A Model of Pattern Formation in Insect Embryogenesis

1977 ◽  
Vol 23 (1) ◽  
pp. 117-139
Author(s):  
H. MEINHARDT
Keyword(s):  
Positional Information ◽  
Posterior Pole ◽  
Morphogen Gradient ◽  
Insect Development ◽  
High Concentration ◽  
Insect Embryogenesis ◽  
Anterior Side ◽  
First Pass ◽  
Pass Through ◽  
The Individual

A model is proposed in which the interaction of an autocatalytic substance with a short diffusion range - the activator - and its more diffusible antagonist - the inhibitor - leads to a local high concentration of activator at the posterior pole of the egg. The inhibitor, which is then produced mainly in this activated region, diffuses into the rest of the egg, where it acts as a ‘morphogen’, forming a concentration gradient which supplies positional information. This model can account quantitatively for the patterns resulting from a large number of different experiments performed during early insect development, including ligation, u.v.-irradiation and microsurgical manipulations. The formation of additional posterior structures is interpreted as the result of the appearance of a new activator peak. Omission of segments after ligation of the egg is explained as the result of accumulation of morphogen (the inhibitor) at the posterior side of the ligation and a decrease of morphogen on the anterior side. In order to account for certain quantitative features of the ligation experiments it is necessary to assume that determination in response to the morphogen gradient is a slow, stepwise process, in which the nuclei or cells first pass through determination stages characteristic for more anterior structures until they ultimately form a given structure. The mutual interactions of activator and inhibitor are expressed as a set of partial differential equations. The individual experiments are simulated by solving these equations by use of a computer.

An Evaluation of a Plasma Fractionation System

1960 ◽  
Vol 4 (01) ◽  
pp. 031-044
Author(s):  
George Y. Shinowara ◽  
E. Mary Ruth
Keyword(s):  
Biological Activity ◽  
Ionic Strength ◽  
Plasma Proteins ◽  
High Concentration ◽  
Significant Evidence ◽  
Native Proteins ◽  
Mobility Data ◽  
Fractionation Procedure ◽  
The Individual ◽  
Low Ionic Strength

SummaryFour primary fractions comprising at least 97 per cent of the plasma proteins have been critically appraised for evidence of denaturation arising from a low temperature—low ionic strength fractionation system. The results in addition to those referable to the recovery of mass and biological activity include the following: The high solubilities of these fractions at pH 7.3 and low ionic strengths; the compatibility of the electrophoretic and ultracentrifugal data of the individual fractions with those of the original plasma; and the recovery of hemoglobin, not hematin, in fraction III obtained from specimens contaminated with this pigment. However, the most significant evidence for minimum alterations of native proteins was that the S20, w and the electrophoretic mobility data on the physically recombined fractions were identical to those found on whole plasma.The fractionation procedure examined here quantitatively isolates fibrinogen, prothrombin and antithrombin in primary fractions. Results have been obtained demonstrating its significance in other biological systems. These include the following: The finding of 5 S20, w classes in the 4 primary fractions; the occurrence of more than 90 per cent of the plasma gamma globulins in fraction III; the 98 per cent pure albumin in fraction IV; and, finally, the high concentration of beta lipoproteins in fraction II.

scholarly journals A Synergistic Anti-Diabetic Effect by Ginsenosides Rb1 and Rg3 through Adipogenic and Insulin Signaling Pathways in 3T3-L1 Cells

2021 ◽  
Vol 11 (4) ◽  
pp. 1725
Author(s):  
Hee-Do Hong ◽  
Sun-Il Choi ◽  
Ok-Hwan Lee ◽  
Young-Cheul Kim
Keyword(s):  
Transcription Factors ◽  
Insulin Signaling ◽  
Adipocyte Differentiation ◽  
Red Ginseng ◽  
Inhibitory Effects ◽  
Rt Pcr ◽  
High Concentration ◽  
Expression Of Genes ◽  
The Individual ◽  
Maximal Expression

