Anatomy, Histology, and Ultrastructure of Salivary Glands of the Burrower Bug, Scaptocoris castanea (Hemiptera: Cydnidae)

2019 ◽  
Vol 25 (6) ◽  
pp. 1482-1490 ◽  
Author(s):  
Jamile Fernanda Silva Cossolin ◽  
Luis Carlos Martínez ◽  
Monica Josene Barbosa Pereira ◽  
Lucia Madalena Vivan ◽  
Hakan Bozdoğan ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Rough Endoplasmic Reticulum ◽  
Accessory Gland ◽  
Excretory Duct ◽  
Secretory Cells ◽  
Morphological Description ◽  
Basal Cells ◽  
Narrow Lumen

AbstractThe burrower bug Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae) is an agricultural pest feeding on roots of several crops. The histology and ultrastructure of the salivary glands of S. castanea were described. The salivary system has a pair of principal salivary glands and a pair of accessory salivary glands. The principal salivary gland is bilobed with anterior and posterior lobes joined by a hilus where an excretory duct occurs. The accessory salivary gland is tubular with a narrow lumen that opens into the hilus near the excretory duct, suggesting that its secretion is stored in the lumen of the principal gland. The cytoplasm of the secretory cells is rich in the rough endoplasmic reticulum, secretory vesicles with different electron densities and mitochondria. At the base of the accessory gland epithelium, there were scattered cells that do not reach the gland lumen, with the cytoplasm rich in the rough endoplasmic reticulum, indicating a role in protein production. Data show that principal and accessory salivary glands of S. castanea produce proteinaceous saliva. This is the first morphological description of the S. castanea salivary system that is similar to other Hemiptera Pentatomomorpha, but with occurrence of basal cells in the accessory salivary gland.

scholarly journals Ultrastructure of the Salivary Glands of the Stink Bug Predator Podisus distinctus

2015 ◽  
Vol 21 (6) ◽  
pp. 1514-1522 ◽  
Author(s):  
Luis C. Martínez ◽  
José C. Zanuncio ◽  
Wagner C.C. Morais ◽  
Angelica Plata-Rueda ◽  
Pedro E. Cedeño-Loja ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Rough Endoplasmic Reticulum ◽  
Apical Cell ◽  
Cell Region ◽  
Accessory Glands ◽  
Decondensed Chromatin ◽  
Membrane Infoldings

AbstractPodisus distinctus (Hemiptera: Pentatomidae) is a zoophytophagous insect with significant potential for use as a biological control agent in agriculture and forestry because their nymphs and adults actively prey on diverse insect species. The saliva of this insect possesses active substances that cause paralysis and death of the prey. As the first step in identifying compounds of P. distinctus saliva, this study describes the ultrastructure of the salivary glands of this predator. The salivary system of P. distinctus possesses a pair of main salivary glands with a short anterior lobe, a long posterior lobe, and a pair of tubular accessory glands. The main salivary gland of P. distinctus has no associated muscles, suggesting that the saliva-release mechanism occurs with the help of certain thorax muscles. The main salivary gland epithelium has a single layer of cells (varying from cubical to columnar) with cytoplasm rich in rough endoplasmic reticulum, spherical granules of different sizes, a nucleus with a predominance of decondensed chromatin, and nucleolus. The apical cell region has a few short microvilli and the basal region has plasma membrane infoldings. The epithelium of the accessory salivary glands possesses a single-layered epithelium of cubic cells delimiting a narrow lumen. The apical cell region has a high density of microvilli and pleomorphic mitochondria, whereas the central cell region is rich in rough endoplasmic reticulum with a well-developed nucleus and decondensed chromatin. The basal cell region is characterized by the presence of several basal plasma membrane infoldings associated with mitochondria and numerous openings to the hemocoel forming large channels. The ultrastructural characteristics suggest that the main salivary glands and accessory salivary glands play a vital role in protein synthesis for saliva production and that the accessory glands are involved in transport of materials of the hemolymph.

Simple mucin-type carbohydrate antigens in major salivary glands.

