scholarly journals Regulation Of Myokine Expression In Exosome-like Vesicles By Electric Pulse Stimulation Of C2C12 Myotubes

2020 ◽  
Vol 52 (7S) ◽  
pp. 927-927
Author(s):  
Ju-Hee Kang ◽  
Sujin Kim ◽  
Sohee Moon ◽  
Hyo-Bum Kwak ◽  
Dong-Ho Park
2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Inkie J.A. Evers-van Gogh ◽  
Sheril Alex ◽  
Rinke Stienstra ◽  
Arjan B. Brenkman ◽  
Sander Kersten ◽  
...  

PLoS ONE ◽  
2010 ◽  
Vol 5 (6) ◽  
pp. e10970 ◽  
Author(s):  
Nathalie Burch ◽  
Anne-Sophie Arnold ◽  
Flurin Item ◽  
Serge Summermatter ◽  
Gesa Brochmann Santana Santos ◽  
...  

2021 ◽  
Vol 16 (3) ◽  
pp. 122-133
Author(s):  
Berezin Sergey Ya. ◽  

The processes of intensification of thread-cutting opera-tions by electric current have been known since the 70s of the last century. Internal threading operations are associated with the mechanism of plastic deformation, and, accordingly, with high values of forces and torques. This reduces the reliability of the process and requires additional measures to reduce power ten-sion. The use of the energy of short stimulating current pulses makes it possible to solve such problems due to the so-called electro-plastic effect (EPE). It is manifested due to additional stresses that cause an increase in the plasticity of metals in the presence of an electric potential and a temperature field in the de-formation center. At present, there is still no clear understanding of the phys-ical processes that determine the change in the VAT of a metal when an electric current is passed. The article presents an analysis of information in this area, which allows us to establish a number of physical phenomena, which are formed into three theories that explain the essence of EPE in metals: electron-dislocation interaction, joule heating, and magnetoplasticity. A description of experiments and equipment for electro-pulse stimulation of the threading operation is presented. The author offers a number of indicators that evaluate the possibilities of electric pulse action on the formation of internal threads when rolling threads, screwing thread-pressing parts, etc. A system of calculated coefficients evaluating the efficiency of the pulse current, its parameters and modes is established.


2007 ◽  
Vol 313 (9) ◽  
pp. 1853-1865 ◽  
Author(s):  
Hideaki Fujita ◽  
Taku Nedachi ◽  
Makoto Kanzaki

2021 ◽  
Vol 11 (21) ◽  
pp. 10436
Author(s):  
Taku Fukushima ◽  
Miho Takata ◽  
Ayano Kato ◽  
Takayuki Uchida ◽  
Takeshi Nikawa ◽  
...  

Exercise has beneficial effects on human health and is affected by two different pathways; motoneuron and endocrine. For the advancement of exercise research, in vitro exercise models are essential. We established two in vitro exercise models using C2C12 myotubes; EPS (electrical pulse stimulation) for a motoneuron model and clenbuterol, a specific β2 adrenergic receptor agonist, treatment for an endocrine model. For clenbuterol treatment, we found that Ppargc1a was induced only in low glucose media (1 mg/mL) using a 1-h treatment of 30 ng/mL clenbuterol. Global transcriptional changes of clenbuterol treatment were analyzed by RNA-seq and gene ontology analyses and indicated that mitogenesis and the PI3K-Akt pathway were enhanced, which is consistent with the effects of exercise. Cxcl1 and Cxcl5 were identified as candidate myokines induced by adrenaline. As for the EPS model, we compared 1 Hz of 1-pulse EPS and 1 Hz of 10-pulse EPS for 24 h and determined Myh gene expressions. Ten-pulse EPS induced higher Myh2 and Myh7 expression. Global transcriptional changes of 10-pulse EPS were also analyzed using RNA-seq, and gene ontology analyses indicated that CaMK signaling and hypertrophy pathways were enhanced, which is also consistent with the effects of exercise. In this paper, we provided two transcriptome results of in vitro exercise models and these databases will contribute to advances in exercise research.


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