scholarly journals A Simple Method for the Preparation of Polyacrylamide Gel Filled Capillaries for High Performance Separation of Polynucleotides by Using Capillary Gel Electrophoresis

1991 ◽  
Vol 20 (3) ◽  
pp. 371-374 ◽  
Author(s):  
Yoshinobu Baba ◽  
Toshiko Matsuura ◽  
Kyoko Wakamoto ◽  
Mitsutomo Tsuhako
Keyword(s):  
Gel Electrophoresis ◽  
High Performance ◽  
Polyacrylamide Gel ◽  
Simple Method ◽  
Capillary Gel Electrophoresis

Preparation of polyacrylamide gel filled capillaries for ultrahigh resolution of polynucleotides by capillary gel electrophoresis

1992 ◽  
Vol 64 (11) ◽  
pp. 1221-1225 ◽  
Author(s):  
Yoshinobu. Baba ◽  
Toshiko. Matsuura ◽  
Kyoko. Wakamoto ◽  
Yoshiko. Morita ◽  
Yumi. Nishitsu ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Ultrahigh Resolution ◽  
Capillary Gel Electrophoresis

Analysis of extracellular proteins by native polyacrylamide gel electrophoresis with infiltrated leaf tissue: application to pathogenesis-related proteins

1988 ◽  
Vol 66 (6) ◽  
pp. 1227-1229 ◽  
Author(s):  
Jean Grenier ◽  
François Côté ◽  
Alain Asselin
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Leaf Tissue ◽  
Polyacrylamide Gel ◽  
Simple Method ◽  
Pathogenesis Related Proteins ◽  
Intercellular Fluid ◽  
Pathogenesis Related ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Related Proteins

In addition to polyacrylamide gel electrophoretic analysis of intercellular fluid extracts, a simple method of detection of extracellular pathogenesis-related proteins was based on direct native polyacrylamide gel electrophoresis for acidic and basic proteins with leaf tissue infiltrated with 150 mM sucrose. This technique allowed for the detection of the complete set of tobacco pathogenesis-related proteins without having to extract the intercellular fluid by low-speed centrifugation. A major advantage of the technique is the capacity to observe the distribution of extracellular endogenous or exogenous proteins in the tissue directly subjected to electrophoresis.

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