Direct Evidence for the Plant Origin of Brazilian Propolis by the Observation of Honeybee Behavior and Phytochemical Analysis

As a result of accumulated experience from the past generations, today, all the world’s cultures have an extensive knowledge of herbal medicine. In recent years, ethno medicinal studies has received much attention as this brings to light the numerous little known and unknown medicinal virtues especially of plant origin which needs evaluation on modern scientific lines such as phytochemical analysis, pharmacological screening and clinical trials. This review was designed to highlight the traditional uses of medicinal plants.

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Identification of the Plant Origin of Propolis from Jeju Island, Korea, by Observation of Honeybee Behavior and Phytochemical Analysis

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.120580 ◽

2012 ◽

Vol 76 (11) ◽

pp. 2135-2138 ◽

Cited By ~ 3

Author(s):

Kohsuke SHIMOMURA ◽

Saori INUI ◽

Yasumasa SUGIYAMA ◽

Miho KUROSAWA ◽

Jun NAKAMURA ◽

...

Keyword(s):

Jeju Island ◽

Phytochemical Analysis ◽

Plant Origin

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Plant origin of Okinawan propolis: honeybee behavior observation and phytochemical analysis

The Science of Nature ◽

10.1007/s00114-008-0383-y ◽

2008 ◽

Vol 95 (8) ◽

pp. 781-786 ◽

Cited By ~ 47

Author(s):

Shigenori Kumazawa ◽

Jun Nakamura ◽

Masayo Murase ◽

Mariko Miyagawa ◽

Mok-Ryeon Ahn ◽

...

Keyword(s):

Phytochemical Analysis ◽

Plant Origin ◽

Behavior Observation

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Origin and Chemical Variation of Brazilian Propolis

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/neh060 ◽

2005 ◽

Vol 2 (1) ◽

pp. 33-38 ◽

Cited By ~ 145

Author(s):

Antonio Salatino ◽

Érica Weinstein Teixeira ◽

Giuseppina Negri ◽

Dejair Message

Keyword(s):

Chemical Composition ◽

Volatile Compounds ◽

Plant Species ◽

Chemical Variation ◽

Plant Origin ◽

Cinnamic Acids ◽

Pentacyclic Triterpenoids ◽

Ancient Times ◽

Brazilian Propolis ◽

Plant Sources

Propolis is a hive product containing chiefly beeswax and plant-derived substances such as resin and volatile compounds. Propolis has been used as an antiseptic and wound healer since ancient times and interest for the product has increased recently. Probably few plant species contribute as major resin sources. Green propolis derives mainly from vegetative apices ofBaccharis dracunculifolia(alecrim plants). However, wide variation detected in the chemical composition suggests contributions from alternative resin plant sources. Predominant components of the resin of green propolis are cinnamic acids, chiefly compounds bearing prenyl groups. Terpenoid compounds, such as sesqui, di and pentacyclic triterpenoids, have been detected in many, but not all, samples investigated. Propolis research has uncovered potentialities of substances previously isolated from plants and has detected constituents of plant origin that would hardly be known otherwise.

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The Immuno-Electron Microscopic Localization of the Mo-Fe Protein Component of Nitrogenase in Cells of Azotobacter Vinelandii

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073313 ◽

1973 ◽

Vol 31 ◽

pp. 574-575

Author(s):

J. T. Stasny ◽

R. C. Burns ◽

R. W. F. Hardy

Keyword(s):

Cellular Localization ◽

Direct Evidence ◽

Azotobacter Vinelandii ◽

Intracellular Localization ◽

Protein Component ◽

Electron Microscopic ◽

Thin Sections ◽

Fe Protein ◽

Intracytoplasmic Membranes

Structure-functlon studies of biological N2-fixation have correlated the presence of the enzyme nitrogenase with increased numbers of intracytoplasmic membranes in Azotobacter. However no direct evidence has been provided for the internal cellular localization of any nitrogenase. Recent advances concerned with the crystallizatiorTand the electron microscopic characterization of the Mo-Fe protein component of Azotobacter nitrogenase, prompted the use of this purified protein to obtain antibodies (Ab) to be conjugated to electron dense markers for the intracellular localization of the protein by electron microscopy. The present study describes the use of ferritin conjugated to goat antitMo-Fe protein immunoglobulin (IgG) and the observations following its topical application to thin sections of N2-grown Azotobacter.

