Fine Structure of the Cytoplasm in Salivary Glands of Simulium

1967 ◽  
Vol 2 (1) ◽  
pp. 137-144
Author(s):  
H. C. MACGREGOR ◽  
J. B. MACKIE
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Nuclear Envelope ◽  
Salivary Glands ◽  
Rough Endoplasmic Reticulum ◽  
Salivary Secretion ◽  
Septate Junction ◽  
Cell Junctions ◽  
Salivary Gland Cell ◽  
Apical Cytoplasm

The salivary glands of 3rd or 4th instar larvae of Simulium niditifrons are about 5 mm long and up to 400 µ wide. They have a capacious lumen which is normally filled with secretion. The apical (luminal) plasmalemma of the gland cells is thrown into numerous microvilli. The basal plasmalemma is usually straight but is infolded in places. The infoldings may be complex near to cell junctions. There is a thick, uniform basement membrane. Contact surfaces of adjacent cells often interdigitate. A septate junction extends inwards from the lumen for one-quarter the depth of the cells. Rough endoplasmic reticulum is distributed evenly throughout the cytoplasm. Many Golgi complexes with dark membrane-bounded granules are scattered throughout the cytoplasm. Solitary granules, often more than I µ in diameter, lie in the apical cytoplasm, especially near the apical border of the cell. These granules resemble the larger Golgi granules and the contents of the lumen. Solitary granules consisting of 2 components have been seen in various stages of passage through the cell membrane. The 2 components are present in roughly constant proportions and can be identified in the larger Golgi granules and in the secretion in the lumen. The nucleus is spherical. The nuclear envelope is smooth in the larger cells of a gland but may be folded in the smaller cells. There are 80-100 pores/µ2 of nuclear envelope. Each pore appears to have a small granule at its centre. Microtubules, about 180 Å thick, are numerous in the apical cytoplasm, particularly near the luminal border. Tubules which lie deep in the cytoplasm are flanked by a clear area 100-200 Å wide. The fine structure of a salivary gland cell of Simulium appears to indicate that the major components of the salivary secretion are synthesized in association with the ribosomes on the rough endoplasmic reticulum, concentrated in the Golgi regions, formed into secretion granules, and passed out of the cell into the lumen of the gland by reverse phagocytosis.

scholarly journals DEVELOPMENT OF ENDOGENOUS PEROXIDASE IN FETAL RAT SUBMANDIBULAR GLAND

1973 ◽  
Vol 21 (1) ◽  
pp. 42-50 ◽  
Author(s):  
SHOHEI YAMASHINA ◽  
TIBOR BARKA
Keyword(s):  
Endoplasmic Reticulum ◽  
Nuclear Envelope ◽  
Submandibular Gland ◽  
Rough Endoplasmic Reticulum ◽  
Peroxidase Activity ◽  
Secretory Granules ◽  
Prenatal Development ◽  
Reaction Products ◽  
Cell Type ◽  
Endogenous Peroxidase

The prenatal development of endogenous peroxidase activity in the submandibular gland of rat was investigated by means of the diaminobenzidine-H2O2 histochemical method. The submandibular gland of a 16-day-old fetus was composed of cords of uniform, undifferentiated cells which contained no secretory granules and revealed no peroxidase activity. Peroxidase activity first appeared at the 17th day of gestation in the cisternae of the rough endoplasmic reticulum and nuclear envelope in a few cells. At the 18th day of gestation cells which exhibited reaction products in the rough endoplasmic reticulum and nuclear envelope also contained secretory granules with a strong peroxidase activity. During the last days of gestation the number of peroxidase positive cells, which contained numerous secretory granules, increased. The peroxidase-containing cells are the immediate precursors of the proacinar cells of early postnatal stages. During the same time period, when the peroxidase-containing cells differentiated, a second cell type also differentiated in the cellular cords. The development of this cell type was marked by the appearance of secretory granules stainable with toluidine blue. Through the prenatal development, this cell type revealed no peroxidase activity and was identified with the terminal tubule cell of the newborn. The morphologic and cytochemical findings indicate that terminal tubule cells and proacinar cells are committed cells; the former differentiate toward 2nd order intercalated duct cells and the latter transform to mature acinar cells.

