Studies on the development of F1 embryos from inter-strain crosses involving DDK mice

Development ◽  
1977 ◽  
Vol 38 (1) ◽  
pp. 211-216
Author(s):  
Noboru Wakasugi ◽  
Mitsuyo Morita
Keyword(s):  
Blastocyst Stage ◽  
Small Cell ◽  
Mouse Embryos ◽  
Cleavage Rate ◽  
Cytoplasmic Factor ◽  
Cell Numbers ◽  
Significant Difference ◽  
The Mean ◽  
Early Mouse ◽  
Number Of Cells

The development of the early mouse embryos was investigated from 36 to 84 h after the presumed time of fertilization. The mean number of cells constituting the embryo from the intra-strain crosses of three strains at 84 h of development was as follows: DDK, 22·7; BS, 43·1 and 1TES, 62·0. A significant difference was observed in the cleavage rate of the embryo between DDK and the other two strains. The F1 embryos from the crosses of BS females × DDK males and ITES females × DDK males showed almost the same progress of development as BS and ITES embryos, respectively. Therefore, it is concluded that the early development of the F1 embryos is regulated mainly by the factors from the mother. The morulae from the semi-sterile cross, DDK females × BS males, showed conspicuously small cell numbers as compared with their litter-mates that developed to the blastocyst stage. It is inferred that the discrimination is already made during cleavage between the fortunate survivors and the lethal embryos due to the incompatibility between the cytoplasmic factor of DDK eggs and alien spermatozoa.

Lethality of a tritiated amino acid in early mouse embryos

Development ◽  
1985 ◽  
Vol 88 (1) ◽  
pp. 209-217
Author(s):  
Janet L. Wiebold ◽  
Gary B. Anderson
Keyword(s):  
Specific Activity ◽  
Subsequent Development ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Radiation Induced ◽  
Specific Activities ◽  
Tritiated Amino Acids ◽  
Early Mouse

2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. 2- to 4-cell embryos that incorporated an average of 42 d.p.m. per embryo were impaired in their ability to develop to the morula and blastocyst stage. Recipients receiving morulae and blastocysts that had incorporated an average of 384 d.p.m. per embryo failed to produce young. Reduction of the specific activity improved the viability of embryos both in vitro and in vivo but development was still less than that of unlabelled embryos. Protein degradation curves were different for both 2- to 4-cell and morulato blastocyst-stage embryos labelled at the two different specific activities. Most studies using tritiated amino acids have employed higher specific activities than those used here and they may have to be reevaluated due to the possibility of radiation-induced artifacts.

Localization of cytoskeletal proteins in preimplantation mouse embryos

Development ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 211-225
Author(s):  
E. Lehtonen ◽  
R. A. Badley
Keyword(s):  
Blastocyst Stage ◽  
Cytoskeletal Proteins ◽  
Mouse Embryos ◽  
Trophectoderm Cells ◽  
Apparent Concentration ◽  
Preimplantation Mouse ◽  
Early Mouse ◽  
Filament Protein

The immunofluorescence technique was used to detect the presence and distribution of actin, alpha-actinin, tubulin and 10 nm filament protein in early mouse embryos. Actin and alpha-actinin stainings showed a distinct concentration to a peripheral layer in the cleavage-stage blastomeres and in trophectoderm cells. Dots of fluorescence appeared in this cortical staining pattern. The distribution of tubulin staining in the blastomere cytoplasm was relatively even with apparent concentration at the perinuclear region and frequently at wide intercellular contact areas. 10 nm filament protein was distributed evenly in the blastomere cytoplasm without cortical concentration of the label. At the blastocyst stage, the trophectoderm cells in blastocyst outgrowths in vitro developed well organized cytoskeletons including both microfilament, microtubule and 10 nm filament elements. Comparable structures were not observed in blastocysts in vivo, or in late hatched blastocysts cultured in suspension. The morphogenetic significance of the observations is discussed.

