Rapid Analysis of Whole Blood and Blood Serum Using near Infrared Spectroscopy

1994 ◽  
Vol 2 (2) ◽  
pp. 67-78 ◽  
Author(s):  
Gyula Domján ◽  
Károly J. Kaffka ◽  
János M. Jákó ◽  
István T. Vályi-Nagy
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Saturation ◽  
Blood Serum ◽  
Whole Blood ◽  
Near Infrared ◽  
Clinical Laboratory ◽  
Analytical Data ◽  
Laboratory Data ◽  
Carbon Dioxide Pressure ◽  
The Relationship

In the present study we describe the relationship between laboratory values obtained with routinely used laboratory analytical methods and near infrared (NIR) spectral data of 126 whole blood and 228 blood serum samples. Spectra were measured with a SPECTRALYZER 1025 (PMC) computerised research analyser. The relationship among laboratory data and values of the second derivative of the log (1/ R) spectra measured at different wavelengths was determined by multiple linear regression (MLR) using three and four term linear summation equations, principal component regression (PCR) and partial least-squares (PLS) regression methods. Along with examples for qualitative detection of protein and lipid in human sera, as well as distinction of albumin and globulin dissolved in physiological saline solution, we describe mathematical models and evaluate their performance for the determination of protein and beta-lipoprotein (β-LP) content of serum as well as oxygen saturation and carbon dioxide pressure in whole blood. Validation of our results yielded a standard error of performance (SEP) of 2.47 g L−1 for protein content and 0.79 TU for β-LP content in blood serum, whereas SEP values of 5.41% for oxygen saturation and 5.27 mm Hg for carbon dioxide pressure in whole blood were found. Our results presented in this preliminary study indicate that NIR measurements can be related to analytical data of whole blood and serum. NIR spectroscopy is a rapid, accurate, cost effective method for determining quality parameters of whole blood and serum and might be a promising new tool in the field of automated clinical laboratory analysis.

Determination of Cholinesterase in Human Blood Using near Infrared Spectroscopy

1998 ◽  
Vol 6 (A) ◽  
pp. A279-A284 ◽  
Author(s):  
Gyula Domján ◽  
János Jákó ◽  
István Vályi-Nagy ◽  
Károly Kaffka
Keyword(s):  
Human Blood ◽  
Near Infrared ◽  
Analytical Data ◽  
Laboratory Data ◽  
Nir Spectroscopy ◽  
Routine Laboratory ◽  
Laboratory Methods ◽  
Wide Range ◽  
Relationship Of ◽  
The Relationship

The level of cholinesterase in the human blood is a very good indicator of liver function. In this study we desrcibe the relationship between cholinesterase values obtained with routine laboratory methods and near infrared (NIR) spectral data of 72 individuals with a wide range of cholinesterase levels. NIR spectra were measured with a SPECTRALYZER 10–25 (PMC) computerised research analyser. The relationship of laboratory data and values of the second derivative of the log (1/ TF) spectra measured at different wavelengths was determined with multiple linear regression (MLR) analysis using three-term linear summation equations. A correlation coefficient ( r) of 0.89 and a standard error of calibration ( SEC) of 987 units L−1 (U/L) of the enzyme were obtained. Our results indicate that NIR measurements of cholinesterase in the human blood serum can be related to the analytical data obtained with routine laboratory methods. NIR spectroscopy is a rapid, accurate, and inexpensive method for determining various constituents in the human blood.

Near-infrared spectroscopy as a possible device for continuous monitoring of arterial carbon dioxide tension during cardiac surgery

Perfusion ◽  
2011 ◽  
Vol 26 (6) ◽  
pp. 524-528 ◽  
Author(s):  
CS Park ◽  
JG Kwak ◽  
C Lee ◽  
C-H Lee ◽  
SK Lee ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Cardiac Surgery ◽  
Infrared Spectroscopy ◽  
Oxygen Saturation ◽  
Continuous Monitoring ◽  
Near Infrared ◽  
Right Hemisphere ◽  
Cerebral Oxygen Saturation ◽  
Cerebral Oxygen ◽  
Regional Cerebral Oxygen Saturation

Background: Insufflation of carbon dioxide (CO2) to the operative field has been used to prevent major organ injury attributed to air embolisms in cardiac surgery. However, it may be preferable to avoid hypercapnia induced by CO2 insufflation, owing to its potentially harmful effect. To investigate the effectiveness of near-infrared spectroscopy (NIRS) as a possible method for continuous monitoring of arterial CO2 tension during cardiac surgery, we evaluated the correlation between the change in arterial CO2 tension and the change in regional cerebral oxygen saturation (rScO2) obtained from NIRS in as controlled a condition as possible. Methods: Thirty patients who underwent surgical correction for atrial or ventricular septal defects were enrolled in this study. Patients who had pulmonary hypertension or other intracardiac anomalies were excluded. Anesthetic and cardiopulmonary bypass (CPB) management were conducted according to our standard institutional practice. Data obtained from arterial blood gas analyses and corresponding regional cerebral oxygen saturation (rScO2) recorded from NIRS before and after the insufflations of CO2 during CPB were used for analysis. Results: The change in arterial CO2 tension correlated with the change in rScO2 in the left hemisphere (r = 0.681, p <0.001, y = −1.393 + 0.547x) and right hemisphere (r = 0.690, p <0.001, y = −1.999 + 0.486x). To control the effects of other variables, including hematocrit and temperature, these relationship were not reduced (left hemisphere: r=0.678, p<0.001; right hemisphere: r=0.634, p<0.001). Conclusions: Since the change in regional cerebral oxygen saturation was correlated with the change in arterial CO2 tension during mild hypothermic CPB, NIRS might be a possible non-invasive method for monitoring of arterial CO2 tension without incurring additional cost in this setting.