Anatomy, Histology, and Ultrastructure of Salivary Glands of the Burrower Bug, Scaptocoris castanea (Hemiptera: Cydnidae)

2019 ◽  
Vol 25 (6) ◽  
pp. 1482-1490 ◽  
Author(s):  
Jamile Fernanda Silva Cossolin ◽  
Luis Carlos Martínez ◽  
Monica Josene Barbosa Pereira ◽  
Lucia Madalena Vivan ◽  
Hakan Bozdoğan ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Rough Endoplasmic Reticulum ◽  
Accessory Gland ◽  
Excretory Duct ◽  
Secretory Cells ◽  
Morphological Description ◽  
Basal Cells ◽  
Narrow Lumen

AbstractThe burrower bug Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae) is an agricultural pest feeding on roots of several crops. The histology and ultrastructure of the salivary glands of S. castanea were described. The salivary system has a pair of principal salivary glands and a pair of accessory salivary glands. The principal salivary gland is bilobed with anterior and posterior lobes joined by a hilus where an excretory duct occurs. The accessory salivary gland is tubular with a narrow lumen that opens into the hilus near the excretory duct, suggesting that its secretion is stored in the lumen of the principal gland. The cytoplasm of the secretory cells is rich in the rough endoplasmic reticulum, secretory vesicles with different electron densities and mitochondria. At the base of the accessory gland epithelium, there were scattered cells that do not reach the gland lumen, with the cytoplasm rich in the rough endoplasmic reticulum, indicating a role in protein production. Data show that principal and accessory salivary glands of S. castanea produce proteinaceous saliva. This is the first morphological description of the S. castanea salivary system that is similar to other Hemiptera Pentatomomorpha, but with occurrence of basal cells in the accessory salivary gland.

scholarly journals OBSERVATIONS ON THE DEVELOPMENT OF ERYTHROCYTES IN MAMMALIAN FETAL LIVER

1962 ◽  
Vol 14 (2) ◽  
pp. 235-254 ◽  
Author(s):  
Joseph A. Grasso ◽  
Hewson Swift ◽  
G. Adolph Ackerman
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Nuclear Envelope ◽  
Cell Size ◽  
Marked Decrease ◽  
Fetal Liver ◽  
Hepatic Parenchyma ◽  
Hemoglobin Synthesis ◽  
Hepatic Cells ◽  
Eventual Loss

The fine structure of the erythrocyte during development in rabbit and human fetal liver has been studied. A morphologic description of representative erythropoietic cells and their relationship to the hepatic parenchyma is presented. Erythrocyte development was accompanied by a decrease in nuclear and cell size, fragmentation and eventual loss of nucleoli, and progressive clumping of chromatin at the nuclear margin. Mitochondria, endoplasmic reticulum, and Golgi elements decreased in size or abundance and eventually disappeared. Ribosome concentration initially increased, but subsequently diminished as the cytoplasm increased in electron opacity, probably through the accumulation of hemoglobin. Similar dense material, interpreted to be hemoglobin, infiltrated the nuclear annuli and, in some cases, appeared to extend into the interchromatin regions. There was a marked decrease in the number of annuli of the nuclear envelope. Possible relationships between nucleus and cytoplasm and of RNA to hemoglobin synthesis are discussed. In rabbits, erythroid and hepatic cells were separated by a 200 to 400 A space limited by the undulatory membranes of the respective cells. Membranes of adjacent erythropoietic cells were parallel and more closely apposed (100 to 200 A). In humans, relationship between various cells exhibited wide variation. Ferritin particles were observed within forming and formed "rhopheocytotic" vesicles.

