Quantitative measurement of mammalian chromosome mitotic loss rates using the green fluorescent protein
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We have measured the mitotic loss rates of mammalian chromosomes in cultured cells. The green fluorescent protein (GFP) gene was incorporated into a non-essential chromosome so that cells containing the chromosome fluoresced green, while those lacking it did not. The proportions of fluorescent and non-fluorescent cells were measured by fluorescence activated cell sorter (FACS) analysis. Loss rates ranged from 0.005% to 0.20% per cell division in mouse LA-9 cells, and from 0.02% to 0.40% in human HeLa cells. The rate of loss was elevated by treatment with aneugens, demonstrating that the system rapidly identifies agents which induce chromosome loss in mammalian cells.
2011 ◽
Vol 48
(1)
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pp. 13-18
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1998 ◽
Vol 140
(4)
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pp. 779-793
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1999 ◽
Vol 10
(6)
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pp. 2033-2050
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