Although ginsenosides Rb1 and Rg3 have been identified as the significant ginsenosides found in red ginseng that confer anti-diabetic actions, it is unclear whether insulin-sensitizing effects are mediated by the individual compounds or by their combination. To determine the effect of ginsenosides Rb1 and Rg3 on adipocyte differentiation, 3T3-L1 preadipocytes were induced to differentiate the standard hormonal inducers in the absence or presence of ginsenosides Rb1 or Rg3. Additionally, we determined the effects of Rb1, Rg3, or their combination on the expression of genes related to adipocyte differentiation, adipogenic transcription factors, and the insulin signaling pathway in 3T3-L1 cells using semi-quantitative RT-PCR. Rb1 significantly increased the expression of CEBPα, PPARγ, and aP2 mRNAs. However, Rg3 exerted its maximal stimulatory effect on these genes at 1 μM concentration, while a high concentration (50 μM) showed inhibitory effects. Similarly, treatment with Rb1 and Rg3 (1 μM) increased the expression of IRS-1, Akt, PI3K, GLUT4, and adiponectin. Importantly, co-treatment of Rb1 and Rg3 (9:1) induced the maximal expression levels of these mRNAs. Our data indicate that the anti-diabetic activity of red ginseng is, in part, mediated by synergistic actions of Rb1 and Rg3, further supporting the significance of minor Rg3.

scholarly journals Lymphocytic Choriomeningitis Virus Alters the Expression of Male Mouse Scent Proteins

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1180
Author(s):  
Michael B. A. Oldstone ◽  
Brian C. Ware ◽  
Amanda Davidson ◽  
Mark C. Prescott ◽  
Robert J. Beynon ◽  
...  
Keyword(s):  
Mate Selection ◽  
Lymphocytic Choriomeningitis ◽  
T Cell Response ◽  
Mature Male ◽  
Cytotoxic T Cell ◽  
Scent Marks ◽  
High Concentration ◽  
Embryo Survival ◽  
Major Urinary Proteins ◽  
The Individual

Mature male mice produce a particularly high concentration of major urinary proteins (MUPs) in their scent marks that provide identity and status information to conspecifics. Darcin (MUP20) is inherently attractive to females and, by inducing rapid associative learning, leads to specific attraction to the individual male’s odour and location. Other polymorphic central MUPs, produced at much higher abundance, bind volatile ligands that are slowly released from a male’s scent marks, forming the male’s individual odour that females learn. Here, we show that infection of C57BL/6 males with LCMV WE variants (v2.2 or v54) alters MUP expression according to a male’s infection status and ability to clear the virus. MUP output is substantially reduced during acute adult infection with LCMV WE v2.2 and when males are persistently infected with LCMV WE v2.2 or v54. Infection differentially alters expression of darcin and, particularly, suppresses expression of a male’s central MUP signature. However, following clearance of acute v2.2 infection through a robust virus-specific CD8 cytotoxic T cell response that leads to immunity to the virus, males regain their normal mature male MUP pattern and exhibit enhanced MUP output by 30 days post-infection relative to uninfected controls. We discuss the likely impact of these changes in male MUP signals on female attraction and mate selection. As LCMV infection during pregnancy can substantially reduce embryo survival and lead to lifelong infection in surviving offspring, we speculate that females use LCMV-induced changes in MUP expression both to avoid direct infection from a male and to select mates able to develop immunity to local variants that will be inherited by their offspring.

scholarly journals Specific Binding of Rubidium in Chlorella

1962 ◽  
Vol 45 (5) ◽  
pp. 959-977 ◽  
Author(s):  
Dan Cohen
Keyword(s):  
Active Transport ◽  
Binding Sites ◽  
Specific Binding ◽  
High Concentration ◽  
External Concentration ◽  
Specific Binding Sites ◽  
Dense Suspension ◽  
Concentration Of Ions ◽  
The Individual ◽  
A Site

Specific binding sites for potassium, which may be components of the carriers for active transport for K in Chlorella, were characterized by their capacity to bind rubidium. A dense suspension was allowed to take up Rb86 from a low concentration of Rb86 and a high concentration of ions which saturate non-specific sites. The amount bound was derived from the increase in the external concentration of Rb86 following addition of excess potassium. The sites were heterogeneous. The average affinity of Rb and various other ions for the sites was determined by plotting the degree of displacement of Rb86 against log molar concentration of the individual ions. Interpolation gave the concentration for 50 per cent displacement of Rb, which is inversely related to affinity. The order of affinity was not changed when the cells were frozen, or boiled either in water or in 70 per cent ethanol. The affinity is maximal for ions with a crystalline radius of 1.3 to 1.5 A and a high polarizability, and is not related to the hydrated radius or valency. It is suggested that binding groups in a site are rigidly arranged, the irregular space between them being 2.6 to 3.0 A across, so that affinity is high for ions of this diameter and high polarizability.