The fine structure of the leucocytes of the holothurian, Cucumaria miniata

1977 ◽  
Vol 55 (9) ◽  
pp. 1530-1544 ◽  
Author(s):  
A. R. Fontaine ◽  
Philip Lambert
Keyword(s):  
Stem Cells ◽  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Rough Endoplasmic Reticulum ◽  
Acid Mucopolysaccharide ◽  
Rough Er

The fine structure of amoebocytes, lymphocytes, and morula cells is described and related to their functions. Three morphological phases (bladder, transitional, and filiform) of the amoebocyte are distinguished. Their surface protrusions and activities are based on microtubule and microfilament systems and the transitional-filiform phases are functionally involved in coelomocyte aggregation. The bladder phase is phagocytic; bladder formation and activities are also microfilament based. Morula cells contain spherules composed of acid mucopolysaccharide and protein. Dilated rough endoplasmic reticulum (ER) cisternae apparently synthesize spherule material which is added by accretion. Lymphocytes have little cytoplasm and relatively few organelles, except for abundant rough ER and free ribosomes. Lymphocytes are probably stem cells for amoebocytes and morulas. These cells are compared with the leucocytes of other echinoderms.

scholarly journals FINE STRUCTURE AND ORGANELLE ASSOCIATIONS IN BROWN ALGAE

1965 ◽  
Vol 26 (2) ◽  
pp. 523-537 ◽  
Author(s):  
G. Benjamin Bouck
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Golgi Apparatus ◽  
Nuclear Envelope ◽  
Brown Algae ◽  
Algal Cell ◽  
Plant Cells ◽  
Membrane Systems ◽  
Two Envelopes

The structural interrelationships among several membrane systems in the cells of brown algae have been examined by electron microscopy. In the brown algae the chloroplasts are surrounded by two envelopes, the outer of which in some cases is continuous with the nuclear envelope. The pyrenoid, when present, protrudes from the chloroplast, is also surrounded by the two chloroplast envelopes, and, in addition, is capped by a third dilated envelope or "pyrenoid sac." The regular apposition of the membranes around the pyrenoid contrasts with their looser appearance over the remainder of the chloroplast. The Golgi apparatus is closely associated with the nuclear envelope in all brown algae examined, but in the Fucales this association may extend to portions of the cytoplasmic endoplasmic reticulum as well. Evidence is presented for the derivation of vesicles, characteristic of those found in the formative region of the Golgi apparatus, from portions of the underlying nuclear envelope. The possibility that a structural channeling system for carbohydrate reserves and secretory precursors may be present in brown algae is considered. Other features of the brown algal cell, such as crystal-containing bodies, the variety of darkly staining vacuoles, centrioles, and mitochondria, are examined briefly, and compared with similar structures in other plant cells.

The Fine Structure of Primordial Germ Cells of the Rat

1973 ◽  
Vol 31 ◽  
pp. 634-635
Author(s):  
E. M. Eddy
Keyword(s):  
Endoplasmic Reticulum ◽  
Life History ◽  
Fine Structure ◽  
Rough Endoplasmic Reticulum ◽  
Germ Cells ◽  
Fibrous Material ◽  
Primordial Germ Cells ◽  
The Other ◽  
Large Size ◽  
History Of

Primordial germ cells are readily recognizable in embryos of the rat due to their large size, generally rounded shape and prominent nuclei with uniformly dispersed heterochromatin. They often have blunted pseudopodal processes at one end and small ruffles or trailing processes at the other, characteristics expected from their known ameboid activity- and migratory abilities. Also, the cytoplasm is rich in polyribosomes and contains a modest amount of rough endoplasmic reticulum and the mitochondria are frequently larger and less dense than those of adjacent somatic cells.In addition to these general characteristics, there are features unique to germ cells which allow them to be identified with certainty. These are: 1) small vesicles containing an irregular, dense core and 2) discrete accumulations of fibrous material known as nuage. Both of these features are present in other species and at other times in the life history of germ cells. The dense-cored vesicles have been noted in fetal and early postnatal mouse oogonia and oocytes, and in hamster and rabbit oocytes.

