A Pilot Clinical Study on Thiamine Hydrochloride as a New Mosquito Repellent: Determination of the Minimum Effective Dose on Human Skin

SUMMARYFor the standardisation of adrenalin preparations.(a) The colorimetric method is not reliable with weak or impure solutions.(b) The effect on the pupil of the frog's eye gives uncertain results.(c) The determination of the minimum effective dose on the arterioles of the perfused frog is tedious and uncertain; on an average 0·1 per million produces an effect.(d) The determination of the minimum effective dose in causing a rise on the blood pressure of the atropinised rabbit yields fairly satisfactory results.(e) Adrenalin, suprarenalin, and hemisine all give a precisely similar result, 0·0003 mg. per kilo of body weight, or 0·006 per million, of the rabbit's blood causing a distinct rise in the blood pressure in the rabbit, and 0·012 mg. per kilo of body weight, or 0·24 per million, of the blood causing a rise in the cat.(f) The most satisfactory method is the determination of the dose just sufficient to antagonise 0·6 mg. of nitroglycerin (Parke, Davis & Co.). Of adrenalin, 0·0075 mg. is sufficient.I wish to express my indebtedness to Dr Noël Paton for much help in the preparation of this paper.The expenses of this research were defrayed from a grant made to the Laboratory by Mr J. Francis Mason for investigations on the physiology of the ductless glands.

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Lipolytic and hypocalcemic effects of sheep beta-lipotropic hormone

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y70-110 ◽

1970 ◽

Vol 48 (11) ◽

pp. 762-767 ◽

Cited By ~ 10

Author(s):

M. Chrétien ◽

J. Davignon ◽

M. Lis ◽

P. V. Chari ◽

F. Aubry ◽

...

Keyword(s):

Thyroid Gland ◽

Effective Dose ◽

Chemical Structure ◽

Minimum Effective Dose ◽

Short Term ◽

Hypocalcemic Effect ◽

Plasma Magnesium ◽

Fat Pads

Sheep beta-LPH is a hypophyseal lipolytic hormone with defined chemical structure which has been shown to be hypocalcemic when injected into rabbits. The thyroid gland seems to be essential for production of this hypocalcemic effect. Beta-LPH may be a stimulating hormone for calcitonin-secreting cells. It has no effect on plasma magnesium, cholesterol, triglycerides, and glucose, at least in short-term experiments. When intact epididymal fat pads are used for the determination of the LPH lipolytic effect in vitro, a lower minimum effective dose is found than when small pieces are used.

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Enzymatic Determination of Hydroxysteroids in Human Skin Surface Lipids1

Journal of Investigative Dermatology ◽

10.1038/jid.1963.51 ◽

1963 ◽

Vol 41 (5) ◽

pp. 265-268 ◽

Cited By ~ 1

Author(s):

Thomas J Cook ◽

Allan L Lorincz ◽

Alan R Spector

Keyword(s):

Human Skin ◽

Skin Surface ◽

Enzymatic Determination

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Faculty Opinions recommendation of Up-down determination of the 90% effective dose of phenylephrine for the treatment of spinal anesthesia-induced hypotension in parturients undergoing cesarean delivery.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1556005.1047109 ◽

2010 ◽

Author(s):

Bernard Wittels

Keyword(s):

Effective Dose ◽

Spinal Anesthesia ◽

Cesarean Delivery ◽

Induced Hypotension

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Flexible Potentiometric Sensor System for Non-Invasive Determination of Antioxidant Activity of Human Skin: Application for Evaluating the Effectiveness of Phytocosmetic Products

Chemosensors ◽

10.3390/chemosensors9040076 ◽

2021 ◽

Vol 9 (4) ◽

pp. 76

Author(s):

Aleksey V. Tarasov ◽

Ekaterina I. Khamzina ◽

Maria A. Bukharinova ◽

Natalia Yu. Stozhko

Keyword(s):

Antioxidant Activity ◽

Human Skin ◽

Skin Diseases ◽

Silver Chloride ◽

Potentiometric Sensor ◽

Sensor System ◽

Non Invasive ◽

Pet Film ◽

The Impact

In contemporary bioanalysis, monitoring the antioxidant activity (AOA) of the human skin is used to assess stresses, nutrition, cosmetics, and certain skin diseases. Non-invasive methods for skin AOA monitoring have certain advantages over invasive methods, namely cost-effectiveness, lower labor intensity, reduced risk of infection, and obtaining results in the real-time mode. This study presents a new flexible potentiometric sensor system (FPSS) for non-invasive determination of the human skin AOA, which is based on flexible film electrodes (FFEs) and membrane containing a mediator ([Fe(CN)6]3–/4–). Low-cost available materials and scalable technologies were used for FFEs manufacturing. The indicator FFE was fabricated based on polyethylene terephthalate (PET) film and carbon veil (CV) by single-sided hot lamination. The reference FFE was fabricated based on PET film and silver paint by using screen printing, which was followed by the electrodeposition of precipitate containing a mixture of silver chloride and silver ferricyanide (SCSF). The three-electrode configuration of the FPSS, including two indicator FFEs (CV/PET) and one reference FFE (SCSF/Ag/PET), has been successfully used for measuring the skin AOA and evaluating the impact of phytocosmetic products. FPSS provides reproducible (RSD ≤ 7%) and accurate (recovery of antioxidants is almost 100%) results, which allows forecasting its broad applicability in human skin AOA monitoring as well as for evaluating the effectiveness of topically and orally applied antioxidants.

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