277 FOLLICULAR GROWTH, SUBSEQUENT OVUM PICKUP, AND DOMINANT FOLLICLE REMOVAL IN COWS

2006 ◽  
Vol 18 (2) ◽  
pp. 246
Author(s):  
K. Imai ◽  
M. Tagawa ◽  
S. Matoba ◽  
M. Narita ◽  
K. Kanayama
Keyword(s):  
Formation Rate ◽  
Cumulus Cell ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Dominant Follicle ◽  
Cleavage Rate ◽  
Significant Difference ◽  
The Mean ◽  
Follicle Aspiration ◽  
Removal Group

The present study was designed to assess the recruitment of follicles after ovum pickup (OPU) and dominant follicle (DF) removal on the follicular wave after OPU in Holstein dry cows. Cows were reared under the same feeding and environmental conditions. In Experiment 1, follicle aspiration (>2 mm in diameter) by OPU using a 7.5-MHz linear transducer with needle (COVA needle; Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200; ALOKA, Tokyo, Japan) was performed in four cows. Then, ovaries were observed after OPU from Day 1 (Day 0 = the day of OPU) to Day 11 to assess the number of follicles developed. In Experiment 2, two sessions of OPU were performed with a 7 day interval between sessions, with or without dominant follicle removal, to assess the quality of developing follicles and oocytes. In the DF removal group, >8-mm follicles were aspirated at Day 5 after the first OPU session, and the same cows without DF removal were designated as a control (n = 4, crossover trial). Oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. To assess the developmental competence of oocytes, Grades 1 and 2 cumulus-oocyte complexes (COCs) were collected, matured, fertilized, and cultured as described by Imai et al. (2002 J. Vet. Med. Sci. 64(10), 887-891). Embryo development was assessed by the cleavage rate on Day 2 and the blastocyst formation rate on Days 7 to 9 (the day of insemination = Day 0). Data were analyzed by ANOVA or Student t-test. In Experiment 1, a dominant follicle (>8 mm in diameter) was developed during Days 3 to 5 after OPU in each donor. The mean number of developing follicles (>2 mm in diameter) were increased from Day 1 to Day 9 (Day 1: 7.5 � 2.1, Day 3: 19.0 � 1.2, Day 5: 23.3 � 9.0, Day 7: 30.3 � 11.0, Day 9: 42.0 � 15.8 and Day 11: 41.0 � 16.7 (mean � SD), P < 0.05). In Experiment 2, there was no difference in the mean number of developing follicles on the day of OPU and collected oocytes between DF removal and control groups (follicles: 47.8 � 23.0 and 39.3 � 6.2; oocytes: 27.0 � 11.6 and 26.5 � 5.4, respectively). The number of Grades 1 and 2 oocytes for the DF removal group was significantly higher (P < 0.05) than that for the control (83.6 � 1.5 and 63.2 � 14.2, respectively), and no significant difference was found within cleavage (60.0 � 37.2, 53.6 � 23.2) and blastocyst rates (34.1 � 33.9, 34.4 � 16.8). These results indicate that populations of follicles were increased till Day 9 after OPU, and the DF removal was effective at increasing oocyte quality in the developing follicles.

158 IMPACT OF MATURED CATTLE OOCYTES AT HIGHER INCUBATION TEMPERATURE ON IN VITRO EMBRYO PRODUCTION

2016 ◽  
Vol 28 (2) ◽  
pp. 209
Author(s):  
M. Nkadimeng ◽  
E. van Marle-Koster ◽  
K. P. M. Lekola ◽  
M. L. Mphaphathi ◽  
M. M. Seshoka ◽  
...  
Keyword(s):  
Incubation Temperature ◽  
Cell Types ◽  
Cell Number ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Developmental Potential ◽  
Cell Numbers ◽  
Significant Difference ◽  
In Vitro Embryo Production