The Role of Endoplasmic Reticulum in the Repair of Amoeba Nuclear Envelopes Damaged Microsurgically

1974 ◽  
Vol 14 (2) ◽  
pp. 421-437
Author(s):  
C. J. FLICKINGER
Keyword(s):  
Endoplasmic Reticulum ◽  
Nuclear Envelope ◽  
Rough Endoplasmic Reticulum ◽  
Repair Process ◽  
Nuclear Membranes ◽  
Normal Configuration ◽  
Layer Characteristic ◽  
The Relationship ◽  
Coated Slide

The nuclear envelopes of amoebae were damaged microsurgically, and the fate of the lesions was studied with the electron microscope. Amoebae were placed on the surface of an agar-coated slide. Using a glass probe, the nucleus was pushed from an amoeba, damaged with a chopping motion of the probe, and reinserted into the amoeba. Cells were prepared for electron microscopy at intervals of between 10 min and 4 days after the manipulation. Nuclear envelopes studied between 10 min and 1 h after the injury displayed extensive damage, including numerous holes in the nuclear membranes. Beginning 15 min after the manipulation, pieces of rough endoplasmic reticulum intruded into the holes in the nuclear membranes. These pieces of rough endoplasmic reticulum subsequently appeared to become connected to the nuclear membranes at the margins of the holes. By 1 day following the injury, many cells had died, but the nuclear membranes were intact in those cells that survived. The elaborate fibrous lamina or honeycomb layer characteristic of the amoeba nuclear envelope was resistant to early changes after the manipulation. Patches of disorganization of the fibrous lamina were present 5 h to 1 day after injury, but the altered parts showed evidence of progress toward a return to normal configuration by 4 days after the injury. It is proposed that the rough endoplasmic reticulum participates in the repair of injury to the nuclear membranes. The similarity of this repair process to reconstitution of the nuclear envelope in telophase of mitosis is noted, and the relationship between the nuclear envelope and the rough endoplasmic reticulum is discussed.

scholarly journals Ultrastructure of mononuclear phagocytes developing in liquid bone marrow cultures. A study on peroxidatic activity

1979 ◽  
Vol 149 (1) ◽  
pp. 17-26 ◽  
Author(s):  
JWM Van Der Meer ◽  
RHJ Beelen ◽  
DM Fluitsma ◽  
R Van Furth
Keyword(s):  
Bone Marrow ◽  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Nuclear Envelope ◽  
Rough Endoplasmic Reticulum ◽  
Precursor Cells ◽  
Mononuclear Phagocytes ◽  
Peroxidatic Activity

Monoblasts, promonocytes, and macrophages in in vitro cultures of murine bone marrow were studied ultrastructurally, with special attention to peroxidatic activity. Monoblasts show peroxidatic activity in the rough endoplasmic reticulum and nuclear envelope as well as in the granules. The presence of peroxidatic activity in the Golgi apparatus could not be determined. Promonocytes have peroxidase-positive rough endoplasmic reticulum, Golgi apparatus, nuclear envelope, and granules, as previously reported. During culture, cells are formed with peroxidatic activity similar to that of monocytes or exudate macrophages (positive granules; negative Golgi apparatus, RER, and nuclear envelope); we call these cells early macrophages. In addition, transitional macrophages with both positive granules and positive RER, nuclear envelope, negative Golgi apparatus (as in exudate- resident macrophages in vivo), and mature macrophages with peroxidatic activity only in the RER and nuclear envelope (as in resident macrophages in vivo) were found. A considerable number of cells without detectable peroxidatic activity were also encountered. Our finding that macrophages with the peroxidatic pattern of monocytes (early macrophages), exudate-resident macrophages (transitional macrophages), and resident macrophages (mature macrophages), develop in vitro from proliferating precursor cells deriving from the bone marrow, demonstrates once again that resident macrophages in tissues originate from precursor cells in the bone marrow. Therefore, this conclusion can no longer be challenged on the basis of a cytochemical difference between monocytes and exudate macrophages on the one hand and resident macrophages on the other.