Full Well Corrosion Insight – Case Studies in the Added Value of Electromagnetic Thickness Measurements During Well Interventions

2021 ◽  
Author(s):  
Andrew Imrie ◽  
Maciej Kozlowski ◽  
Omar Torky ◽  
Aditya Arie Wijaya
Keyword(s):  
Case Studies ◽  
Wall Thickness ◽  
Added Value ◽  
Metal Loss ◽  
Outer Diameter ◽  
Metal Thickness ◽  
True Condition ◽  
Pass Through ◽  
External Corrosion ◽  
The Individual

AbstractMonitoring pipe corrosion is one of the critical aspects in the well intervention. Such analysis is used to evaluate and justify any remedial actions, to prolong the longevity of the well. Typical corrosion evaluation methods of tubulars consist of multifinger caliper tools that provide high-resolution measurements of the internal condition of the pipe. Routinely, this data is then analyzed and interpreted with respect to the manufacture's nominal specification for each tubular. However, this requires assumptions on the outer diameter of the tubular may add uncertainty, and incorrectly calculate the true metal thicknesses. This paper will highlight cases where the integration of such tool and electromagnetic (EM) thickness data adds value in discovering the true condition of both the first tubular and outer casings.These case studies demonstrate the use of a multireceiver, multitransmitter electromagnetic (EM) metal thickness tool operating at multiple simultaneous frequencies. It is used to measure the individual wall thickness across multiple strings (up to five) and operates continuously, making measurements in the frequency domain. This tool was combined with a multifinger caliper to provide a complete and efficient single-trip diagnosis of the tubing and casing integrity. The combination of multifinger caliper and EM metal thickness tool results gives both internal and external corrosion as well as metal thickness of first and outer tubular strings.The paper highlights multiple case studies including; i) successfully detecting several areas of metal loss (up to greater than 32%) on the outer string, which correlated to areas of the mobile salt formation, ii) overlapping defects in two tubulars and, iii) cases where a multifinger caliper alone doesn't provide an accurate indication of the true wall thickness. The final case highlights the advantages of integrating multiple tubular integrity tools when determining the condition of the casing wall.Metal thickness tools operating on EM principles benefit from a slim outer diameter design that allows the tools to pass through restrictions which typically would prevent ultrasonic scanning thickness tools. Additionally, EM tools are unaffected by the type of fluid in the wellbore and not affected by any non-ferrous scale buildup that may present in the inside of the tubular wall. Combinability between complementary multifinger caliper technology and EM thickness results in two independent sensors to provide a complete assessment of the well architecture.

Memoirs: On the Intracellular Micro-organisms of some Bostrychild Beetles

1934 ◽  
Vol s2-77 (306) ◽  
pp. 243-253
Author(s):  
K. MANSOUR
Keyword(s):  
Alimentary Tract ◽  
Posterior Pole ◽  
Bursa Copulatrix ◽  
Final Destination ◽  
Rhizopertha Dominica ◽  
Pass Through ◽  
Micro Organisms ◽  
Biological Relation

1. Rhizopertha dominica, F., Sinoxylon ceratoniae, L., and Bostrychoplites Zickeli, Mars., possess paired mycetomes full of bacteria-like micro-organisms. 2. The transmission of the micro-organisms from one generation of the host to the next is carried out in the following way: (a) Micro-organisms from the mycetomes invade the testis lobes, multiply, and mix with the sperms. (b) These micro-organisms pass with the sperms during copulation into the bursa copulatrix of the female. (c) From this region they pass through the micropyle of the fully formed eggs during their passage to the outside, and thus the infection is accomplished. 3. The invasion of the testis lobes by micro-organisms causes abnormality in the process of spermatogenesis. 4. The micro-organisms in the developing egg are first seen near the micropylar region in the peripheral protoplasm. In a later stage these micro-organisms, which have multiplied greatly by then, are to be found in between the yolk globules towards the posterior pole of the egg. From this stage onwards the course these micro-organisms follow till they reach their final destination in the larva has not been followed. 5. The mycetomes throughout life remain quite isolated from the alimentary tract. 6. The biological relation between the micro-organisms and their host is also discussed.