The Influence of Carbon Dioxide and Body Position on Near-Infrared Spectroscopic Assessment of Cerebral Hemoglobin Oxygen Saturation

1996 ◽  
Vol 82 (2) ◽  
pp. 278-287 ◽  
Author(s):  
Valerie Pollard ◽  
Donald S. Prough ◽  
A. Eric DeMelo ◽  
Donald J. Deyo ◽  
Tatsuo Uchida ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Saturation ◽  
Near Infrared ◽  
Body Position ◽  
Hemoglobin Oxygen Saturation ◽  
Infrared Spectroscopic

Partial pressure of carbon dioxide in the venous blood of young birds as a predictor of ascites susceptibility in broiler chickens

2010 ◽  
Vol 58 (2) ◽  
pp. 221-230 ◽  
Author(s):  
Mohammad Hassanzadeh ◽  
Mohammad Maddadi ◽  
Sarra Mirzaie ◽  
Keramat Assasie ◽  
Hamid Moayyedian
Keyword(s):  
Carbon Dioxide ◽  
Broiler Chickens ◽  
Venous Blood ◽  
Broiler Breeder ◽  
Fast Growing ◽  
Carbon Dioxide Pressure ◽  
Blood Gas Parameters ◽  
The Relationship ◽  
Slow Growing

In order to investigate the relationship between carbon dioxide tensions in the venous blood of young chickens and ascites susceptibility, one hundred dayold chickens from two pure broiler breeder lines differing in susceptibility to ascites syndrome were obtained and reared at low environmental temperature. Weekly, blood samples were taken for the determination of blood gas parameters and plasma thyroid hormone levels. Dead birds were examined for lesions of ascites daily and all survived birds were autopsied at the end of the trials. In cold conditioning, the cumulative incidence of right ventricular hypertrophy (RVH) and ascites was 78% (39/50) in fast-growing (line A) and 50% (25/50) in slow-growing (line B) chickens, confirming that line A chickens had higher susceptibility to ascites than line B chickens. At 12 days of age, the mean pCO2tension was significantly (P < 0.01) higher in the venous blood of line A than line B chickens. During the experiment, the majority of line A and line B chickens showing RVH and ascites exhibited pCO2tensions equal to, or exceeding 50 mmHg at 12 days of age. Additionally, fast-growing chickens showed significantly lower plasma T3 and T4 concentrations than slow-growing chickens. In conclusion, our data indicate that carbon dioxide pressure in the venous blood of early postnatal broiler chickens could be used as a valuable predictor in the selection of birds susceptible to pulmonary hypertension and ascites. The function of thyroid hormones should also be taken into consideration in further selection procedures for meat-type chickens.

scholarly journals Research of the association of polymorphic variants of the FGB gene (-455G/A, C148T) with average level of fibrinogen in women with reproductive disorders

2018 ◽  
pp. 67-72
Author(s):  
Z. I. Rossokha ◽  
L. P. Sheyko ◽  
N. L. Medvedeva ◽  
N. G. Gorovenko
Keyword(s):  
Blood Plasma ◽  
Clinical Laboratory ◽  
Fibrinogen Level ◽  
Laboratory Data ◽  
Reproductive Disorders ◽  
Reproductive Loss ◽  
Polymorphic Variants ◽  
Hemostatic Disorders ◽  
The Relationship ◽  
Level 2

Fibrinogen is an important factor in blood coagulation. The evaluation of the hemostasis system in women with reproductive disorders is assessed according to fibrinogen level. The purpose of this work was to identify the association between polymorphic variants 455 G/A (rs1800790) and C148T (rs1800787) in the FGB gene and the fibrinogen level in the blood plasma in women with aggravated early reproductive loss or infertility history. The research involved 177 patients. There were studied the relationship between anamnestic, clinical-laboratory data and hemostasis indicators (at the time of treatment) with polymorphic variants of the FGB gene. According to the results of the study, dysfibrinogenemia was observed in 23 % of the examined patients: increased fibrinogen level (≥ 4 g/l) – 8,5 % of patients, decreased fibrinogen level (≤ 2 g/l) –14.5 %. The -455AA genotype and -455AA/148TT genotypes combination for theFGB gene were associated with a decreased level of fibrinogen in women withhypocoagulant hemostatic disorders.

Sign in / Sign up

Export Citation Format

Share Document