Golgi body is regulated by COPI vesicle transport via Scyl1 methylation under ER stress

Rab proteins play an essential role in vesicle transport. In particular, RAB1 is thought to participate in the transport of most membrane and secretory proteins. To investigate the role of RAB1 in developing or functioning cells in situ, we constructed transgenic, dominant-negative Rab1 mutants of Drosophila, and examined the protein transport and cellular and subcellular structures of mutant photoreceptor cells. In the transgenic fly, the expression of mutant RAB1 was induced by Gal4 protein, whose expression was triggered by heat treatment (37 degrees C) of the fly. Within several hours after the heat induction, the lumens of the rough endoplasmic reticulum (rER) became swollen, and Golgi bodies were disassembled into vesicle clusters. Corresponding to these changes in cell structure, rhodopsin transport was blocked between the rER and the Golgi body, as indicated by the accumulation of immature rhodopsin carrying a large high-mannose-type oligosaccharide chain. Long-term expression of mutant RAB1 caused the degradation of photoreceptive microvilli and the accumulation of numerous swollen rERs, whereas no distinct changes were found in the axonal regions. These results indicate that, in Drosophila photoreceptor cells, RAB1 contributes to the maintenance of local cell structure by mediating vesicle transport between the rER and Golgi body.

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A facile two-photon fluorescent probe: an endoplasmic reticulum tracker monitoring ER stress and vesicular transport to lysosomes

Chemical Communications ◽

10.1039/c8cc07429a ◽

2019 ◽

Vol 55 (3) ◽

pp. 294-297 ◽

Cited By ~ 6

Author(s):

Pratibha Kumari ◽

Sanjay K. Verma ◽

Shaikh M. Mobin

Keyword(s):

Endoplasmic Reticulum ◽

Er Stress ◽

Fluorescent Probe ◽

Vesicular Transport ◽

Living Cells ◽

Vesicle Transport ◽

Live Cells ◽

Tumor Spheroids ◽

Dynamic Changes ◽

Two Photon

The two-photon fluorescent organic probe ERLp selectively labels the endoplasmic reticulum in live cells and tumor spheroids. ERLp can also monitor ER dynamic changes during ER stress and vesicle transport from the ER to the lysosome in living cells.

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Analysis of the Anterior Secretory Cells of Onchidohs Muricata (Nudibranchia)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099490 ◽

1981 ◽

Vol 39 ◽

pp. 614-615

Author(s):

Roy Skidmore

Keyword(s):

Endoplasmic Reticulum ◽

Secretory Granules ◽

Golgi Body ◽

The Body ◽

Secretory Cells ◽

Secretory Vesicles ◽

High Magnification ◽

Large Numbers ◽

Membrane Bound ◽

Sole Of The Foot

The long-necked secretory cells in Onchidoris muricata are distributed in the anterior sole of the foot. These cells are interspersed among ciliated columnar and conical cells as well as short-necked secretory gland cells. The long-necked cells contribute a significant amount of mucoid materials to the slime on which the nudibranch travels. The body of these cells is found in the subepidermal tissues. A long process extends across the basal lamina and in between cells of the epidermis to the surface of the foot. The secretory granules travel along the process and their contents are expelled by exocytosis at the foot surface.The contents of the cell body include the nucleus, some endoplasmic reticulum, and an extensive Golgi body with large numbers of secretory vesicles (Fig. 1). The secretory vesicles are membrane bound and contain a fibrillar matrix. At high magnification the similarity of the contents in the Golgi saccules and the secretory vesicles becomes apparent (Fig. 2).

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Fine Structure of Planarian Neuroglial Cell

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090920 ◽

1976 ◽

Vol 34 ◽

pp. 188-189 ◽

Cited By ~ 1

Author(s):

Michio Morita ◽

Jay Boyd Best

Keyword(s):

Endoplasmic Reticulum ◽

Ventral Nerve Cord ◽

Osmium Tetroxide ◽

Golgi Body ◽

Nerve Fibers ◽

Neuroglial Cell ◽

Glutaraldehyde Solution ◽

Deep Indentation ◽

Cytoplasmic Processes ◽

Longitudinal Nerve

The species of the planarian Dugesia dorotocephala was used as the experimental animal to study a neuroglial cell in the ventral nerve cord. Animals were fixed with 3% buffered glutaraldehyde solution and postfixed with 1% buffered osmium tetroxide.The neuroglial cell is multipolar, expanding into three or four cytoplasmic processes with many daughter branches. Some neuroglial processes are found to extend perpendicular to the longitudinal nerve fibers, whereas others are seen to be parallel to them. The nucleus of the neuroglial cell is irregular in shape and frequently has a deep indentation. Convex portions of the nucleus seem to be related to the areas from which cytoplasmic processes are extended. Granular endoplasmic reticulum (Fig. 4), Golgi body (Fig. 2), mitochondria (Figs. 1 and 2), microtubules (Fig. 4), and many glycogen granules are observable in the electron dense neuroglial cytoplasm. Neuroglial cells are also observed to contain various sizes of phagosomes and lipids (Fig. 2).

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