresh Water ◽  
Calcium Ions ◽  
Sea Water ◽  
Tritiated Water ◽  
Low Doses ◽  
Divalent Ions ◽  
Water Turnover ◽  
Drinking Rate ◽  
Low Concentration ◽  
Ovine Prolactin

1. Measurements have been made of the rate of exchange of tritiated water in both intact and hypophysectomized Fundulus kansae in a variety of media. 2. Hypophysectomy reduces the rate of exchange in fresh water. 3. Low doses (30 mu) of ovine prolactin stimulate water turnover in hypophysectomized fish in fresh water. 4. The rate of exchange declines in both intact and hypophysectomized animals with increasing salinity. 5. Experiments with synthetic solutions show that the decline in the rate of exchange in sea water and in higher salinities is due mainly to the effects of calcium ions. 6. Fishes maintained in synthetic sea water containing a low concentration of calcium have both a higher rate of exchange of tritiated water and a higher drinking rate than fish in normal sea water.

scholarly journals Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals

2013 ◽  
Vol 79 (9) ◽  
pp. 3085-3090 ◽  
Author(s):  
Rie Murai ◽  
Naoto Yoshida
Keyword(s):  
Calcium Ions ◽  
Microscopic Observation ◽  
Nutrient Medium ◽  
Brilliant Green ◽  
Magnesium Ions ◽  
Vegetative Cells ◽  
Content Type ◽  
Geobacillus Thermoglucosidasius ◽  
Parent Colony

ABSTRACTGeobacillus thermoglucosidasiuscolonies were placed on an agar hydrogel containing acetate, calcium ions, and magnesium ions, resulting in the formation of single calcite crystals (calcites) within and peripheral to the plating area or parent colony. Microscopic observation of purified calcites placed on the surface of soybean casein digest (SCD) nutrient medium revealed interior crevices from which bacterial colonies originated. Calcites formed on the gel contained [1-13C]- and [2-13C]acetate, demonstrating thatG. thermoglucosidasiusutilizes carbon derived from acetate for calcite formation. During calcite formation, vegetative cells swam away from the parent colony in the hydrogel. Hard-agar hydrogel inhibited the formation of calcites peripheral to the parent colony. The calcite dissolved completely in 1 M HCl, with production of bubbles, and the remaining endospore-like particles were easily stained with Brilliant green dye. The presence of DNA and protein in calcites was demonstrated by electrophoresis. We propose that endospores initiate the nucleation of calcites. Endospores ofG. thermoglucosidasiusremain alive and encapsulated in calcites.

scholarly journals Plexin: A novel neuronal cell surface molecule that mediates cell adhesion via a homophilic binding mechanism in the presence of calcium ions

Neuron ◽  
1995 ◽  
Vol 14 (6) ◽  
pp. 1189-1199 ◽  
Author(s):  
Kunimasa Ohta ◽  
Akihito Mizutani ◽  
Atsushi Kawakami ◽  
Yasunori Murakami ◽  
Yasuyo Kasuya ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Calcium Ions ◽  
Neuronal Cell ◽  
Surface Molecule ◽  
Binding Mechanism ◽  
Cell Surface Molecule ◽  
Homophilic Binding

scholarly journals Molecular Properties of Ca2+ Transport Through TRPV2 Channel: A Molecular Dynamic Simulations Study

2021 ◽  
Author(s):  
Mozhgan Alipour ◽  
Behnam Hajipour-Verdom ◽  
Parviz Abdolmaleki ◽  
Mohammad Javan
Keyword(s):  
Calcium Ions ◽  
Electrostatic Interactions ◽  
Potential Of Mean Force ◽  
Divalent Ions ◽  
Energy Fluctuation ◽  
Dynamic Simulations ◽  
Nonselective Cation Channels ◽  
Trpv Channels ◽  
Calculation Results ◽  
Smd Simulations

Abstract TRPV channels are a category of nonselective cation channels that activated by heat and ligands, and permeate monovalent and divalent ions. The mechanism of Ca2+ transfer through TRPV2 channel is not well known. Here, we investigated the reaction coordination and energy fluctuation of Ca2+ transition in TRPV2 channel by steered molecular dynamics (SMD) simulations and potential of mean force (PMF) calculation. Results showed that electrostatic interactions between Ca2+ and residues of the first and second gates had main roles in ions transfer through the channel, and also, we recognized important amino acids in this path. Moreover, results indicated that enter and exit of calcium ions needed to overcome barrier energies in first and second gates.

Sign in / Sign up

Export Citation Format

Share Document