Heat stress during IVF is associated with reduced fertility in cattle oocytes. It may, however, enhance thermo-tolerance or cause detrimental effects on a variety of cell types or organisms, depending on the duration and intensity of the thermal challenge. The aim of this study was to evaluate the developmental potential of cumulus-oocyte complexes (COC) matured for 18 or 24 h and incubated at 39°C or 41°C. A total of 1000 immature oocytes were collected at slaughter from indigenous South African cow ovaries. The COC were randomly allocated (100/treatment) into 2 maturation times (18 or 24 h) and cultured in M199 + FSH-LH-estradiol medium under oil at 100% humidity and 5% CO2 at 39°C or 41°C. Post maturation, oocytes were subjected to normal subsequent embryo conditions. The Bracket and Oliphant medium was used for IVF. All matured oocytes were fertilised for 6 h with frozen-thawed Nguni bull semen at a concentration of 265 × 106. The presumptive zygotes from each treatment were cultured into SOF-BSA medium under oil and incubated at 39°C for assessment of cleavage rate 48 h post IVF. After Day 7 of culture, blastocyst were stained (Hoechst 33323) for nuclei cell count. Statistical analyses was performed using Genstat® software of SAS (SAS Institute, Cary, NC, USA; P < 0.05). Oocytes that were matured for 18 h in 41°C resulted in more 8-cell embryos (41%) compared with those incubated at 39°C (21.6%). However, no difference was observed for cleavage rate at both maturation times and incubation temperatures (41 or 39°C). There was more morula formation from oocytes matured for 18 h (19.6%) and 24 h (19.0%) at 41°C compared to 39°C (8.4%) group. The results further showed more blastocyst formation during 18 h at 41°C (15.2%) than at 39°C (7.4%) and during 24 h at 41°C (11.2%), 39°C (11.4%). However there was no difference in the nuclei cell number during 18 h at 41°C (45.2), 24 h (45.8), and 18 h at 39°C (43.4) of maturation. Thus, there was a significant difference in the nuclei cell numbers at 24 h on 39°C (n = 133.2) and 41°C (n = 45.8). In conclusion, oocytes that were matured for 18 and 24 h at 41°C or for 18 h at 39°C developed further to blastocyst stage on in vitro embryo production, however, with low nuclei cell numbers due to accelerated maturation temperature or shortened maturation period.

256 HOXB9 KNOCK-DOWN DURING OOCYTE MATURATION IN CATTLE

2013 ◽  
Vol 25 (1) ◽  
pp. 275
Author(s):  
D. Paul ◽  
L. Bridoux ◽  
R. Rezsöhazy ◽  
I. Donnay
Keyword(s):  
Oocyte Maturation ◽  
Blastocyst Stage ◽  
Differential Staining ◽  
Main Body ◽  
Post Injection ◽  
Immature Oocytes ◽  
Knock Down ◽  
Nuclear Maturation ◽  
The Mean ◽  
Number Of Cells

HOXB9 is a transcription factor playing a major role in patterning the main body-axis of vertebrate embryos from the gastrulation stage. HOXB9 transcripts have been detected in cattle oocytes and early embryos (Paul et al. 2011 Mol. Reprod. Dev. 78, 436–449), but their role at those stages has remained unknown. The aim of the study was to inject small interfering RNA (siRNA) in immature oocytes to investigate HOXB9 functions during oocyte maturation and early development. Immature cow oocytes were injected with 7 pL of a HOXB9 siRNA mixture (Integrated DNA Technologies, Coralville, IA, USA) or with a negative control siRNA (SCR) or were not injected (control). To allow microinjection, the size of the cumulus was reduced by pipetting; however, the corona radiata was left intact. The efficiency of the knock-down was measured 24 h post-injection using RT-qPCR, and the results were normalized with an internal normalization factor corresponding to the mean expression of three housekeeping genes (Goossens et al. 2005 BMC Dev. Biol. 5, 27). The injection of HOXB9 siRNA induced a 60.7% reduction of HOXB9 relative expression in mature oocytes by comparison with the injection of SCR siRNA (Kruskall-Wallis; P < 0.05). The nuclear maturation rates, assessed 24 h post-injection after Hoechst staining, were not statistically different between the HOXB9 group (48.8%) and the SCR group (53.6%; chi-square; P > 0.05). After in vitro fertilization and culture (Paul et al. 2011 Mol. Reprod. Dev. 78, 436–449), the developmental rates of the HOXB9 and the SCR groups were not statistically different in terms of achieving the first cleavage (64.4% v. 57.0%, respectively) and 5- to 8-cell stages (25.1% v. 25.3%, respectively) at 48 h post-insemination and the blastocyst stage on Day 7 (IVF = Day 0; 22.0% v. 20.6%, respectively) and Day 8 (26.9% v. 23.1%, respectively; two-way ANOVA; P > 0.05). Differential staining of Day 8 blastocysts showed no difference between the HOXB9 and the SCR groups regarding the mean number of cells in the inner cell mass (53.9 v. 49.4, respectively), the trophectoderm (125.5 v. 104.3, respectively), or the total number of cells (two-way ANOVA; P > 0.05). However, the microinjection procedure impaired the developmental competence after fertilization of the injected oocytes (HOXB9 + SCR) compared with the non-injected ones, as shown by their lower ability to cleave (60.7% v. 72.1%, respectively), to reach the 5- to 8-cell stage (25.2% v. 35.1%, respectively) or the blastocyst stage on Day 7 (21.3% v. 29.8%, respectively) and Day 8 (25.0% v. 35.8%, respectively; two-way ANOVA; P < 0.05). The injection did not affect blastocyst cell numbers on Day 8 (two-way ANOVA; P > 0.05). In conclusion, microinjection of siRNA in immature oocytes is a valuable method to efficiently knock down the maternal messenger RNA during oocyte maturation. Indeed, HOXB9 knock-down in immature oocytes led to a significant and important reduction in the amount of transcript in mature oocytes. The absence of effect of the knock-down on nuclear maturation and embryo development could be explained by the persistence of the maternal proteins. This work was funded by the Fonds National de la Recherche Scientifique (Belgium).

