Analysis of Bubbles in a Three-Dimensional Fluidized Catalyst Bed Using a Fast X-ray CT Scanner.

Research Highlights: Stand density was connected with wood quality and lumber production to develop a predictive model to better estimate tree value. Background and Objectives: The available standing wood volume in British Columbia (BC), Canada has consistently decreased since 1990. Better understanding the link between stand growth conditions, knot characteristics, the sawmilling process and product quality is essential in making informed forest management decisions and efficiently utilizing wood. The overall objective was to investigate and predict the impact of tree growth as affected by stand density on knot characteristics, lumber volume and value recoveries for two conifer species, two types of sawmills and three economic scenarios. Materials and Methods: Seventy-two amabilis fir and western hemlock trees were harvested from three stands located on Vancouver Island, BC. Sawlogs were scanned using an X-ray computed tomography (CT) scanner and images were processed to extract knot characteristics and reconstruct three-dimensional (3D) log models. The effects of three diameter at breast height (DBH) classes (30, 40 and 50 cm) and three stand densities on knot characteristics, including knot volume, number of knots, average knot area and knot/tree volume ratio, as well as the simulated lumber volume and value recoveries from two types of sawmills (i.e., Coastal and Interior) under three economic scenarios (i.e., baseline, optimistic, and pessimistic) were investigated. Results: As expected, the knot characteristics of both species increased with the DBH. The difference of knot distribution between amabilis fir and western hemlock suggests that the latter is more sensitive to growth site conditions. The sawmilling simulations revealed that the Coastal mill produced a lower lumber volume due to the type of products manufactured and the primary breakdown patterns being used. Conclusions: The developed linear mixed effects models based on the knot characteristics and tree features could predict the value of a standing tree and can be used for estimating preharvest stand value of similar Coastal Hem-Fir forests.

Download Full-text

Three-dimensional imaging of vasculature and parenchyma in intact rodent organs with X-ray micro-CT

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1998.275.3.h1103 ◽

1998 ◽

Vol 275 (3) ◽

pp. H1103-H1114 ◽

Cited By ~ 74

Author(s):

Steven M. Jorgensen ◽

Omer Demirkaya ◽

Erik L. Ritman

Keyword(s):

Tomographic Reconstruction ◽

Three Dimensional ◽

Micro Ct ◽

Reconstruction Algorithms ◽

Ct Scanner ◽

Charge Coupled Device ◽

X Ray ◽

Ccd Detector ◽

Main Components ◽

Thin Crystal

A microcomputed tomography (micro-CT) scanner, which generates three-dimensional (3-D) images consisting of up to a billion cubic voxels, each 5–25 μm on a side, and which has isotropic spatial resolution, is described. Its main components are a spectroscopic X-ray source that produces selectable primary emission peaks at ∼9, 18, or 25 keV and a fluorescing thin crystal plate that is imaged (at selectable magnification) with a lens onto a 2.5 × 2.5-cm, 1,024 × 1,024-pixel, charge-coupled device (CCD) detector array. The specimen is positioned close to the crystal and is rotated in 721 equiangular steps around 360° between each X-ray exposure and its CCD recording. Tomographic reconstruction algorithms, applied to these recorded images, are used to generate 3-D images of the specimen. The system is used to scan isolated, intact, fixed rodent organs (e.g., heart or kidney) with the image contrast of vessel lumens enhanced with contrast medium. 3-D image display and analysis are used to address physiological questions about the internal structure-to-function relationships of the organs.

Download Full-text

Ribosome Structural Organization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051670 ◽

1974 ◽

Vol 32 ◽

pp. 332-333

Author(s):

James A. Lake

Keyword(s):

Ribosomal Proteins ◽

Structural Organization ◽

Three Dimensional ◽

Small Subunit ◽

Structural Studies ◽

X Ray Diffraction ◽

Specific Antibodies ◽

X Ray ◽

E Coli ◽

Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

Download Full-text

3-D reconstruction of molecular shape from microcrystal thin sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077311 ◽

1983 ◽

Vol 41 ◽

pp. 748-749

Author(s):

S. Cusack ◽

J.-C. Jésior

Keyword(s):

Three Dimensional ◽

Molecular Shape ◽

Biological Molecules ◽

Fourier Space ◽

Single Particles ◽

Thin Sections ◽

Standard Methods ◽

X Ray ◽

X Ray Crystallography ◽

Dimensional Reconstruction

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).

Download Full-text

X-ray microtomography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107058 ◽

1988 ◽

Vol 46 ◽

pp. 998-999

Author(s):

H.W. Deckman ◽

B.F. Flannery ◽

J.H. Dunsmuir ◽

K.D' Amico

Keyword(s):

Computed Tomography ◽

Internal Structure ◽

Imaging Technique ◽

Three Dimensional ◽

Tissue Structure ◽

Individual Element ◽

X Ray ◽

Non Invasive ◽

Panoramic Imaging ◽

Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Download Full-text

Revealing gross three-dimensional structure in the SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127712 ◽

1987 ◽

Vol 45 ◽

pp. 656-657

Author(s):

Sterling P. Newberry

Keyword(s):

Freeze Drying ◽

Three Dimensional ◽

Dimensional Structure ◽

Small Object ◽

Final Solution ◽

Dimensional Representation ◽

X Ray ◽

Three Dimensional Representation ◽

Freeze Dried ◽

Critical Point Drying

The beautiful three dimensional representation of small object surfaces by the SEM leads one to search for ways to open up the sample and look inside. Could this be the answer to a better microscopy for gross biological 3-D structure? We know from X-Ray microscope images that Freeze Drying and Critical Point Drying give promise of adequately preserving gross structure. Can we slice such preparations open for SEM inspection? In general these preparations crush more readily than they slice. Russell and Dagihlian got around the problem by “deembedding” a section before imaging. This some what defeats the advantages of direct dry preparation, thus we are reluctant to accept it as the final solution to our problem. Alternatively, consider fig 1 wherein a freeze dried onion root has a window cut in its surface by a micromanipulator during observation in the SEM.

Download Full-text