Leukocyte kinetics in the human lung: role of exercise and catecholamines

1984 ◽  
Vol 57 (3) ◽  
pp. 711-719 ◽  
Author(s):  
A. L. Muir ◽  
M. Cruz ◽  
B. A. Martin ◽  
H. Thommasen ◽  
A. Belzberg ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Peripheral Blood ◽  
Human Lung ◽  
Work Load ◽  
Low Flow ◽  
Epinephrine Infusion ◽  
First Pass ◽  
Pass Through

In six normal supine subjects epinephrine infusion produced a greater leukocytosis with smaller changes in heart rate and blood pressure than did norepinephrine or isoproterenol. Upright exercise in those subjects produced a greater leukocytosis than supine exercise at the same work load. To determine the lung's participation in these events, indium-labeled neutrophils (PMN) were given to four of the subjects. We found that 20–25% were retained in the first pass through the lung when compared with technetium-labeled erythrocytes. The number of labeled PMN in the lung gradually decreased and the number in the spleen and the liver increased. Exercise and catecholamine infusion caused an acceleration in the release of labeled cells from the lung, an increase in both labeled and unlabeled cells in the peripheral blood, and an increase in the number of labeled cells in the liver and spleen. This suggests that increased perfusion of low-flow areas in the lung may contribute to the increased leukocytosis seen in association with both exercise and catecholamine infusion.

scholarly journals All fingers are not the same: Handling variable-length sequences in a discriminative setting using conformal multi-instance kernels

2017 ◽  
Author(s):  
Sarvesh Nikumbh ◽  
Peter Ebert ◽  
Nico Pfeifer
Keyword(s):  
Binary Classification ◽  
Positional Information ◽  
Weight Vector ◽  
Variable Length ◽  
Genomic Sequences ◽  
String Kernels ◽  
Promoter Sequences ◽  
Novel Approach ◽  
The Individual ◽  
Visualization Techniques

AbstractMost string kernels for comparison of genomic sequences are generally tied to using (absolute) positional information of the features in the individual sequences. This poses limitations when comparing variable-length sequences using such string kernels. For example, profiling chromatin interactions by 3C-based experiments results in variable-length genomic sequences (restriction fragments). Here, exact position-wise occurrence of signals in sequences may not be as important as in the scenario of analysis of the promoter sequences, that typically have a transcription start site as reference. Existing position-aware string kernels have been shown to be useful for the latter scenario.In this work, we propose a novel approach for sequence comparison that enables larger positional freedom than most of the existing approaches, can identify a possibly dispersed set of features in comparing variable-length sequences, and can handle both the aforementioned scenarios. Our approach, CoMIK, identifies not just the features useful towards classification but also their locations in the variable-length sequences, as evidenced by the results of three binary classification experiments, aided by recently introduced visualization techniques. Furthermore, we show that we are able to efficiently retrieve and interpret the weight vector for the complex setting of multiple multi-instance kernels.

scholarly journals Development of the cardinality principle in Macedonian preschool children

Psihologija ◽  
2009 ◽  
Vol 42 (4) ◽  
pp. 459-475 ◽  
Author(s):  
Ana Nikoloska
Keyword(s):  
Preschool Children ◽  
Kindergarten Children ◽  
Pass Through ◽  
The Individual

Cardinality principle refers to the fact that the last number tag used in counting determines the cardinality of a set. Macedonian kindergarten children were tested with the give-a-number task for understanding of this principle. It was found that Macedonian children, unlike their western counterparts, pass through an additional stage, 5-knowers, before they master the cardinality principle. Also, the age at which they pass through the individual stages is somewhat higher than the age of children coming from western samples. Possible explanations are offered and discussed.

Sign in / Sign up

Export Citation Format

Share Document