19 Invitro embryonic development from oocytes collected by ovum pickup of superstimulated females and nonstimulated slaughterhouse ovaries of alpaca (Vicugna pacos)

2021 ◽  
Vol 33 (2) ◽  
pp. 117
Author(s):  
L. Landeo ◽  
M. Zuñiga ◽  
T. R. Gastelu ◽  
J. A. Ruiz
Keyword(s):  
Embryonic Development ◽  
Statistical Significance ◽  
Calf Serum ◽  
Oocyte Quality ◽  
Blastocyst Stage ◽  
Cleavage Rate ◽  
Hormonal Stimulation ◽  
Array Transducer ◽  
The Mean

The objective of this study was to evaluate the embryonic development of alpaca oocytes collected by ovum pickup from superstimulated females (OPU, Group 1) and from slaughterhouse ovaries of 8 non-superstimulated females (SHO, Group 2) using a conventional aspiration technique (20G needle and a 3-mL syringe). A total of 8 nonpregnant alpacas, 3 to 4 years old, were superstimulated with a single dose of 200IU of equine chorionic gonadotrophin (eCG, Day=0). Three days later, alpacas were examined by transrectal ultrasonography with a 7.5-MHz linear-array transducer to determine the number and diameter of follicles available for aspiration. A total of 101 follicles were aspirated, recovering 67 oocytes (66.3%) by OPU using an endocavity transducer attached to a 21G needle adapted for alpacas. The follicular fluid was aspirated using a regulated vacuum pump (40 mmHg) into a tube containing 5mL of phosphate-buffered saline (PBS), 0.2% bovine serum albumin (BSA), and 10IUmL−1 heparin, at 37°C. In the SHO group we used 16 ovaries maintained at 28°C. The recovery of oocytes was carried out within 3h of ovary collection. We aspirated 155 follicles from SHO and recovered 117 oocytes (75.5%). After collection, all oocytes recovered were morphologically classified into categories (I and II) and cultured for 26h in an incubator (5% CO2 in air at 38.5°C), in TCM-199 supplemented with 0.2mmol sodium pyruvate, 50µgmL−1 gentamicin sulphate, 0.02IUmL−1 FSH, 1µgmL−1 oestradiol 17β, and 10% fetal calf serum (FCS). After maturation, oocytes were invitro fertilized with epididymal spermatozoa recovered from postmortem males and co-cultured for 18 to 20h. After this period, all cleaved oocytes were incubated (5% CO2 in air, 38.5°C) for 6 days in synthetic oviductal fluid-serum medium. Number and morphological quality of oocytes collected, invitro cleaved, and embryos ratea were registered and compared between groups. Statistical significance was determined using Kruskal–Wallis test. The mean and standard error were calculated from average of the percentages obtained in each repetition. Results indicated that the mean number of oocytes collected per ovary was higher (P&lt;0.05) using SHO (7.8±2.4) than OPU (4.5±3.0). Also, the number of oocytes classified as category I, was higher in the SHO compared with OPU group (56% vs. 30% respectively; P&lt;0.05); however, category II oocytes were the same (16% vs. 15%, respectively). There was no difference in early development (cleavage) rate between OPU (57±2.0) and SHO (49±1.5) groups. However, there was difference in the rate of development (P&lt;0.05) between OPU and SHO groups to reach the morula stage (56±2.0 vs. 42±1.7, respectively) and early blastocyst stage (55±2.0 vs. 34±1.4, respectively). In conclusion, oocyte quality could be affected by hormonal stimulation or by the quality of follicles aspirated by OPU. In contrast, oocytes recovered from live animals by OPU have greater capability of embryonic development invitro than oocytes recovered from slaughterhouse ovaries.