1994 ◽  
Vol 42 (9) ◽  
pp. 1251-1259 ◽  
Author(s):  
M H Therkildsen ◽  
U Mandel ◽  
J Thorn ◽  
M Christensen ◽  
E Dabelsteen
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Acinar Cells ◽  
Salivary Gland Tumors ◽  
Basal Cells ◽  
T Antigens ◽  
Glycosylation Pattern ◽  
Carbohydrate Antigens ◽  
Major Salivary Glands ◽  
Duct Cells

Simple mucin-type carbohydrate antigens Tn, sialosyl-Tn and T are often markers of neoplastic transformation and have very limited expression in normal tissues. We performed an immunohistological study of simple mucin-type carbohydrate antigens, including H and A variants, with well-defined monoclonal antibodies (MAb) on frozen and paraffin-embedded normal salivary gland tissue from 22 parotid, 14 submandibular, six sublingual, and 13 labial glands to elucidate the simple mucin-type glycosylation pattern in relation to cyto- and histodifferentiation. The investigated carbohydrate structures were predominantly observed in the cell cytoplasm, most often in the supranuclear area, suggesting localization to the Golgi region, whereas ductal contents were unstained. Mucous acinar cells expressed Tn, sialosyl-Tn, and H and A antigens, regardless of glandular location. Serous acinar cells, on the other hand, expressed A, H, and inconstantly sialosyl-T, Tn, and sialosyl-Tn antigens in major salivary glands, whereas serous cells of minor (labial) salivary glands expressed H exclusively, Tn and sialosyl-T antigens inconstantly, but never sialosyl-Tn and A antigens. The difference may be related to a more simple cytodifferentiation of serous cells of minor (labial) salivary glands as compared with major salivary glands. Duct cells in major salivary glands expressed A, H, and inconstantly T, sialosyl-T, and Tn antigens, whereas minor (labial) salivary glands ducts exclusively expressed H, T and sialosyl-T antigens, differences that may be related to dissimilarities in the duct system. Myoepithelial cells and basal cells exclusively expressed T and sialosyl-T antigens, which may prove useful in studies of salivary gland tumors, since these cells are known to play a key role in the histological characteristics of some salivary gland tumors. The results indicate a similar glycosylation pattern in the different major salivary glands, whereas minor (labial) salivary gland differ slightly in serous and duct cells. The limited and exclusive intracellular expression of the immature Tn, sialosyl-Tn, and T antigens indicates that these structures may be of value as markers of salivary gland tumors.

Fine Structure of the Cytoplasm in Salivary Glands of Simulium

1967 ◽  
Vol 2 (1) ◽  
pp. 137-144
Author(s):  
H. C. MACGREGOR ◽  
J. B. MACKIE
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Nuclear Envelope ◽  
Salivary Glands ◽  
Rough Endoplasmic Reticulum ◽  
Salivary Secretion ◽  
Septate Junction ◽  
Cell Junctions ◽  
Salivary Gland Cell ◽  
Apical Cytoplasm

The salivary glands of 3rd or 4th instar larvae of Simulium niditifrons are about 5 mm long and up to 400 µ wide. They have a capacious lumen which is normally filled with secretion. The apical (luminal) plasmalemma of the gland cells is thrown into numerous microvilli. The basal plasmalemma is usually straight but is infolded in places. The infoldings may be complex near to cell junctions. There is a thick, uniform basement membrane. Contact surfaces of adjacent cells often interdigitate. A septate junction extends inwards from the lumen for one-quarter the depth of the cells. Rough endoplasmic reticulum is distributed evenly throughout the cytoplasm. Many Golgi complexes with dark membrane-bounded granules are scattered throughout the cytoplasm. Solitary granules, often more than I µ in diameter, lie in the apical cytoplasm, especially near the apical border of the cell. These granules resemble the larger Golgi granules and the contents of the lumen. Solitary granules consisting of 2 components have been seen in various stages of passage through the cell membrane. The 2 components are present in roughly constant proportions and can be identified in the larger Golgi granules and in the secretion in the lumen. The nucleus is spherical. The nuclear envelope is smooth in the larger cells of a gland but may be folded in the smaller cells. There are 80-100 pores/µ2 of nuclear envelope. Each pore appears to have a small granule at its centre. Microtubules, about 180 Å thick, are numerous in the apical cytoplasm, particularly near the luminal border. Tubules which lie deep in the cytoplasm are flanked by a clear area 100-200 Å wide. The fine structure of a salivary gland cell of Simulium appears to indicate that the major components of the salivary secretion are synthesized in association with the ribosomes on the rough endoplasmic reticulum, concentrated in the Golgi regions, formed into secretion granules, and passed out of the cell into the lumen of the gland by reverse phagocytosis.