On the morphology and fine structure of the alimentary canal of Corophium volutator (Pallas) (Crustacea: Amphipoda)

1984 ◽  
Vol 306 (1126) ◽  
pp. 49-78 ◽  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
B Cells ◽  
Rough Endoplasmic Reticulum ◽  
Digestive Enzymes ◽  
Dense Matrix ◽  
Corophium Volutator ◽  
Pyloric Region ◽  
Anterior Dorsal ◽  
Apical Complex

In the mud-dwelling amphipod, Corophium volutator the foregut is lined with cuticle and consists of an oesophagus and a stomach, with the latter divided into cardiac, pyloric and funnel regions. The midgut comprises an intestine that is enlarged considerably by three pairs of diverticula: the small anterior dorsal and posterior caeca and the massive ventral caeca. Anteriorly, the intestine encompasses the funnel region and the ventral caeca open into the floor of the stomach at the posterior end of the pyloric region. The hindgut is essentially a simple tube connnecting the intestine with the anus. Particles of food pass along the oesophagus and enter the stomach through a valve. Rows of setae, or folds of cuticle, divide the stomach longitudinally into food, circulation and filtration channels. Ingested particles with a diameter greater than 2 pm are confined to the food channel and supplied with fluids and enzymes from the circulation channels. The digestive enzymes are produced primarily by the ventral caeca and are supplied to the circulation channels through a valve at the entrance of each ventral caecum. Any fine particles and soluble materials extracted from the food channel in the cardiac region are transported into the filtration channels through the first filter of a two part system. Digestible material continues to be extracted in the pyloric region where the volume of the lumen of the food channel is reduced by the intrusion of the vertex of the ventral pyloric ridge. The basis of this ridge supports the second filter which produces a filtrate with particles less than 0.06 pm in diameter. Material retained on the filter membrane is returned to the food channel by brush-like setae facing the membrane. The final filtrate is transported to the ventral caeca. A valve at the entrance to each ventral caecum prevents contamination of the filtrate by material in the food channel. All indigestible food is passed sequentially along the funnel, intestine and, finally, the hindgut from which it is voided as a faecal pellet. Most digestion and absorption occur in the ventral caeca where the epithelium is differentiated into the R /F and B cells. The R /F cells have a much thicker and denser microvillous border than the B cells. Each R /F cell also has numerous mitochondria located mainly ventral to the nucleus in the mid-region. Rough and smooth endoplasmic reticula are sited primarily in the apical and basal regions of the cell, respectively. Furthermore, most of the rough endoplasmic reticulum is confined to cells in the distal region of the caecum which probably forms the main site for the production of digestive enzymes. The proximal region of the caecum contains numerous lipid droplets and is probably involved in the absorption, transport and storage of the products of digestion. Each B cell has a single large, fluid-filled vacuole, distal to which are mitochondria and numerous smaller vacuoles of varying size forming an ‘apical complex’. The nucleus is located proximal to the vacuole together with free ribosomes and rough endoplasmic reticulum. Material from the lumen of the caecum is taken by pinocy tosis into the ‘apical complex’. The large vacuole develops at the expense of the ‘apical complex’ and the microvillous border. The vacuole is eventually liberated into the lumen of the caecum and the cell disintegrates. These discharges may supply enzymes to other regions of the gut, or they could be waste products derived from intracellular digestion. The anterior dorsal caeca and most of the intestine contain cells with a normal complement of organelles. These cells probably make a minor contribution to the processes of digestion and absorption. However, the cells of the posterior caeca and those at the posterior end of the intestine have an extensive development of smooth endoplasmic reticulum. In some cells the mitochondria have a dense matrix and there are only a few free ribosomes and cisternae of rough endoplasmic reticulum. The fine structure of the epithelium in the posterior caeca is typical of tissue that transports fluids and ions. The hindgut has a microvillous border which abuts its cuticular lining. In addition, some cells have numerous mitochondria which are often associated with infolds of the basal cell membrane. The fine structure of this tissue is similar to the ‘ion pumps’ described in the gut of insects which serve to maintain the normal ionic concentration of the blood. The posterior region of the hindgut has no structural specializations.

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