A simplified biopsy method for precompacted mouse embryos: A technical report

2002 ◽  
Vol 50 (4) ◽  
pp. 469-479 ◽  
Author(s):  
S. Bodó ◽  
L. Laczkó ◽  
Gabriella Horváth ◽  
Keyword(s):  
Genetic Diagnosis ◽  
In Utero ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Embryo Biopsy ◽  
Control Groups ◽  
Technical Report ◽  
Significant Difference ◽  
Biopsy Method

This article presents a new, simple and rapid embryo biopsy method. The blastomere for genetic analysis can be separated from a precompacted mouse embryo after a partial zona digestion with the use of a holding pipette. For the micromanipulation only two microcapillaries and micromanipulators are needed. The development of the biopsied embryos was studied during in vitro culture and in utero following embryo transfer. There was no significant difference between the treated and the control groups in the ratio of embryos that developed to the blastocyst stage, although the biopsied embryos were delayed in their development because they contained significantly fewer cells compared to the control ones at the same stage. Although there was no difference in the ratio of implantation, the development of the biopsied embryos in utero was also delayed 12-24 hours on the 9th day of pregnancy. No difference in development was visible from the 13th day of pregnancy. Statistically, no differences were found in the developmental ratio (number of developed fetuses/transferred embryos) of the control and treated embryos during gastrulation (9th day of pregnancy), at the beginning of organogenesis (13th day of pregnancy) and before birth (19th day of pregnancy). The embryo biopsy method presented here can be a new and useful tool for preimplantation genetic diagnosis.

scholarly journals Morphologic changes in boar sperm nuclei with reduced disulfide bonds in electrostimulated porcine oocytes

Reproduction ◽  
2006 ◽  
Vol 131 (3) ◽  
pp. 603-611 ◽  
Author(s):  
Michiko Nakai ◽  
Naomi Kashiwazaki ◽  
Akiko Takizawa ◽  
Naoki Maedomari ◽  
Manabu Ozawa ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Blastocyst Stage ◽  
Blastocyst Formation ◽  
The Mean ◽  
Sperm Nuclei ◽  
Morphologic Changes ◽  
Number Of Cells ◽  
Stimulation Of ◽  
Nuclear Decondensation

In pigs, failure of sperm nuclear decondensation has been reported after injection into oocytes. We examined the effects of pretreating sperm heads with Triton X-100 (TX-100) and dithiothreitol (DTT) and of electrical stimulation of oocytes after sperm head injection on time-dependent morphologic changes in sperm nuclei andin vitrodevelopment to the blastocyst stage. In experiment 1, spermatozoa were pretreated with 1% TX-100 and 5 mM DTT (T + D) or not treated, and then injected intoin vitromatured oocytes. Electrical stimulation (1.5 kV/cm, 20 μs DC pulse) was applied to the oocytes 1 h after injection (stimulated group) or was not applied (unstimulated group). Some of the oocytes in each group were evaluated at hourly intervals until 10 h after injection for morphologic changes in the sperm nuclei. Unstimulated oocytes injected with untreated spermatozoa showed a delayed peak in the rate of nuclear decondensation (39.4–44.1%, 3–6 h after injection) compared with oocytes injected with T + D-treated spermatozoa (57.0% and 52.6%, 1 and 2 h, respectively). The rate of male pronucleus formation peaked 6 h after stimulation (by 40–60%) after injected oocytes had been stimulated with an electrical pulse, irrespective of whether or not the spermatozoa had been pretreated. In unstimulated oocytes, the rate of male pronucleus formation did not increase and stayed at the basal level (less than 20%) throughout the culture period, regardless of the sperm treatment. Thus, T + D treatment of spermatozoa did not affect completion of fertilization. In experiment 2, we evaluated the effects of electrical stimulation and sperm treatment with T + D on the rate of blastocyst formation and the mean number of cells per blastocyst. Oocytes stimulated after injection with either T + D-treated or untreated spermatozoa showed significantly higher percentages of blastocyst formation (24.8% and 27.1% respectively) than did unstimulated oocytes (1.1% and 4.1% for T + D-treated and untreated respectively;P< 0.01 by Duncan’s multiple-range test). The rate of blastocyst formation did not differ between the T + D-treated and untreated groups. The mean number of cells per blastocyst did not differ among any of the groups (14.0–29.4 cells). These results suggest that pretreatment of sperm with TX-100 and DTT shifted the timing of sperm nuclear decondensation forward. However, pronucleus formation and development to the blastocyst stagein vitrowere not improved by sperm treatment. Thus, electrical stimulation of injected oocytes enhancesin vitrodevelopment to the blastocyst stage in pigs.