Ultrastructure and ontogeny of hair filaments in the subtidal saccate alga Hydroclathrus clathratus (Phaeophyta, Scytosiphonales)

1987 ◽  
Vol 65 (8) ◽  
pp. 1687-1693 ◽  
Author(s):  
Brian R. Oates ◽  
Kathleen M. Cole
Keyword(s):  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Brown Alga ◽  
Narrow Band ◽  
Chloroplast Envelope ◽  
Cell Periphery ◽  
Basal Cells ◽  
Hydroclathrus Clathratus

Hair filaments found in pits scattered over the surface of the saccate brown alga Hydroclathrus clathratus were examined ultrastructurally. Cells near the base of filaments are short and metabolically very active, containing many mitochondria, ribosomes, rough endoplasmic reticulum, and dictyosomes but no vacuoles. Numerous plasmodesmata connect cells of the filaments. The more distal cells elongate during development of the pits. As elongation begins, dictyosome activity dominates and vacuoles appear. Very elongate cells are filled almost exclusively by a vacuole with a narrow band of cytoplasm at the cell periphery. Chloroplasts are found only in basal cells of short filaments. Other cells near the filament base contain plastosomes, small spherical bodies derived from chloroplasts by a series of divisions. While morphologically distinct, plastosomes have retained many features of brown algal chloroplasts. They are bound by the chloroplast envelope and chloroplast endoplasmic reticulum and contain a ring genophore. The thylakoid system varies in that more than three thylakoids are usually loosely associated in the peripheral band and the interior bands are absent in more mature plastosomes.

Salivary Glands: A Paradigm for Diversity of Gland Development

1997 ◽  
Vol 8 (1) ◽  
pp. 51-75 ◽  
Author(s):  
P.C. Denny ◽  
W.D. Ball ◽  
R.S. Redman
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Expression Patterns ◽  
Cell Types ◽  
Secretory Cells ◽  
Control Mechanisms ◽  
Regulatory Cascade ◽  
Late Initiation ◽  
Cellular Phenotypes ◽  
Gland Development

The major salivary glands of mammals are represented by three pairs of organs that cooperate functionally to produce saliva for the oral cavity. While each type of gland produces a signature secretion that complements the secretions from the other glands, there is also redundancy as evidenced by secretion of functionally similar and, in some cases, identical products in the three glands. This, along with their common late initiation of development, in fetal terms, their similarities in developmental pattern, and their proximate sites of origin, suggests that a common regulatory cascade may have been shared until shortly before the onset of overt gland development. Furthermore, occasional ectopic differentiation of individual mature secretory cells in the "wrong" gland suggests that control mechanisms responsible for the distinctive cellular composition of each gland also share many common steps, with only minor differences providing the impetus for diversification. To begin to address this area, we examine here the origins of the salivary glands by reviewing the expression patterns of several genes with known morphogenetic potential that may be involved based on developmental timing and location. The possibility that factors leading to determination of the sites of mammalian salivary gland development might be homologous to the regulatory cascade leading to salivary gland formation in Drosophila is also evaluated. In a subsequent section, cellular phenotypes of neonatal and adult glands are compared and evaluated for insights into the mechanisms and lineages leading to cellular diversification. Finally, the phenomena of proliferation, repair, and regeneration in adult salivary glands are reviewed, with emphasis on the extent to which the cellular diversity is reversible and which cell type other than stem cells has the ability to redifferentiate into other cell types.