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Structural similarity of ribosomes from E. coli and A. salina

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082078 ◽

1978 ◽

Vol 36 (2) ◽

pp. 242-243

Author(s):

M. Boublik ◽

R.M. Wydro ◽

W. Hellmann ◽

F. Jenkins

Keyword(s):

Ribosomal Proteins ◽

Direct Evidence ◽

Structural Similarity ◽

Carbon Support ◽

Artemia Salina ◽

Uranyl Acetate ◽

Biological Assays ◽

E Coli ◽

And Function ◽

Fine Carbon

Ribosomes are ribonucleoprotein particles necessary for processing the genetic information of mRNA into proteins. Analogy in composition and function of ribosomes from diverse species, established by biochemical and biological assays, implies their structural similarity. Direct evidence obtained by electron microscopy seems to be of increasing relevance in understanding the structure of ribosomes and the mechanism of their role in protein synthesis.The extent of the structural homology between prokaryotic and eukaryotic ribosomes has been studied on ribosomes of Escherichia coli (E.c.) and Artemia salina (A.s.). Despite the established differences in size and in the amount and proportion of ribosomal proteins and RNAs both types of ribosomes show an overall similarity. The monosomes (stained with 0.5% aqueous uranyl acetate and deposited on a fine carbon support) appear in the electron micrographs as round particles with a diameter of approximately 225Å for the 70S E.c. (Fig. 1) and 260Å for the 80S A.s. monosome (Fig. 2).

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Stress Relaxation and the Formation of Silicides in the Process of the Crystallization of NI-NB Films on SI

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100088804 ◽

1991 ◽

Vol 49 ◽

pp. 898-899

Author(s):

N. Rozhanski ◽

V. Lifshitz

Keyword(s):

Thin Films ◽

Stress Relaxation ◽

Integrated Circuits ◽

Direct Evidence ◽

Alloy Film ◽

Diffusion Barriers ◽

Effective Barrier

Thin films of amorphous Ni-Nb alloys are of interest since they can be used as diffusion barriers for integrated circuits on Si. A native SiO2 layer is an effective barrier for Ni diffusion but it deformation during the crystallization of the alloy film lead to the appearence of diffusion fluxes through it and the following formation of silicides. This study concerns the direct evidence of the action of stresses in the process of the crystallization of Ni-Nb films on Si and the structure of forming NiSi2 islands.

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Point and planar defects in the iron sulfide compounds Fe1-xS

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011221x ◽

1984 ◽

Vol 42 ◽

pp. 434-435

Author(s):

Thao A. Nguyen

Keyword(s):

Low Temperatures ◽

Direct Evidence ◽

Iron Sulfide ◽

Planar Defects ◽

Selective Area ◽

Vacancy Ordering ◽

Different Types ◽

Lattice Images ◽

The Subject ◽

Beam Lattice

It is well known that the large deviations from stoichiometry in iron sulfide compounds, Fe1-xS (0≤x≤0.125), are accommodated by iron vacancies which order and form superstructures at low temperatures. Although the ordering of the iron vacancies has been well established, the modes of vacancy ordering, hence superstructures, as a function of composition and temperature are still the subject of much controversy. This investigation gives direct evidence from many-beam lattice images of Fe1-xS that the 4C superstructure transforms into the 3C superstructure (Fig. 1) rather than the MC phase as previously suggested. Also observed are an intrinsic stacking fault in the sulfur sublattice and two different types of vacancy-ordering antiphase boundaries. Evidence from selective area optical diffractograms suggests that these planar defects complicate the diffraction pattern greatly.

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