Early embryology af the marsupials Isoodon macrourus and Perameles nasuta

1976 ◽  
Vol 24 (3) ◽  
pp. 361 ◽  
Author(s):  
AG Lyne ◽  
DE Hollis
Keyword(s):  
Electron Microscopy ◽  
Outer Surface ◽  
Zona Pellucida ◽  
Light And Electron Microscopy ◽  
Blastocyst Stage ◽  
Embryonic Cells ◽  
Crystalloid Inclusions ◽  
Early Mouse ◽  
Number Of Cells ◽  
Rabbit Embryos

Twelve embryos, ranging from a four-celled stage to late unilaminar blastocysts, were obtained from the bandicoots I. macrourus and P. nasuta and examined by light and electron microscopy. These early stages covered at least one-quarter of the 12.5-day gestation period. The three non-cellular egg membranes characteristic of marsupials (zona pellucida, mucoid coat and shell membrane) were present, although the zona was sometimes absent or discontinuous in the intermediate and late unilaminar blastocysts examined. At the four-celled stage the blastomeres were close to the zona, but they had lost contact with each other, probably due to the extrusion of yolk, a phenomenon which has been described in other marsupials. The embryo did not increase in diameter until it was composed of at least 75 contiguous cells, which were in contact with the zona. In several of the larger blastocysts the protoderm cells had lost contact with the zona. Subsequently, the number of cells increased considerably and they were flattened against the egg membranes to form the late unilaminar blastocyst stage. Electron microscopy of the protoderm cells revealed the presence of numerous microvilli, particularly on the outer surface, and a range of other structures as great as those found in eutherian mammals. Remnants of spindle bridges were common in one 75-celled embryo. The yolk material in the blastocoele was also composed of a variety of structures, including small crystalloid inclusions composed of hexagonal units about 8-10 nm in diameter. Similar crystalloids have been described in the cells of early mouse and rabbit embryos and in egg and embryonic cells of various amphibians.

scholarly journals Development to Blastocyst Stage of Pig Oocytes Matured, Fertilized and Electroactivated In Vitro

2002 ◽  
Vol 45 (6) ◽  
pp. 547-556
Author(s):  
N. R. Mtango ◽  
M. D. Varisanga ◽  
D. Y. Juan ◽  
P. Wongrisekeao ◽  
T. Suzuki
Keyword(s):  
In Vitro Maturation ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Cleavage Rate ◽  
Vitro Fertilization ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
Activation Rates ◽  
Oviduct Fluid

Abstract. This study was designed 1) to determine the effectiveness of two in vitro maturation (IVM) media (tissue culture medium [TCM] and modified synthetic oviduct fluid supplemented with amino acids [mSOFaa]), 2) to compare the effects of two in vitro fertilization (IVF) media (modified Tris-buffered medium [mTBM] and mSOFaa) on the developmental competence of pig oocytes, and 3) to test the activation ability of IVM pig oocytes matured in TCM or mSOFaa, electroactivated and cultured in mSOFaa. The nuclear maturation rates were similar between IVM media (91.0 % vs. 89.0 %). A similar result was obtained when the activation rates were 54.2 % in TCM and 56.0 % in mSOFaa, and the blastocyst rates were 7.9 % and 6.1 %, respectively. There was no significant difference between mSOFaa and mTBM in the percentage of embryos with two pronuclei 33.2 % vs. 13.8 % or polypronuclei 5.3 % vs. 13.4 %. The cleavage rate was the same in both media. The medium mSOFaa gave a significantly higher (P< 0.05) blastocyst rate than mTBM (12.7 % vs. 3.9 %). We concluded that mSOFaa can enhance in vitro maturation, fertilization and culture of pig oocytes.

Sign in / Sign up

Export Citation Format

Share Document