scholarly journals HUMAN WOUND REPAIR

1968 ◽  
Vol 39 (1) ◽  
pp. 152-168 ◽  
Author(s):  
Russell Ross ◽  
George Odland
Keyword(s):  
Endoplasmic Reticulum ◽  
Connective Tissue ◽  
Rough Endoplasmic Reticulum ◽  
Wound Repair ◽  
Mononuclear Cells ◽  
Basal Cells ◽  
Adult Males ◽  
Intimate Contact ◽  
Neutrophilic Leukocytes ◽  
Connective Tissue Repair

Connective tissue repair was studied in a series of skin wounds in young adult males. The tissues were examined at 3, 12, and 24 hr, and at 2, 3, 5, 7, 14, and 21 days after wounding. The neutrophilic leukocytes contain within membrane-bounded vacuoles some fibrin and serum protein from the wound; however, most of the granulocytes lyse and release their cytoplasmic contents into the extracellular space. The mononuclear cells undergo a series of morphologic alterations during which they develop a modest amount of relatively poorly developed rough endoplasmic reticulum and an extensive system of smooth-surfaced membranes prior to active phagocytosis. They could be clearly distinguished from immature fibroblasts by the differences in the development of their organelles, particularly the rough endoplasmic reticulum. The perivascular connective tissue adjacent to the wound contains cells which appear like poorly developed or immature fibroblasts. The development of these cells into mature fibroblasts can be followed during the different stages of wound repair. Intimate contact was observed between basal cells of the regenerated epidermis and monocytes in the wound below: cytoplasmic projections of the basal cells extended beneath the basement lamina to the surface of the monocytes. Such contacts were seen only on the 4th–7th day after wounding. Their possible significance is discussed.

scholarly journals Structural and Ultrastructural Morphological Evaluation of Giant Anteater (Myrmecophaga tridactyla) Prostate Gland

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 231
Author(s):  
Fernanda Moura ◽  
Letícia Sampaio ◽  
Priscila Kobayashi ◽  
Renee Laufer-Amorim ◽  
João Carlos Ferreira ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Prostate Gland ◽  
Specific Antigen ◽  
Species Conservation ◽  
Ultrastructural Feature ◽  
Secretory Cells ◽  
Morphological Description ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Young Subjects

The giant anteater (Myrmecophaga tridactyla) is a vulnerable species from Central and South America, and is considered possibly extinct in Belize, Guatemala, El Salvador, and Uruguay. Due to the species’ conservation and reproductive importance, this research aimed to characterize the morphology, histochemical, immunohistochemical, and ultrastructural feature of the giant anteater prostate gland. For this, we collected 11 giant anteater prostate glands and performed macroscopic, morphological, histochemical, immunohistochemical, and ultrastructural analysis. Nine prostate glands from an adult subject and two from young subjects were studied. Grossly, the adult giant anteater prostate gland is divided in two distinct zones; the central zones (composed mainly of ducts) and the peripheral zones (of acini formed by secretory cells). The secretory cells showed positive periodic acid–Schiff staining. Furthermore, the immunohistochemical characterization revealed a similar human prostate pattern, with p63 staining basal cells, uroplakin III (UPIII) superficial cells of prostatic urethra, androgen receptor (AR) expressing nucleus of secretory and stromal cells, and prostatic specific antigen (PSA) staining prostatic epithelial cells. Overall, our research provided an in-depth morphological description of the giant anteater’s prostate gland, providing valuable information for futures studies focused on giant anteater conservation.

Histology of the Parotid Gland

2021 ◽  
pp. 6-12
Keyword(s):  
Connective Tissue ◽  
Parotid Gland ◽  
Salivary Glands ◽  
Structural Changes ◽  
Myoepithelial Cells ◽  
Excretory Duct ◽  
Secretory Cells ◽  
Histological Features ◽  
Stensen’S Duct ◽  
Stensen's Duct

This chapter describes the characteristic histological features of the parotid gland. The microanatomy of the parotid gland includes the following: stroma (connective tissue) and parenchyma (secretory units or acini, myoepithelial cells, and ductal units). The secretory cells are organized into secretory units or acini, which are lined exclusively by serous cells. Myoepithelial cells are the contractile elements with secretory end pieces and intercalated ducts. The acini are drained by a series of ducts which eventually drain into the major excretory duct (Stensen's duct). Aging of salivary glands show some structural changes, but with no overall change in the amount of saliva secreted with advancing age.

scholarly journals THE LOCALIZATION OF ENDOGENOUS PEROXIDASE IN THE LACRIMAL GLAND OF THE RAT DURING POSTNATAL DEVELOPMENT

1972 ◽  
Vol 53 (3) ◽  
pp. 662-680 ◽  
Author(s):  
V. Herzog ◽  
F. Miller
Keyword(s):  
Endoplasmic Reticulum ◽  
Postnatal Development ◽  
Rough Endoplasmic Reticulum ◽  
Lacrimal Gland ◽  
Peroxidase Activity ◽  
Secretory Granules ◽  
Acinar Cells ◽  
Secretory Cells ◽  
Lacrimal Fluid ◽  
Endogenous Peroxidase

The distribution of endogenous peroxidase activity in the lacrimal gland of the rat during postnatal development was investigated by electron microscope cytochemistry Peroxidase activity is first found 6 hr after birth in only a few acinar cells At this stage, reaction product fills only localized segments of the scant rough endoplasmic reticulum and of the perinuclear cisternae. Peroxidase activity thus develops asynchronously in a given cell as well as in the secretory cell population as a whole 2 days after birth, all cisternae of the rough endoplasmic reticulum of a peroxidase-positive cell contain reaction product, but the majority of the acinar cells is still negative During the next days, the number of peroxidase-positive cells and the amount of the rough endoplasmic reticulum increase rapidly. By 15 days postparturition, all secretory cells are peroxidase-positive. Reaction product is then found in all cisternae of the rough endoplasmic reticulum including the perinuclear cisternae, in smooth surface vesicles located mainly between the rough endoplasmic reticulum and the Golgi stacks, in condensing vacuoles, and in all secretory granules The Golgi cisternae rarely contain reaction product In total homogenates and in fractions of glandular tissue of adult rats, peroxidatic and catalatic activities are demonstrable. The microsomal fractions and the postmicrosomal supernatants were used to separate peroxidase from catalase by precipitation with ammonium sulfate, and the following parameters were determined: substrate (H2O2-) optimum (∼ 2.0 x 10-4M), pH-optimum (pH 6 5), temperature-optimum (42°C), and the absorption maximum (415 nm before and 425 nm after addition of H2O2) The same parameters were obtained from lacrimal fluid peroxidase. Both peroxidase from lacrimal gland and that from lacrimal fluid are almost completely inhibited by 10-3M aminotriazole and are possibly identical enzymes. Peroxidase is secreted into lacrimal fluid, which does not contain catalase.

Studies on Pogonophora

1969 ◽  
Vol 51 (3) ◽  
pp. 759-773
Author(s):  
COLIN LITTLE ◽  
BRIJ L. GUPTA
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Oxygen Consumption ◽  
Rough Endoplasmic Reticulum ◽  
Concentration Factor ◽  
Organic Molecules ◽  
Secretory Cells

1. The oxygen consumption of S. ekmani at 5° C is 0.06 µl./mg./hr. 2. Phenylalanine and glycine are concentrated by S. ekmani. The concentration factor reaches a maximum after 30 min. in animals removed from their tubes. In animals inside their tubes, the rate of uptake is limited by the rate of diffusion through the walls of the tube. The phenylalanine does not move appreciably into the alcohol-insoluble extract of the animals over a period of 1 hr. 3. Protein is taken up by S. ekmani when animals are removed from their tubes. Uptake is slower than uptake of amino acids, and may involve a different mechanism. 4. Autoradiography using S. mergophorum shows that phenylalanine is not adsorbed on the cuticle. It is found especially in secretory cells, within which it is localized over rough endoplasmic reticulum, Golgi regions and secretion spherules. 5. The site and mechanism of uptake of organic molecules are discussed; and the types of molecules absorbed, together with the significance these may have in the overall metabolism, are considered.

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